(Circular) (Six-base) MAPSORT of: pFN21AB6545.seq Check: 8513 from: 1 to: 5800 pFN21AB6545 5800 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 3863 5247 5515 1632 Size: 467 1764 1384 268 1917 Fragments arranged by size: 1917 1764 1384 467 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5800 AlwNI CAG_nnn'CTG Cuts at: 3543 5115 3543 Size: 1572 4228 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 2660 2786 3109 3335 3487 5596 1493 Size: 1167 126 323 226 152 2109 1697 Fragments arranged by size: 2109 1697 1167 323 226 152 126 AseI AT'TA_AT Cuts at: 160 1032 2562 2945 4354 160 Size: 872 1530 383 1409 1606 Fragments arranged by size: 1606 1530 1409 872 383 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 2101 3125 1870 Size: 73 158 1024 4545 Fragments arranged by size: 4545 1024 158 73 BamHI G'GATC_C Cuts at: 2969 3130 3497 2969 Size: 161 367 5272 Fragments arranged by size: 5272 367 161 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2717 3121 4576 618 Size: 325 217 627 930 404 1455 1842 Fragments arranged by size: 1842 1455 930 627 404 325 217 BanII G_rGCy'C Cuts at: 1391 3119 1391 Size: 1728 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2270 2892 928 Size: 971 371 622 3836 Fragments arranged by size: 3836 971 622 371 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3991 4025 1903 Size: 34 50 34 1970 34 3678 Fragments arranged by size: 3678 1970 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 2685 1548 Size: 1137 4663 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4411 136 Size: 107 122 71 631 605 2739 1525 Fragments arranged by size: 2739 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 2295 3217 2295 Size: 922 4878 Bme1580I G_kGCm'C Cuts at: 1790 3861 5017 1790 Size: 2071 1156 2573 Fragments arranged by size: 2573 2071 1156 BmgBI CAC'GTC Cuts at: 1318 2913 1318 Size: 1595 4205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2890 3531 4489 Size: 416 53 83 399 24 1473 641 958 Cuts at: 4489 442 Size: 1753 Fragments arranged by size: 1753 1473 958 641 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2236 2236 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2452 2880 3098 4460 1239 Size: 1106 107 428 218 1362 2579 Fragments arranged by size: 2579 1362 1106 428 218 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2030 2293 3925 4790 5088 2030 Size: 263 1632 865 298 2742 Fragments arranged by size: 2742 1632 865 298 263 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2470 2765 4463 882 Size: 617 971 295 1698 2219 Fragments arranged by size: 2219 1698 971 617 295 BsaAI yAC'GTr Cuts at: 493 1204 3591 493 Size: 711 2387 2702 Fragments arranged by size: 2702 2387 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3607 3989 4624 275 Size: 53 83 186 936 2074 382 635 1451 Fragments arranged by size: 2074 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2750 2780 1178 Size: 30 165 30 1347 30 4198 Fragments arranged by size: 4198 1347 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2033 2564 2758 1186 Size: 838 9 531 194 4228 Fragments arranged by size: 4228 838 531 194 9 BseYI C'CCAG_C Cuts at: 2008 2607 5003 5499 2008 Size: 599 2396 496 2309 Fragments arranged by size: 2396 2309 599 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5227 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3250 4011 5039 1540 Size: 275 186 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2873 3861 3946 5017 2873 Size: 988 85 1071 3656 Fragments arranged by size: 3656 1071 988 85 BsmI GAATG_Cn' Cuts at: 2653 3311 3404 2653 Size: 658 93 5049 Fragments arranged by size: 5049 658 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2873 3119 3861 3946 5017 1391 Size: 399 1083 246 742 85 1071 2174 Fragments arranged by size: 2174 1083 1071 742 399 246 85 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2591 2932 3155 844 Size: 1747 341 223 3489 Fragments arranged by size: 3489 1747 341 223 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2154 2485 2593 4295 4469 65 Size: 1183 906 331 108 1702 174 1396 Fragments arranged by size: 1702 1396 1183 906 331 174 108 BsrFI r'CCGG_y Cuts at: 1700 1810 4444 4631 1700 Size: 110 2634 187 2869 Fragments arranged by size: 2869 2634 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 2837 3860 4872 2837 Size: 1023 1012 3765 Fragments arranged by size: 3765 1023 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2067 794 Size: 1273 4527 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 2020 2329 1184 Size: 836 309 4655 Fragments arranged by size: 4655 836 309 BstYI r'GATC_y Cuts at: 1972 2421 2815 2969 3130 3497 3882 3899 5351 Size: 449 394 154 161 367 385 17 1452 Cuts at: 5351 5437 5449 5795 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 449 394 385 367 346 161 154 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5248 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 3316 4110 4130 3316 Size: 794 20 4986 Fragments arranged by size: 4986 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2672 3247 4136 Size: 54 1425 50 275 18 842 575 889 Cuts at: 4136 5765 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 842 575 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3247 1537 Size: 275 1435 4090 Fragments arranged by size: 4090 1435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 2972 3730 1353 Size: 765 854 758 3423 Fragments arranged by size: 3423 854 765 758 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 4761 4907 387 Size: 1064 138 2798 374 146 1280 Fragments arranged by size: 2798 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2345 2452 2880 3098 3863 4460 Size: 393 467 246 107 428 218 765 597 Cuts at: 4460 5247 5515 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 765 597 467 428 393 268 246 218 107 EcoNI CCTnn'n_nnAGG Cuts at: 2546 2546 Size: 5800 EcoRI G'AATT_C Cuts at: 2660 3109 3487 2660 Size: 449 378 4973 Fragments arranged by size: 4973 449 378 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 4947 1678 Size: 3269 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2750 2782 4441 4473 4515 Size: 32 135 32 1178 32 1659 32 42 Cuts at: 4515 4547 1373 Size: 32 2626 Fragments arranged by size: 2626 1659 1178 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2149 2557 3035 3144 3390 669 Size: 988 281 211 408 478 109 246 3079 Fragments arranged by size: 3079 988 478 408 281 246 211 109 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4914 5098 1305 Size: 3609 184 2007 Fragments arranged by size: 3609 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3978 Fragments arranged by size: 3978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3758 4117 4276 518 Size: 648 2592 359 159 2042 Fragments arranged by size: 2592 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2081 3216 3893 5041 5286 1606 Size: 475 1135 677 1148 245 2120 Fragments arranged by size: 2120 1148 1135 677 475 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5248 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 3158 4703 1132 Size: 97 1929 1545 2229 Fragments arranged by size: 2229 1929 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5800 PfoI T'CCnGG_A Cuts at: 2424 2857 2957 2972 2424 Size: 433 100 15 5252 Fragments arranged by size: 5252 433 100 15 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2522 2554 2522 Size: 32 5768 PstI C_TGCA'G Cuts at: 830 3152 830 Size: 2322 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 PvuII CAG'CTG Cuts at: 2081 2081 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SfcI C'TryA_G Cuts at: 826 1045 2405 3148 4283 4964 5155 826 Size: 219 1360 743 1135 681 191 1471 Fragments arranged by size: 1471 1360 1135 743 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 2272 3904 4805 5067 820 Size: 197 926 102 227 1632 901 262 1553 Fragments arranged by size: 1632 1553 926 901 262 227 197 102 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5800 StyI C'CwwG_G Cuts at: 513 1065 2713 2781 3239 513 Size: 552 1648 68 458 3074 Fragments arranged by size: 3074 1648 552 458 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3827 3844 3997 4182 4627 325 Size: 1255 2247 17 153 185 445 1498 Fragments arranged by size: 2247 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4046 5758 96 Size: 274 80 33 51 1443 2069 1712 138 Fragments arranged by size: 2069 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2978 3010 5760 5792 2978 Size: 32 2750 32 2986 Fragments arranged by size: 2986 2750 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI