Restriction map for
pFN21AE0783
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MboII MluI
MseI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI TaqI TfiI
Tru9I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 347 CGCG CG^CG
AccIII 707 TCCGGA T^CCGGA
AcyI 307 GACGCC G[GA]^CG[CT]C
Alw44I 55 GTGCAC G^TGCAC
AocI 466 CCTGAGG CC^T[ACGT]AGG
ApaLI 55 GTGCAC G^TGCAC
AspI 85 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 809 TGGCCA TGG^CCA
BanII 944 GAGCCC G[GA]GC[CT]^C
BcgI 438 CGAGGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 307 GACGCC G[GA]^CG[CT]C
BsaI 592 GAGACC GGTCTC(1/5)
BseAI 707 TCCGGA T^CCGGA
BsmAI 592 GAGAC GTCTC(1/5)
BspEI 707 TCCGGA T^CCGGA
BspHI 269 TCATGA T^CATGA
BstAPI 224 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 347 CGCG CG^CG
BstXI 425 CCAACGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 466 CCTGAGG CC^T[ACGT]AGG
CfoI 348 GCGC GCG^C
Eco81I 466 CCTGAGG CC^T[ACGT]AGG
HgaI 307 GACGC GACGC(5/10)
HhaI 348 GCGC GCG^C
Hin1I 307 GACGCC G[GA]^CG[CT]C
HinP1I 348 GCGC G^CGC
MaeII 395 ACGT A^CGT
MfeI 338 CAATTG C^AATTG
MluNI 809 TGGCCA TGG^CCA
MroI 707 TCCGGA T^CCGGA
MscI 809 TGGCCA TGG^CCA
MunI 338 CAATTG C^AATTG
MvnI 347 CGCG CG^CG
NcoI 105 CCATGG C^CATGG
PflFI 85 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 372 CTGCAG CTGCA^G
RcaI 269 TCATGA T^CATGA
SbfI 371 CCTGCAGG CCTGCA^GG
SfaNI 127 GATGC GCATC(5/9)
SfcI 372 CTGCAG C^T[GA][CT]AG
SmaI 277 CCCGGG CCC^GGG
SmlI 117 CTCAAG C^T[CT][GA]AG
Sse8387I 371 CCTGCAGG CCTGCA^GG
StuI 473 AGGCCT AGG^CCT
Tsp509I 339 AATT ^AATT
Tth111I 85 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 37 CCACGGCTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 277 CCCGGG C^CCGGG
XmaI 277 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 1020 GTAC GT^AC
1050 GTAC
AspHI 55 GTGCAC G[AT]GC[AT]^C
973 GAGCAC
BanI 843 GGCACC G^G[CT][GA]CC
926 GGCACC
BglI 188 GCCATGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
369 GCCCTGCAGGC
BpmI 142 CTGGAG CTGGAG(16/14)
609 CTGGAG
BsaWI 707 TCCGGA [AT]^CCGG[AT]
739 ACCGGA
BsiHKAI 55 GTGCAC G[AT]GC[AT]^C
973 GAGCAC
BstEII 315 GGTCACC G^GT[ACGT]ACC
903 GGTGACC
BstPI 315 GGTCACC G^GT[ACGT]ACC
903 GGTGACC
DrdI 307 GACGCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
853 GACAGAAAGGTC
EaeI 434 TGGCCG [CT]^GGCC[GA]
809 TGGCCA
EcoO65I 315 GGTCACC G^GT[ACGT]ACC
903 GGTGACC
EcoT14I 105 CCATGG C^C[AT][AT]GG
548 CCAAGG
FauI 388 CCCGC CCCGC(4/6)
596 CCCGC
HinfI 145 GAGTC G^A[ACGT]TC
961 GAGTC
PflMI 234 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
978 CCAGGGCTTGG
PleI 145 GAGTC GAGTC(4/5)
961 GAGTC
PpuMI 89 GGGTCCC [GA]G^G[AT]CC[CT]
860 AGGTCCC
RsaI 1020 GTAC GT^AC
1050 GTAC
StyI 105 CCATGG C^C[AT][AT]GG
548 CCAAGG
Van91I 234 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
978 CCAGGGCTTGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaI
AvaII BamHI BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeIII MboI MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI TseI
Tsp45I TspRI XhoII