(Circular) (Six-base) MAPSORT of: pFN21AB6242.seq Check: 7225 from: 1 to: 6766 pFN21AB6242 6766 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3088 3088 Size: 6766 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4576 278 Size: 53 83 186 936 3040 2468 Fragments arranged by size: 3040 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3056 3962 4109 1937 Size: 1119 906 147 4594 Fragments arranged by size: 4594 1119 906 147 Acc65I G'GTAC_C Cuts at: 3759 4087 3759 Size: 328 6438 AclI AA'CG_TT Cuts at: 1563 4893 5266 1563 Size: 3330 373 3063 Fragments arranged by size: 3330 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3251 3458 4050 4829 6213 6481 1632 Size: 1619 207 592 779 1384 268 1917 Fragments arranged by size: 1917 1619 1384 779 592 268 207 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6569 AflIII A'CryG_T Cuts at: 1203 5665 6642 1203 Size: 4462 977 1327 Fragments arranged by size: 4462 1327 977 AgeI A'CCGG_T Cuts at: 5597 5597 Size: 6766 AhdI GACnn_n'nnGTC Cuts at: 3958 5495 3958 Size: 1537 5229 AleI CACnn'nnGTG Cuts at: 1166 2407 1166 Size: 1241 5525 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6766 AlwNI CAG_nnn'CTG Cuts at: 2464 4509 6081 2464 Size: 2045 1572 3149 Fragments arranged by size: 3149 2045 1572 ApaLI G'TGCA_C Cuts at: 3485 4823 5979 3485 Size: 1338 1156 4272 Fragments arranged by size: 4272 1338 1156 ApoI r'AATT_y Cuts at: 1493 2179 2673 2952 3295 3836 3969 4075 4301 Size: 686 494 279 343 541 133 106 226 Cuts at: 4301 4453 6562 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 686 541 494 343 279 226 152 133 106 AseI AT'TA_AT Cuts at: 160 1032 5320 160 Size: 872 4288 1606 Fragments arranged by size: 4288 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6766 AvaI C'yCGr_G Cuts at: 1870 1943 4091 1870 Size: 73 2148 4545 Fragments arranged by size: 4545 2148 73 BamHI G'GATC_C Cuts at: 2020 2137 2156 4096 4463 2020 Size: 117 19 1940 367 4323 Fragments arranged by size: 4323 1940 367 117 19 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 3759 4087 5542 618 Size: 325 217 627 251 1721 328 1455 1842 Fragments arranged by size: 1842 1721 1455 627 328 325 251 217 BanII G_rGCy'C Cuts at: 1391 2586 3785 4085 1391 Size: 1195 1199 300 4072 Fragments arranged by size: 4072 1199 1195 300 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2846 3000 3401 3918 928 Size: 971 947 154 401 517 3776 Fragments arranged by size: 3776 971 947 517 401 154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4957 4991 1903 Size: 34 3020 34 3678 Fragments arranged by size: 3678 3020 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2789 4658 5868 2789 Size: 1869 1210 3687 Fragments arranged by size: 3687 1869 1210 BclI T'GATC_A Cuts at: 1548 3180 1548 Size: 1632 5134 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5377 136 Size: 107 122 71 631 605 3705 1525 Fragments arranged by size: 3705 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3496 6761 3496 Size: 3265 3501 BlpI GC'TnA_GC Cuts at: 4183 4183 Size: 6766 Bme1580I G_kGCm'C Cuts at: 1790 3489 4827 5983 1790 Size: 1699 1338 1156 2573 Fragments arranged by size: 2573 1699 1338 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6766 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4497 5455 442 Size: 416 53 83 399 24 3080 958 1753 Fragments arranged by size: 3080 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6766 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2566 3804 2566 Size: 1238 5528 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 3450 3506 5426 1239 Size: 1262 949 56 1920 2579 Fragments arranged by size: 2579 1920 1262 949 56 Bpu10I CC'TnA_GC Cuts at: 2319 2319 Size: 6766 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3933 4891 5756 6054 3933 Size: 958 865 298 4645 Fragments arranged by size: 4645 958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2468 2507 3570 5429 882 Size: 617 969 39 1063 1859 2219 Fragments arranged by size: 2219 1859 1063 969 617 39 BsaAI yAC'GTr Cuts at: 493 1204 4557 493 Size: 711 3353 2702 Fragments arranged by size: 3353 2702 711 BsaBI GATnn'nnATC Cuts at: 3150 6760 3150 Size: 3610 3156 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3717 4573 4955 5590 Size: 53 83 186 936 2184 856 382 635 Cuts at: 5590 275 Size: 1451 Fragments arranged by size: 2184 1451 936 856 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3546 5199 5597 5871 6018 6429 3546 Size: 1653 398 274 147 411 3883 Fragments arranged by size: 3883 1653 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2022 2052 3080 3110 1178 Size: 30 165 30 619 30 1028 30 4834 Fragments arranged by size: 4834 1028 619 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2257 2462 2498 3572 3824 4010 1186 Size: 1071 205 36 1074 252 186 3942 Fragments arranged by size: 3942 1074 1071 252 205 186 36 BseYI C'CCAG_C Cuts at: 5969 6465 5969 Size: 496 6270 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3513 3777 1163 Size: 573 1777 264 4152 Fragments arranged by size: 4152 1777 573 264 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4216 4977 6005 1540 Size: 275 186 2215 761 1028 2301 Fragments arranged by size: 2301 2215 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2389 2586 3489 3770 4827 4912 5983 2389 Size: 197 903 281 1057 85 1071 3172 Fragments arranged by size: 3172 1071 1057 903 281 197 85 BsmI GAATG_Cn' Cuts at: 4277 4370 4277 Size: 93 6673 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2389 2586 3489 3770 3785 4085 4827 Size: 399 599 197 903 281 15 300 742 Cuts at: 4827 4912 5983 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 903 742 599 399 300 281 197 85 15 BspEI T'CCGG_A Cuts at: 6429 6429 Size: 6766 BspHI T'CATG_A Cuts at: 4655 6385 4655 Size: 1730 5036 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3088 4121 844 Size: 2244 1033 3489 Fragments arranged by size: 3489 2244 1033 BsrBI CCG'CTC Cuts at: 1394 1823 4653 1394 Size: 429 2830 3507 Fragments arranged by size: 3507 2830 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2271 5261 5435 65 Size: 1183 1023 2990 174 1396 Fragments arranged by size: 2990 1396 1183 1023 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2889 3816 5410 5597 1700 Size: 110 1079 927 1594 187 2869 Fragments arranged by size: 2869 1594 1079 927 187 110 BsrGI T'GTAC_A Cuts at: 96 3210 96 Size: 3114 3652 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6766 BssSI C'ACGA_G Cuts at: 4826 5838 4826 Size: 1012 5754 BstAPI GCAn_nnn'nTGC Cuts at: 794 3128 794 Size: 2334 4432 BstBI TT'CG_AA Cuts at: 5570 5570 Size: 6766 BstEII G'GTnAC_C Cuts at: 1184 2885 1184 Size: 1701 5065 BstYI r'GATC_y Cuts at: 1972 2020 2137 2156 3496 4096 4463 4848 4865 Size: 48 117 19 1340 600 367 385 17 Cuts at: 4865 6317 6403 6415 6761 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1340 600 385 367 346 117 86 48 19 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 3636 513 Size: 552 939 30 1602 3643 Fragments arranged by size: 3643 1602 939 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6027 Fragments arranged by size: 6027 683 56 BtsI GCAGTG_nn' Cuts at: 3931 4282 5076 5096 3931 Size: 351 794 20 5601 Fragments arranged by size: 5601 794 351 20 ClaI AT'CG_AT Cuts at: 1554 2218 1554 Size: 664 6102 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6731 DraI TTT'AAA Cuts at: 2563 2950 3259 4070 2563 Size: 387 309 811 5259 Fragments arranged by size: 5259 811 387 309 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5773 809 Size: 507 4457 1802 Fragments arranged by size: 4457 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 2466 4213 Size: 54 1425 50 275 18 498 138 1747 Cuts at: 4213 5102 6731 8 Size: 889 1629 43 Fragments arranged by size: 1747 1629 1425 889 498 275 138 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4213 1537 Size: 275 2401 4090 Fragments arranged by size: 4090 2401 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3987 4008 4696 1353 Size: 2634 21 688 3423 Fragments arranged by size: 3423 2634 688 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3557 5353 5727 5873 387 Size: 1064 138 1968 1796 374 146 1280 Fragments arranged by size: 1968 1796 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2584 2584 Size: 6766 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2501 3251 3450 3458 3506 4050 4829 Size: 393 869 750 199 8 48 544 779 Cuts at: 4829 5426 6213 6481 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 869 787 779 750 597 544 393 268 199 48 8 EcoNI CCTnn'n_nnAGG Cuts at: 2822 2822 Size: 6766 EcoRI G'AATT_C Cuts at: 4075 4453 4075 Size: 378 6388 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2834 3336 2834 Size: 502 6264 FspI TGC'GCA Cuts at: 5272 5272 Size: 6766 HaeII r_GCGC'y Cuts at: 1678 5913 1678 Size: 4235 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2293 2325 2560 2592 2621 Size: 32 135 32 721 32 235 32 29 Cuts at: 2621 2653 3857 3889 3944 3976 5407 5439 5481 Size: 32 1204 32 55 32 1431 32 42 Cuts at: 5481 5513 1373 Size: 32 2626 Fragments arranged by size: 2626 1431 1204 721 235 135 55 42 32 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 2195 4110 4356 669 Size: 988 281 257 1915 246 3079 Fragments arranged by size: 3079 1915 988 281 257 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6766 HpaI GTT'AAC Cuts at: 4356 4356 Size: 6766 KpnI G_GTAC'C Cuts at: 3763 4091 3763 Size: 328 6438 MfeI C'AATT_G Cuts at: 4365 4365 Size: 6766 MluI A'CGCG_T Cuts at: 6642 6642 Size: 6766 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2247 2577 2708 5880 6064 1305 Size: 942 330 131 3172 184 2007 Fragments arranged by size: 3172 2007 942 330 184 131 MscI TGG'CCA Cuts at: 10 64 1489 1832 2330 2468 10 Size: 54 1425 343 498 138 4308 Fragments arranged by size: 4308 1425 498 343 138 54 MslI CAynn'nnrTG Cuts at: 518 1166 2292 2407 3003 3009 3159 4724 5083 Size: 648 1126 115 596 6 150 1565 359 Cuts at: 5083 5242 518 Size: 159 2042 Fragments arranged by size: 2042 1565 1126 648 596 359 159 150 115 6 MspA1I CmG'CkG Cuts at: 1606 3526 4182 4859 6007 6252 1606 Size: 1920 656 677 1148 245 2120 Fragments arranged by size: 2120 1920 1148 677 656 245 NaeI GCC'GGC Cuts at: 3818 3818 Size: 6766 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5275 Fragments arranged by size: 5275 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6766 NgoMIV G'CCGG_C Cuts at: 3816 3816 Size: 6766 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6766 NotI GC'GGCC_GC Cuts at: 4213 4213 Size: 6766 NspI r_CATG'y Cuts at: 1132 1229 4124 5669 1132 Size: 97 2895 1545 2229 Fragments arranged by size: 2895 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6766 PciI A'CATG_T Cuts at: 5665 5665 Size: 6766 PflMI CCAn_nnn'nTGG Cuts at: 1108 2464 2789 3244 1108 Size: 1356 325 455 4630 Fragments arranged by size: 4630 1356 455 325 PfoI T'CCnGG_A Cuts at: 2258 2258 Size: 6766 PmeI GTTT'AAAC Cuts at: 4070 4070 Size: 6766 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4835 4867 6348 6380 4835 Size: 32 1481 32 5221 Fragments arranged by size: 5221 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2203 2496 2719 1613 Size: 590 293 223 5660 Fragments arranged by size: 5660 590 293 223 PsiI TTA'TAA Cuts at: 4336 4336 Size: 6766 PstI C_TGCA'G Cuts at: 830 3043 3926 4118 830 Size: 2213 883 192 3478 Fragments arranged by size: 3478 2213 883 192 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6766 PvuII CAG'CTG Cuts at: 3526 3526 Size: 6766 RsrII CG'GwC_CG Cuts at: 3544 3544 Size: 6766 SacI G_AGCT'C Cuts at: 2586 2586 Size: 6766 SalI G'TCGA_C Cuts at: 1936 4108 1936 Size: 2172 4594 SbfI CC_TGCA'GG Cuts at: 4118 4118 Size: 6766 ScaI AGT'ACT Cuts at: 5014 6726 5014 Size: 1712 5054 SfcI C'TryA_G Cuts at: 826 1045 2089 2519 3039 3922 4114 5249 5930 Size: 219 1044 430 520 883 192 1135 681 Cuts at: 5930 6121 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1044 883 681 520 430 219 192 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6766 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6766 SmaI CCC'GGG Cuts at: 1872 4093 1872 Size: 2221 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3948 4870 5771 6033 820 Size: 197 926 2005 922 901 262 1553 Fragments arranged by size: 2005 1553 926 922 901 262 197 SnaBI TAC'GTA Cuts at: 493 4557 493 Size: 4064 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6766 SphI G_CATG'C Cuts at: 1132 4124 1132 Size: 2992 3774 SspI AAT'ATT Cuts at: 5 52 4690 5 Size: 47 4638 2081 Fragments arranged by size: 4638 2081 47 StuI AGG'CCT Cuts at: 1517 2453 1517 Size: 936 5830 StyI C'CwwG_G Cuts at: 513 1065 2004 2133 2783 2823 3571 4205 513 Size: 552 939 129 650 40 748 634 3074 Fragments arranged by size: 3074 939 748 650 634 552 129 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2419 2598 2603 3594 4793 4810 4963 Size: 1255 839 179 5 991 1199 17 153 Cuts at: 4963 5148 5593 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1199 991 839 445 185 179 153 17 5 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3210 3944 5012 Size: 274 80 33 51 1443 1233 734 1068 Cuts at: 5012 6724 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1233 1068 734 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2022 2054 2607 2639 3617 3649 6726 6758 2022 Size: 32 553 32 978 32 3077 32 2030 Fragments arranged by size: 3077 2030 978 553 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2127 3704 1316 Size: 811 1577 4378 Fragments arranged by size: 4378 1577 811 XbaI T'CTAG_A Cuts at: 4102 4102 Size: 6766 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 6766 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6766 XmaI C'CCGG_G Cuts at: 1870 4091 1870 Size: 2221 4545 XmnI GAAnn'nnTTC Cuts at: 4895 4895 Size: 6766 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4574 276 Size: 53 83 186 936 3040 2468 Fragments arranged by size: 3040 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI