Restriction map for
pFN21AE0783
(Linear) (Six-base) MAPSORT of: KIEE0783.seq Check: 6026 from: 1 to: 1056
KIEE0783.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 373 1056
Size: 373 683
AlwNI CAG_nnn'CTG
Cuts at: 0 156 230 733 994 1056
Size: 156 74 503 261 62
Fragments arranged by size:
503 261 156 74 62
ApaLI G'TGCA_C
Cuts at: 0 55 1056
Size: 55 1001
AvaI C'yCGr_G
Cuts at: 0 94 277 631 777 1056
Size: 94 183 354 146 279
Fragments arranged by size:
354 279 183 146 94
BamHI G'GATC_C
Cuts at: 0 160 384 768 798 1056
Size: 160 224 384 30 258
Fragments arranged by size:
384 258 224 160 30
BanI G'GyrC_C
Cuts at: 0 843 926 1056
Size: 843 83 130
Fragments arranged by size:
843 130 83
BanII G_rGCy'C
Cuts at: 0 948 1056
Size: 948 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 427 461 1056
Size: 427 34 595
Fragments arranged by size:
595 427 34
BglI GCCn_nnn'nGGC
Cuts at: 0 194 375 1056
Size: 194 181 681
Fragments arranged by size:
681 194 181
Bme1580I G_kGCm'C
Cuts at: 0 59 1056
Size: 59 997
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 1056
Size: 166 890
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 492 1056
Size: 492 564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 630 1056
Size: 163 467 426
Fragments arranged by size:
467 426 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 1056
Size: 102 954
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 1056
Size: 586 470
BsaHI Gr'CG_yC
Cuts at: 0 308 1056
Size: 308 748
BsaWI w'CCGG_w
Cuts at: 0 707 739 1056
Size: 707 32 317
Fragments arranged by size:
707 317 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 190 220 1056
Size: 190 30 836
Fragments arranged by size:
836 190 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 627 1030 1056
Size: 484 143 403 26
Fragments arranged by size:
484 403 143 26
BsiHKAI G_wGCw'C
Cuts at: 0 59 977 1056
Size: 59 918 79
Fragments arranged by size:
918 79 59
Bsp1286I G_dGCh'C
Cuts at: 0 59 948 977 1056
Size: 59 889 29 79
Fragments arranged by size:
889 79 59 29
BspEI T'CCGG_A
Cuts at: 0 707 1056
Size: 707 349
BspHI T'CATG_A
Cuts at: 0 269 1056
Size: 269 787
BstAPI GCAn_nnn'nTGC
Cuts at: 0 230 1056
Size: 230 826
BstEII G'GTnAC_C
Cuts at: 0 315 903 1056
Size: 315 588 153
Fragments arranged by size:
588 315 153
BstXI CCAn_nnnn'nTGG
Cuts at: 0 432 1056
Size: 432 624
BstYI r'GATC_y
Cuts at: 0 160 384 408 712 768 798 1056
Size: 160 224 24 304 56 30 258
Fragments arranged by size:
304 258 224 160 56 30 24
Bsu36I CC'TnA_GG
Cuts at: 0 467 1056
Size: 467 589
BtgI C'CryG_G
Cuts at: 0 37 105 716 1056
Size: 37 68 611 340
Fragments arranged by size:
611 340 68 37
BtsI GCAGTG_nn'
Cuts at: 0 437 1056
Size: 437 619
DrdI GACnn_nn'nnGTC
Cuts at: 0 313 859 1056
Size: 313 546 197
Fragments arranged by size:
546 313 197
EaeI y'GGCC_r
Cuts at: 0 434 809 1056
Size: 434 375 247
Fragments arranged by size:
434 375 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 630 1056
Size: 163 467 426
Fragments arranged by size:
467 426 163
EcoO109I rG'GnC_Cy
Cuts at: 0 90 730 861 1056
Size: 90 640 131 195
Fragments arranged by size:
640 195 131 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 152 184 760 792 1056
Size: 152 32 576 32 264
Fragments arranged by size:
576 264 152 32 32
MfeI C'AATT_G
Cuts at: 0 338 1056
Size: 338 718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1056
Size: 449 607
MscI TGG'CCA
Cuts at: 0 811 1056
Size: 811 245
MslI CAynn'nnrTG
Cuts at: 0 64 188 901 1056
Size: 64 124 713 155
Fragments arranged by size:
713 155 124 64
MspA1I CmG'CkG
Cuts at: 0 75 227 893 1056
Size: 75 152 666 163
Fragments arranged by size:
666 163 152 75
NcoI C'CATG_G
Cuts at: 0 105 1056
Size: 105 951
PasI CC'CwG_GG
Cuts at: 0 733 910 1045 1056
Size: 733 177 135 11
Fragments arranged by size:
733 177 135 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 984 1056
Size: 240 744 72
Fragments arranged by size:
744 240 72
PfoI T'CCnGG_A
Cuts at: 0 1010 1056
Size: 1010 46
PpuMI rG'GwC_Cy
Cuts at: 0 90 861 1056
Size: 90 771 195
Fragments arranged by size:
771 195 90
PstI C_TGCA'G
Cuts at: 0 376 1056
Size: 376 680
PvuII CAG'CTG
Cuts at: 0 75 227 893 1056
Size: 75 152 666 163
Fragments arranged by size:
666 163 152 75
SanDI GG'GwC_CC
Cuts at: 0 90 1056
Size: 90 966
SbfI CC_TGCA'GG
Cuts at: 0 376 1056
Size: 376 680
SfcI C'TryA_G
Cuts at: 0 372 1056
Size: 372 684
SmaI CCC'GGG
Cuts at: 0 279 1056
Size: 279 777
SmlI C'TyrA_G
Cuts at: 0 117 1056
Size: 117 939
StuI AGG'CCT
Cuts at: 0 475 1056
Size: 475 581
StyI C'CwwG_G
Cuts at: 0 105 548 1056
Size: 105 443 508
Fragments arranged by size:
508 443 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 876 1056
Size: 876 180
Tth111I GACn'n_nGTC
Cuts at: 0 88 1056
Size: 88 968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 44 1056
Size: 44 1012
XmaI C'CCGG_G
Cuts at: 0 277 1056
Size: 277 779
Enzymes that do cut:
AlfI AlwNI ApaLI AvaI BamHI BanI BanII BcgI
BglI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspEI BspHI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
Eco57MI EcoO109I Hin4I MfeI MmeI MscI MslI MspA1I
NcoI PasI PflMI PfoI PpuMI PstI PvuII SanDI
SbfI SfcI SmaI SmlI StuI StyI TaqII Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaAI
BsaBI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
BtgZI ClaI CspCI DraI DraIII EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI XbaI XhoI
XmnI ZraI