Restriction map for
pFN21AB6915
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII AccI AccIII AclI AcsI
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
Asp700I AspEI AspHI AspI AvaI
AvaII AviII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BglI BglII BlpI BpmI Bpu1102I
BsaBI BsaI BseAI BsgI BsiHKAI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssSI Bst1107I
BstBI BstEII BstF5I BstNI BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT22I FauI FbaI
FokI FseI FspI HaeII HincII
HindII HindIII HpaI KasI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI MvaI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 309 GGTACC G^GTACC
AccII 18 CGCG CG^CG
AciI 55 GCGG CCGC(-3/-1)
AcyI 94 GACGCC G[GA]^CG[CT]C
AflII 319 CTTAAG C^TTAAG
AlwI 583 GGATC GGATC(4/5)
ApaI 10 GGGCCC GGGCC^C
AseI 351 ATTAAT AT^TAAT
AsnI 351 ATTAAT AT^TAAT
Asp718I 309 GGTACC G^GTACC
AvrII 174 CCTAGG C^CTAGG
BanI 309 GGTACC G^G[CT][GA]CC
BanII 10 GGGCCC G[GA]GC[CT]^C
BbvI 473 GCTGC GCAGC(8/12)
BfrI 319 CTTAAG C^TTAAG
BlnI 174 CCTAGG C^CTAGG
BsaAI 112 TACGTG [CT]AC^GT[GA]
BsaHI 94 GACGCC G[GA]^CG[CT]C
BsaWI 312 ACCGGA [AT]^CCGG[AT]
BsiEI 191 CGGCCG CG[GA][CT]^CG
BspHI 77 TCATGA T^CATGA
BssHII 17 GCGCGC G^CGCGC
BstAPI 62 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 18 CGCG CG^CG
DsaI 152 CCGTGG C^C[GA][CT]GG
EaeI 191 CGGCCG [CT]^GGCC[GA]
EagI 191 CGGCCG C^GGCCG
EclXI 191 CGGCCG C^GGCCG
Eco52I 191 CGGCCG C^GGCCG
EcoT14I 174 CCTAGG C^C[AT][AT]GG
Fnu4HI 473 GCTGC GC^[ACGT]GC
Hin1I 94 GACGCC G[GA]^CG[CT]C
HinfI 179 GACTC G^A[ACGT]TC
HphI 149 TCACC GGTGA(8/7)
ItaI 473 GCTGC GC^[ACGT]GC
KpnI 309 GGTACC GGTAC^C
MaeIII 148 GTCAC ^GT[ACGT]AC
MvnI 18 CGCG CG^CG
NspI 469 GCATGC [GA]CATG^[CT]
PleI 179 GACTC GAGTC(4/5)
PshAI 240 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 351 ATTAAT AT^TAAT
RcaI 77 TCATGA T^CATGA
SfaNI 24 GATGC GCATC(5/9)
SphI 469 GCATGC GCATG^C
StuI 220 AGGCCT AGG^CCT
StyI 174 CCTAGG C^C[AT][AT]GG
TaqI 128 TCGA T^CGA
TseI 473 GCTGC G^C[AT]GC
Tsp45I 148 GTCAC ^GT[GC]AC
Tsp509I 609 AATT ^AATT
TspRI 564 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 128 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AluI 82 AGCT AG^CT
492 AGCT
BcnI 13 CCCGG CC^[GC]GG
102 CCCGG
BfaI 175 CTAG C^TAG
451 CTAG
BmyI 10 GGGCCC G[AGT]GC[ACT]^C
115 GTGCCC
BsaJI 152 CCGTGG C^C[ACGT][ACGT]GG
174 CCTAGG
BseRI 106 GAGGAG GAGGAG(10/8)
301 GAGGAG
Bsp1286I 10 GGGCCC G[AGT]GC[ACT]^C
115 GTGCCC
BssKI 13 CCCGG ^CC[ACGT]GG
102 CCCGG
CfoI 17 GCGC GCG^C
19 GCGC
Cfr13I 10 GGGCC G^G[ACGT]CC
11 GGCCC
DdeI 217 CTGAG C^T[ACGT]AG
362 CTCAG
DpnII 370 GATC ^GATC
584 GATC
HgaI 94 GACGC GACGC(5/10)
146 GCGTC
HhaI 17 GCGC GCG^C
19 GCGC
HinP1I 17 GCGC G^CGC
19 GCGC
MaeI 175 CTAG C^TAG
451 CTAG
MaeII 67 ACGT A^CGT
113 ACGT
MboI 370 GATC ^GATC
584 GATC
NciI 13 CCCGG CC^[GC]GG
102 CCCGG
NdeII 370 GATC ^GATC
584 GATC
NlaIII 78 CATG CATG^
470 CATG
NlaIV 10 GGGCCC GG[ACGT]^[ACGT]CC
309 GGTACC
Sau3AI 370 GATC ^GATC
584 GATC
Sau96I 10 GGGCC G^G[ACGT]CC
11 GGCCC
ScrFI 13 CCCGG CC^[ACGT]GG
102 CCCGG
SmlI 28 CTCAAG C^T[CT][GA]AG
319 CTTAAG
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AfaI BbsI BpuAI Cac8I HaeIII
HapII HpaII MboII MnlI MseI
MspI MwoI RsaI Tru9I