(Circular) (Six-base) MAPSORT of: pFN21AB8532.seq Check: 5716 from: 1 to: 7399 pFN21AB8532 7399 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5209 278 Size: 53 83 186 936 3673 2468 Fragments arranged by size: 3673 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 4742 1937 Size: 517 2288 4594 Fragments arranged by size: 4594 2288 517 Acc65I G'GTAC_C Cuts at: 4720 4720 Size: 7399 AclI AA'CG_TT Cuts at: 1563 5526 5899 1563 Size: 3963 373 3063 Fragments arranged by size: 3963 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2693 3260 3438 5462 6846 7114 1632 Size: 1061 567 178 2024 1384 268 1917 Fragments arranged by size: 2024 1917 1384 1061 567 268 178 AfeI AGC'GCT Cuts at: 3677 3677 Size: 7399 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7202 AflIII A'CryG_T Cuts at: 1203 6298 7275 1203 Size: 5095 977 1327 Fragments arranged by size: 5095 1327 977 AgeI A'CCGG_T Cuts at: 4189 6230 4189 Size: 2041 5358 AhdI GACnn_n'nnGTC Cuts at: 6128 6128 Size: 7399 AleI CACnn'nnGTG Cuts at: 1166 2720 1166 Size: 1554 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2738 2873 1184 Size: 1554 135 5710 Fragments arranged by size: 5710 1554 135 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2093 2125 4577 4609 2093 Size: 32 2452 32 4883 Fragments arranged by size: 4883 2452 32 32 AlwNI CAG_nnn'CTG Cuts at: 5142 6714 5142 Size: 1572 5827 ApaI G_GGCC'C Cuts at: 3282 3348 3282 Size: 66 7333 ApaLI G'TGCA_C Cuts at: 2713 5456 6612 2713 Size: 2743 1156 3500 Fragments arranged by size: 3500 2743 1156 ApoI r'AATT_y Cuts at: 1493 2019 3854 4433 4708 4934 5086 7195 1493 Size: 526 1835 579 275 226 152 2109 1697 Fragments arranged by size: 2109 1835 1697 579 526 275 226 152 AseI AT'TA_AT Cuts at: 160 1032 5953 160 Size: 872 4921 1606 Fragments arranged by size: 4921 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7399 AvaI C'yCGr_G Cuts at: 1870 1943 2756 3430 4255 4394 4597 4724 1870 Size: 73 813 674 825 139 203 127 4545 Fragments arranged by size: 4545 825 813 674 203 139 127 73 AvrII C'CTAG_G Cuts at: 3004 3004 Size: 7399 BamHI G'GATC_C Cuts at: 4409 4729 5096 4409 Size: 320 367 6712 Fragments arranged by size: 6712 367 320 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3992 4192 4720 6175 618 Size: 325 217 627 2205 200 528 1455 1842 Fragments arranged by size: 2205 1842 1455 627 528 325 217 200 BanII G_rGCy'C Cuts at: 1391 2763 3201 3282 3348 3633 3645 3829 4304 Size: 1372 438 81 66 285 12 184 475 Cuts at: 4304 4718 1391 Size: 414 4072 Fragments arranged by size: 4072 1372 475 438 414 285 184 81 66 12 BbeI G_GCGC'C Cuts at: 3996 3996 Size: 7399 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2252 2433 928 Size: 971 353 181 5894 Fragments arranged by size: 5894 971 353 181 BbvCI CC'TCA_GC Cuts at: 3453 3453 Size: 7399 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2444 2478 3313 3347 5590 Size: 34 50 34 423 34 835 34 2243 Cuts at: 5590 5624 1903 Size: 34 3678 Fragments arranged by size: 3678 2243 835 423 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5291 6501 5291 Size: 1210 6189 BclI T'GATC_A Cuts at: 1548 1548 Size: 7399 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4464 6010 136 Size: 107 122 71 631 605 2792 1546 1525 Fragments arranged by size: 2792 1546 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7394 7394 Size: 7399 BlpI GC'TnA_GC Cuts at: 3864 4816 3864 Size: 952 6447 Bme1580I G_kGCm'C Cuts at: 1790 2208 2717 2727 3282 3348 3918 4197 5460 Size: 418 509 10 555 66 570 279 1263 Cuts at: 5460 6616 1790 Size: 1156 2573 Fragments arranged by size: 2573 1263 1156 570 555 509 418 279 66 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7399 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3053 3285 3488 Size: 416 53 83 399 24 1636 232 203 Cuts at: 3488 3593 3824 5130 6088 442 Size: 105 231 1306 958 1753 Fragments arranged by size: 1753 1636 1306 958 416 399 232 231 203 105 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7399 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3664 3664 Size: 7399 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2354 2580 2852 6059 1239 Size: 869 246 226 272 3207 2579 Fragments arranged by size: 3207 2579 869 272 246 226 Bpu10I CC'TnA_GC Cuts at: 3453 4483 3453 Size: 1030 6369 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2300 2722 3617 5524 6389 6687 2300 Size: 422 895 1907 865 298 3012 Fragments arranged by size: 3012 1907 895 865 422 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3452 3647 6062 882 Size: 617 1953 195 2415 2219 Fragments arranged by size: 2415 2219 1953 617 195 BsaAI yAC'GTr Cuts at: 493 1204 3373 5190 493 Size: 711 2169 1817 2702 Fragments arranged by size: 2702 2169 1817 711 BsaBI GATnn'nnATC Cuts at: 7393 7393 Size: 7399 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2627 2843 3993 5206 Size: 53 83 186 936 1094 216 1150 1213 Cuts at: 5206 5588 6223 275 Size: 382 635 1451 Fragments arranged by size: 1451 1213 1150 1094 936 635 382 216 186 83 53 BsaWI w'CCGG_w Cuts at: 4189 5832 6230 6504 6651 7062 4189 Size: 1643 398 274 147 411 4526 Fragments arranged by size: 4526 1643 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3131 3161 4364 4394 4577 Size: 30 165 30 1728 30 1203 30 183 Cuts at: 4577 4607 1178 Size: 30 3970 Fragments arranged by size: 3970 1728 1203 183 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 3143 3155 3200 3470 4135 4537 1186 Size: 910 1047 12 45 270 665 402 4048 Fragments arranged by size: 4048 1047 910 665 402 270 45 12 BseYI C'CCAG_C Cuts at: 2234 3152 4025 4208 4628 6602 7098 2234 Size: 918 873 183 420 1974 496 2535 Fragments arranged by size: 2535 1974 918 873 496 420 183 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3353 1163 Size: 573 1617 5209 Fragments arranged by size: 5209 1617 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4849 5610 6638 1540 Size: 275 186 2848 761 1028 2301 Fragments arranged by size: 2848 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 2717 2763 3645 5460 5545 6616 2256 Size: 461 46 882 1815 85 1071 3039 Fragments arranged by size: 3039 1815 1071 882 461 85 46 BsmI GAATG_Cn' Cuts at: 2056 3220 4021 4099 4114 4910 5003 2056 Size: 1164 801 78 15 796 93 4452 Fragments arranged by size: 4452 1164 801 796 93 78 15 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2256 2717 2727 2763 3201 3282 Size: 399 418 48 461 10 36 438 81 Cuts at: 3282 3348 3633 3645 3829 3918 4197 4304 4718 Size: 66 285 12 184 89 279 107 414 Cuts at: 4718 5460 5545 6616 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 461 438 418 414 399 285 279 184 107 89 85 81 66 48 36 12 10 BspEI T'CCGG_A Cuts at: 7062 7062 Size: 7399 BspHI T'CATG_A Cuts at: 5288 7018 5288 Size: 1730 5669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4754 844 Size: 3910 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2122 5286 1394 Size: 429 299 3164 3507 Fragments arranged by size: 3507 3164 429 299 BsrDI GCAATG_nn' Cuts at: 65 1248 5894 6068 65 Size: 1183 4646 174 1396 Fragments arranged by size: 4646 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4019 4189 6043 6230 1700 Size: 110 2209 170 1854 187 2869 Fragments arranged by size: 2869 2209 1854 187 170 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7399 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7399 BssSI C'ACGA_G Cuts at: 5459 6471 5459 Size: 1012 6387 BstAPI GCAn_nnn'nTGC Cuts at: 794 2968 794 Size: 2174 5225 BstBI TT'CG_AA Cuts at: 6203 6203 Size: 7399 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7399 BstXI CCAn_nnnn'nTGG Cuts at: 3310 3722 3310 Size: 412 6987 BstYI r'GATC_y Cuts at: 1972 2329 3078 4409 4729 5096 5481 5498 6950 Size: 357 749 1331 320 367 385 17 1452 Cuts at: 6950 7036 7048 7394 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1331 749 385 367 357 346 320 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4000 4351 513 Size: 552 2935 351 3561 Fragments arranged by size: 3561 2935 552 351 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6660 Fragments arranged by size: 6660 683 56 BtsI GCAGTG_nn' Cuts at: 2966 3483 3543 4915 5709 5729 2966 Size: 517 60 1372 794 20 4636 Fragments arranged by size: 4636 1372 794 517 60 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7399 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4426 4461 529 Size: 35 3862 35 3467 Fragments arranged by size: 3862 3467 35 35 DraI TTT'AAA Cuts at: 4703 4703 Size: 7399 DraIII CAC_nnn'GTG Cuts at: 2954 2954 Size: 7399 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6406 809 Size: 507 5090 1802 Fragments arranged by size: 5090 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2388 2400 2787 Size: 54 1425 50 275 18 558 12 387 Cuts at: 2787 2986 3360 3375 4606 4846 5735 7364 8 Size: 199 374 15 1231 240 889 1629 43 Fragments arranged by size: 1629 1425 1231 889 558 387 374 275 240 199 54 50 43 18 15 12 EagI C'GGCC_G Cuts at: 1537 1812 4846 1537 Size: 275 3034 4090 Fragments arranged by size: 4090 3034 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3614 3815 3940 5329 1353 Size: 2261 201 125 1389 3423 Fragments arranged by size: 3423 2261 1389 201 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4422 4542 5986 6360 6506 387 Size: 1064 138 2833 120 1444 374 146 1280 Fragments arranged by size: 2833 1444 1280 1064 374 146 138 120 EcoICRI GAG'CTC Cuts at: 2761 3643 2761 Size: 882 6517 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2354 2580 2693 2852 3260 3438 Size: 393 476 246 226 113 159 408 178 Cuts at: 3438 5462 6059 6846 7114 1239 Size: 2024 597 787 268 1524 Fragments arranged by size: 2024 1524 787 597 476 408 393 268 246 226 178 159 113 EcoNI CCTnn'n_nnAGG Cuts at: 2315 2381 2315 Size: 66 7333 EcoO109I rG'GnC_Cy Cuts at: 2305 2707 2735 2815 3345 3896 2305 Size: 402 28 80 530 551 5808 Fragments arranged by size: 5808 551 530 402 80 28 EcoRI G'AATT_C Cuts at: 4708 5086 4708 Size: 378 7021 EcoRV GAT'ATC Cuts at: 2944 2944 Size: 7399 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2341 2341 Size: 7399 FspI TGC'GCA Cuts at: 2958 5905 2958 Size: 2947 4452 HaeII r_GCGC'y Cuts at: 1678 2129 3679 3996 6546 1678 Size: 451 1550 317 2550 2531 Fragments arranged by size: 2550 2531 1550 451 317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3131 3163 6040 6072 6114 Size: 32 135 32 1559 32 2877 32 42 Cuts at: 6114 6146 1373 Size: 32 2626 Fragments arranged by size: 2877 2626 1559 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2455 2578 4743 4989 669 Size: 988 281 517 123 2165 246 3079 Fragments arranged by size: 3079 2165 988 517 281 246 123 HindIII A'AGCT_T Cuts at: 748 3776 748 Size: 3028 4371 HpaI GTT'AAC Cuts at: 4989 4989 Size: 7399 KasI G'GCGC_C Cuts at: 3992 3992 Size: 7399 KpnI G_GTAC'C Cuts at: 4724 4724 Size: 7399 MfeI C'AATT_G Cuts at: 4998 4998 Size: 7399 MluI A'CGCG_T Cuts at: 7275 7275 Size: 7399 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2096 2166 2659 3753 4437 6513 6697 1305 Size: 791 70 493 1094 684 2076 184 2007 Fragments arranged by size: 2076 2007 1094 791 684 493 184 70 MscI TGG'CCA Cuts at: 10 64 1489 1832 2402 10 Size: 54 1425 343 570 5007 Fragments arranged by size: 5007 1425 570 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2720 2855 3308 5357 5716 5875 Size: 648 1035 519 135 453 2049 359 159 Cuts at: 5875 518 Size: 2042 Fragments arranged by size: 2049 2042 1035 648 519 453 359 159 135 MspA1I CmG'CkG Cuts at: 1606 3238 4815 5492 6640 6885 1606 Size: 1632 1577 677 1148 245 2120 Fragments arranged by size: 2120 1632 1577 1148 677 245 NarI GG'CG_CC Cuts at: 3993 3993 Size: 7399 NcoI C'CATG_G Cuts at: 513 1065 4000 4351 513 Size: 552 2935 351 3561 Fragments arranged by size: 3561 2935 552 351 NdeI CA'TA_TG Cuts at: 387 387 Size: 7399 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7399 NotI GC'GGCC_GC Cuts at: 4846 4846 Size: 7399 NspI r_CATG'y Cuts at: 1132 1229 4757 6302 1132 Size: 97 3528 1545 2229 Fragments arranged by size: 3528 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4505 1348 Size: 3157 4242 PciI A'CATG_T Cuts at: 6298 6298 Size: 7399 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 2104 1108 Size: 939 57 6403 Fragments arranged by size: 6403 939 57 PfoI T'CCnGG_A Cuts at: 2376 2376 Size: 7399 PmeI GTTT'AAAC Cuts at: 4703 4703 Size: 7399 PmlI CAC'GTG Cuts at: 3373 3373 Size: 7399 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4577 4609 5468 5500 6981 7013 4577 Size: 32 859 32 1481 32 4963 Fragments arranged by size: 4963 1481 859 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2707 2735 2707 Size: 28 7371 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7399 PsiI TTA'TAA Cuts at: 4969 4969 Size: 7399 PspOMI G'GGCC_C Cuts at: 3278 3344 3278 Size: 66 7333 PspXI vC'TCGA_Gb Cuts at: 3430 3430 Size: 7399 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4676 4708 4676 Size: 32 7367 PstI C_TGCA'G Cuts at: 830 3255 4751 830 Size: 2425 1496 3478 Fragments arranged by size: 3478 2425 1496 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7399 PvuII CAG'CTG Cuts at: 3238 3238 Size: 7399 SacI G_AGCT'C Cuts at: 2763 3645 2763 Size: 882 6517 SalI G'TCGA_C Cuts at: 1936 2453 4741 1936 Size: 517 2288 4594 Fragments arranged by size: 4594 2288 517 SanDI GG'GwC_CC Cuts at: 2735 2735 Size: 7399 SbfI CC_TGCA'GG Cuts at: 4751 4751 Size: 7399 ScaI AGT'ACT Cuts at: 5647 7359 5647 Size: 1712 5687 SfcI C'TryA_G Cuts at: 826 1045 3251 4072 4084 4306 4441 4615 4747 Size: 219 2206 821 12 222 135 174 132 Cuts at: 4747 5882 6563 6754 826 Size: 1135 681 191 1471 Fragments arranged by size: 2206 1471 1135 821 681 222 219 191 174 135 132 12 SfoI GGC'GCC Cuts at: 3994 3994 Size: 7399 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7399 SgrAI Cr'CCGG_yG Cuts at: 1700 4189 1700 Size: 2489 4910 SmaI CCC'GGG Cuts at: 1872 4396 4599 4726 1872 Size: 2524 203 127 4545 Fragments arranged by size: 4545 2524 203 127 SmlI C'TyrA_G Cuts at: 820 1017 1943 2315 2701 2756 3430 3632 5503 Size: 197 926 372 386 55 674 202 1871 Cuts at: 5503 6404 6666 820 Size: 901 262 1553 Fragments arranged by size: 1871 1553 926 901 674 386 372 262 202 197 55 SnaBI TAC'GTA Cuts at: 493 5190 493 Size: 4697 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7399 SphI G_CATG'C Cuts at: 1132 4757 1132 Size: 3625 3774 SspI AAT'ATT Cuts at: 5 52 5323 5 Size: 47 5271 2081 Fragments arranged by size: 5271 2081 47 StuI AGG'CCT Cuts at: 1517 3338 3749 1517 Size: 1821 411 5167 Fragments arranged by size: 5167 1821 411 StyI C'CwwG_G Cuts at: 513 1065 2622 3004 3715 4000 4168 4351 4555 Size: 552 1557 382 711 285 168 183 204 Cuts at: 4555 4838 513 Size: 283 3074 Fragments arranged by size: 3074 1557 711 552 382 285 283 204 183 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2792 2812 3476 3865 5426 5443 5596 Size: 1255 1212 20 664 389 1561 17 153 Cuts at: 5596 5781 6226 325 Size: 185 445 1498 Fragments arranged by size: 1561 1498 1255 1212 664 445 389 185 153 20 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3255 4692 5645 Size: 274 80 33 51 1443 1278 1437 953 Cuts at: 5645 7357 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1437 1278 953 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2504 2536 2719 2751 4099 4131 7359 7391 2504 Size: 32 183 32 1348 32 3228 32 2512 Fragments arranged by size: 3228 2512 1348 183 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7399 XbaI T'CTAG_A Cuts at: 2920 4735 2920 Size: 1815 5584 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2797 3370 3736 4616 2797 Size: 573 366 880 5580 Fragments arranged by size: 5580 880 573 366 XhoI C'TCGA_G Cuts at: 1943 2756 3430 1943 Size: 813 674 5912 Fragments arranged by size: 5912 813 674 XmaI C'CCGG_G Cuts at: 1870 4394 4597 4724 1870 Size: 2524 203 127 4545 Fragments arranged by size: 4545 2524 203 127 XmnI GAAnn'nnTTC Cuts at: 5528 5528 Size: 7399 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5207 276 Size: 53 83 186 936 3673 2468 Fragments arranged by size: 3673 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I FseI FspAI NaeI NgoMIV NruI NsiI PacI RsrII SacII SapI SexAI SfiI SrfI SwaI