(Circular) (Six-base) MAPSORT of: pFN21AB6688.seq Check: 4618 from: 1 to: 6079 pFN21AB6688 6079 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2890 3889 278 Size: 53 83 186 936 1354 999 2468 Fragments arranged by size: 2468 1354 999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 3422 1937 Size: 1170 315 4594 Fragments arranged by size: 4594 1170 315 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2816 4142 5526 5794 1632 Size: 1184 1326 1384 268 1917 Fragments arranged by size: 1917 1384 1326 1184 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 4978 5955 1203 Size: 3775 977 1327 Fragments arranged by size: 3775 1327 977 AgeI A'CCGG_T Cuts at: 3268 4910 3268 Size: 1642 4437 AhdI GACnn_n'nnGTC Cuts at: 2318 4808 2318 Size: 2490 3589 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AlwNI CAG_nnn'CTG Cuts at: 3822 5394 3822 Size: 1572 4507 ApaLI G'TGCA_C Cuts at: 2042 4136 5292 2042 Size: 2094 1156 2829 Fragments arranged by size: 2829 2094 1156 ApoI r'AATT_y Cuts at: 1493 2144 2641 3123 3322 3388 3614 3766 5875 Size: 651 497 482 199 66 226 152 2109 Cuts at: 5875 1493 Size: 1697 Fragments arranged by size: 2109 1697 651 497 482 226 199 152 66 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 3404 1870 Size: 73 1461 4545 Fragments arranged by size: 4545 1461 73 AvrII C'CTAG_G Cuts at: 2891 2891 Size: 6079 BamHI G'GATC_C Cuts at: 3409 3776 3409 Size: 367 5712 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2897 2976 3400 4855 618 Size: 325 217 627 1110 79 424 1455 1842 Fragments arranged by size: 1842 1455 1110 627 424 325 217 79 BanII G_rGCy'C Cuts at: 1391 2090 3300 3398 1391 Size: 699 1210 98 4072 Fragments arranged by size: 4072 1210 699 98 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2608 2642 4270 4304 1903 Size: 34 671 34 1628 34 3678 Fragments arranged by size: 3678 1628 671 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 2226 2967 1548 Size: 678 741 4660 Fragments arranged by size: 4660 741 678 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4690 136 Size: 107 122 71 631 605 3018 1525 Fragments arranged by size: 3018 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 3496 3496 Size: 6079 Bme1580I G_kGCm'C Cuts at: 1790 2046 2387 2900 2981 4140 5296 1790 Size: 256 341 513 81 1159 1156 2573 Fragments arranged by size: 2573 1159 1156 513 341 256 81 BmgBI CAC'GTC Cuts at: 1318 2622 1318 Size: 1304 4775 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2084 3810 4768 Size: 416 53 83 399 24 667 1726 958 Cuts at: 4768 442 Size: 1753 Fragments arranged by size: 1753 1726 958 667 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3331 4739 1239 Size: 2092 1408 2579 Fragments arranged by size: 2579 2092 1408 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4204 5069 5367 4204 Size: 865 298 4916 Fragments arranged by size: 4916 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4742 882 Size: 617 3243 2219 Fragments arranged by size: 3243 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2474 2703 3870 493 Size: 711 1270 229 1167 2702 Fragments arranged by size: 2702 1270 1167 711 229 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2887 3886 4268 4903 Size: 53 83 186 936 1354 999 382 635 Cuts at: 4903 275 Size: 1451 Fragments arranged by size: 1451 1354 999 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3268 4512 4910 5184 5331 5742 3268 Size: 1244 398 274 147 411 3605 Fragments arranged by size: 3605 1244 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5854 Fragments arranged by size: 5854 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6079 BseYI C'CCAG_C Cuts at: 5282 5778 5282 Size: 496 5583 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2240 2735 1163 Size: 573 504 495 4507 Fragments arranged by size: 4507 573 504 495 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2820 3529 4290 5318 1540 Size: 275 186 819 709 761 1028 2301 Fragments arranged by size: 2301 1028 819 761 709 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2631 4140 4225 5296 2046 Size: 585 1509 85 1071 2829 Fragments arranged by size: 2829 1509 1071 585 85 BsmI GAATG_Cn' Cuts at: 2026 2029 3137 3590 3683 2026 Size: 3 1108 453 93 4422 Fragments arranged by size: 4422 1108 453 93 3 BsmBI CGTCTCn'nnnn_ Cuts at: 2878 2878 Size: 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2090 2387 2631 2900 2981 3300 Size: 399 256 44 297 244 269 81 319 Cuts at: 3300 3398 4140 4225 5296 1391 Size: 98 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 319 297 269 256 244 98 85 81 44 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 2199 2520 3968 5698 2199 Size: 321 1448 1730 2580 Fragments arranged by size: 2580 1730 1448 321 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3434 844 Size: 2590 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2292 4574 4748 65 Size: 1183 1044 2282 174 1396 Fragments arranged by size: 2282 1396 1183 1044 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3268 4723 4910 1700 Size: 110 1458 1455 187 2869 Fragments arranged by size: 2869 1458 1455 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6079 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 2933 4883 2933 Size: 1950 4129 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstYI r'GATC_y Cuts at: 1972 3409 3776 4161 4178 5630 5716 5728 6074 Size: 1437 367 385 17 1452 86 12 346 Cuts at: 6074 1972 Size: 1977 Fragments arranged by size: 1977 1452 1437 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3108 3108 Size: 6079 Bsu36I CC'TnA_GG Cuts at: 2015 2015 Size: 6079 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 3213 3379 3595 4389 4409 3213 Size: 166 216 794 20 4883 Fragments arranged by size: 4883 794 216 166 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DraIII CAC_nnn'GTG Cuts at: 2622 2622 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2168 2363 2755 Size: 54 1425 50 275 18 338 195 392 Cuts at: 2755 2817 3526 4415 6044 8 Size: 62 709 889 1629 43 Fragments arranged by size: 1629 1425 889 709 392 338 275 195 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2817 3526 1537 Size: 275 1005 709 4090 Fragments arranged by size: 4090 1005 709 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2820 4009 1353 Size: 1467 1189 3423 Fragments arranged by size: 3423 1467 1189 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4666 5040 5186 387 Size: 1064 138 3077 374 146 1280 Fragments arranged by size: 3077 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2816 3331 4142 4739 5526 5794 1239 Size: 393 1184 515 811 597 787 268 1524 Fragments arranged by size: 1524 1184 811 787 597 515 393 268 EcoO109I rG'GnC_Cy Cuts at: 2658 2658 Size: 6079 EcoRI G'AATT_C Cuts at: 3388 3766 3388 Size: 378 5701 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2946 2946 Size: 6079 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 5226 1678 Size: 3548 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2304 2336 4720 4752 4794 Size: 32 135 32 732 32 2384 32 42 Cuts at: 4794 4826 1373 Size: 32 2626 Fragments arranged by size: 2626 2384 732 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3244 3423 3669 669 Size: 988 281 1306 179 246 3079 Fragments arranged by size: 3079 1306 988 281 246 179 HindIII A'AGCT_T Cuts at: 748 2736 2928 3047 748 Size: 1988 192 119 3780 Fragments arranged by size: 3780 1988 192 119 HpaI GTT'AAC Cuts at: 3244 3669 3244 Size: 425 5654 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3168 5193 5377 1305 Size: 1863 2025 184 2007 Fragments arranged by size: 2025 2007 1863 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2170 2365 10 Size: 54 1425 343 338 195 3724 Fragments arranged by size: 3724 1425 343 338 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2573 3261 4037 4396 4555 518 Size: 648 1056 351 688 776 359 159 2042 Fragments arranged by size: 2042 1056 776 688 648 359 351 159 MspA1I CmG'CkG Cuts at: 1606 2683 3495 4172 5320 5565 1606 Size: 1077 812 677 1148 245 2120 Fragments arranged by size: 2120 1148 1077 812 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 3437 4982 1132 Size: 97 2208 1545 2229 Fragments arranged by size: 2229 2208 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6079 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2658 2658 Size: 6079 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 3165 3649 3165 Size: 484 5595 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3098 3130 3098 Size: 32 6047 PstI C_TGCA'G Cuts at: 830 3431 830 Size: 2601 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SfcI C'TryA_G Cuts at: 826 1045 2100 2550 3427 4562 5243 5434 826 Size: 219 1055 450 877 1135 681 191 1471 Fragments arranged by size: 1471 1135 1055 877 681 450 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4183 5084 5346 820 Size: 197 926 2240 901 262 1553 Fragments arranged by size: 2240 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2474 2703 3870 493 Size: 1981 229 1167 2702 Fragments arranged by size: 2702 1981 1167 229 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 2998 4003 5 Size: 47 2946 1005 2081 Fragments arranged by size: 2946 2081 1005 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6079 StyI C'CwwG_G Cuts at: 513 1065 2004 2891 2941 3518 513 Size: 552 939 887 50 577 3074 Fragments arranged by size: 3074 939 887 577 552 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4106 4123 4276 4461 4906 325 Size: 1255 2526 17 153 185 445 1498 Fragments arranged by size: 2526 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4325 6037 96 Size: 274 80 33 51 1443 2348 1712 138 Fragments arranged by size: 2348 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3358 3390 6039 6071 3358 Size: 32 2649 32 3366 Fragments arranged by size: 3366 2649 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2781 1316 Size: 1465 4614 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 2929 3048 4208 2929 Size: 119 1160 4800 Fragments arranged by size: 4800 1160 119 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2888 3887 276 Size: 53 83 186 936 1354 999 2468 Fragments arranged by size: 2468 1354 999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI