Restriction map for
pFN21AB6545
(Linear) (Six-base) MAPSORT of: KIBB6545.seq Check: 7151 from: 1 to: 1095
KIBB6545.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1095
Size: 94 1001
ApoI r'AATT_y
Cuts at: 0 655 781 1095
Size: 655 126 314
Fragments arranged by size:
655 314 126
AseI AT'TA_AT
Cuts at: 0 557 940 1095
Size: 557 383 155
Fragments arranged by size:
557 383 155
AvaI C'yCGr_G
Cuts at: 0 96 1095
Size: 96 999
BamHI G'GATC_C
Cuts at: 0 964 1095
Size: 964 131
BanI G'GyrC_C
Cuts at: 0 712 1095
Size: 712 383
BbsI GAAGACnn'nnnn_
Cuts at: 0 265 887 1095
Size: 265 622 208
Fragments arranged by size:
622 265 208
BclI T'GATC_A
Cuts at: 0 680 1095
Size: 680 415
BlpI GC'TnA_GC
Cuts at: 0 290 1095
Size: 290 805
BmgBI CAC'GTC
Cuts at: 0 908 1095
Size: 908 187
BmrI ACTGGGnnnn_n'
Cuts at: 0 885 1095
Size: 885 210
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 231 1095
Size: 231 864
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 447 875 1093 1095
Size: 340 107 428 218 2
Fragments arranged by size:
428 340 218 107 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 288 1095
Size: 25 263 807
Fragments arranged by size:
807 263 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 465 760 1095
Size: 465 295 335
Fragments arranged by size:
465 335 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 745 775 1095
Size: 745 30 320
Fragments arranged by size:
745 320 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 28 559 753 1095
Size: 19 9 531 194 342
Fragments arranged by size:
531 342 194 19 9
BseYI C'CCAG_C
Cuts at: 0 3 602 1095
Size: 3 599 493
Fragments arranged by size:
599 493 3
BsiHKAI G_wGCw'C
Cuts at: 0 868 1095
Size: 868 227
BsmI GAATG_Cn'
Cuts at: 0 648 1095
Size: 648 447
Bsp1286I G_dGCh'C
Cuts at: 0 868 1095
Size: 868 227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 586 927 1095
Size: 586 341 168
Fragments arranged by size:
586 341 168
BsrDI GCAATG_nn'
Cuts at: 0 149 480 588 1095
Size: 149 331 108 507
Fragments arranged by size:
507 331 149 108
BssSI C'ACGA_G
Cuts at: 0 832 1095
Size: 832 263
BstAPI GCAn_nnn'nTGC
Cuts at: 0 62 1095
Size: 62 1033
BstEII G'GTnAC_C
Cuts at: 0 15 324 1095
Size: 15 309 771
Fragments arranged by size:
771 309 15
BstYI r'GATC_y
Cuts at: 0 416 810 964 1095
Size: 416 394 154 131
Fragments arranged by size:
416 394 154 131
EaeI y'GGCC_r
Cuts at: 0 667 1095
Size: 667 428
EarI CTCTTCn'nnn_
Cuts at: 0 113 967 1095
Size: 113 854 128
Fragments arranged by size:
854 128 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 340 447 875 1093 1095
Size: 94 246 107 428 218 2
Fragments arranged by size:
428 246 218 107 94 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 541 1095
Size: 541 554
EcoRI G'AATT_C
Cuts at: 0 655 1095
Size: 655 440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 745 777 1095
Size: 745 32 318
Fragments arranged by size:
745 318 32
HincII GTy'rAC
Cuts at: 0 144 552 1030 1095
Size: 144 408 478 65
Fragments arranged by size:
478 408 144 65
MspA1I CmG'CkG
Cuts at: 0 76 1095
Size: 76 1019
PfoI T'CCnGG_A
Cuts at: 0 419 852 952 967 1095
Size: 419 433 100 15 128
Fragments arranged by size:
433 419 128 100 15
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 517 549 1095
Size: 517 32 546
Fragments arranged by size:
546 517 32
PvuII CAG'CTG
Cuts at: 0 76 1095
Size: 76 1019
SfcI C'TryA_G
Cuts at: 0 400 1095
Size: 400 695
SmlI C'TyrA_G
Cuts at: 0 40 267 1095
Size: 40 227 828
Fragments arranged by size:
828 227 40
StyI C'CwwG_G
Cuts at: 0 708 776 1095
Size: 708 68 319
Fragments arranged by size:
708 319 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 973 1005 1095
Size: 973 32 90
Fragments arranged by size:
973 90 32
Enzymes that do cut:
AcuI ApoI AseI AvaI BamHI BanI BbsI BclI
BlpI BmgBI BmrI BplI BpmI BpuEI BsaI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI
BstAPI BstEII BstYI EaeI EarI Eco57MI EcoNI EcoRI
Hin4I HincII MspA1I PfoI PsrI PvuII SfcI SmlI
StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AsiSI AvrII BaeI BanII BbeI BbvCI BcgI
BciVI BfrBI BglI BglII Bme1580I BmtI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstBI BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI