(Circular) (Six-base) MAPSORT of: pFN21AB7842.seq Check: 5876 from: 1 to: 6556 pFN21AB7842 6556 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4366 278 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3899 1937 Size: 1962 4594 Acc65I G'GTAC_C Cuts at: 3877 3877 Size: 6556 AclI AA'CG_TT Cuts at: 1563 4683 5056 1563 Size: 3120 373 3063 Fragments arranged by size: 3120 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2076 2176 2879 3196 4619 6003 6271 1632 Size: 444 100 703 317 1423 1384 268 1917 Fragments arranged by size: 1917 1423 1384 703 444 317 268 100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6359 AflIII A'CryG_T Cuts at: 1203 2600 3029 5455 6432 1203 Size: 1397 429 2426 977 1327 Fragments arranged by size: 2426 1397 1327 977 429 AgeI A'CCGG_T Cuts at: 5387 5387 Size: 6556 AhdI GACnn_n'nnGTC Cuts at: 5285 5285 Size: 6556 AleI CACnn'nnGTG Cuts at: 1166 3423 1166 Size: 2257 4299 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6556 AlwNI CAG_nnn'CTG Cuts at: 4299 5871 4299 Size: 1572 4984 ApaLI G'TGCA_C Cuts at: 4613 5769 4613 Size: 1156 5400 ApoI r'AATT_y Cuts at: 1493 2048 2299 2705 3304 3641 3806 3865 4091 Size: 555 251 406 599 337 165 59 226 Cuts at: 4091 4243 6352 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 599 555 406 337 251 226 165 152 59 AseI AT'TA_AT Cuts at: 160 1032 5110 160 Size: 872 4078 1606 Fragments arranged by size: 4078 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 AvaI C'yCGr_G Cuts at: 1870 1943 3881 1870 Size: 73 1938 4545 Fragments arranged by size: 4545 1938 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3622 3655 3622 Size: 33 6523 BamHI G'GATC_C Cuts at: 3886 4253 3886 Size: 367 6189 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3877 5332 618 Size: 325 217 627 2090 1455 1842 Fragments arranged by size: 2090 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2720 3875 1391 Size: 1329 1155 4072 Fragments arranged by size: 4072 1329 1155 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2110 2282 3212 928 Size: 971 211 172 930 4272 Fragments arranged by size: 4272 971 930 211 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4747 4781 1903 Size: 34 2810 34 3678 Fragments arranged by size: 3678 2810 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4448 5658 4448 Size: 1210 5346 BclI T'GATC_A Cuts at: 1548 1548 Size: 6556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5167 136 Size: 107 122 71 631 605 3495 1525 Fragments arranged by size: 3495 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3795 6551 3795 Size: 2756 3800 BlpI GC'TnA_GC Cuts at: 3973 3973 Size: 6556 Bme1580I G_kGCm'C Cuts at: 1790 4617 5773 1790 Size: 2827 1156 2573 Fragments arranged by size: 2827 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6556 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2928 4287 5245 Size: 416 53 83 399 24 1511 1359 958 Cuts at: 5245 442 Size: 1753 Fragments arranged by size: 1753 1511 1359 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6556 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3567 3817 3567 Size: 250 6306 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2772 3612 3801 5216 1239 Size: 1533 840 189 1415 2579 Fragments arranged by size: 2579 1533 1415 840 189 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3419 4681 5546 5844 3419 Size: 1262 865 298 4131 Fragments arranged by size: 4131 1262 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2415 3424 5219 882 Size: 617 916 1009 1795 2219 Fragments arranged by size: 2219 1795 1009 916 617 BsaAI yAC'GTr Cuts at: 493 1204 2497 4347 493 Size: 711 1293 1850 2702 Fragments arranged by size: 2702 1850 1293 711 BsaBI GATnn'nnATC Cuts at: 2565 6550 2565 Size: 3985 2571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4363 4745 5380 275 Size: 53 83 186 936 2830 382 635 1451 Fragments arranged by size: 2830 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4989 5387 5661 5808 6219 4989 Size: 398 274 147 411 5326 Fragments arranged by size: 5326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6331 Fragments arranged by size: 6331 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2144 3377 1186 Size: 955 3 1233 4365 Fragments arranged by size: 4365 1233 955 3 BseYI C'CCAG_C Cuts at: 2134 2635 3468 3720 5759 6255 2134 Size: 501 833 252 2039 496 2435 Fragments arranged by size: 2435 2039 833 501 496 252 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5983 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4006 4767 5795 1540 Size: 275 186 2005 761 1028 2301 Fragments arranged by size: 2301 2005 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2720 4617 4702 5773 2720 Size: 1897 85 1071 3503 Fragments arranged by size: 3503 1897 1071 85 BsmI GAATG_Cn' Cuts at: 2986 4067 4160 2986 Size: 1081 93 5382 Fragments arranged by size: 5382 1081 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2720 3875 4617 4702 5773 1391 Size: 399 930 1155 742 85 1071 2174 Fragments arranged by size: 2174 1155 1071 930 742 399 85 BspEI T'CCGG_A Cuts at: 6219 6219 Size: 6556 BspHI T'CATG_A Cuts at: 2881 4445 6175 2881 Size: 1564 1730 3262 Fragments arranged by size: 3262 1730 1564 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2208 3911 844 Size: 1364 1703 3489 Fragments arranged by size: 3489 1703 1364 BsrBI CCG'CTC Cuts at: 1394 1823 4443 1394 Size: 429 2620 3507 Fragments arranged by size: 3507 2620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2783 2818 3119 5051 5225 65 Size: 1183 1535 35 301 1932 174 1396 Fragments arranged by size: 1932 1535 1396 1183 301 174 35 BsrFI r'CCGG_y Cuts at: 1700 1810 5200 5387 1700 Size: 110 3390 187 2869 Fragments arranged by size: 3390 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6556 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6556 BssSI C'ACGA_G Cuts at: 4616 5628 4616 Size: 1012 5544 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6556 BstBI TT'CG_AA Cuts at: 5360 5360 Size: 6556 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6556 BstYI r'GATC_y Cuts at: 1972 2313 3329 3394 3795 3886 4253 4638 4655 Size: 341 1016 65 401 91 367 385 17 Cuts at: 4655 6107 6193 6205 6551 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1016 401 385 367 346 341 91 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2991 3075 513 Size: 552 1926 84 3994 Fragments arranged by size: 3994 1926 552 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5817 Fragments arranged by size: 5817 683 56 BtsI GCAGTG_nn' Cuts at: 2097 3623 4072 4866 4886 2097 Size: 1526 449 794 20 3767 Fragments arranged by size: 3767 1526 794 449 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6556 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6521 DraI TTT'AAA Cuts at: 3860 3860 Size: 6556 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5563 809 Size: 507 4247 1802 Fragments arranged by size: 4247 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4003 4892 6521 Size: 54 1425 50 275 18 2173 889 1629 Cuts at: 6521 8 Size: 43 Fragments arranged by size: 2173 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4003 1537 Size: 275 2191 4090 Fragments arranged by size: 4090 2191 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 3084 3525 3786 4486 1353 Size: 999 732 441 261 700 3423 Fragments arranged by size: 3423 999 732 700 441 261 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5143 5517 5663 387 Size: 1064 138 3554 374 146 1280 Fragments arranged by size: 3554 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2718 2718 Size: 6556 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 2176 2772 2879 3196 3612 3801 Size: 393 444 100 596 107 317 416 189 Cuts at: 3801 4619 5216 6003 6271 1239 Size: 818 597 787 268 1524 Fragments arranged by size: 1524 818 787 597 596 444 416 393 317 268 189 107 100 EcoRI G'AATT_C Cuts at: 2299 3806 3865 4243 2299 Size: 1507 59 378 4612 Fragments arranged by size: 4612 1507 378 59 FspI TGC'GCA Cuts at: 5062 5062 Size: 6556 HaeII r_GCGC'y Cuts at: 1678 5703 1678 Size: 4025 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2298 2330 2707 2739 3379 Size: 32 135 32 726 32 377 32 640 Cuts at: 3379 3411 5197 5229 5271 5303 1373 Size: 32 1786 32 42 32 2626 Fragments arranged by size: 2626 1786 726 640 377 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3900 4146 669 Size: 988 281 1962 246 3079 Fragments arranged by size: 3079 1962 988 281 246 HindIII A'AGCT_T Cuts at: 748 2620 748 Size: 1872 4684 HpaI GTT'AAC Cuts at: 4146 4146 Size: 6556 KpnI G_GTAC'C Cuts at: 3881 3881 Size: 6556 MfeI C'AATT_G Cuts at: 4155 4155 Size: 6556 MluI A'CGCG_T Cuts at: 6432 6432 Size: 6556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3372 5670 5854 1305 Size: 2067 2298 184 2007 Fragments arranged by size: 2298 2067 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4734 Fragments arranged by size: 4734 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2601 2935 3019 3103 3423 4514 4873 Size: 648 1435 334 84 84 320 1091 359 Cuts at: 4873 5032 518 Size: 159 2042 Fragments arranged by size: 2042 1435 1091 648 359 334 320 159 84 84 MspA1I CmG'CkG Cuts at: 1606 3972 4649 5797 6042 1606 Size: 2366 677 1148 245 2120 Fragments arranged by size: 2366 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2991 3075 513 Size: 552 1926 84 3994 Fragments arranged by size: 3994 1926 552 84 NdeI CA'TA_TG Cuts at: 387 2664 387 Size: 2277 4279 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6556 NotI GC'GGCC_GC Cuts at: 4003 4003 Size: 6556 NspI r_CATG'y Cuts at: 1132 1229 2604 3033 3585 3658 3914 5459 1132 Size: 97 1375 429 552 73 256 1545 2229 Fragments arranged by size: 2229 1545 1375 552 429 256 97 73 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6556 PciI A'CATG_T Cuts at: 2600 3029 5455 2600 Size: 429 2426 3701 Fragments arranged by size: 3701 2426 429 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6556 PmeI GTTT'AAAC Cuts at: 3860 3860 Size: 6556 PmlI CAC'GTG Cuts at: 2497 2497 Size: 6556 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2189 2221 4625 4657 6138 6170 2189 Size: 32 2404 32 1481 32 2575 Fragments arranged by size: 2575 2404 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6556 PsiI TTA'TAA Cuts at: 2850 4126 2850 Size: 1276 5280 PstI C_TGCA'G Cuts at: 830 3908 830 Size: 3078 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6556 SacI G_AGCT'C Cuts at: 2720 2720 Size: 6556 SalI G'TCGA_C Cuts at: 1936 3898 1936 Size: 1962 4594 SbfI CC_TGCA'GG Cuts at: 3908 3908 Size: 6556 ScaI AGT'ACT Cuts at: 4804 6516 4804 Size: 1712 4844 SexAI A'CCwGG_T Cuts at: 2405 2405 Size: 6556 SfcI C'TryA_G Cuts at: 826 1045 3904 5039 5720 5911 826 Size: 219 2859 1135 681 191 1471 Fragments arranged by size: 2859 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6556 SmaI CCC'GGG Cuts at: 1872 3883 1872 Size: 2011 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3398 4660 5561 5823 820 Size: 197 926 1455 1262 901 262 1553 Fragments arranged by size: 1553 1455 1262 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4347 493 Size: 3854 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6556 SphI G_CATG'C Cuts at: 1132 3914 1132 Size: 2782 3774 SspI AAT'ATT Cuts at: 5 52 4480 5 Size: 47 4428 2081 Fragments arranged by size: 4428 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6556 StyI C'CwwG_G Cuts at: 513 1065 2991 3075 3995 513 Size: 552 1926 84 920 3074 Fragments arranged by size: 3074 1926 920 552 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4583 4600 4753 4938 5383 325 Size: 1255 3003 17 153 185 445 1498 Fragments arranged by size: 3003 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3557 4802 6514 Size: 274 80 33 51 1443 1580 1245 1712 Cuts at: 6514 96 Size: 138 Fragments arranged by size: 1712 1580 1443 1245 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6516 6548 6516 Size: 32 6524 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6556 XbaI T'CTAG_A Cuts at: 3892 3892 Size: 6556 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2358 2358 Size: 6556 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6556 XmaI C'CCGG_G Cuts at: 1870 3881 1870 Size: 2011 4545 XmnI GAAnn'nnTTC Cuts at: 2028 2762 2789 2873 2930 3209 4685 2028 Size: 734 27 84 57 279 1476 3899 Fragments arranged by size: 3899 1476 734 279 84 57 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4364 276 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI