Restriction map for
pFN21AB0437
(Linear) (Six-base) MAPSORT of: KIBB0437.seq Check: 3909 from: 1 to: 1443
KIBB0437.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
Acc65I G'GTAC_C
Cuts at: 0 613 1443
Size: 613 830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 853 902 937 1138 1336 1443
Size: 836 17 49 35 201 198 107
Fragments arranged by size:
836 201 198 107 49 35 17
AflIII A'CryG_T
Cuts at: 0 821 1443
Size: 821 622
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 1073 1105 1443
Size: 313 32 728 32 338
Fragments arranged by size:
728 338 313 32 32
ApoI r'AATT_y
Cuts at: 0 455 829 1039 1443
Size: 455 374 210 404
Fragments arranged by size:
455 404 374 210
AvaI C'yCGr_G
Cuts at: 0 138 1443
Size: 138 1305
BamHI G'GATC_C
Cuts at: 0 359 1443
Size: 359 1084
BanI G'GyrC_C
Cuts at: 0 613 1375 1443
Size: 613 762 68
Fragments arranged by size:
762 613 68
BanII G_rGCy'C
Cuts at: 0 420 636 877 1050 1443
Size: 420 216 241 173 393
Fragments arranged by size:
420 393 241 216 173
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 1443
Size: 824 619
BciVI GTATCCnnnnn_n'
Cuts at: 0 1020 1443
Size: 1020 423
BclI T'GATC_A
Cuts at: 0 1205 1443
Size: 1205 238
BfrBI ATG'CAT
Cuts at: 0 1013 1443
Size: 1013 430
BglII A'GATC_T
Cuts at: 0 837 1319 1443
Size: 837 482 124
Fragments arranged by size:
837 482 124
Bme1580I G_kGCm'C
Cuts at: 0 1378 1443
Size: 1378 65
BmgBI CAC'GTC
Cuts at: 0 822 902 1443
Size: 822 80 541
Fragments arranged by size:
822 541 80
BmrI ACTGGGnnnn_n'
Cuts at: 0 170 1381 1443
Size: 170 1211 62
Fragments arranged by size:
1211 170 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 434 1106 1382 1443
Size: 100 334 672 276 61
Fragments arranged by size:
672 334 276 100 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 989 1443
Size: 989 454
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 43 58 73 1443
Size: 28 15 15 15 1370
Fragments arranged by size:
1370 28 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 103 943 1443
Size: 78 25 840 500
Fragments arranged by size:
840 500 78 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 459 704 1443
Size: 234 225 245 739
Fragments arranged by size:
739 245 234 225
BsiHKAI G_wGCw'C
Cuts at: 0 636 1050 1077 1443
Size: 636 414 27 366
Fragments arranged by size:
636 414 366 27
Bsp1286I G_dGCh'C
Cuts at: 0 420 636 877 1050 1077 1378 1443
Size: 420 216 241 173 27 301 65
Fragments arranged by size:
420 301 241 216 173 65 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1138 1443
Size: 1138 305
BsrDI GCAATG_nn'
Cuts at: 0 672 939 1443
Size: 672 267 504
Fragments arranged by size:
672 504 267
BsrFI r'CCGG_y
Cuts at: 0 1348 1443
Size: 1348 95
BstYI r'GATC_y
Cuts at: 0 108 359 837 1319 1443
Size: 108 251 478 482 124
Fragments arranged by size:
482 478 251 124 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 758 1443
Size: 758 685
BtsI GCAGTG_nn'
Cuts at: 0 692 1359 1443
Size: 692 667 84
Fragments arranged by size:
692 667 84
DraIII CAC_nnn'GTG
Cuts at: 0 437 450 1443
Size: 437 13 993
Fragments arranged by size:
993 437 13
DrdI GACnn_nn'nnGTC
Cuts at: 0 187 1443
Size: 187 1256
EaeI y'GGCC_r
Cuts at: 0 474 1343 1443
Size: 474 869 100
Fragments arranged by size:
869 474 100
EarI CTCTTCn'nnn_
Cuts at: 0 96 340 872 1443
Size: 96 244 532 571
Fragments arranged by size:
571 532 244 96
EcoICRI GAG'CTC
Cuts at: 0 634 1048 1443
Size: 634 414 395
Fragments arranged by size:
634 414 395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 434 836 853 902 937 1106 1138
Size: 100 334 402 17 49 35 169 32
Cuts at: 1138 1336 1382 1443
Size: 198 46 61
Fragments arranged by size:
402 334 198 169 100 61 49 46
35 32 17
EcoO109I rG'GnC_Cy
Cuts at: 0 135 1081 1443
Size: 135 946 362
Fragments arranged by size:
946 362 135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 859 1443
Size: 859 584
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 370 402 1443
Size: 28 32 310 32 1041
Fragments arranged by size:
1041 310 32 32 28
HindIII A'AGCT_T
Cuts at: 0 884 1443
Size: 884 559
KpnI G_GTAC'C
Cuts at: 0 617 1443
Size: 617 826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1443
Size: 296 1147
MscI TGG'CCA
Cuts at: 0 476 1443
Size: 476 967
MslI CAynn'nnrTG
Cuts at: 0 1226 1443
Size: 1226 217
MspA1I CmG'CkG
Cuts at: 0 99 370 1392 1443
Size: 99 271 1022 51
Fragments arranged by size:
1022 271 99 51
NaeI GCC'GGC
Cuts at: 0 1350 1443
Size: 1350 93
NdeI CA'TA_TG
Cuts at: 0 1015 1200 1443
Size: 1015 185 243
Fragments arranged by size:
1015 243 185
NgoMIV G'CCGG_C
Cuts at: 0 1348 1443
Size: 1348 95
NsiI A_TGCA'T
Cuts at: 0 1015 1443
Size: 1015 428
NspI r_CATG'y
Cuts at: 0 303 513 1443
Size: 303 210 930
Fragments arranged by size:
930 303 210
PasI CC'CwG_GG
Cuts at: 0 64 1443
Size: 64 1379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 1443
Size: 454 32 957
Fragments arranged by size:
957 454 32
PpuMI rG'GwC_Cy
Cuts at: 0 135 1081 1443
Size: 135 946 362
Fragments arranged by size:
946 362 135
PstI C_TGCA'G
Cuts at: 0 702 1443
Size: 702 741
PvuII CAG'CTG
Cuts at: 0 99 370 1443
Size: 99 271 1073
Fragments arranged by size:
1073 271 99
SacI G_AGCT'C
Cuts at: 0 636 1050 1443
Size: 636 414 393
Fragments arranged by size:
636 414 393
SapI GCTCTTCn'nnn_
Cuts at: 0 872 1443
Size: 872 571
SfcI C'TryA_G
Cuts at: 0 283 698 1443
Size: 283 415 745
Fragments arranged by size:
745 415 283
StuI AGG'CCT
Cuts at: 0 10 1443
Size: 10 1433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 43 75 1443
Size: 43 32 1368
Fragments arranged by size:
1368 43 32
XbaI T'CTAG_A
Cuts at: 0 555 1443
Size: 555 888
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 159 1443
Size: 159 1284
XmnI GAAnn'nnTTC
Cuts at: 0 839 866 1443
Size: 839 27 577
Fragments arranged by size:
839 577 27
Enzymes that do cut:
Acc65I AcuI AflIII AloI ApoI AvaI BamHI BanI
BanII BbsI BciVI BclI BfrBI BglII Bme1580I BmgBI
BmrI BpmI BsaI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BstYI BtgZI BtsI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HindIII
KpnI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV
NsiI NspI PasI PpiI PpuMI PstI PvuII SacI
SapI SfcI StuI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BglI BlpI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
MfeI MluI NarI NcoI NheI NotI NruI PacI
PciI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
TatI Tth111I XhoI XmaI ZraI