Restriction map for
pFN21AB7809
(Linear) (Six-base) MAPSORT of: KIBB7809.seq Check: 3232 from: 1 to: 1581
KIBB7809.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 255 1581
Size: 255 1326
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1263 1431 1581
Size: 139 1124 168 150
Fragments arranged by size:
1124 168 150 139
AflIII A'CryG_T
Cuts at: 0 479 667 1337 1366 1423 1581
Size: 479 188 670 29 57 158
Fragments arranged by size:
670 479 188 158 57 29
AlwNI CAG_nnn'CTG
Cuts at: 0 363 1581
Size: 363 1218
ApaI G_GGCC'C
Cuts at: 0 46 141 1581
Size: 46 95 1440
Fragments arranged by size:
1440 95 46
ApaLI G'TGCA_C
Cuts at: 0 112 1219 1471 1581
Size: 112 1107 252 110
Fragments arranged by size:
1107 252 112 110
ApoI r'AATT_y
Cuts at: 0 1229 1313 1397 1581
Size: 1229 84 84 184
Fragments arranged by size:
1229 184 84 84
BamHI G'GATC_C
Cuts at: 0 1061 1581
Size: 1061 520
BanI G'GyrC_C
Cuts at: 0 243 968 1581
Size: 243 725 613
Fragments arranged by size:
725 613 243
BanII G_rGCy'C
Cuts at: 0 46 141 1039 1581
Size: 46 95 898 542
Fragments arranged by size:
898 542 95 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 1581
Size: 145 1436
BglII A'GATC_T
Cuts at: 0 1326 1581
Size: 1326 255
Bme1580I G_kGCm'C
Cuts at: 0 46 116 141 1223 1475 1581
Size: 46 70 25 1082 252 106
Fragments arranged by size:
1082 252 106 70 46 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 40 136 1079 1499 1581
Size: 40 96 943 420 82
Fragments arranged by size:
943 420 96 82 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 617 755 1581
Size: 328 289 138 826
Fragments arranged by size:
826 328 289 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 1581
Size: 425 1156
BsaAI yAC'GTr
Cuts at: 0 668 1338 1581
Size: 668 670 243
Fragments arranged by size:
670 668 243
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 1581
Size: 79 1502
BseYI C'CCAG_C
Cuts at: 0 644 1581
Size: 644 937
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1544 1581
Size: 166 1378 37
Fragments arranged by size:
1378 166 37
BsiHKAI G_wGCw'C
Cuts at: 0 116 1039 1223 1475 1581
Size: 116 923 184 252 106
Fragments arranged by size:
923 252 184 116 106
Bsp1286I G_dGCh'C
Cuts at: 0 46 116 141 1039 1223 1475 1581
Size: 46 70 25 898 184 252 106
Fragments arranged by size:
898 252 184 106 70 46 25
BspHI T'CATG_A
Cuts at: 0 756 1581
Size: 756 825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 255 1581
Size: 255 1326
BsrDI GCAATG_nn'
Cuts at: 0 930 1581
Size: 930 651
BsrGI T'GTAC_A
Cuts at: 0 882 1134 1581
Size: 882 252 447
Fragments arranged by size:
882 447 252
BstAPI GCAn_nnn'nTGC
Cuts at: 0 250 1581
Size: 250 1331
BstYI r'GATC_y
Cuts at: 0 1061 1326 1581
Size: 1061 265 255
Fragments arranged by size:
1061 265 255
BtgI C'CryG_G
Cuts at: 0 23 37 1581
Size: 23 14 1544
Fragments arranged by size:
1544 23 14
BtsI GCAGTG_nn'
Cuts at: 0 1235 1581
Size: 1235 346
EaeI y'GGCC_r
Cuts at: 0 290 1581
Size: 290 1291
EarI CTCTTCn'nnn_
Cuts at: 0 173 563 1581
Size: 173 390 1018
Fragments arranged by size:
1018 390 173
EcoICRI GAG'CTC
Cuts at: 0 1037 1581
Size: 1037 544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 328 617 755 1263 1431 1581
Size: 139 189 289 138 508 168 150
Fragments arranged by size:
508 289 189 168 150 139 138
EcoNI CCTnn'n_nnAGG
Cuts at: 0 452 1581
Size: 452 1129
EcoO109I rG'GnC_Cy
Cuts at: 0 42 138 445 1581
Size: 42 96 307 1136
Fragments arranged by size:
1136 307 96 42
EcoRI G'AATT_C
Cuts at: 0 1229 1313 1397 1581
Size: 1229 84 84 184
Fragments arranged by size:
1229 184 84 84
HaeII r_GCGC'y
Cuts at: 0 626 1581
Size: 626 955
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 858 870 890 902 1110 1142 1581
Size: 858 12 20 12 208 32 439
Fragments arranged by size:
858 439 208 32 20 12 12
HindIII A'AGCT_T
Cuts at: 0 461 558 1581
Size: 461 97 1023
Fragments arranged by size:
1023 461 97
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1562 1581
Size: 1562 19
MscI TGG'CCA
Cuts at: 0 292 1581
Size: 292 1289
MslI CAynn'nnrTG
Cuts at: 0 761 1154 1170 1581
Size: 761 393 16 411
Fragments arranged by size:
761 411 393 16
NcoI C'CATG_G
Cuts at: 0 23 37 1581
Size: 23 14 1544
Fragments arranged by size:
1544 23 14
NdeI CA'TA_TG
Cuts at: 0 1167 1581
Size: 1167 414
NspI r_CATG'y
Cuts at: 0 483 1370 1427 1581
Size: 483 887 57 154
Fragments arranged by size:
887 483 154 57
PciI A'CATG_T
Cuts at: 0 479 1366 1423 1581
Size: 479 887 57 158
Fragments arranged by size:
887 479 158 57
PpuMI rG'GwC_Cy
Cuts at: 0 445 1581
Size: 445 1136
PshAI GACnn'nnGTC
Cuts at: 0 1455 1581
Size: 1455 126
PspOMI G'GGCC_C
Cuts at: 0 42 137 1581
Size: 42 95 1444
Fragments arranged by size:
1444 95 42
PstI C_TGCA'G
Cuts at: 0 9 1242 1261 1581
Size: 9 1233 19 320
Fragments arranged by size:
1233 320 19 9
SacI G_AGCT'C
Cuts at: 0 1039 1581
Size: 1039 542
SfcI C'TryA_G
Cuts at: 0 5 355 1238 1257 1581
Size: 5 350 883 19 324
Fragments arranged by size:
883 350 324 19 5
SmlI C'TyrA_G
Cuts at: 0 440 1581
Size: 440 1141
StuI AGG'CCT
Cuts at: 0 164 772 1360 1444 1581
Size: 164 608 588 84 137
Fragments arranged by size:
608 588 164 137 84
StyI C'CwwG_G
Cuts at: 0 23 37 261 1581
Size: 23 14 224 1320
Fragments arranged by size:
1320 224 23 14
TatI w'GTAC_w
Cuts at: 0 882 1134 1581
Size: 882 252 447
Fragments arranged by size:
882 447 252
XmnI GAAnn'nnTTC
Cuts at: 0 517 772 856 1276 1360 1444 1581
Size: 517 255 84 420 84 84 137
Fragments arranged by size:
517 420 255 137 84 84 84
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApaLI ApoI BamHI
BanI BanII BbsI BglII Bme1580I BmrI BpmI BpuEI
BsaAI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BsrGI BstAPI BstYI BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HindIII
MmeI MscI MslI NcoI NdeI NspI PciI PpuMI
PshAI PspOMI PstI SacI SfcI SmlI StuI StyI
TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI ZraI