(Circular) (Six-base) MAPSORT of: pFN21AB6407.seq Check: 1492 from: 1 to: 5623 pFN21AB6407 5623 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2511 3433 278 Size: 53 83 186 936 975 922 2468 Fragments arranged by size: 2468 975 936 922 186 83 53 AccI GT'mk_AC Cuts at: 1937 2966 1937 Size: 1029 4594 Acc65I G'GTAC_C Cuts at: 2944 2944 Size: 5623 AclI AA'CG_TT Cuts at: 1563 3750 4123 1563 Size: 2187 373 3063 Fragments arranged by size: 3063 2187 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2897 3686 5070 5338 1632 Size: 1265 789 1384 268 1917 Fragments arranged by size: 1917 1384 1265 789 268 AfeI AGC'GCT Cuts at: 2711 2711 Size: 5623 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5426 AflIII A'CryG_T Cuts at: 1203 4522 5499 1203 Size: 3319 977 1327 Fragments arranged by size: 3319 1327 977 AgeI A'CCGG_T Cuts at: 4454 4454 Size: 5623 AhdI GACnn_n'nnGTC Cuts at: 4352 4352 Size: 5623 AleI CACnn'nnGTG Cuts at: 1166 2783 1166 Size: 1617 4006 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2801 1184 Size: 1617 4006 AlwNI CAG_nnn'CTG Cuts at: 3366 4938 3366 Size: 1572 4051 ApaLI G'TGCA_C Cuts at: 3680 4836 3680 Size: 1156 4467 ApoI r'AATT_y Cuts at: 1493 2135 2932 3158 3310 5419 1493 Size: 642 797 226 152 2109 1697 Fragments arranged by size: 2109 1697 797 642 226 152 AseI AT'TA_AT Cuts at: 160 1032 4177 160 Size: 872 3145 1606 Fragments arranged by size: 3145 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2643 2948 1870 Size: 73 123 577 305 4545 Fragments arranged by size: 4545 577 305 123 73 BamHI G'GATC_C Cuts at: 2049 2953 3320 2049 Size: 904 367 4352 Fragments arranged by size: 4352 904 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2785 2944 4399 618 Size: 325 217 627 998 159 1455 1842 Fragments arranged by size: 1842 1455 998 627 325 217 159 BanII G_rGCy'C Cuts at: 1391 2942 1391 Size: 1551 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4652 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2047 2081 3814 3848 1903 Size: 34 110 34 1733 34 3678 Fragments arranged by size: 3678 1733 110 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3515 4725 3515 Size: 1210 4413 BclI T'GATC_A Cuts at: 1548 1548 Size: 5623 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4234 136 Size: 107 122 71 631 605 2562 1525 Fragments arranged by size: 2562 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2293 2912 5618 2293 Size: 619 2706 2298 Fragments arranged by size: 2706 2298 619 BlpI GC'TnA_GC Cuts at: 2546 2774 3040 2546 Size: 228 266 5129 Fragments arranged by size: 5129 266 228 Bme1580I G_kGCm'C Cuts at: 1790 2790 3684 4840 1790 Size: 1000 894 1156 2573 Fragments arranged by size: 2573 1156 1000 894 BmgBI CAC'GTC Cuts at: 1318 2570 1318 Size: 1252 4371 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2400 3354 4312 Size: 416 53 83 399 24 983 954 958 Cuts at: 4312 442 Size: 1753 Fragments arranged by size: 1753 983 958 954 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5623 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2546 2546 Size: 5623 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2309 2726 4283 1239 Size: 911 159 417 1557 2579 Fragments arranged by size: 2579 1557 911 417 159 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2318 3748 4613 4911 2318 Size: 1430 865 298 3030 Fragments arranged by size: 3030 1430 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4286 882 Size: 617 2787 2219 Fragments arranged by size: 2787 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2044 2580 3414 493 Size: 711 840 536 834 2702 Fragments arranged by size: 2702 840 834 711 536 BsaBI GATnn'nnATC Cuts at: 5617 5617 Size: 5623 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2062 2508 3430 3812 Size: 53 83 186 936 529 446 922 382 Cuts at: 3812 4447 275 Size: 635 1451 Fragments arranged by size: 1451 936 922 635 529 446 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2052 4056 4454 4728 4875 5286 2052 Size: 2004 398 274 147 411 2389 Fragments arranged by size: 2389 2004 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2252 2282 2593 2623 1178 Size: 30 165 30 849 30 311 30 4178 Fragments arranged by size: 4178 849 311 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5623 BseYI C'CCAG_C Cuts at: 4826 5322 4826 Size: 496 5127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5050 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3073 3834 4862 1540 Size: 275 186 1072 761 1028 2301 Fragments arranged by size: 2301 1072 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2780 2922 3684 3769 4840 2780 Size: 142 762 85 1071 3563 Fragments arranged by size: 3563 1071 762 142 85 BsmI GAATG_Cn' Cuts at: 3134 3227 3134 Size: 93 5530 BsmBI CGTCTCn'nnnn_ Cuts at: 2640 2640 Size: 5623 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2780 2790 2922 2942 3684 3769 4840 Size: 399 990 10 132 20 742 85 1071 Cuts at: 4840 1391 Size: 2174 Fragments arranged by size: 2174 1071 990 742 399 132 85 20 10 BspEI T'CCGG_A Cuts at: 2052 5286 2052 Size: 3234 2389 BspHI T'CATG_A Cuts at: 3512 5242 3512 Size: 1730 3893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2978 844 Size: 2134 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2761 3510 1394 Size: 429 938 749 3507 Fragments arranged by size: 3507 938 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4118 4292 65 Size: 1183 2870 174 1396 Fragments arranged by size: 2870 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4267 4454 1700 Size: 110 2457 187 2869 Fragments arranged by size: 2869 2457 187 110 BsrGI T'GTAC_A Cuts at: 96 2455 96 Size: 2359 3264 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5623 BssSI C'ACGA_G Cuts at: 2705 3683 4695 2705 Size: 978 1012 3633 Fragments arranged by size: 3633 1012 978 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5623 BstBI TT'CG_AA Cuts at: 4427 4427 Size: 5623 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5623 BstYI r'GATC_y Cuts at: 1972 2049 2293 2912 2953 3320 3705 3722 5174 Size: 77 244 619 41 367 385 17 1452 Cuts at: 5174 5260 5272 5618 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 619 385 367 346 244 86 77 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2781 513 Size: 552 939 777 3355 Fragments arranged by size: 3355 939 777 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2095 487 Size: 683 56 869 4015 Fragments arranged by size: 4015 869 683 56 BtsI GCAGTG_nn' Cuts at: 3139 3933 3953 3139 Size: 794 20 4809 Fragments arranged by size: 4809 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5623 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2377 2412 529 Size: 35 1813 35 3740 Fragments arranged by size: 3740 1813 35 35 DraI TTT'AAA Cuts at: 2927 2927 Size: 5623 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4630 809 Size: 507 3314 1802 Fragments arranged by size: 3314 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2385 2540 2688 Size: 54 1425 50 275 18 555 155 148 Cuts at: 2688 3070 3959 5588 8 Size: 382 889 1629 43 Fragments arranged by size: 1629 1425 889 555 382 275 155 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3070 1537 Size: 275 1258 4090 Fragments arranged by size: 4090 1258 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3553 1353 Size: 2200 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4210 4584 4730 387 Size: 1064 138 2621 374 146 1280 Fragments arranged by size: 2621 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2309 2726 2897 3686 4283 5070 Size: 393 518 159 417 171 789 597 787 Cuts at: 5070 5338 1239 Size: 268 1524 Fragments arranged by size: 1524 789 787 597 518 417 393 268 171 159 EcoRI G'AATT_C Cuts at: 2932 3310 2932 Size: 378 5245 FspI TGC'GCA Cuts at: 4129 4129 Size: 5623 HaeII r_GCGC'y Cuts at: 1678 2553 2713 2805 4770 1678 Size: 875 160 92 1965 2531 Fragments arranged by size: 2531 1965 875 160 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2425 2457 4264 Size: 32 135 32 680 32 141 32 1807 Cuts at: 4264 4296 4338 4370 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1807 680 141 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2967 3213 669 Size: 988 281 1029 246 3079 Fragments arranged by size: 3079 1029 988 281 246 HindIII A'AGCT_T Cuts at: 748 2103 2879 748 Size: 1355 776 3492 Fragments arranged by size: 3492 1355 776 HpaI GTT'AAC Cuts at: 3213 3213 Size: 5623 KpnI G_GTAC'C Cuts at: 2948 2948 Size: 5623 MfeI C'AATT_G Cuts at: 3222 3222 Size: 5623 MluI A'CGCG_T Cuts at: 5499 5499 Size: 5623 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4737 4921 1305 Size: 3432 184 2007 Fragments arranged by size: 3432 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2387 2690 10 Size: 54 1425 343 555 303 2943 Fragments arranged by size: 2943 1425 555 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2783 3581 3940 4099 518 Size: 648 1617 798 359 159 2042 Fragments arranged by size: 2042 1617 798 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3039 3716 4864 5109 1606 Size: 1433 677 1148 245 2120 Fragments arranged by size: 2120 1433 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2781 513 Size: 552 939 777 3355 Fragments arranged by size: 3355 939 777 552 NdeI CA'TA_TG Cuts at: 387 2485 387 Size: 2098 3525 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5623 NotI GC'GGCC_GC Cuts at: 3070 3070 Size: 5623 NspI r_CATG'y Cuts at: 1132 1229 2240 2981 4526 1132 Size: 97 1011 741 1545 2229 Fragments arranged by size: 2229 1545 1011 741 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5623 PciI A'CATG_T Cuts at: 4522 4522 Size: 5623 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5623 PmeI GTTT'AAAC Cuts at: 2927 2927 Size: 5623 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3692 3724 5205 5237 3692 Size: 32 1481 32 4078 Fragments arranged by size: 4078 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5623 PsiI TTA'TAA Cuts at: 3193 3193 Size: 5623 PstI C_TGCA'G Cuts at: 830 2975 830 Size: 2145 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5623 SalI G'TCGA_C Cuts at: 1936 2965 1936 Size: 1029 4594 SbfI CC_TGCA'GG Cuts at: 2975 2975 Size: 5623 ScaI AGT'ACT Cuts at: 2322 3871 5583 2322 Size: 1549 1712 2362 Fragments arranged by size: 2362 1712 1549 SfcI C'TryA_G Cuts at: 826 1045 2717 2908 2971 4106 4787 4978 826 Size: 219 1672 191 63 1135 681 191 1471 Fragments arranged by size: 1672 1471 1135 681 219 191 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5623 SmaI CCC'GGG Cuts at: 1872 2950 1872 Size: 1078 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2297 3727 4628 4890 820 Size: 197 926 354 1430 901 262 1553 Fragments arranged by size: 1553 1430 926 901 354 262 197 SnaBI TAC'GTA Cuts at: 493 3414 493 Size: 2921 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5623 SphI G_CATG'C Cuts at: 1132 2981 1132 Size: 1849 3774 SspI AAT'ATT Cuts at: 5 52 3547 5 Size: 47 3495 2081 Fragments arranged by size: 3495 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5623 StyI C'CwwG_G Cuts at: 513 1065 2004 2365 2685 2781 3062 513 Size: 552 939 361 320 96 281 3074 Fragments arranged by size: 3074 939 552 361 320 281 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3650 3667 3820 4005 4450 325 Size: 1255 2070 17 153 185 445 1498 Fragments arranged by size: 2070 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2320 2455 3869 Size: 274 80 33 51 1443 343 135 1414 Cuts at: 3869 5581 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1414 343 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2593 2625 5583 5615 2593 Size: 32 2958 32 2601 Fragments arranged by size: 2958 2601 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5623 XbaI T'CTAG_A Cuts at: 2959 2959 Size: 5623 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5623 XmaI C'CCGG_G Cuts at: 1870 2948 1870 Size: 1078 4545 XmnI GAAnn'nnTTC Cuts at: 3752 3752 Size: 5623 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2509 3431 276 Size: 53 83 186 936 975 922 2468 Fragments arranged by size: 2468 975 936 922 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI