(Circular) (Six-base) MAPSORT of: pFN21AB7854.seq Check: 6419 from: 1 to: 6580 pFN21AB7854 6580 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4390 278 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3923 1937 Size: 1986 4594 Acc65I G'GTAC_C Cuts at: 3901 3901 Size: 6580 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2505 2682 2899 4643 6027 6295 1632 Size: 873 177 217 1744 1384 268 1917 Fragments arranged by size: 1917 1744 1384 873 268 217 177 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6383 AflIII A'CryG_T Cuts at: 1203 2718 5479 6456 1203 Size: 1515 2761 977 1327 Fragments arranged by size: 2761 1515 1327 977 AgeI A'CCGG_T Cuts at: 5411 5411 Size: 6580 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 2474 1166 Size: 1308 5272 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6580 AlwNI CAG_nnn'CTG Cuts at: 4323 5895 4323 Size: 1572 5008 ApaI G_GGCC'C Cuts at: 2801 2801 Size: 6580 ApaLI G'TGCA_C Cuts at: 2519 4637 5793 2519 Size: 2118 1156 3306 Fragments arranged by size: 3306 2118 1156 ApoI r'AATT_y Cuts at: 1493 2401 2684 3889 4115 4267 6376 1493 Size: 908 283 1205 226 152 2109 1697 Fragments arranged by size: 2109 1697 1205 908 283 226 152 AseI AT'TA_AT Cuts at: 160 1032 2166 5134 160 Size: 872 1134 2968 1606 Fragments arranged by size: 2968 1606 1134 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 2226 2435 2529 3905 1870 Size: 73 283 209 94 1376 4545 Fragments arranged by size: 4545 1376 283 209 94 73 BamHI G'GATC_C Cuts at: 3910 4277 3910 Size: 367 6213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2300 3901 5356 618 Size: 325 217 627 513 1601 1455 1842 Fragments arranged by size: 1842 1601 1455 627 513 325 217 BanII G_rGCy'C Cuts at: 1391 2444 2801 3899 1391 Size: 1053 357 1098 4072 Fragments arranged by size: 4072 1098 1053 357 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2280 2905 2928 3241 928 Size: 971 381 625 23 313 4267 Fragments arranged by size: 4267 971 625 381 313 23 BbvCI CC'TCA_GC Cuts at: 2709 2709 Size: 6580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2426 2460 4771 4805 1903 Size: 34 489 34 2311 34 3678 Fragments arranged by size: 3678 2311 489 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4472 5682 4472 Size: 1210 5370 BclI T'GATC_A Cuts at: 1548 2725 1548 Size: 1177 5403 BfrBI ATG'CAT Cuts at: 3659 3659 Size: 6580 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2150 5191 136 Size: 107 122 71 631 605 478 3041 1525 Fragments arranged by size: 3041 1525 631 605 478 122 107 71 BglII A'GATC_T Cuts at: 6575 6575 Size: 6580 BlpI GC'TnA_GC Cuts at: 3997 3997 Size: 6580 Bme1580I G_kGCm'C Cuts at: 1790 2052 2523 2589 2801 4641 5797 1790 Size: 262 471 66 212 1840 1156 2573 Fragments arranged by size: 2573 1840 1156 471 262 212 66 BmgBI CAC'GTC Cuts at: 1318 2584 3085 1318 Size: 1266 501 4813 Fragments arranged by size: 4813 1266 501 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2052 4311 5269 Size: 416 53 83 399 24 635 2259 958 Cuts at: 5269 442 Size: 1753 Fragments arranged by size: 2259 1753 958 635 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3823 3823 Size: 6580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 5240 1239 Size: 1178 2823 2579 Fragments arranged by size: 2823 2579 1178 Bpu10I CC'TnA_GC Cuts at: 2709 2709 Size: 6580 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3145 4705 5570 5868 3145 Size: 1560 865 298 3857 Fragments arranged by size: 3857 1560 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2843 5243 882 Size: 617 1344 2400 2219 Fragments arranged by size: 2400 2219 1344 617 BsaAI yAC'GTr Cuts at: 493 1204 3628 4371 493 Size: 711 2424 743 2702 Fragments arranged by size: 2702 2424 743 711 BsaBI GATnn'nnATC Cuts at: 3107 6574 3107 Size: 3467 3113 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4387 4769 5404 275 Size: 53 83 186 936 2854 382 635 1451 Fragments arranged by size: 2854 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2638 2852 3482 5013 5411 5685 5832 6243 2638 Size: 214 630 1531 398 274 147 411 2975 Fragments arranged by size: 2975 1531 630 411 398 274 214 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2705 2735 1178 Size: 30 165 30 1302 30 5023 Fragments arranged by size: 5023 1302 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2346 2726 3130 3340 3578 1186 Size: 1160 380 404 210 238 4188 Fragments arranged by size: 4188 1160 404 380 238 210 BseYI C'CCAG_C Cuts at: 5783 6279 5783 Size: 496 6084 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6007 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4030 4791 5819 1540 Size: 275 186 2029 761 1028 2301 Fragments arranged by size: 2301 2029 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2444 2523 3131 3621 3804 4641 4726 5797 2444 Size: 79 608 490 183 837 85 1071 3227 Fragments arranged by size: 3227 1071 837 608 490 183 85 79 BsmI GAATG_Cn' Cuts at: 4091 4184 4091 Size: 93 6487 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2444 2523 2589 2801 3131 3621 Size: 399 262 392 79 66 212 330 490 Cuts at: 3621 3804 3899 4641 4726 5797 1391 Size: 183 95 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 490 399 392 330 262 212 183 95 85 79 66 BspEI T'CCGG_A Cuts at: 2638 6243 2638 Size: 3605 2975 BspHI T'CATG_A Cuts at: 4469 6199 4469 Size: 1730 4850 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3480 3935 844 Size: 2636 455 3489 Fragments arranged by size: 3489 2636 455 BsrBI CCG'CTC Cuts at: 1394 1823 3292 4467 1394 Size: 429 1469 1175 3507 Fragments arranged by size: 3507 1469 1175 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3003 5075 5249 65 Size: 1183 1755 2072 174 1396 Fragments arranged by size: 2072 1755 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5224 5411 1700 Size: 110 3414 187 2869 Fragments arranged by size: 3414 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6580 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6580 BssSI C'ACGA_G Cuts at: 4640 5652 4640 Size: 1012 5568 BstAPI GCAn_nnn'nTGC Cuts at: 794 3062 794 Size: 2268 4312 BstBI TT'CG_AA Cuts at: 5384 5384 Size: 6580 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6580 BstYI r'GATC_y Cuts at: 1972 3910 4277 4662 4679 6131 6217 6229 6575 Size: 1938 367 385 17 1452 86 12 346 Cuts at: 6575 1972 Size: 1977 Fragments arranged by size: 1977 1938 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3555 3726 513 Size: 552 2490 171 3367 Fragments arranged by size: 3367 2490 552 171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5841 Fragments arranged by size: 5841 683 56 BtsI GCAGTG_nn' Cuts at: 3532 4096 4890 4910 3532 Size: 564 794 20 5202 Fragments arranged by size: 5202 794 564 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6545 DraI TTT'AAA Cuts at: 3884 3884 Size: 6580 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5587 809 Size: 507 4271 1802 Fragments arranged by size: 4271 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2595 4027 4916 Size: 54 1425 50 275 18 765 1432 889 Cuts at: 4916 6545 8 Size: 1629 43 Fragments arranged by size: 1629 1432 1425 889 765 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4027 1537 Size: 275 2215 4090 Fragments arranged by size: 4090 2215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2077 2538 3042 3087 3282 3383 3449 3555 Size: 724 461 504 45 195 101 66 106 Cuts at: 3555 4510 1353 Size: 955 3423 Fragments arranged by size: 3423 955 724 504 461 195 106 101 66 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5167 5541 5687 387 Size: 1064 138 3578 374 146 1280 Fragments arranged by size: 3578 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2442 2442 Size: 6580 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2505 2682 2899 4643 5240 6027 Size: 393 785 88 177 217 1744 597 787 Cuts at: 6027 6295 1239 Size: 268 1524 Fragments arranged by size: 1744 1524 787 785 597 393 268 217 177 88 EcoO109I rG'GnC_Cy Cuts at: 2797 2798 2995 3688 2797 Size: 1 197 693 5689 Fragments arranged by size: 5689 693 197 1 EcoRI G'AATT_C Cuts at: 2401 3889 4267 2401 Size: 1488 378 4714 Fragments arranged by size: 4714 1488 378 EcoRV GAT'ATC Cuts at: 3705 3705 Size: 6580 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2137 3040 2137 Size: 903 5677 FspI TGC'GCA Cuts at: 5086 5086 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 2610 5727 1678 Size: 932 3117 2531 Fragments arranged by size: 3117 2531 932 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2334 2366 2544 2573 2576 Size: 32 135 32 762 32 178 29 3 Cuts at: 2576 2605 2717 2749 2865 2897 3125 3157 3413 Size: 29 112 32 116 32 228 32 256 Cuts at: 3413 3445 5221 5253 5295 5327 1373 Size: 32 1776 32 42 32 2626 Fragments arranged by size: 2626 1776 762 256 228 178 135 116 112 42 32 32 32 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3924 4170 669 Size: 988 281 1986 246 3079 Fragments arranged by size: 3079 1986 988 281 246 HindIII A'AGCT_T Cuts at: 748 3743 748 Size: 2995 3585 HpaI GTT'AAC Cuts at: 4170 4170 Size: 6580 KpnI G_GTAC'C Cuts at: 3905 3905 Size: 6580 MfeI C'AATT_G Cuts at: 4179 4179 Size: 6580 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1998 2896 5694 5878 1305 Size: 693 898 2798 184 2007 Fragments arranged by size: 2798 2007 898 693 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2597 10 Size: 54 1425 343 765 3993 Fragments arranged by size: 3993 1425 765 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2456 2474 3113 3773 3869 4538 4897 Size: 648 1290 18 639 660 96 669 359 Cuts at: 4897 5056 518 Size: 159 2042 Fragments arranged by size: 2042 1290 669 660 648 639 359 159 96 18 MspA1I CmG'CkG Cuts at: 1606 3996 4673 5821 6066 1606 Size: 2390 677 1148 245 2120 Fragments arranged by size: 2390 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3555 513 Size: 552 2490 3538 Fragments arranged by size: 3538 2490 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6580 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NsiI A_TGCA'T Cuts at: 3661 3661 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 2722 3938 5483 1132 Size: 97 1493 1216 1545 2229 Fragments arranged by size: 2229 1545 1493 1216 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6580 PciI A'CATG_T Cuts at: 2718 5479 2718 Size: 2761 3819 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6580 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4649 4681 6162 6194 4649 Size: 32 1481 32 5035 Fragments arranged by size: 5035 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2995 2995 Size: 6580 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6580 PsiI TTA'TAA Cuts at: 3198 4150 3198 Size: 952 5628 PspOMI G'GGCC_C Cuts at: 2797 2797 Size: 6580 PstI C_TGCA'G Cuts at: 830 3477 3932 830 Size: 2647 455 3478 Fragments arranged by size: 3478 2647 455 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6580 SacI G_AGCT'C Cuts at: 2444 2444 Size: 6580 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SapI GCTCTTCn'nnn_ Cuts at: 2077 2538 3282 2077 Size: 461 744 5375 Fragments arranged by size: 5375 744 461 SbfI CC_TGCA'GG Cuts at: 3932 3932 Size: 6580 ScaI AGT'ACT Cuts at: 4828 6540 4828 Size: 1712 4868 SfcI C'TryA_G Cuts at: 826 1045 3473 3928 5063 5744 5935 826 Size: 219 2428 455 1135 681 191 1471 Fragments arranged by size: 2428 1471 1135 681 455 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 2531 3907 1872 Size: 659 1376 4545 Fragments arranged by size: 4545 1376 659 SmlI C'TyrA_G Cuts at: 820 1017 1943 2226 3124 4684 5585 5847 820 Size: 197 926 283 898 1560 901 262 1553 Fragments arranged by size: 1560 1553 926 901 898 283 262 197 SnaBI TAC'GTA Cuts at: 493 4371 493 Size: 3878 2702 SpeI A'CTAG_T Cuts at: 152 2035 152 Size: 1883 4697 SphI G_CATG'C Cuts at: 1132 3938 1132 Size: 2806 3774 SspI AAT'ATT Cuts at: 5 52 4504 5 Size: 47 4452 2081 Fragments arranged by size: 4452 2081 47 StuI AGG'CCT Cuts at: 1517 3326 1517 Size: 1809 4771 StyI C'CwwG_G Cuts at: 513 1065 2148 2296 3555 4019 513 Size: 552 1083 148 1259 464 3074 Fragments arranged by size: 3074 1259 1083 552 464 148 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 4607 4624 4777 4962 5407 325 Size: 1255 1035 1992 17 153 185 445 1498 Fragments arranged by size: 1992 1498 1255 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 2715 2751 Size: 274 80 33 51 1443 441 297 36 Cuts at: 2751 4826 6538 96 Size: 2075 1712 138 Fragments arranged by size: 2075 1712 1443 441 297 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2457 2489 6540 6572 2457 Size: 32 4051 32 2465 Fragments arranged by size: 4051 2465 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6580 XbaI T'CTAG_A Cuts at: 3916 3916 Size: 6580 XhoI C'TCGA_G Cuts at: 1943 2226 1943 Size: 283 6297 XmaI C'CCGG_G Cuts at: 1870 2529 3905 1870 Size: 659 1376 4545 Fragments arranged by size: 4545 1376 659 XmnI GAAnn'nnTTC Cuts at: 3104 4709 3104 Size: 1605 4975 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4388 276 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI