(Circular) (Six-base) MAPSORT of: pFN21AE0755.seq Check: 6619 from: 1 to: 5692 pFN21AE0755 5692 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3035 1937 Size: 1098 4594 Acc65I G'GTAC_C Cuts at: 3013 3013 Size: 5692 AclI AA'CG_TT Cuts at: 1563 3819 4192 1563 Size: 2256 373 3063 Fragments arranged by size: 3063 2256 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2552 2864 3755 5139 5407 1632 Size: 920 312 891 1384 268 1917 Fragments arranged by size: 1917 1384 920 891 312 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 4591 5568 1203 Size: 3388 977 1327 Fragments arranged by size: 3388 1327 977 AgeI A'CCGG_T Cuts at: 2279 4523 2279 Size: 2244 3448 AhdI GACnn_n'nnGTC Cuts at: 2236 4421 2236 Size: 2185 3507 AleI CACnn'nnGTG Cuts at: 1166 2778 1166 Size: 1612 4080 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5692 AlwNI CAG_nnn'CTG Cuts at: 2192 2765 3435 5007 2192 Size: 573 670 1572 2877 Fragments arranged by size: 2877 1572 670 573 ApaI G_GGCC'C Cuts at: 2354 2561 2354 Size: 207 5485 ApaLI G'TGCA_C Cuts at: 3749 4905 3749 Size: 1156 4536 ApoI r'AATT_y Cuts at: 1493 3001 3227 3379 5488 1493 Size: 1508 226 152 2109 1697 Fragments arranged by size: 2109 1697 1508 226 152 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 2271 2374 3017 1870 Size: 73 328 103 643 4545 Fragments arranged by size: 4545 643 328 103 73 AvrII C'CTAG_G Cuts at: 2891 2891 Size: 5692 BamHI G'GATC_C Cuts at: 2399 3022 3389 2399 Size: 623 367 4702 Fragments arranged by size: 4702 623 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 2591 2883 3013 4468 Size: 325 217 627 495 309 292 130 1455 Cuts at: 4468 618 Size: 1842 Fragments arranged by size: 1842 1455 627 495 325 309 292 217 130 BanII G_rGCy'C Cuts at: 1391 2095 2354 2561 2933 3011 1391 Size: 704 259 207 372 78 4072 Fragments arranged by size: 4072 704 372 259 207 78 BbeI G_GCGC'C Cuts at: 2887 2887 Size: 5692 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2852 928 Size: 971 953 3768 Fragments arranged by size: 3768 971 953 BbvCI CC'TCA_GC Cuts at: 2576 2965 2576 Size: 389 5303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4794 3584 Size: 1210 4482 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2118 2197 2928 Size: 107 122 71 631 605 446 79 731 Cuts at: 2928 4303 136 Size: 1375 1525 Fragments arranged by size: 1525 1375 731 631 605 446 122 107 79 71 BglII A'GATC_T Cuts at: 5687 5687 Size: 5692 BlpI GC'TnA_GC Cuts at: 2328 3109 2328 Size: 781 4911 Bme1580I G_kGCm'C Cuts at: 1790 2354 2561 2594 3753 4909 1790 Size: 564 207 33 1159 1156 2573 Fragments arranged by size: 2573 1159 1156 564 207 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2407 3423 4381 Size: 416 53 83 399 24 990 1016 958 Cuts at: 4381 442 Size: 1753 Fragments arranged by size: 1753 1016 990 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2410 2467 2410 Size: 57 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2153 2909 4352 1239 Size: 797 117 756 1443 2579 Fragments arranged by size: 2579 1443 797 756 117 Bpu10I CC'TnA_GC Cuts at: 2576 2965 2576 Size: 389 5303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3817 4682 4980 3817 Size: 865 298 4529 Fragments arranged by size: 4529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2012 4355 882 Size: 617 513 2343 2219 Fragments arranged by size: 2343 2219 617 513 BsaAI yAC'GTr Cuts at: 493 1204 3483 493 Size: 711 2279 2702 Fragments arranged by size: 2702 2279 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2565 2670 2884 3499 Size: 53 83 186 936 1032 105 214 615 Cuts at: 3499 3881 4516 275 Size: 382 635 1451 Fragments arranged by size: 1451 1032 936 635 615 382 214 186 105 83 53 BsaWI w'CCGG_w Cuts at: 2279 4125 4523 4797 4944 5355 2279 Size: 1846 398 274 147 411 2616 Fragments arranged by size: 2616 1846 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2942 2972 1178 Size: 30 165 30 1539 30 3898 Fragments arranged by size: 3898 1539 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2383 2452 2669 1186 Size: 1197 69 217 4209 Fragments arranged by size: 4209 1197 217 69 BseYI C'CCAG_C Cuts at: 2139 2554 2765 2941 4895 5391 2139 Size: 415 211 176 1954 496 2440 Fragments arranged by size: 2440 1954 496 415 211 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2519 2955 1163 Size: 573 783 436 3900 Fragments arranged by size: 3900 783 573 436 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3142 3903 4931 1540 Size: 275 186 1141 761 1028 2301 Fragments arranged by size: 2301 1141 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3753 3838 4909 3753 Size: 85 1071 4536 Fragments arranged by size: 4536 1071 85 BsmI GAATG_Cn' Cuts at: 3203 3296 3203 Size: 93 5599 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 2354 2561 2594 2933 3011 3753 Size: 399 305 259 207 33 339 78 742 Cuts at: 3753 3838 4909 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 339 305 259 207 85 78 33 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3047 844 Size: 2203 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3579 1394 Size: 429 1756 3507 Fragments arranged by size: 3507 1756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4187 4361 65 Size: 1183 2939 174 1396 Fragments arranged by size: 2939 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2196 2248 2279 2539 4336 4523 1700 Size: 110 386 52 31 260 1797 187 2869 Fragments arranged by size: 2869 1797 386 260 187 110 52 31 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5692 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 2073 794 Size: 1279 4413 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5692 BstYI r'GATC_y Cuts at: 1972 2399 3022 3389 3774 3791 5243 5329 5341 Size: 427 623 367 385 17 1452 86 12 Cuts at: 5341 5687 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 623 427 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2395 2395 Size: 5692 BtgI C'CryG_G Cuts at: 513 1065 2004 2439 2490 513 Size: 552 939 435 51 3715 Fragments arranged by size: 3715 939 552 435 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4953 Fragments arranged by size: 4953 683 56 BtsI GCAGTG_nn' Cuts at: 3208 4002 4022 3208 Size: 794 20 4878 Fragments arranged by size: 4878 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2194 3139 4028 Size: 54 1425 50 275 18 364 945 889 Cuts at: 4028 5657 8 Size: 1629 43 Fragments arranged by size: 1629 1425 945 889 364 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3139 1537 Size: 275 1327 4090 Fragments arranged by size: 4090 1327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3622 1353 Size: 2269 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2095 2210 4279 4653 4799 387 Size: 1064 138 506 115 2069 374 146 1280 Fragments arranged by size: 2069 1280 1064 506 374 146 138 115 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2153 2552 2864 2909 3755 4352 Size: 393 404 117 399 312 45 846 597 Cuts at: 4352 5139 5407 1239 Size: 787 268 1524 Fragments arranged by size: 1524 846 787 597 404 399 393 312 268 117 45 EcoO109I rG'GnC_Cy Cuts at: 2189 2351 2428 2599 2189 Size: 162 77 171 5282 Fragments arranged by size: 5282 171 162 77 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FseI GG_CCGG'CC Cuts at: 2543 2543 Size: 5692 FspI TGC'GCA Cuts at: 2630 4198 2630 Size: 1568 4124 HaeII r_GCGC'y Cuts at: 1678 2766 2887 4839 1678 Size: 1088 121 1952 2531 Fragments arranged by size: 2531 1952 1088 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2202 2222 2234 2254 2399 Size: 32 135 32 630 20 12 20 145 Cuts at: 2399 2431 2696 2728 4333 4365 4407 4439 1373 Size: 32 265 32 1605 32 42 32 2626 Fragments arranged by size: 2626 1605 630 265 145 135 42 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3036 3282 669 Size: 988 281 1098 246 3079 Fragments arranged by size: 3079 1098 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KasI G'GCGC_C Cuts at: 2883 2883 Size: 5692 KpnI G_GTAC'C Cuts at: 3017 3017 Size: 5692 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2667 4806 4990 1305 Size: 1362 2139 184 2007 Fragments arranged by size: 2139 2007 1362 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3870 Fragments arranged by size: 3870 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2778 3650 4009 4168 518 Size: 648 1612 872 359 159 2042 Fragments arranged by size: 2042 1612 872 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2171 2365 2523 2530 2575 2610 2969 3108 Size: 565 194 158 7 45 35 359 139 Cuts at: 3108 3785 4933 5178 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 565 359 245 194 158 139 45 35 7 NaeI GCC'GGC Cuts at: 2198 2250 2541 2198 Size: 52 291 5349 Fragments arranged by size: 5349 291 52 NarI GG'CG_CC Cuts at: 2884 2884 Size: 5692 NcoI C'CATG_G Cuts at: 513 1065 2004 2439 513 Size: 552 939 435 3766 Fragments arranged by size: 3766 939 552 435 NdeI CA'TA_TG Cuts at: 387 387 Size: 5692 NgoMIV G'CCGG_C Cuts at: 2196 2248 2539 2196 Size: 52 291 5349 Fragments arranged by size: 5349 291 52 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 3139 3139 Size: 5692 NspI r_CATG'y Cuts at: 1132 1229 2057 3050 4595 1132 Size: 97 828 993 1545 2229 Fragments arranged by size: 2229 1545 993 828 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5692 PciI A'CATG_T Cuts at: 4591 4591 Size: 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 2765 1108 Size: 1212 445 4035 Fragments arranged by size: 4035 1212 445 PfoI T'CCnGG_A Cuts at: 2148 2808 2148 Size: 660 5032 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PspOMI G'GGCC_C Cuts at: 2350 2557 2350 Size: 207 5485 PstI C_TGCA'G Cuts at: 830 3044 830 Size: 2214 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 PvuII CAG'CTG Cuts at: 2365 2530 2575 2610 2969 2365 Size: 165 45 35 359 5088 Fragments arranged by size: 5088 359 165 45 35 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SfcI C'TryA_G Cuts at: 826 1045 2605 3040 4175 4856 5047 826 Size: 219 1560 435 1135 681 191 1471 Fragments arranged by size: 1560 1471 1135 681 435 219 191 SfoI GGC'GCC Cuts at: 2885 2885 Size: 5692 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 2279 1700 Size: 579 5113 SmaI CCC'GGG Cuts at: 1872 2273 2376 3019 1872 Size: 401 103 643 4545 Fragments arranged by size: 4545 643 401 103 SmlI C'TyrA_G Cuts at: 820 1017 1943 3796 4697 4959 820 Size: 197 926 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 2342 2390 1517 Size: 825 48 4819 Fragments arranged by size: 4819 825 48 StyI C'CwwG_G Cuts at: 513 1065 2004 2439 2586 2794 2891 2980 3131 Size: 552 939 435 147 208 97 89 151 Cuts at: 3131 513 Size: 3074 Fragments arranged by size: 3074 939 552 435 208 151 147 97 89 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2609 3719 3736 3889 4074 4519 325 Size: 1255 1029 1110 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1110 1029 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3938 5650 96 Size: 274 80 33 51 1443 1961 1712 138 Fragments arranged by size: 1961 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2942 2974 5652 5684 2942 Size: 32 2678 32 2950 Fragments arranged by size: 2950 2678 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5692 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2362 2428 2011 Size: 351 66 5275 Fragments arranged by size: 5275 351 66 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 2271 2374 3017 1870 Size: 401 103 643 4545 Fragments arranged by size: 4545 643 401 103 XmnI GAAnn'nnTTC Cuts at: 3821 3821 Size: 5692 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI