(Circular) (Six-base) MAPSORT of: pFN21AB5894.seq Check: 9192 from: 1 to: 6277 pFN21AB5894 6277 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3620 1937 Size: 1683 4594 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2681 4340 5724 5992 1632 Size: 1049 1659 1384 268 1917 Fragments arranged by size: 1917 1659 1384 1049 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 2134 5176 6153 1203 Size: 931 3042 977 1327 Fragments arranged by size: 3042 1327 977 931 AgeI A'CCGG_T Cuts at: 5108 5108 Size: 6277 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 2054 1166 Size: 888 5389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6277 AlwNI CAG_nnn'CTG Cuts at: 2704 3572 4020 5592 2704 Size: 868 448 1572 3389 Fragments arranged by size: 3389 1572 868 448 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 2630 3497 3551 3586 3812 3964 6073 1493 Size: 1137 867 54 35 226 152 2109 1697 Fragments arranged by size: 2109 1697 1137 867 226 152 54 35 AseI AT'TA_AT Cuts at: 160 1032 2823 4831 160 Size: 872 1791 2008 1606 Fragments arranged by size: 2008 1791 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 3602 1870 Size: 73 1659 4545 Fragments arranged by size: 4545 1659 73 AvrII C'CTAG_G Cuts at: 3556 3556 Size: 6277 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3598 5053 618 Size: 325 217 627 1811 1455 1842 Fragments arranged by size: 1842 1811 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2422 3596 1391 Size: 1031 1174 4072 Fragments arranged by size: 4072 1174 1031 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5306 BbvCI CC'TCA_GC Cuts at: 2250 2250 Size: 6277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2317 4169 5379 2317 Size: 1852 1210 3215 Fragments arranged by size: 3215 1852 1210 BclI T'GATC_A Cuts at: 1548 2094 1548 Size: 546 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4888 136 Size: 107 122 71 631 605 3216 1525 Fragments arranged by size: 3216 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 2943 3517 3694 2943 Size: 574 177 5526 Fragments arranged by size: 5526 574 177 Bme1580I G_kGCm'C Cuts at: 1790 4338 5494 1790 Size: 2548 1156 2573 Fragments arranged by size: 2573 2548 1156 BmgBI CAC'GTC Cuts at: 1318 3299 1318 Size: 1981 4296 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2034 4008 4966 Size: 416 53 83 399 24 617 1974 958 Cuts at: 4966 442 Size: 1753 Fragments arranged by size: 1974 1753 958 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3435 4937 1239 Size: 2196 1502 2579 Fragments arranged by size: 2579 2196 1502 Bpu10I CC'TnA_GC Cuts at: 2250 2250 Size: 6277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4402 5267 5565 4402 Size: 865 298 5114 Fragments arranged by size: 5114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4940 882 Size: 617 3441 2219 Fragments arranged by size: 3441 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4068 493 Size: 711 2864 2702 Fragments arranged by size: 2864 2702 711 BsaBI GATnn'nnATC Cuts at: 3212 6271 3212 Size: 3059 3218 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4084 4466 5101 275 Size: 53 83 186 936 2551 382 635 1451 Fragments arranged by size: 2551 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2954 4710 5108 5382 5529 5940 2954 Size: 1756 398 274 147 411 3291 Fragments arranged by size: 3291 1756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6052 Fragments arranged by size: 6052 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6277 BseYI C'CCAG_C Cuts at: 2059 5480 5976 2059 Size: 3421 496 2360 Fragments arranged by size: 3421 2360 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5704 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4488 5516 1540 Size: 275 186 1726 761 1028 2301 Fragments arranged by size: 2301 1726 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2422 2566 3423 4338 4423 5494 2422 Size: 144 857 915 85 1071 3205 Fragments arranged by size: 3205 1071 915 857 144 85 BsmI GAATG_Cn' Cuts at: 2601 3323 3788 3881 2601 Size: 722 465 93 4997 Fragments arranged by size: 4997 722 465 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2422 2566 3423 3596 4338 4423 5494 Size: 399 632 144 857 173 742 85 1071 Cuts at: 5494 1391 Size: 2174 Fragments arranged by size: 2174 1071 857 742 632 399 173 144 85 BspEI T'CCGG_A Cuts at: 2954 5940 2954 Size: 2986 3291 BspHI T'CATG_A Cuts at: 4166 5896 4166 Size: 1730 4547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3347 3632 844 Size: 2503 285 3489 Fragments arranged by size: 3489 2503 285 BsrBI CCG'CTC Cuts at: 1394 1823 2552 4164 1394 Size: 429 729 1612 3507 Fragments arranged by size: 3507 1612 729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3372 4772 4946 65 Size: 1183 2124 1400 174 1396 Fragments arranged by size: 2124 1400 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4921 5108 1700 Size: 110 3111 187 2869 Fragments arranged by size: 3111 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 4337 5349 4337 Size: 1012 5265 BstAPI GCAn_nnn'nTGC Cuts at: 794 2665 3320 794 Size: 1871 655 3751 Fragments arranged by size: 3751 1871 655 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6277 BstXI CCAn_nnnn'nTGG Cuts at: 2244 2244 Size: 6277 BstYI r'GATC_y Cuts at: 1972 3607 3974 4359 4376 5828 5914 5926 6272 Size: 1635 367 385 17 1452 86 12 346 Cuts at: 6272 1972 Size: 1977 Fragments arranged by size: 1977 1635 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5725 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 2049 3060 3793 4587 4607 2049 Size: 1011 733 794 20 3719 Fragments arranged by size: 3719 1011 794 733 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 3451 3581 3451 Size: 130 6147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5284 809 Size: 507 3968 1802 Fragments arranged by size: 3968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2586 3724 4613 Size: 54 1425 50 275 18 756 1138 889 Cuts at: 4613 6242 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1138 889 756 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 2891 3503 4207 1353 Size: 897 641 612 704 3423 Fragments arranged by size: 3423 897 704 641 612 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4864 5238 5384 387 Size: 1064 138 3275 374 146 1280 Fragments arranged by size: 3275 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2420 2420 Size: 6277 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2681 3435 4340 4937 5724 5992 1239 Size: 393 1049 754 905 597 787 268 1524 Fragments arranged by size: 1524 1049 905 787 754 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2028 2028 Size: 6277 EcoRI G'AATT_C Cuts at: 3497 3586 3964 3497 Size: 89 378 5810 Fragments arranged by size: 5810 378 89 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 3045 5424 1678 Size: 1367 2379 2531 Fragments arranged by size: 2531 2379 1367 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2608 2640 3090 3122 4918 Size: 32 135 32 1036 32 450 32 1796 Cuts at: 4918 4950 4992 5024 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1796 1036 450 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3621 3867 669 Size: 988 281 1683 246 3079 Fragments arranged by size: 3079 1683 988 281 246 HindIII A'AGCT_T Cuts at: 748 2779 748 Size: 2031 4246 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3331 3876 3331 Size: 545 5732 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2395 2570 5391 5575 1305 Size: 1090 175 2821 184 2007 Fragments arranged by size: 2821 2007 1090 184 175 MscI TGG'CCA Cuts at: 10 64 1489 1832 2588 10 Size: 54 1425 343 756 3699 Fragments arranged by size: 3699 1425 756 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 2090 3149 3155 4235 4594 4753 Size: 648 888 36 1059 6 1080 359 159 Cuts at: 4753 518 Size: 2042 Fragments arranged by size: 2042 1080 1059 888 648 359 159 36 6 MspA1I CmG'CkG Cuts at: 1606 2270 2584 3693 4370 5518 5763 1606 Size: 664 314 1109 677 1148 245 2120 Fragments arranged by size: 2120 1148 1109 677 664 314 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5725 NdeI CA'TA_TG Cuts at: 387 387 Size: 6277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 2138 2939 3635 5180 1132 Size: 97 909 801 696 1545 2229 Fragments arranged by size: 2229 1545 909 801 696 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6277 PciI A'CATG_T Cuts at: 2134 5176 2134 Size: 3042 3235 PflMI CCAn_nnn'nTGG Cuts at: 1108 2292 1108 Size: 1184 5093 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2028 2028 Size: 6277 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6277 PsiI TTA'TAA Cuts at: 2465 3847 2465 Size: 1382 4895 PstI C_TGCA'G Cuts at: 830 3496 3629 830 Size: 2666 133 3478 Fragments arranged by size: 3478 2666 133 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2270 2584 2270 Size: 314 5963 SacI G_AGCT'C Cuts at: 2422 2422 Size: 6277 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SanDI GG'GwC_CC Cuts at: 2028 2028 Size: 6277 SapI GCTCTTCn'nnn_ Cuts at: 2891 2891 Size: 6277 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SfcI C'TryA_G Cuts at: 826 1045 2128 3492 3540 3564 3625 4760 5441 Size: 219 1083 1364 48 24 61 1135 681 Cuts at: 5441 5632 826 Size: 191 1471 Fragments arranged by size: 1471 1364 1135 1083 681 219 191 61 48 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 3604 1872 Size: 1732 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4381 5282 5544 820 Size: 197 926 2438 901 262 1553 Fragments arranged by size: 2438 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 4201 5 Size: 47 4149 2081 Fragments arranged by size: 4149 2081 47 StuI AGG'CCT Cuts at: 1517 2514 1517 Size: 997 5280 StyI C'CwwG_G Cuts at: 513 1065 2243 3556 3716 513 Size: 552 1178 1313 160 3074 Fragments arranged by size: 3074 1313 1178 552 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2132 2175 2340 4304 4321 4474 4659 Size: 1255 552 43 165 1964 17 153 185 Cuts at: 4659 5104 325 Size: 445 1498 Fragments arranged by size: 1964 1498 1255 552 445 185 165 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2192 3369 4523 Size: 274 80 33 51 1443 215 1177 1154 Cuts at: 4523 6235 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1177 1154 274 215 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3381 3413 6237 6269 3381 Size: 32 2824 32 3389 Fragments arranged by size: 3389 2824 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3297 1316 Size: 1981 4296 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3509 3509 Size: 6277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 3602 1870 Size: 1732 4545 XmnI GAAnn'nnTTC Cuts at: 3179 4406 3179 Size: 1227 5050 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI