Restriction map for
pFN21AB7809
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AciI 217 GCGG CCGC(-3/-1)
AlwNI 358 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 290 TGGCCA TGG^CCA
BamHI 1061 GGATCC G^GATCC
BbsI 152 GTCTTC GAAGAC(2/6)
BglII 1326 AGATCT A^GATCT
BpuAI 152 GTCTTC GAAGAC(2/6)
BseRI 64 GAGGAG GAGGAG(10/8)
BsmAI 310 GAGAC GTCTC(1/5)
BspHI 756 TCATGA T^CATGA
BspMI 246 ACCTGC ACCTGC(4/8)
BsrDI 923 GCAATG GCAATG(2/0)
BstAPI 244 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 98 CATCC GGATG(2/0)
CfoI 623 GCGC GCG^C
EaeI 290 TGGCCA [CT]^GGCC[GA]
EcoNI 448 CCTGAGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 98 CATCC GGATG(9/13)
HaeII 622 GGCGCT [GA]GCGC^[CT]
HhaI 623 GCGC GCG^C
HinP1I 623 GCGC G^CGC
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
MwoI 244 GCACCTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 1166 CATATG CA^TATG
PpuMI 444 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1451 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 756 TCATGA T^CATGA
SacI 1035 GAGCTC GAGCT^C
SmlI 440 CTCAAG C^T[CT][GA]AG
TaqI 1459 TCGA T^CGA
Tsp45I 545 GTCAC ^GT[GC]AC
TthHB8I 1459 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 883 GTAC GT^AC
1135 GTAC
ApaI 42 GGGCCC GGGCC^C
137 GGGCCC
AvaII 320 GGACC G^G[AT]CC
445 GGACC
BanI 243 GGCACC G^G[CT][GA]CC
968 GGCACC
BsaAI 666 TACGTG [CT]AC^GT[GA]
1336 TACGTG
BsgI 145 GTGCAG GTGCAG(16/14)
1523 GTGCAG
BsmFI 283 GGGAC GGGAC(10/14)
319 GGGAC
Bsp1407I 882 TGTACA T^GTACA
1134 TGTACA
BsrGI 882 TGTACA T^GTACA
1134 TGTACA
BstYI 1061 GGATCC [GA]^GATC[CT]
1326 AGATCT
DsaI 23 CCATGG C^C[GA][CT]GG
37 CCATGG
EarI 178 GAAGAG CTCTTC(1/4)
568 GAAGAG
HindIII 461 AAGCTT A^AGCTT
558 AAGCTT
Ksp632I 178 GAAGAG CTCTTC(1/4)
568 GAAGAG
MaeII 667 ACGT A^CGT
1337 ACGT
MflI 1061 GGATCC [GA]^GATC[CT]
1326 AGATCT
NcoI 23 CCATGG C^CATGG
37 CCATGG
PleI 285 GACTC GAGTC(4/5)
312 GACTC
RsaI 883 GTAC GT^AC
1135 GTAC
SspBI 882 TGTACA T^GTACA
1134 TGTACA
XhoII 1061 GGATCC [GA]^GATC[CT]
1326 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AflIII AluI Alw44I AlwI
ApaLI ApoI Asp700I AspHI BanII
BbvI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BspLU11I
BsrI BssKI BstNI Cfr13I DdeI
DpnII DraII EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII NlaIV NspI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TfiI Tru9I TseI Tsp509I
TspRI XmnI