Restriction map for
pFN21AB6545
##################################
# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BalI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRV EcoT22I
FseI FspI HaeII HapII HgaI
Hin1I HindIII HpaI HpaII KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 492 GTAC GT^AC
AspHI 864 GAGCAC G[AT]GC[AT]^C
AvaI 96 CTCGGG C^[CT]CG[GA]G
BamHI 964 GGATCC G^GATCC
BanI 712 GGCACC G^G[CT][GA]CC
BclI 680 TGATCA T^GATCA
BlpI 289 GCTCAGC GC^T[ACGT]AGC
BmyI 864 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 289 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 864 GAGCAC G[AT]GC[AT]^C
BsmI 642 GAATGC GAATGC(1/-1)
BsoBI 96 CTCGGG C^[CT]CG[GA]G
Bsp1286I 864 GAGCAC G[AGT]GC[ACT]^C
BssSI 832 CACGAG CACGAG(-5/-1)
BstAPI 56 GCAAAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 289 GCTCAGC GC^T[ACGT]AGC
CfoI 698 GCGC GCG^C
EaeI 667 TGGCCG [CT]^GGCC[GA]
EcoNI 537 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 655 GAATTC G^AATTC
FauI 395 CCCGC CCCGC(4/6)
FbaI 680 TGATCA T^GATCA
HhaI 698 GCGC GCG^C
HinP1I 698 GCGC G^CGC
MspA1I 74 CAGCTG C[AC]G^C[GT]G
PvuII 74 CAGCTG CAG^CTG
RsaI 492 GTAC GT^AC
SfcI 400 CTACAG C^T[GA][CT]AG
TaqI 1053 TCGA T^CGA
TthHB8I 1053 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 396 CCGC CCGC(-3/-1)
1062 CCGC
AcsI 655 GAATTC [GA]^AATT[CT]
781 GAATTT
ApoI 655 GAATTC [GA]^AATT[CT]
781 GAATTT
AseI 556 ATTAAT AT^TAAT
939 ATTAAT
AsnI 556 ATTAAT AT^TAAT
939 ATTAAT
BbsI 272 GTCTTC GAAGAC(2/6)
894 GTCTTC
BpuAI 272 GTCTTC GAAGAC(2/6)
894 GTCTTC
BsaI 766 GAGACC GGTCTC(1/5)
459 GGTCTC
BsmAI 766 GAGAC GTCTC(1/5)
460 GTCTC
BsmFI 773 GTCCC GGGAC(10/14)
888 GTCCC
BspMI 577 ACCTGC ACCTGC(4/8)
918 ACCTGC
BstEII 15 GGTCACC G^GT[ACGT]ACC
324 GGTCACC
BstF5I 61 GGATG GGATG(2/0)
468 GGATG
BstPI 15 GGTCACC G^GT[ACGT]ACC
324 GGTCACC
Cac8I 503 GCAAGC GC[ACGT]^[ACGT]GC
721 GCCTGC
EarI 107 CTCTTC CTCTTC(1/4)
972 GAAGAG
EcoO65I 15 GGTCACC G^GT[ACGT]ACC
324 GGTCACC
EcoT14I 708 CCTTGG C^C[AT][AT]GG
776 CCAAGG
FokI 61 GGATG GGATG(9/13)
468 GGATG
HaeIII 307 GGCC GG^CC
668 GGCC
Ksp632I 107 CTCTTC CTCTTC(1/4)
972 GAAGAG
MaeII 358 ACGT A^CGT
907 ACGT
MseI 557 TTAA T^TAA
940 TTAA
NlaIII 82 CATG CATG^
155 CATG
PleI 270 GAGTC GAGTC(4/5)
1026 GAGTC
PshBI 556 ATTAAT AT^TAAT
939 ATTAAT
SmlI 40 CTCAAG C^T[CT][GA]AG
267 CTTGAG
StyI 708 CCTTGG C^C[AT][AT]GG
776 CCAAGG
Tru9I 557 TTAA T^TAA
940 TTAA
TspRI 303 CAGTG CA[GC]TG[ACGT][ACGT]^
701 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI AlwI BbvI BpmI BsaJI
BseRI BsiYI BslI BsrDI BsrI
BssKI BstNI BstYI DdeI DpnII
EcoRII Fnu4HI HincII HindII HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIV Sau3AI ScrFI SfaNI TfiI
TseI Tsp45I Tsp509I XhoII