Restriction map for
pFN21AB6688
(Linear) (Six-base) MAPSORT of: KIBB6688.seq Check: 1274 from: 1 to: 1374
KIBB6688.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 0 885 1374
Size: 885 489
AccI GT'mk_AC
Cuts at: 0 1102 1374
Size: 1102 272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1374
Size: 811 563
AgeI A'CCGG_T
Cuts at: 0 1263 1374
Size: 1263 111
AhdI GACnn_n'nnGTC
Cuts at: 0 313 1374
Size: 313 1061
ApaLI G'TGCA_C
Cuts at: 0 37 1374
Size: 37 1337
ApoI r'AATT_y
Cuts at: 0 139 636 1118 1317 1374
Size: 139 497 482 199 57
Fragments arranged by size:
497 482 199 139 57
AvrII C'CTAG_G
Cuts at: 0 886 1374
Size: 886 488
BanI G'GyrC_C
Cuts at: 0 892 971 1374
Size: 892 79 403
Fragments arranged by size:
892 403 79
BanII G_rGCy'C
Cuts at: 0 85 1295 1374
Size: 85 1210 79
Fragments arranged by size:
1210 85 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 603 637 1374
Size: 603 34 737
Fragments arranged by size:
737 603 34
BclI T'GATC_A
Cuts at: 0 221 962 1374
Size: 221 741 412
Fragments arranged by size:
741 412 221
Bme1580I G_kGCm'C
Cuts at: 0 41 382 895 976 1374
Size: 41 341 513 81 398
Fragments arranged by size:
513 398 341 81 41
BmgBI CAC'GTC
Cuts at: 0 617 1374
Size: 617 757
BmrI ACTGGGnnnn_n'
Cuts at: 0 79 1374
Size: 79 1295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1326 1374
Size: 1326 48
BsaAI yAC'GTr
Cuts at: 0 469 698 1374
Size: 469 229 676
Fragments arranged by size:
676 469 229
BsaHI Gr'CG_yC
Cuts at: 0 882 1374
Size: 882 492
BsaWI w'CCGG_w
Cuts at: 0 1263 1374
Size: 1263 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 730 1374
Size: 235 495 644
Fragments arranged by size:
644 495 235
BsiEI CG_ry'CG
Cuts at: 0 815 1374
Size: 815 559
BsiHKAI G_wGCw'C
Cuts at: 0 41 626 1374
Size: 41 585 748
Fragments arranged by size:
748 585 41
BsmI GAATG_Cn'
Cuts at: 0 21 24 1132 1374
Size: 21 3 1108 242
Fragments arranged by size:
1108 242 21 3
BsmBI CGTCTCn'nnnn_
Cuts at: 0 873 1374
Size: 873 501
Bsp1286I G_dGCh'C
Cuts at: 0 41 85 382 626 895 976 1295 1374
Size: 41 44 297 244 269 81 319 79
Fragments arranged by size:
319 297 269 244 81 79 44 41
BspHI T'CATG_A
Cuts at: 0 194 515 1374
Size: 194 321 859
Fragments arranged by size:
859 321 194
BsrDI GCAATG_nn'
Cuts at: 0 287 1374
Size: 287 1087
BsrFI r'CCGG_y
Cuts at: 0 1263 1374
Size: 1263 111
BstBI TT'CG_AA
Cuts at: 0 928 1374
Size: 928 446
BstZ17I GTA'TAC
Cuts at: 0 1103 1374
Size: 1103 271
Bsu36I CC'TnA_GG
Cuts at: 0 10 1374
Size: 10 1364
BtsI GCAGTG_nn'
Cuts at: 0 1208 1374
Size: 1208 166
DraIII CAC_nnn'GTG
Cuts at: 0 617 1374
Size: 617 757
EaeI y'GGCC_r
Cuts at: 0 163 358 750 812 1374
Size: 163 195 392 62 562
Fragments arranged by size:
562 392 195 163 62
EagI C'GGCC_G
Cuts at: 0 812 1374
Size: 812 562
EarI CTCTTCn'nnn_
Cuts at: 0 815 1374
Size: 815 559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1326 1374
Size: 811 515 48
Fragments arranged by size:
811 515 48
EcoO109I rG'GnC_Cy
Cuts at: 0 653 1374
Size: 653 721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 941 1374
Size: 941 433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 1374
Size: 299 32 1043
Fragments arranged by size:
1043 299 32
HincII GTy'rAC
Cuts at: 0 1239 1374
Size: 1239 135
HindIII A'AGCT_T
Cuts at: 0 731 923 1042 1374
Size: 731 192 119 332
Fragments arranged by size:
731 332 192 119
HpaI GTT'AAC
Cuts at: 0 1239 1374
Size: 1239 135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1374
Size: 1163 211
MscI TGG'CCA
Cuts at: 0 165 360 1374
Size: 165 195 1014
Fragments arranged by size:
1014 195 165
MslI CAynn'nnrTG
Cuts at: 0 217 568 1256 1374
Size: 217 351 688 118
Fragments arranged by size:
688 351 217 118
MspA1I CmG'CkG
Cuts at: 0 678 1374
Size: 678 696
PpuMI rG'GwC_Cy
Cuts at: 0 653 1374
Size: 653 721
PsiI TTA'TAA
Cuts at: 0 1160 1374
Size: 1160 214
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1093 1125 1374
Size: 1093 32 249
Fragments arranged by size:
1093 249 32
SfcI C'TryA_G
Cuts at: 0 95 545 1374
Size: 95 450 829
Fragments arranged by size:
829 450 95
SnaBI TAC'GTA
Cuts at: 0 469 698 1374
Size: 469 229 676
Fragments arranged by size:
676 469 229
SspI AAT'ATT
Cuts at: 0 993 1374
Size: 993 381
StyI C'CwwG_G
Cuts at: 0 886 936 1374
Size: 886 50 438
Fragments arranged by size:
886 438 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1353 1374
Size: 1353 21
Tth111I GACn'n_nGTC
Cuts at: 0 776 1374
Size: 776 598
XmnI GAAnn'nnTTC
Cuts at: 0 924 1043 1374
Size: 924 119 331
Fragments arranged by size:
924 331 119
ZraI GAC'GTC
Cuts at: 0 883 1374
Size: 883 491
Enzymes that do cut:
AatII AccI AcuI AgeI AhdI ApaLI ApoI AvrII
BanI BanII BcgI BclI Bme1580I BmgBI BmrI BpmI
BsaAI BsaHI BsaWI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrDI BsrFI BstBI BstZ17I Bsu36I BtsI
DraIII EaeI EagI EarI Eco57MI EcoO109I FalI Hin4I
HincII HindIII HpaI MmeI MscI MslI MspA1I PpuMI
PsiI PsrI SfcI SnaBI SspI StyI TstI Tth111I
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AleI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvaI BaeI
BamHI BbeI BbsI BbvCI BciVI BfrBI BglI BglII
BlpI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaXI
BseRI BseYI BsiWI BspEI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI ClaI
CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI StuI SwaI TaqII TatI XbaI XcmI
XhoI XmaI