(Circular) (Six-base) MAPSORT of: pFN21AB7774.seq Check: 6443 from: 1 to: 6223 pFN21AB7774 6223 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4033 278 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3566 1937 Size: 1629 4594 Acc65I G'GTAC_C Cuts at: 3544 3544 Size: 6223 AclI AA'CG_TT Cuts at: 1563 4350 4723 1563 Size: 2787 373 3063 Fragments arranged by size: 3063 2787 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2584 3256 4286 5670 5938 1632 Size: 952 672 1030 1384 268 1917 Fragments arranged by size: 1917 1384 1030 952 672 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6026 AflIII A'CryG_T Cuts at: 1203 5122 6099 1203 Size: 3919 977 1327 Fragments arranged by size: 3919 1327 977 AgeI A'CCGG_T Cuts at: 5054 5054 Size: 6223 AhdI GACnn_n'nnGTC Cuts at: 4952 4952 Size: 6223 AleI CACnn'nnGTG Cuts at: 1166 2314 2979 3063 1166 Size: 1148 665 84 4326 Fragments arranged by size: 4326 1148 665 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6223 AlwNI CAG_nnn'CTG Cuts at: 3966 5538 3966 Size: 1572 4651 ApaLI G'TGCA_C Cuts at: 3110 4280 5436 3110 Size: 1170 1156 3897 Fragments arranged by size: 3897 1170 1156 ApoI r'AATT_y Cuts at: 1493 2970 3014 3138 3390 3532 3758 3910 6019 Size: 1477 44 124 252 142 226 152 2109 Cuts at: 6019 1493 Size: 1697 Fragments arranged by size: 2109 1697 1477 252 226 152 142 124 44 AseI AT'TA_AT Cuts at: 160 1032 4777 160 Size: 872 3745 1606 Fragments arranged by size: 3745 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 AvaI C'yCGr_G Cuts at: 1870 1943 3548 1870 Size: 73 1605 4545 Fragments arranged by size: 4545 1605 73 BamHI G'GATC_C Cuts at: 3126 3553 3920 3126 Size: 427 367 5429 Fragments arranged by size: 5429 427 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3544 4999 618 Size: 325 217 627 1757 1455 1842 Fragments arranged by size: 1842 1757 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3542 1391 Size: 2151 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5252 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4414 4448 1903 Size: 34 50 34 2393 34 3678 Fragments arranged by size: 3678 2393 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4115 5325 4115 Size: 1210 5013 BclI T'GATC_A Cuts at: 1548 1548 Size: 6223 BfrBI ATG'CAT Cuts at: 3226 3226 Size: 6223 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4834 136 Size: 107 122 71 631 605 3162 1525 Fragments arranged by size: 3162 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2066 6218 2066 Size: 4152 2071 BlpI GC'TnA_GC Cuts at: 3640 3640 Size: 6223 Bme1580I G_kGCm'C Cuts at: 1790 3114 4284 5440 1790 Size: 1324 1170 1156 2573 Fragments arranged by size: 2573 1324 1170 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6223 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3954 4912 442 Size: 416 53 83 399 24 2537 958 1753 Fragments arranged by size: 2537 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6223 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2296 2296 Size: 6223 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2748 4883 1239 Size: 956 553 2135 2579 Fragments arranged by size: 2579 2135 956 553 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4348 5213 5511 4348 Size: 865 298 5060 Fragments arranged by size: 5060 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2171 2560 2728 3400 4886 882 Size: 617 672 389 168 672 1486 2219 Fragments arranged by size: 2219 1486 672 672 617 389 168 BsaAI yAC'GTr Cuts at: 493 1204 4014 493 Size: 711 2810 2702 Fragments arranged by size: 2810 2702 711 BsaBI GATnn'nnATC Cuts at: 6217 6217 Size: 6223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4030 4412 5047 275 Size: 53 83 186 936 2497 382 635 1451 Fragments arranged by size: 2497 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3300 4656 5054 5328 5475 5886 3300 Size: 1356 398 274 147 411 3637 Fragments arranged by size: 3637 1356 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5998 Fragments arranged by size: 5998 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2262 2285 2490 1186 Size: 1076 23 205 4919 Fragments arranged by size: 4919 1076 205 23 BseYI C'CCAG_C Cuts at: 5426 5922 5426 Size: 496 5727 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5650 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3673 4434 5462 1540 Size: 275 186 1672 761 1028 2301 Fragments arranged by size: 2301 1672 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3114 4284 4369 5440 3114 Size: 1170 85 1071 3897 Fragments arranged by size: 3897 1170 1071 85 BsmI GAATG_Cn' Cuts at: 2582 3226 3734 3827 2582 Size: 644 508 93 4978 Fragments arranged by size: 4978 644 508 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3114 3542 4284 4369 5440 1391 Size: 399 1324 428 742 85 1071 2174 Fragments arranged by size: 2174 1324 1071 742 428 399 85 BspEI T'CCGG_A Cuts at: 5886 5886 Size: 6223 BspHI T'CATG_A Cuts at: 2497 4112 5842 2497 Size: 1615 1730 2878 Fragments arranged by size: 2878 1730 1615 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3578 844 Size: 2734 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4110 1394 Size: 429 2287 3507 Fragments arranged by size: 3507 2287 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2329 2619 4718 4892 65 Size: 1183 1081 290 2099 174 1396 Fragments arranged by size: 2099 1396 1183 1081 290 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4867 5054 1700 Size: 110 3057 187 2869 Fragments arranged by size: 3057 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3500 96 Size: 3404 2819 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6223 BssSI C'ACGA_G Cuts at: 4283 5295 4283 Size: 1012 5211 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6223 BstBI TT'CG_AA Cuts at: 5027 5027 Size: 6223 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6223 BstYI r'GATC_y Cuts at: 1972 2066 2300 3126 3553 3920 4305 4322 5774 Size: 94 234 826 427 367 385 17 1452 Cuts at: 5774 5860 5872 6218 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 826 427 385 367 346 234 94 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2281 2348 2281 Size: 67 6156 BtgI C'CryG_G Cuts at: 513 1065 2287 513 Size: 552 1222 4449 Fragments arranged by size: 4449 1222 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5484 Fragments arranged by size: 5484 683 56 BtsI GCAGTG_nn' Cuts at: 2309 3739 4533 4553 2309 Size: 1430 794 20 3979 Fragments arranged by size: 3979 1430 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6223 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6188 DraI TTT'AAA Cuts at: 3527 3527 Size: 6223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5230 809 Size: 507 3914 1802 Fragments arranged by size: 3914 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 3670 4559 Size: 54 1425 50 275 18 384 1456 889 Cuts at: 4559 6188 8 Size: 1629 43 Fragments arranged by size: 1629 1456 1425 889 384 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3670 1537 Size: 275 1858 4090 Fragments arranged by size: 4090 1858 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2247 4153 1353 Size: 894 1906 3423 Fragments arranged by size: 3423 1906 894 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4810 5184 5330 387 Size: 1064 138 3221 374 146 1280 Fragments arranged by size: 3221 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2584 2748 3256 4286 4883 5670 Size: 393 563 389 164 508 1030 597 787 Cuts at: 5670 5938 1239 Size: 268 1524 Fragments arranged by size: 1524 1030 787 597 563 508 393 389 268 164 EcoO109I rG'GnC_Cy Cuts at: 2652 2652 Size: 6223 EcoRI G'AATT_C Cuts at: 2970 3138 3390 3532 3910 2970 Size: 168 252 142 378 5283 Fragments arranged by size: 5283 378 252 168 142 EcoRV GAT'ATC Cuts at: 2865 2865 Size: 6223 FspI TGC'GCA Cuts at: 4729 4729 Size: 6223 HaeII r_GCGC'y Cuts at: 1678 5370 1678 Size: 3692 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 2601 2633 4864 Size: 32 135 32 689 32 308 32 2231 Cuts at: 4864 4896 4938 4970 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2231 689 308 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3296 3567 3813 669 Size: 988 281 1358 271 246 3079 Fragments arranged by size: 3079 1358 988 281 271 246 HindIII A'AGCT_T Cuts at: 748 2511 3435 748 Size: 1763 924 3536 Fragments arranged by size: 3536 1763 924 HpaI GTT'AAC Cuts at: 3813 3813 Size: 6223 KpnI G_GTAC'C Cuts at: 3548 3548 Size: 6223 MfeI C'AATT_G Cuts at: 3822 3822 Size: 6223 MluI A'CGCG_T Cuts at: 6099 6099 Size: 6223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2220 5337 5521 1305 Size: 915 3117 184 2007 Fragments arranged by size: 3117 2007 915 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 10 Size: 54 1425 343 384 4017 Fragments arranged by size: 4017 1425 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2163 2314 2358 2502 2979 3063 4181 Size: 648 997 151 44 144 477 84 1118 Cuts at: 4181 4540 4699 518 Size: 359 159 2042 Fragments arranged by size: 2042 1118 997 648 477 359 159 151 144 84 44 MspA1I CmG'CkG Cuts at: 1606 3639 4316 5464 5709 1606 Size: 2033 677 1148 245 2120 Fragments arranged by size: 2120 2033 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2287 513 Size: 552 1222 4449 Fragments arranged by size: 4449 1222 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6223 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6223 NotI GC'GGCC_GC Cuts at: 3670 3670 Size: 6223 NsiI A_TGCA'T Cuts at: 3228 3228 Size: 6223 NspI r_CATG'y Cuts at: 1132 1229 3581 5126 1132 Size: 97 2352 1545 2229 Fragments arranged by size: 2352 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6223 PciI A'CATG_T Cuts at: 5122 5122 Size: 6223 PflMI CCAn_nnn'nTGG Cuts at: 1108 2015 1108 Size: 907 5316 PfoI T'CCnGG_A Cuts at: 2050 2050 Size: 6223 PmeI GTTT'AAAC Cuts at: 3527 3527 Size: 6223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4292 4324 5805 5837 4292 Size: 32 1481 32 4678 Fragments arranged by size: 4678 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6223 PsiI TTA'TAA Cuts at: 3793 3793 Size: 6223 PstI C_TGCA'G Cuts at: 830 3575 830 Size: 2745 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6223 SalI G'TCGA_C Cuts at: 1936 3565 1936 Size: 1629 4594 SapI GCTCTTCn'nnn_ Cuts at: 2247 2247 Size: 6223 SbfI CC_TGCA'GG Cuts at: 3575 3575 Size: 6223 ScaI AGT'ACT Cuts at: 4471 6183 4471 Size: 1712 4511 SfcI C'TryA_G Cuts at: 826 1045 2182 2518 3571 4706 5387 5578 826 Size: 219 1137 336 1053 1135 681 191 1471 Fragments arranged by size: 1471 1137 1135 1053 681 336 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6223 SmaI CCC'GGG Cuts at: 1872 3550 1872 Size: 1678 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4327 5228 5490 820 Size: 197 926 2384 901 262 1553 Fragments arranged by size: 2384 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4014 493 Size: 3521 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6223 SphI G_CATG'C Cuts at: 1132 3581 1132 Size: 2449 3774 SspI AAT'ATT Cuts at: 5 52 4147 5 Size: 47 4095 2081 Fragments arranged by size: 4095 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6223 StyI C'CwwG_G Cuts at: 513 1065 2287 3662 513 Size: 552 1222 1375 3074 Fragments arranged by size: 3074 1375 1222 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2485 4250 4267 4420 4605 5050 325 Size: 1255 905 1765 17 153 185 445 1498 Fragments arranged by size: 1765 1498 1255 905 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2747 3500 4469 Size: 274 80 33 51 1443 770 753 969 Cuts at: 4469 6181 96 Size: 1712 138 Fragments arranged by size: 1712 1443 969 770 753 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2688 2720 6183 6215 2688 Size: 32 3463 32 2696 Fragments arranged by size: 3463 2696 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6223 XbaI T'CTAG_A Cuts at: 3559 3559 Size: 6223 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6223 XmaI C'CCGG_G Cuts at: 1870 3548 1870 Size: 1678 4545 XmnI GAAnn'nnTTC Cuts at: 2429 2933 3017 3101 3269 3437 4352 2429 Size: 504 84 84 168 168 915 4300 Fragments arranged by size: 4300 915 504 168 168 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4031 276 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI