(Circular) (Six-base) MAPSORT of: pFN21AB8475.seq Check: 4329 from: 1 to: 7354 pFN21AB8475 7354 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3055 5164 278 Size: 53 83 186 936 1519 2109 2468 Fragments arranged by size: 2468 2109 1519 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4276 4697 1937 Size: 2339 421 4594 Fragments arranged by size: 4594 2339 421 Acc65I G'GTAC_C Cuts at: 4675 4675 Size: 7354 AclI AA'CG_TT Cuts at: 1563 5481 5854 1563 Size: 3918 373 3063 Fragments arranged by size: 3918 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3824 5417 6801 7069 1632 Size: 2192 1593 1384 268 1917 Fragments arranged by size: 2192 1917 1593 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3740 4091 820 Size: 197 2723 351 4083 Fragments arranged by size: 4083 2723 351 197 AflIII A'CryG_T Cuts at: 1203 3096 4279 6253 7230 1203 Size: 1893 1183 1974 977 1327 Fragments arranged by size: 1974 1893 1327 1183 977 AgeI A'CCGG_T Cuts at: 6185 6185 Size: 7354 AhdI GACnn_n'nnGTC Cuts at: 6083 6083 Size: 7354 AleI CACnn'nnGTG Cuts at: 1166 2517 3966 1166 Size: 1351 1449 4554 Fragments arranged by size: 4554 1449 1351 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7354 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3675 3707 3675 Size: 32 7322 AlwNI CAG_nnn'CTG Cuts at: 4310 5097 6669 4310 Size: 787 1572 4995 Fragments arranged by size: 4995 1572 787 ApaLI G'TGCA_C Cuts at: 5411 6567 5411 Size: 1156 6198 ApoI r'AATT_y Cuts at: 1493 2255 2279 2588 2705 3861 4663 4889 5041 Size: 762 24 309 117 1156 802 226 152 Cuts at: 5041 7150 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1156 802 762 309 226 152 117 24 AseI AT'TA_AT Cuts at: 160 1032 3825 5908 160 Size: 872 2793 2083 1606 Fragments arranged by size: 2793 2083 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7354 AvaI C'yCGr_G Cuts at: 1870 1943 2946 4679 1870 Size: 73 1003 1733 4545 Fragments arranged by size: 4545 1733 1003 73 BamHI G'GATC_C Cuts at: 4684 5051 4684 Size: 367 6987 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4675 6130 618 Size: 325 217 627 2888 1455 1842 Fragments arranged by size: 2888 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2997 4462 4474 4673 1391 Size: 1606 1465 12 199 4072 Fragments arranged by size: 4072 1606 1465 199 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3219 3477 928 Size: 971 1320 258 4805 Fragments arranged by size: 4805 1320 971 258 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5545 5579 1903 Size: 34 3608 34 3678 Fragments arranged by size: 3678 3608 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4580 5246 6456 4580 Size: 666 1210 5478 Fragments arranged by size: 5478 1210 666 BclI T'GATC_A Cuts at: 1548 1548 Size: 7354 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5965 136 Size: 107 122 71 631 605 4293 1525 Fragments arranged by size: 4293 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2869 4634 7349 2869 Size: 1765 2715 2874 Fragments arranged by size: 2874 2715 1765 BlpI GC'TnA_GC Cuts at: 3075 3132 4771 3075 Size: 57 1639 5658 Fragments arranged by size: 5658 1639 57 Bme1580I G_kGCm'C Cuts at: 1790 5415 6571 1790 Size: 3625 1156 2573 Fragments arranged by size: 3625 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3097 1318 Size: 1779 5575 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2046 3708 5085 Size: 416 53 83 399 24 629 1662 1377 Cuts at: 5085 6043 442 Size: 958 1753 Fragments arranged by size: 1753 1662 1377 958 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7354 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2973 3228 6014 1239 Size: 1734 255 2786 2579 Fragments arranged by size: 2786 2579 1734 255 Bpu10I CC'TnA_GC Cuts at: 2991 2991 Size: 7354 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2981 3620 5479 6344 6642 2981 Size: 639 1859 865 298 3693 Fragments arranged by size: 3693 1859 865 639 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6017 882 Size: 617 4518 2219 Fragments arranged by size: 4518 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5145 493 Size: 711 3941 2702 Fragments arranged by size: 3941 2702 711 BsaBI GATnn'nnATC Cuts at: 3762 7348 3762 Size: 3586 3768 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3052 5161 5543 6178 Size: 53 83 186 936 1519 2109 382 635 Cuts at: 6178 275 Size: 1451 Fragments arranged by size: 2109 1519 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2275 5787 6185 6459 6606 7017 2275 Size: 3512 398 274 147 411 2612 Fragments arranged by size: 3512 2612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3538 3568 3969 3999 1178 Size: 30 165 30 2135 30 401 30 4533 Fragments arranged by size: 4533 2135 401 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3413 3990 1186 Size: 2227 577 4550 Fragments arranged by size: 4550 2227 577 BseYI C'CCAG_C Cuts at: 3895 4081 6557 7053 3895 Size: 186 2476 496 4196 Fragments arranged by size: 4196 2476 496 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6781 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4804 5565 6593 1540 Size: 275 186 2803 761 1028 2301 Fragments arranged by size: 2803 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2997 3963 4462 5415 5500 6571 2997 Size: 966 499 953 85 1071 3780 Fragments arranged by size: 3780 1071 966 953 499 85 BsmI GAATG_Cn' Cuts at: 2931 3136 4430 4865 4958 2931 Size: 205 1294 435 93 5327 Fragments arranged by size: 5327 1294 435 205 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2997 3963 4462 4474 4673 5415 5500 Size: 399 1207 966 499 12 199 742 85 Cuts at: 5500 6571 1391 Size: 1071 2174 Fragments arranged by size: 2174 1207 1071 966 742 499 399 199 85 12 BspEI T'CCGG_A Cuts at: 2275 7017 2275 Size: 4742 2612 BspHI T'CATG_A Cuts at: 5243 6973 5243 Size: 1730 5624 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4556 4709 844 Size: 3712 153 3489 Fragments arranged by size: 3712 3489 153 BsrBI CCG'CTC Cuts at: 1394 1823 5241 1394 Size: 429 3418 3507 Fragments arranged by size: 3507 3418 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4525 5849 6023 65 Size: 1183 3277 1324 174 1396 Fragments arranged by size: 3277 1396 1324 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5998 6185 1700 Size: 110 4188 187 2869 Fragments arranged by size: 4188 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2429 2573 3442 96 Size: 2333 144 869 4008 Fragments arranged by size: 4008 2333 869 144 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7354 BssSI C'ACGA_G Cuts at: 5414 6426 5414 Size: 1012 6342 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7354 BstBI TT'CG_AA Cuts at: 2530 4465 6158 2530 Size: 1935 1693 3726 Fragments arranged by size: 3726 1935 1693 BstEII G'GTnAC_C Cuts at: 1184 3708 1184 Size: 2524 4830 BstYI r'GATC_y Cuts at: 1972 2114 2869 4634 4684 5051 5436 5453 6905 Size: 142 755 1765 50 367 385 17 1452 Cuts at: 6905 6991 7003 7349 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1765 1452 755 385 367 346 142 86 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 4486 513 Size: 552 939 2482 3381 Fragments arranged by size: 3381 2482 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6615 Fragments arranged by size: 6615 683 56 BtsI GCAGTG_nn' Cuts at: 4870 5664 5684 4870 Size: 794 20 6540 Fragments arranged by size: 6540 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7354 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7319 DraI TTT'AAA Cuts at: 2710 3060 3732 4658 2710 Size: 350 672 926 5406 Fragments arranged by size: 5406 926 672 350 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3097 6361 809 Size: 507 1781 3264 1802 Fragments arranged by size: 3264 1802 1781 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4066 4801 5690 Size: 54 1425 50 275 18 2236 735 889 Cuts at: 5690 7319 8 Size: 1629 43 Fragments arranged by size: 2236 1629 1425 889 735 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4801 1537 Size: 275 2989 4090 Fragments arranged by size: 4090 2989 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2065 2130 2757 2847 3354 3998 4004 4467 Size: 712 65 627 90 507 644 6 463 Cuts at: 4467 5284 1353 Size: 817 3423 Fragments arranged by size: 3423 817 712 644 627 507 463 90 65 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2674 5941 6315 6461 387 Size: 1064 138 1085 3267 374 146 1280 Fragments arranged by size: 3267 1280 1085 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2995 4460 2995 Size: 1465 5889 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2973 3228 3824 5417 6014 6801 7069 Size: 393 1341 255 596 1593 597 787 268 Cuts at: 7069 1239 Size: 1524 Fragments arranged by size: 1593 1524 1341 787 597 596 393 268 255 EcoNI CCTnn'n_nnAGG Cuts at: 2336 2336 Size: 7354 EcoRI G'AATT_C Cuts at: 4663 5041 4663 Size: 378 6976 EcoRV GAT'ATC Cuts at: 3166 3433 3166 Size: 267 7087 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2119 2213 3086 4527 2119 Size: 94 873 1441 4946 Fragments arranged by size: 4946 1441 873 94 FspI TGC'GCA Cuts at: 5860 5860 Size: 7354 HaeII r_GCGC'y Cuts at: 1678 3124 6501 1678 Size: 1446 3377 2531 Fragments arranged by size: 3377 2531 1446 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4258 4290 4325 4357 5995 Size: 32 135 32 2686 32 35 32 1638 Cuts at: 5995 6027 6069 6101 1373 Size: 32 42 32 2626 Fragments arranged by size: 2686 2626 1638 135 42 35 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4277 4698 4944 669 Size: 988 281 2339 421 246 3079 Fragments arranged by size: 3079 2339 988 421 281 246 HindIII A'AGCT_T Cuts at: 748 2646 3922 748 Size: 1898 1276 4180 Fragments arranged by size: 4180 1898 1276 HpaI GTT'AAC Cuts at: 4944 4944 Size: 7354 KpnI G_GTAC'C Cuts at: 4679 4679 Size: 7354 MfeI C'AATT_G Cuts at: 4953 4953 Size: 7354 MluI A'CGCG_T Cuts at: 7230 7230 Size: 7354 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3195 3718 6468 6652 1305 Size: 1890 523 2750 184 2007 Fragments arranged by size: 2750 2007 1890 523 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4068 10 Size: 54 1425 343 2236 3296 Fragments arranged by size: 3296 2236 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2410 2517 2936 3825 3966 5312 Size: 648 1056 188 107 419 889 141 1346 Cuts at: 5312 5671 5830 518 Size: 359 159 2042 Fragments arranged by size: 2042 1346 1056 889 648 419 359 188 159 141 107 MspA1I CmG'CkG Cuts at: 1606 3131 3755 3934 4770 5447 6595 6840 1606 Size: 1525 624 179 836 677 1148 245 2120 Fragments arranged by size: 2120 1525 1148 836 677 624 245 179 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5863 Fragments arranged by size: 5863 939 552 NdeI CA'TA_TG Cuts at: 387 2125 387 Size: 1738 5616 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7354 NotI GC'GGCC_GC Cuts at: 4801 4801 Size: 7354 NspI r_CATG'y Cuts at: 1132 1229 4283 4532 4712 6257 1132 Size: 97 3054 249 180 1545 2229 Fragments arranged by size: 3054 2229 1545 249 180 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7354 PciI A'CATG_T Cuts at: 4279 6253 4279 Size: 1974 5380 PflMI CCAn_nnn'nTGG Cuts at: 1108 2515 3662 1108 Size: 1407 1147 4800 Fragments arranged by size: 4800 1407 1147 PmeI GTTT'AAAC Cuts at: 4658 4658 Size: 7354 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5423 5455 6936 6968 5423 Size: 32 1481 32 5809 Fragments arranged by size: 5809 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2504 1613 Size: 891 6463 PsiI TTA'TAA Cuts at: 2201 2643 4924 2201 Size: 442 2281 4631 Fragments arranged by size: 4631 2281 442 PstI C_TGCA'G Cuts at: 830 4706 830 Size: 3876 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7354 PvuII CAG'CTG Cuts at: 3131 3755 3131 Size: 624 6730 SacI G_AGCT'C Cuts at: 2997 4462 2997 Size: 1465 5889 SalI G'TCGA_C Cuts at: 1936 4275 4696 1936 Size: 2339 421 4594 Fragments arranged by size: 4594 2339 421 SapI GCTCTTCn'nnn_ Cuts at: 4467 4467 Size: 7354 SbfI CC_TGCA'GG Cuts at: 4706 4706 Size: 7354 ScaI AGT'ACT Cuts at: 2451 5602 7314 2451 Size: 3151 1712 2491 Fragments arranged by size: 3151 2491 1712 SfcI C'TryA_G Cuts at: 826 1045 4702 5837 6518 6709 826 Size: 219 3657 1135 681 191 1471 Fragments arranged by size: 3657 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7354 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7354 SmaI CCC'GGG Cuts at: 1872 4681 1872 Size: 2809 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2996 3635 3740 4091 5458 6359 Size: 197 926 1053 639 105 351 1367 901 Cuts at: 6359 6621 820 Size: 262 1553 Fragments arranged by size: 1553 1367 1053 926 901 639 351 262 197 105 SnaBI TAC'GTA Cuts at: 493 5145 493 Size: 4652 2702 SpeI A'CTAG_T Cuts at: 152 2485 152 Size: 2333 5021 SphI G_CATG'C Cuts at: 1132 4712 1132 Size: 3580 3774 SspI AAT'ATT Cuts at: 5 52 2810 3527 5278 5 Size: 47 2758 717 1751 2081 Fragments arranged by size: 2758 2081 1751 717 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7354 StyI C'CwwG_G Cuts at: 513 1065 2004 2094 4793 513 Size: 552 939 90 2699 3074 Fragments arranged by size: 3074 2699 939 552 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2507 5381 5398 5551 5736 6181 325 Size: 1255 927 2874 17 153 185 445 1498 Fragments arranged by size: 2874 1498 1255 927 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2429 2449 2573 Size: 274 80 33 51 1443 452 20 124 Cuts at: 2573 3218 3442 3700 3928 4373 5600 7312 96 Size: 645 224 258 228 445 1227 1712 138 Fragments arranged by size: 1712 1443 1227 645 452 445 274 258 228 224 138 124 80 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3232 3264 7314 7346 3232 Size: 32 4050 32 3240 Fragments arranged by size: 4050 3240 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7354 XbaI T'CTAG_A Cuts at: 4690 4690 Size: 7354 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2516 4254 2516 Size: 1738 5616 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7354 XmaI C'CCGG_G Cuts at: 1870 4679 1870 Size: 2809 4545 XmnI GAAnn'nnTTC Cuts at: 4510 5483 4510 Size: 973 6381 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3053 5162 276 Size: 53 83 186 936 1519 2109 2468 Fragments arranged by size: 2468 2109 1519 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI