Restriction map for
pFN21AE0755
(Linear) (Six-base) MAPSORT of: KIEE0755.seq Check: 6106 from: 1 to: 987
KIEE0755.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 859 987
Size: 547 312 128
Fragments arranged by size:
547 312 128
AgeI A'CCGG_T
Cuts at: 0 274 987
Size: 274 713
AhdI GACnn_n'nnGTC
Cuts at: 0 231 987
Size: 231 756
AleI CACnn'nnGTG
Cuts at: 0 773 987
Size: 773 214
AlwNI CAG_nnn'CTG
Cuts at: 0 187 760 987
Size: 187 573 227
Fragments arranged by size:
573 227 187
ApaI G_GGCC'C
Cuts at: 0 349 556 987
Size: 349 207 431
Fragments arranged by size:
431 349 207
AvaI C'yCGr_G
Cuts at: 0 266 369 987
Size: 266 103 618
Fragments arranged by size:
618 266 103
AvrII C'CTAG_G
Cuts at: 0 886 987
Size: 886 101
BamHI G'GATC_C
Cuts at: 0 394 987
Size: 394 593
BanI G'GyrC_C
Cuts at: 0 277 586 878 987
Size: 277 309 292 109
Fragments arranged by size:
309 292 277 109
BanII G_rGCy'C
Cuts at: 0 90 349 556 928 987
Size: 90 259 207 372 59
Fragments arranged by size:
372 259 207 90 59
BbeI G_GCGC'C
Cuts at: 0 882 987
Size: 882 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 847 987
Size: 847 140
BbvCI CC'TCA_GC
Cuts at: 0 571 960 987
Size: 571 389 27
Fragments arranged by size:
571 389 27
BglI GCCn_nnn'nGGC
Cuts at: 0 113 192 923 987
Size: 113 79 731 64
Fragments arranged by size:
731 113 79 64
BlpI GC'TnA_GC
Cuts at: 0 323 987
Size: 323 664
Bme1580I G_kGCm'C
Cuts at: 0 349 556 589 987
Size: 349 207 33 398
Fragments arranged by size:
398 349 207 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 402 987
Size: 402 585
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 405 462 987
Size: 405 57 525
Fragments arranged by size:
525 405 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 148 904 987
Size: 31 117 756 83
Fragments arranged by size:
756 117 83 31
Bpu10I CC'TnA_GC
Cuts at: 0 571 960 987
Size: 571 389 27
Fragments arranged by size:
571 389 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 987
Size: 7 980
BsaHI Gr'CG_yC
Cuts at: 0 560 665 879 987
Size: 560 105 214 108
Fragments arranged by size:
560 214 108 105
BsaWI w'CCGG_w
Cuts at: 0 274 987
Size: 274 713
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 937 967 987
Size: 937 30 20
Fragments arranged by size:
937 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 378 447 664 987
Size: 378 69 217 323
Fragments arranged by size:
378 323 217 69
BseYI C'CCAG_C
Cuts at: 0 134 549 760 936 987
Size: 134 415 211 176 51
Fragments arranged by size:
415 211 176 134 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 950 987
Size: 514 436 37
Fragments arranged by size:
514 436 37
Bsp1286I G_dGCh'C
Cuts at: 0 90 349 556 589 928 987
Size: 90 259 207 33 339 59
Fragments arranged by size:
339 259 207 90 59 33
BsrFI r'CCGG_y
Cuts at: 0 191 243 274 534 987
Size: 191 52 31 260 453
Fragments arranged by size:
453 260 191 52 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 68 987
Size: 68 919
BstYI r'GATC_y
Cuts at: 0 394 987
Size: 394 593
Bsu36I CC'TnA_GG
Cuts at: 0 390 987
Size: 390 597
BtgI C'CryG_G
Cuts at: 0 434 485 987
Size: 434 51 502
Fragments arranged by size:
502 434 51
EaeI y'GGCC_r
Cuts at: 0 189 987
Size: 189 798
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 90 205 987
Size: 90 115 782
Fragments arranged by size:
782 115 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 148 547 859 904 987
Size: 31 117 399 312 45 83
Fragments arranged by size:
399 312 117 83 45 31
EcoO109I rG'GnC_Cy
Cuts at: 0 184 346 423 594 987
Size: 184 162 77 171 393
Fragments arranged by size:
393 184 171 162 77
FseI GG_CCGG'CC
Cuts at: 0 538 987
Size: 538 449
FspI TGC'GCA
Cuts at: 0 625 987
Size: 625 362
HaeII r_GCGC'y
Cuts at: 0 761 882 987
Size: 761 121 105
Fragments arranged by size:
761 121 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 197 217 229 249 394 426 691 723
Size: 197 20 12 20 145 32 265 32
Cuts at: 723 987
Size: 264
Fragments arranged by size:
265 264 197 145 32 32 20 20
12
KasI G'GCGC_C
Cuts at: 0 878 987
Size: 878 109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 987
Size: 662 325
MslI CAynn'nnrTG
Cuts at: 0 773 987
Size: 773 214
MspA1I CmG'CkG
Cuts at: 0 166 360 518 525 570 605 964 987
Size: 166 194 158 7 45 35 359 23
Fragments arranged by size:
359 194 166 158 45 35 23 7
NaeI GCC'GGC
Cuts at: 0 193 245 536 987
Size: 193 52 291 451
Fragments arranged by size:
451 291 193 52
NarI GG'CG_CC
Cuts at: 0 879 987
Size: 879 108
NcoI C'CATG_G
Cuts at: 0 434 987
Size: 434 553
NgoMIV G'CCGG_C
Cuts at: 0 191 243 534 987
Size: 191 52 291 453
Fragments arranged by size:
453 291 191 52
NspI r_CATG'y
Cuts at: 0 52 987
Size: 52 935
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 760 987
Size: 315 445 227
Fragments arranged by size:
445 315 227
PfoI T'CCnGG_A
Cuts at: 0 143 803 987
Size: 143 660 184
Fragments arranged by size:
660 184 143
PspOMI G'GGCC_C
Cuts at: 0 345 552 987
Size: 345 207 435
Fragments arranged by size:
435 345 207
PvuII CAG'CTG
Cuts at: 0 360 525 570 605 964 987
Size: 360 165 45 35 359 23
Fragments arranged by size:
360 359 165 45 35 23
SfcI C'TryA_G
Cuts at: 0 600 987
Size: 600 387
SfoI GGC'GCC
Cuts at: 0 880 987
Size: 880 107
SgrAI Cr'CCGG_yG
Cuts at: 0 274 987
Size: 274 713
SmaI CCC'GGG
Cuts at: 0 268 371 987
Size: 268 103 616
Fragments arranged by size:
616 268 103
StuI AGG'CCT
Cuts at: 0 337 385 987
Size: 337 48 602
Fragments arranged by size:
602 337 48
StyI C'CwwG_G
Cuts at: 0 434 581 789 886 975 987
Size: 434 147 208 97 89 12
Fragments arranged by size:
434 208 147 97 89 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 604 987
Size: 604 383
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 937 969 987
Size: 937 32 18
Fragments arranged by size:
937 32 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 423 987
Size: 357 66 564
Fragments arranged by size:
564 357 66
XmaI C'CCGG_G
Cuts at: 0 266 369 987
Size: 266 103 618
Fragments arranged by size:
618 266 103
Enzymes that do cut:
AcuI AgeI AhdI AleI AlwNI ApaI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI Bsp1286I BsrFI BstAPI BstYI
Bsu36I BtgI EaeI EciI Eco57MI EcoO109I FseI FspI
HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PflMI PfoI PspOMI PvuII SfcI
SfoI SgrAI SmaI StuI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AlfI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BpuEI
BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV
FalI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI