Restriction map for
pFN21AB7809
(Circular) (Six-base) MAPSORT of: pFN21AB7809.seq Check: 9202 from: 1 to: 6286
pFN21AB7809 6286 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2260 2260
Size: 6286
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3629 1937
Size: 1692 4594
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 3268 3436 4349 5733 6001 1632
Size: 512 1124 168 913 1384 268 1917
Fragments arranged by size:
1917 1384 1124 913 512 268 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 2484 2672 3342 3371 3428 5185 6162 1203
Size: 1281 188 670 29 57 1757 977 1327
Fragments arranged by size:
1757 1327 1281 977 670 188 57 29
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 5015 5015
Size: 6286
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6286
AlwNI CAG_nnn'CTG
Cuts at: 2368 4029 5601 2368
Size: 1661 1572 3053
Fragments arranged by size:
3053 1661 1572
ApaI G_GGCC'C
Cuts at: 2051 2146 2051
Size: 95 6191
ApaLI G'TGCA_C
Cuts at: 2117 3224 3476 4343 5499 2117
Size: 1107 252 867 1156 2904
Fragments arranged by size:
2904 1156 1107 867 252
ApoI r'AATT_y
Cuts at: 1493 3234 3318 3402 3595 3821 3973 6082 1493
Size: 1741 84 84 193 226 152 2109 1697
Fragments arranged by size:
2109 1741 1697 226 193 152 84 84
AseI AT'TA_AT
Cuts at: 160 1032 4840 160
Size: 872 3808 1606
Fragments arranged by size:
3808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 3611 1870
Size: 73 1668 4545
Fragments arranged by size:
4545 1668 73
BamHI G'GATC_C
Cuts at: 3066 3616 3983 3066
Size: 550 367 5369
Fragments arranged by size:
5369 550 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2248 2973 3607 5062 618
Size: 325 217 627 461 725 634 1455 1842
Fragments arranged by size:
1842 1455 725 634 627 461 325 217
BanII G_rGCy'C
Cuts at: 1391 2051 2146 3044 3605 1391
Size: 660 95 898 561 4072
Fragments arranged by size:
4072 898 660 561 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 928
Size: 971 251 5064
Fragments arranged by size:
5064 971 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4477 4511 1903
Size: 34 2540 34 3678
Fragments arranged by size:
3678 2540 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4897 136
Size: 107 122 71 631 605 3225 1525
Fragments arranged by size:
3225 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3331 6281 3331
Size: 2950 3336
BlpI GC'TnA_GC
Cuts at: 3703 3703
Size: 6286
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2121 2146 3228 3480 4347 5503 1790
Size: 261 70 25 1082 252 867 1156 2573
Fragments arranged by size:
2573 1156 1082 867 261 252 70 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2045 2141 3084
Size: 416 53 83 399 24 628 96 943
Cuts at: 3084 3504 4017 4975 442
Size: 420 513 958 1753
Fragments arranged by size:
1753 958 943 628 513 420 416 399
96 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2622 2760 4946 1239
Size: 1094 289 138 2186 2579
Fragments arranged by size:
2579 2186 1094 289 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2430 4411 5276 5574 2430
Size: 1981 865 298 3142
Fragments arranged by size:
3142 1981 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4949 882
Size: 617 3450 2219
Fragments arranged by size:
3450 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2673 3343 4077 493
Size: 711 1469 670 734 2702
Fragments arranged by size:
2702 1469 734 711 670
BsaBI GATnn'nnATC
Cuts at: 6280 6280
Size: 6286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4093 4475 5110 275
Size: 53 83 186 936 2560 382 635 1451
Fragments arranged by size:
2560 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6061
Fragments arranged by size:
6061 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 1186
Size: 898 5388
BseYI C'CCAG_C
Cuts at: 2649 5489 5985 2649
Size: 2840 496 2950
Fragments arranged by size:
2950 2840 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 3549 1163
Size: 573 435 1378 3900
Fragments arranged by size:
3900 1378 573 435
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 3044 3228 3480 4347 4432 5503 2121
Size: 923 184 252 867 85 1071 2904
Fragments arranged by size:
2904 1071 923 867 252 184 85
BsmI GAATG_Cn'
Cuts at: 3797 3890 3797
Size: 93 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2121 2146 3044 3228 3480 3605
Size: 399 261 70 25 898 184 252 125
Cuts at: 3605 4347 4432 5503 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 898 742 399 261 252 184
125 85 70 25
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 2761 4175 5905 2761
Size: 1414 1730 3142
Fragments arranged by size:
3142 1730 1414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2260 3641 844
Size: 1416 1381 3489
Fragments arranged by size:
3489 1416 1381
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2935 4781 4955 65
Size: 1183 1687 1846 174 1396
Fragments arranged by size:
1846 1687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4930 5117 1700
Size: 110 3120 187 2869
Fragments arranged by size:
3120 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2887 3139 96
Size: 2791 252 3243
Fragments arranged by size:
3243 2791 252
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 4346 5358 4346
Size: 1012 5274
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2255 794
Size: 1461 4825
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 3066 3331 3616 3983 4368 4385 5837 5923
Size: 1094 265 285 367 385 17 1452 86
Cuts at: 5923 5935 6281 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1094 385 367 346 285 265
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2028 2042 513
Size: 552 963 14 4757
Fragments arranged by size:
4757 963 552 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5547
Fragments arranged by size:
5547 683 56
BtsI GCAGTG_nn'
Cuts at: 3240 3802 4596 4616 3240
Size: 562 794 20 4910
Fragments arranged by size:
4910 794 562 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 3733 4622
Size: 54 1425 50 275 18 465 1438 889
Cuts at: 4622 6251 8
Size: 1629 43
Fragments arranged by size:
1629 1438 1425 889 465 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2568 4216 1353
Size: 825 390 1648 3423
Fragments arranged by size:
3423 1648 825 390
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3042 3042
Size: 6286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2333 2622 2760 3268 3436 4349
Size: 393 512 189 289 138 508 168 913
Cuts at: 4349 4946 5733 6001 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 913 787 597 512 508 393 289
268 189 168 138
EcoNI CCTnn'n_nnAGG
Cuts at: 2457 2457
Size: 6286
EcoO109I rG'GnC_Cy
Cuts at: 2047 2143 2450 2047
Size: 96 307 5883
Fragments arranged by size:
5883 307 96
EcoRI G'AATT_C
Cuts at: 3234 3318 3402 3595 3973 3234
Size: 84 84 193 378 5547
Fragments arranged by size:
5547 378 193 84 84
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 2631 5433 1678
Size: 953 2802 2531
Fragments arranged by size:
2802 2531 953
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2863 2875 2895 2907 3115
Size: 32 135 32 1291 12 20 12 208
Cuts at: 3115 3147 4927 4959 5001 5033 1373
Size: 32 1780 32 42 32 2626
Fragments arranged by size:
2626 1780 1291 208 135 42 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3630 3876 669
Size: 988 281 1692 246 3079
Fragments arranged by size:
3079 1692 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2466 2563 748
Size: 1718 97 4471
Fragments arranged by size:
4471 1718 97
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3567 5400 5584 1305
Size: 2262 1833 184 2007
Fragments arranged by size:
2262 2007 1833 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 10
Size: 54 1425 343 465 3999
Fragments arranged by size:
3999 1425 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2766 3159 3175 4244 4603 4762 518
Size: 648 1600 393 16 1069 359 159 2042
Fragments arranged by size:
2042 1600 1069 648 393 359 159 16
MspA1I CmG'CkG
Cuts at: 1606 3702 4379 5527 5772 1606
Size: 2096 677 1148 245 2120
Fragments arranged by size:
2120 2096 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2028 2042 513
Size: 552 963 14 4757
Fragments arranged by size:
4757 963 552 14
NdeI CA'TA_TG
Cuts at: 387 3172 387
Size: 2785 3501
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2488 3375 3432 3644 5189 1132
Size: 97 1259 887 57 212 1545 2229
Fragments arranged by size:
2229 1545 1259 887 212 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 2484 3371 3428 5185 2484
Size: 887 57 1757 3585
Fragments arranged by size:
3585 1757 887 57
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6286
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2450 2450
Size: 6286
PshAI GACnn'nnGTC
Cuts at: 1613 3460 1613
Size: 1847 4439
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PspOMI G'GGCC_C
Cuts at: 2047 2142 2047
Size: 95 6191
PstI C_TGCA'G
Cuts at: 830 2014 3247 3266 3638 830
Size: 1184 1233 19 372 3478
Fragments arranged by size:
3478 1233 1184 372 19
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
SacI G_AGCT'C
Cuts at: 3044 3044
Size: 6286
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 4534 6246 4534
Size: 1712 4574
SfcI C'TryA_G
Cuts at: 826 1045 2010 2360 3243 3262 3634 4769 5450
Size: 219 965 350 883 19 372 1135 681
Cuts at: 5450 5641 826
Size: 191 1471
Fragments arranged by size:
1471 1135 965 883 681 372 350 219
191 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2445 4390 5291 5553 820
Size: 197 926 502 1945 901 262 1553
Fragments arranged by size:
1945 1553 926 901 502 262 197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3644 1132
Size: 2512 3774
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 2169 2777 3365 3449 1517
Size: 652 608 588 84 4354
Fragments arranged by size:
4354 652 608 588 84
StyI C'CwwG_G
Cuts at: 513 1065 2028 2042 2266 3725 513
Size: 552 963 14 224 1459 3074
Fragments arranged by size:
3074 1459 963 552 224 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4313 4330 4483 4668 5113 325
Size: 1255 2733 17 153 185 445 1498
Fragments arranged by size:
2733 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2887 3139 4532
Size: 274 80 33 51 1443 910 252 1393
Cuts at: 4532 6244 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1393 910 274 252 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6246 6278 6246
Size: 32 6254
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6286
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 2522 2777 2861 3281 3365 3449 4415 2522
Size: 255 84 420 84 84 966 4393
Fragments arranged by size:
4393 966 420 255 84 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI