Restriction map for
pFN21AE0755
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI BalI BbrPI BcgI BciVI
BclI BfrI BglII BsaAI BsaBI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
HincII HindII HindIII HinfI HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AgeI 274 ACCGGT A^CCGGT
AhdI 226 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 389 CCTCAGG CC^T[ACGT]AGG
AspEI 226 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 623 TGCGCA TGC^GCA
AvrII 886 CCTAGG C^CTAGG
BamHI 394 GGATCC G^GATCC
BbeI 878 GGCGCC GGCGC^C
BbsI 840 GAAGAC GAAGAC(2/6)
BfaI 887 CTAG C^TAG
BlnI 886 CCTAGG C^CTAGG
BlpI 322 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 322 GCTCAGC GC^T[ACGT]AGC
BpuAI 840 GAAGAC GAAGAC(2/6)
BsaI 13 GAGACC GGTCTC(1/5)
BsaWI 274 ACCGGT [AT]^CCGG[AT]
BstAPI 62 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 394 GGATCC [GA]^GATC[CT]
Bsu36I 389 CCTCAGG CC^T[ACGT]AGG
CelII 322 GCTCAGC GC^T[ACGT]AGC
DpnII 395 GATC ^GATC
EaeI 189 TGGCCG [CT]^GGCC[GA]
Eam1105I 226 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 389 CCTCAGG CC^T[ACGT]AGG
FseI 533 GGCCGGCC GGCCGG^CC
FspI 623 TGCGCA TGC^GCA
KasI 878 GGCGCC G^GCGCC
MaeI 887 CTAG C^TAG
MaeII 704 ACGT A^CGT
MaeIII 170 GTGAC ^GT[ACGT]AC
MboI 395 GATC ^GATC
MboII 840 GAAGA GAAGA(8/7)
MflI 394 GGATCC [GA]^GATC[CT]
MslI 769 CACAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 878 GGCGCC GG^CGCC
NcoI 434 CCATGG C^CATGG
NdeII 395 GATC ^GATC
NspI 48 GCATGT [GA]CATG^[CT]
PinAI 274 ACCGGT A^CCGGT
Sau3AI 395 GATC ^GATC
SfcI 600 CTACAG C^T[GA][CT]AG
SfoI 878 GGCGCC GGC^GCC
SgrAI 273 CACCGGTG C[GA]^CCGG[CT]G
Tsp45I 170 GTGAC ^GT[GC]AC
TspRI 749 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 394 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 395 GATCC GGATC(4/5)
394 GGATC
AlwNI 182 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
755 CAGGCGCTG
ApaI 345 GGGCCC GGGCC^C
552 GGGCCC
AvaI 266 CCCGGG C^[CT]CG[GA]G
369 CCCGGG
BbvCI 959 CCTCAGC CCTCAGC(-5/-2)
570 GCTGAGG
BsgI 965 CTGCAC GTGCAG(16/14)
493 GTGCAG
BsoBI 266 CCCGGG C^[CT]CG[GA]G
369 CCCGGG
BsrI 315 ACTGG ACTGG(1/-1)
408 CCAGT
DsaI 434 CCATGG C^C[GA][CT]GG
485 CCGTGG
FauI 515 CCCGC CCCGC(4/6)
166 GCGGG
HaeII 757 GGCGCT [GA]GCGC^[CT]
878 GGCGCC
PflMI 309 CCATCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
754 CCAGGCGCTGG
SfaNI 403 GATGC GCATC(5/9)
32 GCATC
SmaI 266 CCCGGG CCC^GGG
369 CCCGGG
StuI 335 AGGCCT AGG^CCT
383 AGGCCT
Van91I 309 CCATCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
754 CCAGGCGCTGG
XcmI 350 CCATCTGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
416 CCAAGAGGGGCCTGG
XmaCI 266 CCCGGG C^CCGGG
369 CCCGGG
XmaI 266 CCCGGG C^CCGGG
369 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcyI AluI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MnlI MspA1I
MspI MvaI MwoI NaeI NciI
NgoMIV NlaIII NlaIV PvuII Sau96I
ScrFI StyI TseI