(Circular) (Six-base) MAPSORT of: pFN21AB5975.seq Check: 8777 from: 1 to: 6262 pFN21AB5975 6262 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2311 2311 Size: 6262 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2574 3605 1937 Size: 637 1031 4594 Fragments arranged by size: 4594 1031 637 Acc65I G'GTAC_C Cuts at: 3583 3583 Size: 6262 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2468 3408 4325 5709 5977 1632 Size: 836 940 917 1384 268 1917 Fragments arranged by size: 1917 1384 940 917 836 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 2279 5161 6138 1203 Size: 1076 2882 977 1327 Fragments arranged by size: 2882 1327 1076 977 AgeI A'CCGG_T Cuts at: 5093 5093 Size: 6262 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6262 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2756 2979 1184 Size: 1572 223 4467 Fragments arranged by size: 4467 1572 223 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2023 2055 2023 Size: 32 6230 AlwNI CAG_nnn'CTG Cuts at: 3388 3431 3458 3512 4005 5577 3388 Size: 43 27 54 493 1572 4073 Fragments arranged by size: 4073 1572 493 54 43 27 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 3239 3571 3797 3949 6058 1493 Size: 1746 332 226 152 2109 1697 Fragments arranged by size: 2109 1746 1697 332 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 3587 1870 Size: 73 1644 4545 Fragments arranged by size: 4545 1644 73 AvrII C'CTAG_G Cuts at: 2425 2425 Size: 6262 BamHI G'GATC_C Cuts at: 3592 3959 3592 Size: 367 5895 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2902 3583 5038 618 Size: 325 217 627 1115 681 1455 1842 Fragments arranged by size: 1842 1455 1115 681 627 325 217 BanII G_rGCy'C Cuts at: 1391 3024 3395 3581 1391 Size: 1633 371 186 4072 Fragments arranged by size: 4072 1633 371 186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3414 928 Size: 971 1515 3776 Fragments arranged by size: 3776 1515 971 BbvCI CC'TCA_GC Cuts at: 2010 2010 Size: 6262 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4453 4487 1903 Size: 34 2516 34 3678 Fragments arranged by size: 3678 2516 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 1548 Size: 6262 BfrBI ATG'CAT Cuts at: 2966 2966 Size: 6262 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2159 2723 4873 Size: 107 122 71 631 605 487 564 2150 Cuts at: 4873 136 Size: 1525 Fragments arranged by size: 2150 1525 631 605 564 487 122 107 71 BglII A'GATC_T Cuts at: 2330 6257 2330 Size: 3927 2335 BlpI GC'TnA_GC Cuts at: 3679 3679 Size: 6262 Bme1580I G_kGCm'C Cuts at: 1790 4323 5479 1790 Size: 2533 1156 2573 Fragments arranged by size: 2573 2533 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6262 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3466 3993 4951 Size: 416 53 83 399 24 2049 527 958 Cuts at: 4951 442 Size: 1753 Fragments arranged by size: 2049 1753 958 527 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3302 3302 Size: 6262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2067 2564 3272 3480 3521 4922 1239 Size: 828 497 708 208 41 1401 2579 Fragments arranged by size: 2579 1401 828 708 497 208 41 Bpu10I CC'TnA_GC Cuts at: 2010 2010 Size: 6262 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2300 3203 4387 5252 5550 2300 Size: 903 1184 865 298 3012 Fragments arranged by size: 3012 1184 903 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4925 882 Size: 617 3426 2219 Fragments arranged by size: 3426 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4053 493 Size: 711 2849 2702 Fragments arranged by size: 2849 2702 711 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4069 4451 5086 275 Size: 53 83 186 936 2536 382 635 1451 Fragments arranged by size: 2536 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2136 4695 5093 5367 5514 5925 2136 Size: 2559 398 274 147 411 2473 Fragments arranged by size: 2559 2473 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6037 Fragments arranged by size: 6037 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 1186 Size: 841 5421 BseYI C'CCAG_C Cuts at: 2524 5465 5961 2524 Size: 2941 496 2825 Fragments arranged by size: 2941 2825 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2304 1163 Size: 573 568 5121 Fragments arranged by size: 5121 573 568 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3712 4473 5501 1540 Size: 275 186 1711 761 1028 2301 Fragments arranged by size: 2301 1711 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3024 4323 4408 5479 3024 Size: 1299 85 1071 3807 Fragments arranged by size: 3807 1299 1071 85 BsmI GAATG_Cn' Cuts at: 3773 3866 3773 Size: 93 6169 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3024 3395 3581 4323 4408 5479 1391 Size: 399 1234 371 186 742 85 1071 2174 Fragments arranged by size: 2174 1234 1071 742 399 371 186 85 BspEI T'CCGG_A Cuts at: 2136 5925 2136 Size: 3789 2473 BspHI T'CATG_A Cuts at: 4151 5881 4151 Size: 1730 4532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2311 3376 3617 844 Size: 1467 1065 241 3489 Fragments arranged by size: 3489 1467 1065 241 BsrBI CCG'CTC Cuts at: 1394 1823 4149 1394 Size: 429 2326 3507 Fragments arranged by size: 3507 2326 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2689 3423 4757 4931 65 Size: 1183 1441 734 1334 174 1396 Fragments arranged by size: 1441 1396 1334 1183 734 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4906 5093 1700 Size: 110 3096 187 2869 Fragments arranged by size: 3096 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3150 96 Size: 3054 3208 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6262 BssSI C'ACGA_G Cuts at: 4322 5334 4322 Size: 1012 5250 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6262 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6262 BstXI CCAn_nnnn'nTGG Cuts at: 2899 2899 Size: 6262 BstYI r'GATC_y Cuts at: 1972 2330 2532 3328 3592 3959 4344 4361 5813 Size: 358 202 796 264 367 385 17 1452 Cuts at: 5813 5899 5911 6257 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 796 385 367 358 346 264 202 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3451 3451 Size: 6262 BtgI C'CryG_G Cuts at: 513 1065 2004 2749 513 Size: 552 939 745 4026 Fragments arranged by size: 4026 939 745 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5523 Fragments arranged by size: 5523 683 56 BtsI GCAGTG_nn' Cuts at: 2592 3045 3778 4572 4592 2592 Size: 453 733 794 20 4262 Fragments arranged by size: 4262 794 733 453 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6227 DraI TTT'AAA Cuts at: 3566 3566 Size: 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5269 809 Size: 507 3953 1802 Fragments arranged by size: 3953 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 2868 2991 Size: 54 1425 50 275 18 495 543 123 Cuts at: 2991 3709 4598 6227 8 Size: 718 889 1629 43 Fragments arranged by size: 1629 1425 889 718 543 495 275 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3709 1537 Size: 275 1897 4090 Fragments arranged by size: 4090 1897 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2276 3498 4192 1353 Size: 923 1222 694 3423 Fragments arranged by size: 3423 1222 923 694 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4849 5223 5369 387 Size: 1064 138 3260 374 146 1280 Fragments arranged by size: 3260 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3022 3022 Size: 6262 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2067 2468 2564 3272 3408 3480 3521 Size: 393 435 401 96 708 136 72 41 Cuts at: 3521 4325 4922 5709 5977 1239 Size: 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 708 597 435 401 393 268 136 96 72 41 EcoNI CCTnn'n_nnAGG Cuts at: 2318 3218 2318 Size: 900 5362 EcoRI G'AATT_C Cuts at: 3571 3949 3571 Size: 378 5884 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2195 2554 2737 2195 Size: 359 183 5720 Fragments arranged by size: 5720 359 183 FspI TGC'GCA Cuts at: 4768 4768 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 5409 1678 Size: 3731 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2017 2049 3215 3247 3320 Size: 32 135 32 445 32 1166 32 73 Cuts at: 3320 3352 4903 4935 4977 5009 1373 Size: 32 1551 32 42 32 2626 Fragments arranged by size: 2626 1551 1166 445 135 73 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3606 3852 669 Size: 988 281 1668 246 3079 Fragments arranged by size: 3079 1668 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6262 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KpnI G_GTAC'C Cuts at: 3587 3587 Size: 6262 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3554 5376 5560 1305 Size: 2249 1822 184 2007 Fragments arranged by size: 2249 2007 1822 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 10 Size: 54 1425 343 495 3945 Fragments arranged by size: 3945 1425 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2834 4220 4579 4738 518 Size: 648 1668 1386 359 159 2042 Fragments arranged by size: 2042 1668 1386 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2877 3431 3678 4355 5503 5748 1606 Size: 1271 554 247 677 1148 245 2120 Fragments arranged by size: 2120 1271 1148 677 554 247 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4771 Fragments arranged by size: 4771 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NruI TCG'CGA Cuts at: 2024 2024 Size: 6262 NsiI A_TGCA'T Cuts at: 2968 2968 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 2283 3031 3620 5165 1132 Size: 97 1054 748 589 1545 2229 Fragments arranged by size: 2229 1545 1054 748 589 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6262 PciI A'CATG_T Cuts at: 2279 5161 2279 Size: 2882 3380 PflMI CCAn_nnn'nTGG Cuts at: 1108 2542 1108 Size: 1434 4828 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4331 4363 5844 5876 4331 Size: 32 1481 32 4717 Fragments arranged by size: 4717 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6262 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PstI C_TGCA'G Cuts at: 830 3614 830 Size: 2784 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 PvuII CAG'CTG Cuts at: 2877 3431 2877 Size: 554 5708 SacI G_AGCT'C Cuts at: 3024 3024 Size: 6262 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SfcI C'TryA_G Cuts at: 826 1045 3610 4745 5426 5617 826 Size: 219 2565 1135 681 191 1471 Fragments arranged by size: 2565 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 3589 1872 Size: 1717 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2315 3218 4366 5267 5529 820 Size: 197 926 372 903 1148 901 262 1553 Fragments arranged by size: 1553 1148 926 903 901 372 262 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 3620 1132 Size: 2488 3774 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6262 StyI C'CwwG_G Cuts at: 513 1065 2004 2425 3701 513 Size: 552 939 421 1276 3074 Fragments arranged by size: 3074 1276 939 552 421 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4289 4306 4459 4644 5089 325 Size: 1255 2709 17 153 185 445 1498 Fragments arranged by size: 2709 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3150 4508 6220 Size: 274 80 33 51 1443 1173 1358 1712 Cuts at: 6220 96 Size: 138 Fragments arranged by size: 1712 1443 1358 1173 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6222 6254 6222 Size: 32 6230 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6262 XbaI T'CTAG_A Cuts at: 3598 3598 Size: 6262 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 3587 1870 Size: 1717 4545 XmnI GAAnn'nnTTC Cuts at: 2178 2453 4391 2178 Size: 275 1938 4049 Fragments arranged by size: 4049 1938 275 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI