(Circular) (Six-base) MAPSORT of: pFN21AB0434.seq Check: 1068 from: 1 to: 7303 pFN21AB0434 7303 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5113 278 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4646 1937 Size: 2709 4594 Acc65I G'GTAC_C Cuts at: 4624 4624 Size: 7303 AclI AA'CG_TT Cuts at: 1563 5430 5803 1563 Size: 3867 373 3063 Fragments arranged by size: 3867 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2255 2411 3408 4602 5366 6750 7018 1632 Size: 623 156 997 1194 764 1384 268 1917 Fragments arranged by size: 1917 1384 1194 997 764 623 268 156 AflII C'TTAA_G Cuts at: 820 1017 2353 3518 820 Size: 197 1336 1165 4605 Fragments arranged by size: 4605 1336 1165 197 AflIII A'CryG_T Cuts at: 1203 3762 4406 6202 7179 1203 Size: 2559 644 1796 977 1327 Fragments arranged by size: 2559 1796 1327 977 644 AgeI A'CCGG_T Cuts at: 6134 6134 Size: 7303 AhdI GACnn_n'nnGTC Cuts at: 6032 6032 Size: 7303 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7303 AlwNI CAG_nnn'CTG Cuts at: 4580 5046 6618 4580 Size: 466 1572 5265 Fragments arranged by size: 5265 1572 466 ApaLI G'TGCA_C Cuts at: 5360 6516 5360 Size: 1156 6147 ApoI r'AATT_y Cuts at: 1493 2096 2126 2522 2838 2953 3436 3503 3980 Size: 603 30 396 316 115 483 67 477 Cuts at: 3980 4249 4276 4470 4612 4838 4990 7099 1493 Size: 269 27 194 142 226 152 2109 1697 Fragments arranged by size: 2109 1697 603 483 477 396 316 269 226 194 152 142 115 67 30 27 AseI AT'TA_AT Cuts at: 160 1032 3224 5857 160 Size: 872 2192 2633 1606 Fragments arranged by size: 2633 2192 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 AvaI C'yCGr_G Cuts at: 1870 1943 4628 1870 Size: 73 2685 4545 Fragments arranged by size: 4545 2685 73 AvrII C'CTAG_G Cuts at: 4444 4444 Size: 7303 BamHI G'GATC_C Cuts at: 4633 5000 4633 Size: 367 6936 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2878 4624 6079 618 Size: 325 217 627 1091 1746 1455 1842 Fragments arranged by size: 1842 1746 1455 1091 627 325 217 BanII G_rGCy'C Cuts at: 1391 3811 4622 1391 Size: 2420 811 4072 Fragments arranged by size: 4072 2420 811 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2971 928 Size: 971 1072 5260 Fragments arranged by size: 5260 1072 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2699 2733 5494 5528 1903 Size: 34 50 34 678 34 2761 34 3678 Fragments arranged by size: 3678 2761 678 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5195 6405 5195 Size: 1210 6093 BclI T'GATC_A Cuts at: 1548 1548 Size: 7303 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2887 5914 Size: 107 122 71 631 605 337 878 3027 Cuts at: 5914 136 Size: 1525 Fragments arranged by size: 3027 1525 878 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2389 3693 7298 2389 Size: 1304 3605 2394 Fragments arranged by size: 3605 2394 1304 BlpI GC'TnA_GC Cuts at: 4720 4720 Size: 7303 Bme1580I G_kGCm'C Cuts at: 1790 3481 5364 6520 1790 Size: 1691 1883 1156 2573 Fragments arranged by size: 2573 1883 1691 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2785 5034 5992 Size: 416 53 83 399 24 1368 2249 958 Cuts at: 5992 442 Size: 1753 Fragments arranged by size: 2249 1753 1368 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4385 5963 1239 Size: 3146 1578 2579 Fragments arranged by size: 3146 2579 1578 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2077 2414 2792 5428 6293 6591 2077 Size: 337 378 2636 865 298 2789 Fragments arranged by size: 2789 2636 865 378 337 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2621 5966 882 Size: 617 1122 3345 2219 Fragments arranged by size: 3345 2219 1122 617 BsaAI yAC'GTr Cuts at: 493 1204 3791 5094 493 Size: 711 2587 1303 2702 Fragments arranged by size: 2702 2587 1303 711 BsaBI GATnn'nnATC Cuts at: 7297 7297 Size: 7303 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5110 5492 6127 275 Size: 53 83 186 936 3577 382 635 1451 Fragments arranged by size: 3577 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5736 6134 6408 6555 6966 5736 Size: 398 274 147 411 6073 Fragments arranged by size: 6073 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7078 Fragments arranged by size: 7078 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3917 1186 Size: 2731 4572 BseYI C'CCAG_C Cuts at: 6506 7002 6506 Size: 496 6807 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6730 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 4753 5514 6542 1540 Size: 275 186 12 2740 761 1028 2301 Fragments arranged by size: 2740 2301 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 3401 3811 5364 5449 6520 3401 Size: 410 1553 85 1071 4184 Fragments arranged by size: 4184 1553 1071 410 85 BsmI GAATG_Cn' Cuts at: 2041 4814 4907 2041 Size: 2773 93 4437 Fragments arranged by size: 4437 2773 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4151 4151 Size: 7303 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3401 3481 3811 4622 5364 5449 6520 Size: 399 1611 80 330 811 742 85 1071 Cuts at: 6520 1391 Size: 2174 Fragments arranged by size: 2174 1611 1071 811 742 399 330 85 80 BspEI T'CCGG_A Cuts at: 6966 6966 Size: 7303 BspHI T'CATG_A Cuts at: 3529 3709 4129 5192 6922 3529 Size: 180 420 1063 1730 3910 Fragments arranged by size: 3910 1730 1063 420 180 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2446 2603 4658 844 Size: 1602 157 2055 3489 Fragments arranged by size: 3489 2055 1602 157 BsrBI CCG'CTC Cuts at: 1394 1823 5190 1394 Size: 429 3367 3507 Fragments arranged by size: 3507 3367 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3501 4286 5798 5972 65 Size: 1183 2253 785 1512 174 1396 Fragments arranged by size: 2253 1512 1396 1183 785 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 3072 5947 6134 1700 Size: 110 198 1064 2875 187 2869 Fragments arranged by size: 2875 2869 1064 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7303 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7303 BssSI C'ACGA_G Cuts at: 3239 5363 6375 3239 Size: 2124 1012 4167 Fragments arranged by size: 4167 2124 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2967 3683 794 Size: 2173 716 4414 Fragments arranged by size: 4414 2173 716 BstBI TT'CG_AA Cuts at: 6107 6107 Size: 7303 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7303 BstXI CCAn_nnnn'nTGG Cuts at: 2875 2875 Size: 7303 BstYI r'GATC_y Cuts at: 1972 2052 2389 3693 4330 4633 5000 5385 5402 Size: 80 337 1304 637 303 367 385 17 Cuts at: 5402 6854 6940 6952 7298 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1304 637 385 367 346 337 303 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 2945 2945 Size: 7303 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6751 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6564 Fragments arranged by size: 6564 683 56 BtsI GCAGTG_nn' Cuts at: 3458 4167 4819 5613 5633 3458 Size: 709 652 794 20 5128 Fragments arranged by size: 5128 794 709 652 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7303 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7268 DraI TTT'AAA Cuts at: 2923 4607 2923 Size: 1684 5619 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6310 809 Size: 507 4994 1802 Fragments arranged by size: 4994 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 3945 4590 Size: 54 1425 50 275 18 180 1935 645 Cuts at: 4590 4750 5639 7268 8 Size: 160 889 1629 43 Fragments arranged by size: 1935 1629 1425 889 645 275 180 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 4750 1537 Size: 275 198 2740 4090 Fragments arranged by size: 4090 2740 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 2231 3027 3799 3828 4530 5233 1353 Size: 695 183 796 772 29 702 703 3423 Fragments arranged by size: 3423 796 772 703 702 695 183 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5890 6264 6410 387 Size: 1064 138 4301 374 146 1280 Fragments arranged by size: 4301 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3809 3809 Size: 7303 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2411 3408 4385 4602 5366 5963 Size: 393 623 156 997 977 217 764 597 Cuts at: 5963 6750 7018 1239 Size: 787 268 1524 Fragments arranged by size: 1524 997 977 787 764 623 597 393 268 217 156 EcoO109I rG'GnC_Cy Cuts at: 2359 2359 Size: 7303 EcoRI G'AATT_C Cuts at: 4276 4612 4990 4276 Size: 336 378 6589 Fragments arranged by size: 6589 378 336 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2071 2360 2717 3662 3944 2071 Size: 289 357 945 282 5430 Fragments arranged by size: 5430 945 357 289 282 FspI TGC'GCA Cuts at: 2442 5809 2442 Size: 3367 3936 HaeII r_GCGC'y Cuts at: 1678 6450 1678 Size: 4772 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2848 2880 3898 3930 4009 Size: 32 135 32 1276 32 1018 32 79 Cuts at: 4009 4041 5944 5976 6018 6050 1373 Size: 32 1903 32 42 32 2626 Fragments arranged by size: 2626 1903 1276 1018 135 79 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4647 4893 669 Size: 988 281 2709 246 3079 Fragments arranged by size: 3079 2709 988 281 246 HindIII A'AGCT_T Cuts at: 748 2196 2628 3704 4493 748 Size: 1448 432 1076 789 3558 Fragments arranged by size: 3558 1448 1076 789 432 HpaI GTT'AAC Cuts at: 4893 4893 Size: 7303 KpnI G_GTAC'C Cuts at: 4628 4628 Size: 7303 MfeI C'AATT_G Cuts at: 4902 4902 Size: 7303 MluI A'CGCG_T Cuts at: 7179 7179 Size: 7303 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6417 6601 1305 Size: 5112 184 2007 Fragments arranged by size: 5112 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3947 4592 10 Size: 54 1425 343 2115 645 2721 Fragments arranged by size: 2721 2115 1425 645 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2259 2326 5261 5620 5779 518 Size: 648 1093 67 2935 359 159 2042 Fragments arranged by size: 2935 2042 1093 648 359 159 67 MspA1I CmG'CkG Cuts at: 1606 3428 4307 4719 5396 6544 6789 1606 Size: 1822 879 412 677 1148 245 2120 Fragments arranged by size: 2120 1822 1148 879 677 412 245 NaeI GCC'GGC Cuts at: 2010 2010 Size: 7303 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6751 NdeI CA'TA_TG Cuts at: 387 3616 387 Size: 3229 4074 NgoMIV G'CCGG_C Cuts at: 2008 2008 Size: 7303 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7303 NotI GC'GGCC_GC Cuts at: 4750 4750 Size: 7303 NspI r_CATG'y Cuts at: 1132 1229 3266 3361 3460 3766 4410 4661 6206 Size: 97 2037 95 99 306 644 251 1545 Cuts at: 6206 1132 Size: 2229 Fragments arranged by size: 2229 2037 1545 644 306 251 99 97 95 PasI CC'CwG_GG Cuts at: 1348 2868 1348 Size: 1520 5783 PciI A'CATG_T Cuts at: 3762 4406 6202 3762 Size: 644 1796 4863 Fragments arranged by size: 4863 1796 644 PflMI CCAn_nnn'nTGG Cuts at: 1108 3313 1108 Size: 2205 5098 PfoI T'CCnGG_A Cuts at: 4525 4525 Size: 7303 PmeI GTTT'AAAC Cuts at: 4607 4607 Size: 7303 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3222 3254 5372 5404 6885 6917 3222 Size: 32 2118 32 1481 32 3608 Fragments arranged by size: 3608 2118 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2359 2359 Size: 7303 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7303 PsiI TTA'TAA Cuts at: 3489 4873 3489 Size: 1384 5919 PstI C_TGCA'G Cuts at: 830 2737 4162 4576 4655 830 Size: 1907 1425 414 79 3478 Fragments arranged by size: 3478 1907 1425 414 79 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7303 PvuII CAG'CTG Cuts at: 4307 4307 Size: 7303 SacI G_AGCT'C Cuts at: 3811 3811 Size: 7303 SalI G'TCGA_C Cuts at: 1936 4645 1936 Size: 2709 4594 SapI GCTCTTCn'nnn_ Cuts at: 2048 3799 2048 Size: 1751 5552 SbfI CC_TGCA'GG Cuts at: 4655 4655 Size: 7303 ScaI AGT'ACT Cuts at: 3137 5551 7263 3137 Size: 2414 1712 3177 Fragments arranged by size: 3177 2414 1712 SfcI C'TryA_G Cuts at: 826 1045 2372 2620 2679 2733 3687 4158 4353 Size: 219 1327 248 59 54 954 471 195 Cuts at: 4353 4572 4651 5786 6467 6658 826 Size: 219 79 1135 681 191 1471 Fragments arranged by size: 1471 1327 1135 954 681 471 248 219 219 195 191 79 59 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7303 SmaI CCC'GGG Cuts at: 1872 4630 1872 Size: 2758 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2056 2353 2393 2807 3518 5407 Size: 197 926 113 297 40 414 711 1889 Cuts at: 5407 6308 6570 820 Size: 901 262 1553 Fragments arranged by size: 1889 1553 926 901 711 414 297 262 197 113 40 SnaBI TAC'GTA Cuts at: 493 3791 5094 493 Size: 3298 1303 2702 Fragments arranged by size: 3298 2702 1303 SpeI A'CTAG_T Cuts at: 152 152 Size: 7303 SphI G_CATG'C Cuts at: 1132 3266 4661 1132 Size: 2134 1395 3774 Fragments arranged by size: 3774 2134 1395 SspI AAT'ATT Cuts at: 5 52 3274 5227 5 Size: 47 3222 1953 2081 Fragments arranged by size: 3222 2081 1953 47 StuI AGG'CCT Cuts at: 1517 3311 1517 Size: 1794 5509 StyI C'CwwG_G Cuts at: 513 1065 2028 2148 3312 3948 4444 4742 513 Size: 552 963 120 1164 636 496 298 3074 Fragments arranged by size: 3074 1164 963 636 552 496 298 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5330 5347 5500 5685 6130 325 Size: 1255 3750 17 153 185 445 1498 Fragments arranged by size: 3750 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3135 3189 3212 Size: 274 80 33 51 1443 1158 54 23 Cuts at: 3212 3643 5549 7261 96 Size: 431 1906 1712 138 Fragments arranged by size: 1906 1712 1443 1158 431 274 138 80 54 51 33 23 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7263 7295 7263 Size: 32 7271 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7303 XbaI T'CTAG_A Cuts at: 4639 4639 Size: 7303 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7303 XmaI C'CCGG_G Cuts at: 1870 4628 1870 Size: 2758 4545 XmnI GAAnn'nnTTC Cuts at: 2208 3234 3930 4065 4263 5432 2208 Size: 1026 696 135 198 1169 4079 Fragments arranged by size: 4079 1169 1026 696 198 135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5111 276 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI