Restriction map for
pFN21AB5975
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspHI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp45I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 568 GTCTAC GT^[AC][GT]AC
AccII 18 CGCG CG^CG
AccIII 131 TCCGGA T^CCGGA
AcsI 1234 GAATTT [GA]^AATT[CT]
AfaI 1146 GTAC GT^AC
AflIII 274 ACATGT A^C[GA][CT]GT
AocI 1445 CCTCAGG CC^T[ACGT]AGG
ApoI 1234 GAATTT [GA]^AATT[CT]
AspHI 1015 GAGCTC G[AT]GC[AT]^C
AvaII 1307 GGACC G^G[AT]CC
AvrII 420 CCTAGG C^CTAGG
BalI 320 TGGCCA TGG^CCA
BanI 897 GGCACC G^G[CT][GA]CC
BbsI 1416 GTCTTC GAAGAC(2/6)
BbvCI 4 GCTGAGG CCTCAGC(-5/-2)
BcnI 62 CCCGG CC^[GC]GG
BglII 325 AGATCT A^GATCT
BlnI 420 CCTAGG C^CTAGG
BpuAI 1416 GTCTTC GAAGAC(2/6)
BsaWI 131 TCCGGA [AT]^CCGG[AT]
BseAI 131 TCCGGA T^CCGGA
BseRI 7 GAGGAG GAGGAG(10/8)
BsgI 278 GTGCAG GTGCAG(16/14)
BsiHKAI 1015 GAGCTC G[AT]GC[AT]^C
BsmFI 749 GGGAC GGGAC(10/14)
Bsp1407I 1145 TGTACA T^GTACA
BspEI 131 TCCGGA T^CCGGA
BspLU11I 274 ACATGT A^CATGT
BsrGI 1145 TGTACA T^GTACA
BstF5I 1463 CATCC GGATG(2/0)
BstUI 18 CGCG CG^CG
BstXI 887 CCAACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1445 CCTCAGG CC^T[ACGT]AGG
DsaI 744 CCACGG C^C[GA][CT]GG
Eco81I 1445 CCTCAGG CC^T[ACGT]AGG
EcoT14I 420 CCTAGG C^C[AT][AT]GG
EcoT22I 959 ATGCAT ATGCA^T
FokI 1463 CATCC GGATG(9/13)
HgaI 1437 GACGC GACGC(5/10)
MaeIII 1109 GTAAC ^GT[ACGT]AC
MluNI 320 TGGCCA TGG^CCA
MroI 131 TCCGGA T^CCGGA
MscI 320 TGGCCA TGG^CCA
MslI 825 CATTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 18 CGCG CG^CG
NciI 62 CCCGG CC^[GC]GG
NruI 17 TCGCGA TCG^CGA
NsiI 959 ATGCAT ATGCA^T
PflMI 531 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 1146 GTAC GT^AC
SacI 1015 GAGCTC GAGCT^C
SspBI 1145 TGTACA T^GTACA
StyI 420 CCTAGG C^C[AT][AT]GG
TaqI 905 TCGA T^CGA
TthHB8I 905 TCGA T^CGA
Van91I 531 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwI 528 GATCC GGATC(4/5)
1324 GATCC
Asp700I 169 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
444 GAAGCAGTTC
BanII 1015 GAGCTC G[GA]GC[CT]^C
1386 GGGCTC
BglI 148 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
712 GCCATCTTGGC
BmyI 1015 GAGCTC G[AGT]GC[ACT]^C
1386 GGGCTC
Bsp1286I 1015 GAGCTC G[AGT]GC[ACT]^C
1386 GGGCTC
BspMI 1362 ACCTGC ACCTGC(4/8)
315 GCAGGT
BsrDI 677 GCAATG GCAATG(2/0)
1411 GCAATG
EarI 265 CTCTTC CTCTTC(1/4)
1487 CTCTTC
EcoNI 309 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1209 CCTACTCAAGG
HapII 63 CCGG C^CGG
132 CCGG
HpaII 63 CCGG C^CGG
132 CCGG
HphI 592 TCACC GGTGA(8/7)
767 TCACC
Ksp632I 265 CTCTTC CTCTTC(1/4)
1487 CTCTTC
MseI 491 TTAA T^TAA
1319 TTAA
MspA1I 870 CAGCTG C[AC]G^C[GT]G
1424 CAGCTG
MspI 63 CCGG C^CGG
132 CCGG
NlaIV 897 GGCACC GG[ACGT]^[ACGT]CC
1497 GGAGCC
NspI 274 ACATGT [GA]CATG^[CT]
1022 ACATGC
PvuII 870 CAGCTG CAG^CTG
1424 CAGCTG
SfaNI 735 GATGC GCATC(5/9)
961 GCATC
SmlI 310 CTCAAG C^T[CT][GA]AG
1213 CTCAAG
Tru9I 491 TTAA T^TAA
1319 TTAA
XmnI 169 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
444 GAAGCAGTTC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwNI BbvI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstNI BstYI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI HaeIII HinfI ItaI MaeI
MboI MboII MflI MnlI MvaI
MwoI NdeII NlaIII PleI Sau3AI
Sau96I ScrFI TfiI TseI Tsp509I
TspRI XhoII