(Circular) (Six-base) MAPSORT of: pFN21AB7554.seq Check: 7900 from: 1 to: 5515 pFN21AB7554 5515 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2858 1937 Size: 921 4594 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3578 4962 5230 1632 Size: 1946 1384 268 1917 Fragments arranged by size: 1946 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 4414 5391 1203 Size: 3211 977 1327 Fragments arranged by size: 3211 1327 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5515 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5515 AlwNI CAG_nnn'CTG Cuts at: 2646 3258 4830 2646 Size: 612 1572 3331 Fragments arranged by size: 3331 1572 612 ApaI G_GGCC'C Cuts at: 2130 2300 2130 Size: 170 5345 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2468 2824 3050 3202 5311 1493 Size: 975 356 226 152 2109 1697 Fragments arranged by size: 2109 1697 975 356 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2021 2498 2840 1870 Size: 73 78 477 342 4545 Fragments arranged by size: 4545 477 342 78 73 BamHI G'GATC_C Cuts at: 2845 3212 2845 Size: 367 5148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2047 2836 4291 618 Size: 325 217 627 249 11 789 1455 1842 Fragments arranged by size: 1842 1455 789 627 325 249 217 11 BanII G_rGCy'C Cuts at: 1391 2130 2220 2300 2387 2834 1391 Size: 739 90 80 87 447 4072 Fragments arranged by size: 4072 739 447 90 87 80 BbeI G_GCGC'C Cuts at: 2040 2040 Size: 5515 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2742 928 Size: 971 843 3701 Fragments arranged by size: 3701 971 843 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3706 3740 1903 Size: 34 50 34 1685 34 3678 Fragments arranged by size: 3678 1685 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 2650 2767 1548 Size: 1102 117 4296 Fragments arranged by size: 4296 1102 117 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2264 4126 136 Size: 107 122 71 631 605 592 1862 1525 Fragments arranged by size: 1862 1525 631 605 592 122 107 71 BglII A'GATC_T Cuts at: 5510 5510 Size: 5515 BlpI GC'TnA_GC Cuts at: 2531 2932 2531 Size: 401 5114 Bme1580I G_kGCm'C Cuts at: 1790 2130 2300 3576 4732 1790 Size: 340 170 1276 1156 2573 Fragments arranged by size: 2573 1276 1156 340 170 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5515 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2664 3246 4204 Size: 416 53 83 399 24 1247 582 958 Cuts at: 4204 442 Size: 1753 Fragments arranged by size: 1753 1247 958 582 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2401 2480 2832 4175 1239 Size: 1162 79 352 1343 2579 Fragments arranged by size: 2579 1343 1162 352 79 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3640 4505 4803 3640 Size: 865 298 4352 Fragments arranged by size: 4352 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4178 882 Size: 617 2679 2219 Fragments arranged by size: 2679 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3306 493 Size: 711 2102 2702 Fragments arranged by size: 2702 2102 711 BsaBI GATnn'nnATC Cuts at: 2766 5509 2766 Size: 2743 2772 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 2093 3322 3704 Size: 53 83 186 936 504 56 1229 382 Cuts at: 3704 4339 275 Size: 635 1451 Fragments arranged by size: 1451 1229 936 635 504 382 186 83 56 53 BsaWI w'CCGG_w Cuts at: 2199 3948 4346 4620 4767 5178 2199 Size: 1749 398 274 147 411 2536 Fragments arranged by size: 2536 1749 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5290 Fragments arranged by size: 5290 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2161 2596 1186 Size: 975 435 4105 Fragments arranged by size: 4105 975 435 BseYI C'CCAG_C Cuts at: 4718 5214 4718 Size: 496 5019 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4942 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 3726 4754 1540 Size: 275 186 964 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3576 3661 4732 3576 Size: 85 1071 4359 Fragments arranged by size: 4359 1071 85 BsmI GAATG_Cn' Cuts at: 3026 3119 3026 Size: 93 5422 BsmBI CGTCTCn'nnnn_ Cuts at: 2468 2468 Size: 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2220 2300 2387 2834 3576 3661 Size: 399 340 90 80 87 447 742 85 Cuts at: 3661 4732 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 340 90 87 85 80 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 3404 5134 3404 Size: 1730 3785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2870 844 Size: 2026 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3402 1394 Size: 429 1579 3507 Fragments arranged by size: 3507 1579 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4010 4184 65 Size: 1183 2762 174 1396 Fragments arranged by size: 2762 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2026 4159 4346 1700 Size: 110 216 2133 187 2869 Fragments arranged by size: 2869 2133 216 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5515 BssSI C'ACGA_G Cuts at: 2186 3575 4587 2186 Size: 1389 1012 3114 Fragments arranged by size: 3114 1389 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2344 2665 794 Size: 1550 321 3644 Fragments arranged by size: 3644 1550 321 BstBI TT'CG_AA Cuts at: 4319 4319 Size: 5515 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5515 BstXI CCAn_nnnn'nTGG Cuts at: 2458 2458 Size: 5515 BstYI r'GATC_y Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510 Size: 873 367 385 17 1452 86 12 346 Cuts at: 5510 1972 Size: 1977 Fragments arranged by size: 1977 1452 873 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2040 2122 2315 513 Size: 552 975 82 193 3713 Fragments arranged by size: 3713 975 552 193 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4776 Fragments arranged by size: 4776 683 56 BtsI GCAGTG_nn' Cuts at: 3031 3825 3845 3031 Size: 794 20 4701 Fragments arranged by size: 4701 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2819 2819 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4522 809 Size: 507 3206 1802 Fragments arranged by size: 3206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2265 2422 2514 Size: 54 1425 50 275 18 435 157 92 Cuts at: 2514 2962 3851 5480 8 Size: 448 889 1629 43 Fragments arranged by size: 1629 1425 889 448 435 275 157 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 1537 Size: 275 1150 4090 Fragments arranged by size: 4090 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3445 1353 Size: 2092 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2242 2339 2484 4102 4476 4622 Size: 1064 138 653 97 145 1618 374 146 Cuts at: 4622 387 Size: 1280 Fragments arranged by size: 1618 1280 1064 653 374 146 145 138 97 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2401 2480 2832 3578 4175 4962 5230 Size: 393 769 79 352 746 597 787 268 Cuts at: 5230 1239 Size: 1524 Fragments arranged by size: 1524 787 769 746 597 393 352 268 79 EcoO109I rG'GnC_Cy Cuts at: 2296 2297 2296 Size: 1 5514 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 2040 4662 1678 Size: 362 2622 2531 Fragments arranged by size: 2622 2531 362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2494 2526 2729 2761 4156 Size: 32 135 32 922 32 203 32 1395 Cuts at: 4156 4188 4230 4262 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1395 922 203 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2859 3105 669 Size: 988 281 921 246 3079 Fragments arranged by size: 3079 988 921 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5515 HpaI GTT'AAC Cuts at: 3105 3105 Size: 5515 KasI G'GCGC_C Cuts at: 2036 2036 Size: 5515 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 3114 3114 Size: 5515 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2463 4629 4813 1305 Size: 1158 2166 184 2007 Fragments arranged by size: 2166 2007 1158 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2516 10 Size: 54 1425 343 684 3009 Fragments arranged by size: 3009 1425 684 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3473 3832 3991 518 Size: 648 2307 359 159 2042 Fragments arranged by size: 2307 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2053 2071 2124 2270 2317 2931 3608 4756 Size: 447 18 53 146 47 614 677 1148 Cuts at: 4756 5001 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 614 447 245 146 53 47 18 NaeI GCC'GGC Cuts at: 2028 2028 Size: 5515 NarI GG'CG_CC Cuts at: 2037 2037 Size: 5515 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4963 NdeI CA'TA_TG Cuts at: 387 387 Size: 5515 NgoMIV G'CCGG_C Cuts at: 2026 2026 Size: 5515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2873 4418 1132 Size: 97 1644 1545 2229 Fragments arranged by size: 2229 1644 1545 97 PasI CC'CwG_GG Cuts at: 1348 2362 1348 Size: 1014 4501 PciI A'CATG_T Cuts at: 4414 4414 Size: 5515 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5515 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 5097 5129 3584 Size: 32 1481 32 3970 Fragments arranged by size: 3970 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PspOMI G'GGCC_C Cuts at: 2126 2296 2126 Size: 170 5345 PstI C_TGCA'G Cuts at: 830 2529 2867 830 Size: 1699 338 3478 Fragments arranged by size: 3478 1699 338 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 SacII CC_GC'GG Cuts at: 2125 2318 2125 Size: 193 5322 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 3763 5475 3763 Size: 1712 3803 SexAI A'CCwGG_T Cuts at: 2552 2552 Size: 5515 SfcI C'TryA_G Cuts at: 826 1045 2525 2863 3998 4679 4870 826 Size: 219 1480 338 1135 681 191 1471 Fragments arranged by size: 1480 1471 1135 681 338 219 191 SfoI GGC'GCC Cuts at: 2038 2038 Size: 5515 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2023 2842 1872 Size: 151 819 4545 Fragments arranged by size: 4545 819 151 SmlI C'TyrA_G Cuts at: 820 1017 1943 3619 4520 4782 820 Size: 197 926 1676 901 262 1553 Fragments arranged by size: 1676 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2873 1132 Size: 1741 3774 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 2401 1517 Size: 884 4631 StyI C'CwwG_G Cuts at: 513 1065 2954 513 Size: 552 1889 3074 Fragments arranged by size: 3074 1889 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3542 3559 3712 3897 4342 325 Size: 1255 1962 17 153 185 445 1498 Fragments arranged by size: 1962 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3761 5473 96 Size: 274 80 33 51 1443 1784 1712 138 Fragments arranged by size: 1784 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5475 5507 5475 Size: 32 5483 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2021 2840 1870 Size: 151 819 4545 Fragments arranged by size: 4545 819 151 XmnI GAAnn'nnTTC Cuts at: 2468 3644 2468 Size: 1176 4339 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SrfI SwaI XcmI