Restriction map for
pFN21AB6161
(Linear) (Six-base) MAPSORT of: KIBB6161.seq Check: 6976 from: 1 to: 1797
KIBB6161.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 63 518 1797
Size: 63 455 1279
Fragments arranged by size:
1279 455 63
AclI AA'CG_TT
Cuts at: 0 1515 1797
Size: 1515 282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 689 1797
Size: 689 1108
AlwNI CAG_nnn'CTG
Cuts at: 0 1114 1797
Size: 1114 683
ApoI r'AATT_y
Cuts at: 0 362 1075 1669 1797
Size: 362 713 594 128
Fragments arranged by size:
713 594 362 128
AvaI C'yCGr_G
Cuts at: 0 1502 1797
Size: 1502 295
BanI G'GyrC_C
Cuts at: 0 202 1797
Size: 202 1595
BbeI G_GCGC'C
Cuts at: 0 206 1797
Size: 206 1591
BbsI GAAGACnn'nnnn_
Cuts at: 0 1188 1797
Size: 1188 609
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 53 87 1797
Size: 53 34 1710
Fragments arranged by size:
1710 53 34
BclI T'GATC_A
Cuts at: 0 1365 1797
Size: 1365 432
BfrBI ATG'CAT
Cuts at: 0 113 979 1797
Size: 113 866 818
Fragments arranged by size:
866 818 113
BglI GCCn_nnn'nGGC
Cuts at: 0 200 1797
Size: 200 1597
BglII A'GATC_T
Cuts at: 0 645 1797
Size: 645 1152
BmtI G_CTAG'C
Cuts at: 0 1087 1797
Size: 1087 710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1118 1797
Size: 1118 679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1797
Size: 984 813
BsaHI Gr'CG_yC
Cuts at: 0 203 1797
Size: 203 1594
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 829 859 1193 1223 1797
Size: 829 30 334 30 574
Fragments arranged by size:
829 574 334 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 671 1217 1797
Size: 671 546 580
Fragments arranged by size:
671 580 546
BseYI C'CCAG_C
Cuts at: 0 1131 1797
Size: 1131 666
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1396 1797
Size: 1396 401
BsmI GAATG_Cn'
Cuts at: 0 76 1175 1797
Size: 76 1099 622
Fragments arranged by size:
1099 622 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1443 1797
Size: 1443 354
BstBI TT'CG_AA
Cuts at: 0 96 1797
Size: 96 1701
BstYI r'GATC_y
Cuts at: 0 445 553 645 1704 1797
Size: 445 108 92 1059 93
Fragments arranged by size:
1059 445 108 93 92
Bsu36I CC'TnA_GG
Cuts at: 0 530 1797
Size: 530 1267
BtgI C'CryG_G
Cuts at: 0 441 1797
Size: 441 1356
ClaI AT'CG_AT
Cuts at: 0 255 1797
Size: 255 1542
DraI TTT'AAA
Cuts at: 0 359 758 993 1797
Size: 359 399 235 804
Fragments arranged by size:
804 399 359 235
EaeI y'GGCC_r
Cuts at: 0 1571 1797
Size: 1571 226
EarI CTCTTCn'nnn_
Cuts at: 0 1695 1762 1797
Size: 1695 67 35
Fragments arranged by size:
1695 67 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1118 1797
Size: 1118 679
EcoNI CCTnn'n_nnAGG
Cuts at: 0 302 1196 1797
Size: 302 894 601
Fragments arranged by size:
894 601 302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1319 1560 1797
Size: 1319 241 237
Fragments arranged by size:
1319 241 237
HaeII r_GCGC'y
Cuts at: 0 206 884 1797
Size: 206 678 913
Fragments arranged by size:
913 678 206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 829 861 1447 1479 1485 1517 1797
Size: 829 32 586 32 6 32 280
Fragments arranged by size:
829 586 280 32 32 32 6
HincII GTy'rAC
Cuts at: 0 14 33 64 350 1797
Size: 14 19 31 286 1447
Fragments arranged by size:
1447 286 31 19 14
HpaI GTT'AAC
Cuts at: 0 14 1797
Size: 14 1783
KasI G'GCGC_C
Cuts at: 0 202 1797
Size: 202 1595
MfeI C'AATT_G
Cuts at: 0 212 593 1088 1797
Size: 212 381 495 709
Fragments arranged by size:
709 495 381 212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 872 976 1797
Size: 306 566 104 821
Fragments arranged by size:
821 566 306 104
MscI TGG'CCA
Cuts at: 0 1573 1797
Size: 1573 224
MslI CAynn'nnrTG
Cuts at: 0 811 1797
Size: 811 986
NarI GG'CG_CC
Cuts at: 0 203 1797
Size: 203 1594
NheI G'CTAG_C
Cuts at: 0 1083 1797
Size: 1083 714
NsiI A_TGCA'T
Cuts at: 0 115 981 1797
Size: 115 866 816
Fragments arranged by size:
866 816 115
PflMI CCAn_nnn'nTGG
Cuts at: 0 391 1797
Size: 391 1406
PfoI T'CCnGG_A
Cuts at: 0 1050 1797
Size: 1050 747
PsiI TTA'TAA
Cuts at: 0 1232 1797
Size: 1232 565
PspXI vC'TCGA_Gb
Cuts at: 0 1502 1797
Size: 1502 295
SalI G'TCGA_C
Cuts at: 0 62 1797
Size: 62 1735
SfcI C'TryA_G
Cuts at: 0 89 1399 1716 1797
Size: 89 1310 317 81
Fragments arranged by size:
1310 317 89 81
SfoI GGC'GCC
Cuts at: 0 204 1797
Size: 204 1593
SmlI C'TyrA_G
Cuts at: 0 963 1502 1797
Size: 963 539 295
Fragments arranged by size:
963 539 295
StyI C'CwwG_G
Cuts at: 0 390 1797
Size: 390 1407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 581 1328 1342 1797
Size: 581 747 14 455
Fragments arranged by size:
747 581 455 14
XbaI T'CTAG_A
Cuts at: 0 477 1797
Size: 477 1320
XhoI C'TCGA_G
Cuts at: 0 1502 1797
Size: 1502 295
Enzymes that do cut:
AccI AclI AlfI AlwNI ApoI AvaI BanI BbeI
BbsI BcgI BclI BfrBI BglI BglII BmtI BpmI
BpuEI BsaHI BsaXI BseRI BseYI BsgI BsmI BspMI
BstBI BstYI Bsu36I BtgI ClaI DraI EaeI EarI
Eco57MI EcoNI FalI HaeII Hin4I HincII HpaI KasI
MfeI MmeI MscI MslI NarI NheI NsiI PflMI
PfoI PsiI PspXI SalI SfcI SfoI SmlI StyI
TaqII XbaI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbvCI BciVI BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BsaI BsaAI BsaBI BsaWI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I BtgZI BtsI CspCI DraIII DrdI EagI EciI
EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HindIII
KpnI MluI MspA1I NaeI NcoI NdeI NgoMIV NotI
NruI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XcmI XmaI XmnI
ZraI