(Circular) (Six-base) MAPSORT of: pFN21AB6161.seq Check: 5038 from: 1 to: 6502 pFN21AB6161 6502 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4312 278 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2068 2523 3845 1937 Size: 131 455 1322 4594 Fragments arranged by size: 4594 1322 455 131 Acc65I G'GTAC_C Cuts at: 3823 3823 Size: 6502 AclI AA'CG_TT Cuts at: 1563 3520 4629 5002 1563 Size: 1957 1109 373 3063 Fragments arranged by size: 3063 1957 1109 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4565 5949 6217 1632 Size: 2933 1384 268 1917 Fragments arranged by size: 2933 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6305 AflIII A'CryG_T Cuts at: 1203 5401 6378 1203 Size: 4198 977 1327 Fragments arranged by size: 4198 1327 977 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 5231 5231 Size: 6502 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6502 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2694 1184 Size: 1510 4992 AlwNI CAG_nnn'CTG Cuts at: 3119 4245 5817 3119 Size: 1126 1572 3804 Fragments arranged by size: 3804 1572 1126 ApaLI G'TGCA_C Cuts at: 4559 5715 4559 Size: 1156 5346 ApoI r'AATT_y Cuts at: 1493 2367 3080 3674 3811 4037 4189 6298 1493 Size: 874 713 594 137 226 152 2109 1697 Fragments arranged by size: 2109 1697 874 713 594 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 5056 160 Size: 872 4024 1606 Fragments arranged by size: 4024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 3507 3827 1870 Size: 73 1564 320 4545 Fragments arranged by size: 4545 1564 320 73 BamHI G'GATC_C Cuts at: 3832 4199 3832 Size: 367 6135 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 3823 5278 618 Size: 325 217 627 420 1616 1455 1842 Fragments arranged by size: 1842 1616 1455 627 420 325 217 BanII G_rGCy'C Cuts at: 1391 3821 1391 Size: 2430 4072 BbeI G_GCGC'C Cuts at: 2211 2211 Size: 6502 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3193 928 Size: 971 1294 4237 Fragments arranged by size: 4237 1294 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2058 2092 4693 4727 1903 Size: 34 121 34 2601 34 3678 Fragments arranged by size: 3678 2601 121 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4394 5604 4394 Size: 1210 5292 BclI T'GATC_A Cuts at: 1548 3370 1548 Size: 1822 4680 BfrBI ATG'CAT Cuts at: 2118 2984 2118 Size: 866 5636 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2205 5113 136 Size: 107 122 71 631 605 533 2908 1525 Fragments arranged by size: 2908 1525 631 605 533 122 107 71 BglII A'GATC_T Cuts at: 2650 6497 2650 Size: 3847 2655 BlpI GC'TnA_GC Cuts at: 3919 3919 Size: 6502 Bme1580I G_kGCm'C Cuts at: 1790 4563 5719 1790 Size: 2773 1156 2573 Fragments arranged by size: 2773 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6502 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4233 5191 442 Size: 416 53 83 399 24 2816 958 1753 Fragments arranged by size: 2816 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3092 1056 Size: 2036 4466 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3123 5162 1239 Size: 1884 2039 2579 Fragments arranged by size: 2579 2039 1884 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2989 4627 5492 5790 2989 Size: 1638 865 298 3701 Fragments arranged by size: 3701 1638 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5165 882 Size: 617 3666 2219 Fragments arranged by size: 3666 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4293 493 Size: 711 3089 2702 Fragments arranged by size: 3089 2702 711 BsaBI GATnn'nnATC Cuts at: 6496 6496 Size: 6502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2208 4309 4691 5326 Size: 53 83 186 936 675 2101 382 635 Cuts at: 5326 275 Size: 1451 Fragments arranged by size: 2101 1451 936 675 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4935 5333 5607 5754 6165 4935 Size: 398 274 147 411 5272 Fragments arranged by size: 5272 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2834 2864 3198 3228 1178 Size: 30 165 30 1431 30 334 30 4452 Fragments arranged by size: 4452 1431 334 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2676 3222 1186 Size: 1490 546 4466 Fragments arranged by size: 4466 1490 546 BseYI C'CCAG_C Cuts at: 3136 5705 6201 3136 Size: 2569 496 3437 Fragments arranged by size: 3437 2569 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3401 1163 Size: 573 1665 4264 Fragments arranged by size: 4264 1665 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3952 4713 5741 1540 Size: 275 186 1951 761 1028 2301 Fragments arranged by size: 2301 1951 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4563 4648 5719 4563 Size: 85 1071 5346 Fragments arranged by size: 5346 1071 85 BsmI GAATG_Cn' Cuts at: 2081 3180 4013 4106 2081 Size: 1099 833 93 4477 Fragments arranged by size: 4477 1099 833 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3821 4563 4648 5719 1391 Size: 399 2031 742 85 1071 2174 Fragments arranged by size: 2174 2031 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6165 6165 Size: 6502 BspHI T'CATG_A Cuts at: 4391 6121 4391 Size: 1730 4772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3448 3857 844 Size: 2604 409 3489 Fragments arranged by size: 3489 2604 409 BsrBI CCG'CTC Cuts at: 1394 1823 4389 1394 Size: 429 2566 3507 Fragments arranged by size: 3507 2566 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4997 5171 65 Size: 1183 3749 174 1396 Fragments arranged by size: 3749 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5146 5333 1700 Size: 110 3336 187 2869 Fragments arranged by size: 3336 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6502 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6502 BssSI C'ACGA_G Cuts at: 4562 5574 4562 Size: 1012 5490 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6502 BstBI TT'CG_AA Cuts at: 2101 5306 2101 Size: 3205 3297 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6502 BstYI r'GATC_y Cuts at: 1972 2450 2558 2650 3709 3832 4199 4584 4601 Size: 478 108 92 1059 123 367 385 17 Cuts at: 4601 6053 6139 6151 6497 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1059 478 385 367 346 123 108 92 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2535 2535 Size: 6502 BtgI C'CryG_G Cuts at: 513 1065 2004 2446 513 Size: 552 939 442 4569 Fragments arranged by size: 4569 939 552 442 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5763 Fragments arranged by size: 5763 683 56 BtsI GCAGTG_nn' Cuts at: 4018 4812 4832 4018 Size: 794 20 5688 Fragments arranged by size: 5688 794 20 ClaI AT'CG_AT Cuts at: 1554 2260 1554 Size: 706 5796 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6467 DraI TTT'AAA Cuts at: 2364 2763 2998 3806 2364 Size: 399 235 808 5060 Fragments arranged by size: 5060 808 399 235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5509 809 Size: 507 4193 1802 Fragments arranged by size: 4193 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3576 3949 4838 Size: 54 1425 50 275 18 1746 373 889 Cuts at: 4838 6467 8 Size: 1629 43 Fragments arranged by size: 1746 1629 1425 889 373 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3949 1537 Size: 275 2137 4090 Fragments arranged by size: 4090 2137 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3700 3767 4432 1353 Size: 2347 67 665 3423 Fragments arranged by size: 3423 2347 665 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5089 5463 5609 387 Size: 1064 138 3500 374 146 1280 Fragments arranged by size: 3500 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3123 4565 5162 5949 6217 1239 Size: 393 1491 1442 597 787 268 1524 Fragments arranged by size: 1524 1491 1442 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2307 3201 2307 Size: 894 5608 EcoRI G'AATT_C Cuts at: 3811 4189 3811 Size: 378 6124 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3324 3565 3324 Size: 241 6261 FspI TGC'GCA Cuts at: 5008 5008 Size: 6502 HaeII r_GCGC'y Cuts at: 1678 2211 2889 5649 1678 Size: 533 678 2760 2531 Fragments arranged by size: 2760 2531 678 533 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2834 2866 3452 3484 3490 Size: 32 135 32 1262 32 586 32 6 Cuts at: 3490 3522 5143 5175 5217 5249 1373 Size: 32 1621 32 42 32 2626 Fragments arranged by size: 2626 1621 1262 586 135 42 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 2019 2038 2069 2355 3846 4092 Size: 988 281 81 19 31 286 1491 246 Cuts at: 4092 669 Size: 3079 Fragments arranged by size: 3079 1491 988 286 281 246 81 31 19 HindIII A'AGCT_T Cuts at: 748 748 Size: 6502 HpaI GTT'AAC Cuts at: 2019 4092 2019 Size: 2073 4429 KasI G'GCGC_C Cuts at: 2207 2207 Size: 6502 KpnI G_GTAC'C Cuts at: 3827 3827 Size: 6502 MfeI C'AATT_G Cuts at: 2217 2598 3093 4101 2217 Size: 381 495 1008 4618 Fragments arranged by size: 4618 1008 495 381 MluI A'CGCG_T Cuts at: 6378 6378 Size: 6502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2311 2877 2981 5616 5800 1305 Size: 1006 566 104 2635 184 2007 Fragments arranged by size: 2635 2007 1006 566 184 104 MscI TGG'CCA Cuts at: 10 64 1489 1832 3578 10 Size: 54 1425 343 1746 2934 Fragments arranged by size: 2934 1746 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2816 4460 4819 4978 518 Size: 648 1650 1644 359 159 2042 Fragments arranged by size: 2042 1650 1644 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3918 4595 5743 5988 1606 Size: 2312 677 1148 245 2120 Fragments arranged by size: 2312 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2208 2208 Size: 6502 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5011 Fragments arranged by size: 5011 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6502 NheI G'CTAG_C Cuts at: 1052 3088 1052 Size: 2036 4466 NotI GC'GGCC_GC Cuts at: 3949 3949 Size: 6502 NsiI A_TGCA'T Cuts at: 2120 2986 2120 Size: 866 5636 NspI r_CATG'y Cuts at: 1132 1229 3860 5405 1132 Size: 97 2631 1545 2229 Fragments arranged by size: 2631 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6502 PciI A'CATG_T Cuts at: 5401 5401 Size: 6502 PflMI CCAn_nnn'nTGG Cuts at: 1108 2396 1108 Size: 1288 5214 PfoI T'CCnGG_A Cuts at: 3055 3055 Size: 6502 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4571 4603 6084 6116 4571 Size: 32 1481 32 4957 Fragments arranged by size: 4957 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 3237 4072 3237 Size: 835 5667 PspXI vC'TCGA_Gb Cuts at: 3507 3507 Size: 6502 PstI C_TGCA'G Cuts at: 830 3854 830 Size: 3024 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 SalI G'TCGA_C Cuts at: 1936 2067 3844 1936 Size: 131 1777 4594 Fragments arranged by size: 4594 1777 131 SbfI CC_TGCA'GG Cuts at: 3854 3854 Size: 6502 ScaI AGT'ACT Cuts at: 4750 6462 4750 Size: 1712 4790 SfcI C'TryA_G Cuts at: 826 1045 2094 3404 3721 3850 4985 5666 5857 Size: 219 1049 1310 317 129 1135 681 191 Cuts at: 5857 826 Size: 1471 Fragments arranged by size: 1471 1310 1135 1049 681 317 219 191 129 SfoI GGC'GCC Cuts at: 2209 2209 Size: 6502 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6502 SmaI CCC'GGG Cuts at: 1872 3829 1872 Size: 1957 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2968 3507 4606 5507 5769 820 Size: 197 926 1025 539 1099 901 262 1553 Fragments arranged by size: 1553 1099 1025 926 901 539 262 197 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6502 SphI G_CATG'C Cuts at: 1132 3860 1132 Size: 2728 3774 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6502 StyI C'CwwG_G Cuts at: 513 1065 2004 2395 3941 513 Size: 552 939 391 1546 3074 Fragments arranged by size: 3074 1546 939 552 391 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2586 3333 3347 4529 4546 4699 4884 Size: 1255 1006 747 14 1182 17 153 185 Cuts at: 4884 5329 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1182 1006 747 445 185 153 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4748 6460 96 Size: 274 80 33 51 1443 2771 1712 138 Fragments arranged by size: 2771 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6462 6494 6462 Size: 32 6470 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6502 XbaI T'CTAG_A Cuts at: 2482 3838 2482 Size: 1356 5146 XhoI C'TCGA_G Cuts at: 1943 3507 1943 Size: 1564 4938 XmaI C'CCGG_G Cuts at: 1870 3827 1870 Size: 1957 4545 XmnI GAAnn'nnTTC Cuts at: 4631 4631 Size: 6502 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4310 276 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI