(Circular) (Six-base) MAPSORT of: pFN21AE0778.seq Check: 488 from: 1 to: 6247 pFN21AE0778 6247 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4057 278 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 3590 1937 Size: 1413 240 4594 Fragments arranged by size: 4594 1413 240 Acc65I G'GTAC_C Cuts at: 3568 3568 Size: 6247 AclI AA'CG_TT Cuts at: 1563 4374 4747 1563 Size: 2811 373 3063 Fragments arranged by size: 3063 2811 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2901 3288 3309 4310 5694 5962 1632 Size: 1269 387 21 1001 1384 268 1917 Fragments arranged by size: 1917 1384 1269 1001 387 268 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6050 AflIII A'CryG_T Cuts at: 1203 2852 2946 5146 6123 1203 Size: 1649 94 2200 977 1327 Fragments arranged by size: 2200 1649 1327 977 94 AgeI A'CCGG_T Cuts at: 5078 5078 Size: 6247 AhdI GACnn_n'nnGTC Cuts at: 4976 4976 Size: 6247 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3390 1184 Size: 2206 4041 AlwNI CAG_nnn'CTG Cuts at: 3990 5562 3990 Size: 1572 4675 ApaLI G'TGCA_C Cuts at: 4304 5460 4304 Size: 1156 5091 ApoI r'AATT_y Cuts at: 1493 2610 3046 3556 3782 3934 6043 1493 Size: 1117 436 510 226 152 2109 1697 Fragments arranged by size: 2109 1697 1117 510 436 226 152 AseI AT'TA_AT Cuts at: 160 1032 4801 160 Size: 872 3769 1606 Fragments arranged by size: 3769 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 AvaI C'yCGr_G Cuts at: 1870 1943 3572 1870 Size: 73 1629 4545 Fragments arranged by size: 4545 1629 73 BamHI G'GATC_C Cuts at: 3577 3944 3577 Size: 367 5880 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3518 3568 5023 618 Size: 325 217 627 1731 50 1455 1842 Fragments arranged by size: 1842 1731 1455 627 325 217 50 BanII G_rGCy'C Cuts at: 1391 3566 1391 Size: 2175 4072 BbeI G_GCGC'C Cuts at: 3522 3522 Size: 6247 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2917 2951 4438 4472 1903 Size: 34 980 34 1487 34 3678 Fragments arranged by size: 3678 1487 980 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4139 5349 4139 Size: 1210 5037 BclI T'GATC_A Cuts at: 1548 1548 Size: 6247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4858 136 Size: 107 122 71 631 605 3186 1525 Fragments arranged by size: 3186 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2716 6242 2716 Size: 3526 2721 BlpI GC'TnA_GC Cuts at: 2806 3664 2806 Size: 858 5389 Bme1580I G_kGCm'C Cuts at: 1790 2896 4308 5464 1790 Size: 1106 1412 1156 2573 Fragments arranged by size: 2573 1412 1156 1106 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6247 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3978 4936 442 Size: 416 53 83 399 24 2561 958 1753 Fragments arranged by size: 2561 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 2195 2200 4907 1239 Size: 855 101 5 2707 2579 Fragments arranged by size: 2707 2579 855 101 5 Bpu10I CC'TnA_GC Cuts at: 3120 3120 Size: 6247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2218 4372 5237 5535 2218 Size: 2154 865 298 2930 Fragments arranged by size: 2930 2154 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3166 4910 882 Size: 617 1667 1744 2219 Fragments arranged by size: 2219 1744 1667 617 BsaAI yAC'GTr Cuts at: 493 1204 2957 4038 493 Size: 711 1753 1081 2702 Fragments arranged by size: 2702 1753 1081 711 BsaBI GATnn'nnATC Cuts at: 6241 6241 Size: 6247 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2934 3519 4054 4436 Size: 53 83 186 936 1401 585 535 382 Cuts at: 4436 5071 275 Size: 635 1451 Fragments arranged by size: 1451 1401 936 635 585 535 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4680 5078 5352 5499 5910 4680 Size: 398 274 147 411 5017 Fragments arranged by size: 5017 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6022 Fragments arranged by size: 6022 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3350 1186 Size: 2164 4083 BseYI C'CCAG_C Cuts at: 5450 5946 5450 Size: 496 5751 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2175 3424 1163 Size: 573 439 1249 3986 Fragments arranged by size: 3986 1249 573 439 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3697 4458 5486 1540 Size: 275 186 1696 761 1028 2301 Fragments arranged by size: 2301 1696 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4308 4393 5464 4308 Size: 85 1071 5091 Fragments arranged by size: 5091 1071 85 BsmI GAATG_Cn' Cuts at: 3758 3851 3758 Size: 93 6154 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2896 3566 4308 4393 5464 1391 Size: 399 1106 670 742 85 1071 2174 Fragments arranged by size: 2174 1106 1071 742 670 399 85 BspEI T'CCGG_A Cuts at: 5910 5910 Size: 6247 BspHI T'CATG_A Cuts at: 4136 5866 4136 Size: 1730 4517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3602 844 Size: 2758 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4134 1394 Size: 429 2311 3507 Fragments arranged by size: 3507 2311 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4742 4916 65 Size: 1183 3494 174 1396 Fragments arranged by size: 3494 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4891 5078 1700 Size: 110 3081 187 2869 Fragments arranged by size: 3081 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2707 2943 96 Size: 2611 236 3400 Fragments arranged by size: 3400 2611 236 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6247 BssSI C'ACGA_G Cuts at: 4307 5319 4307 Size: 1012 5235 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6247 BstBI TT'CG_AA Cuts at: 5051 5051 Size: 6247 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6247 BstYI r'GATC_y Cuts at: 1972 2716 3097 3577 3944 4329 4346 5798 5884 Size: 744 381 480 367 385 17 1452 86 Cuts at: 5884 5896 6242 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 744 480 385 381 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4756 Fragments arranged by size: 4756 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5508 Fragments arranged by size: 5508 683 56 BtsI GCAGTG_nn' Cuts at: 2918 3763 4557 4577 2918 Size: 845 794 20 4588 Fragments arranged by size: 4588 845 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6247 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2955 2990 529 Size: 35 2391 35 3786 Fragments arranged by size: 3786 2391 35 35 DraI TTT'AAA Cuts at: 3551 3551 Size: 6247 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5254 809 Size: 507 3938 1802 Fragments arranged by size: 3938 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212 Size: 54 1425 50 275 18 1864 889 1629 Cuts at: 6212 8 Size: 43 Fragments arranged by size: 1864 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3694 1537 Size: 275 1882 4090 Fragments arranged by size: 4090 1882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2982 3240 3327 4177 1353 Size: 1629 258 87 850 3423 Fragments arranged by size: 3423 1629 850 258 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4834 5208 5354 387 Size: 1064 138 3245 374 146 1280 Fragments arranged by size: 3245 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2195 2200 2901 3288 3309 4310 Size: 393 462 101 5 701 387 21 1001 Cuts at: 4310 4907 5694 5962 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1001 787 701 597 462 393 387 268 101 21 5 EcoRI G'AATT_C Cuts at: 3046 3556 3934 3046 Size: 510 378 5359 Fragments arranged by size: 5359 510 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2093 3011 3409 2093 Size: 918 398 4931 Fragments arranged by size: 4931 918 398 FspI TGC'GCA Cuts at: 4753 4753 Size: 6247 HaeII r_GCGC'y Cuts at: 1678 3522 5394 1678 Size: 1844 1872 2531 Fragments arranged by size: 2531 1872 1844 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2039 2047 2071 2198 Size: 32 135 32 443 24 8 24 127 Cuts at: 2198 2216 2230 2248 4888 4920 4962 4994 1373 Size: 18 14 18 2640 32 42 32 2626 Fragments arranged by size: 2640 2626 443 135 127 42 32 32 32 32 24 24 18 18 14 8 HincII GTy'rAC Cuts at: 669 1657 1938 3147 3591 3837 669 Size: 988 281 1209 444 246 3079 Fragments arranged by size: 3079 1209 988 444 281 246 HindIII A'AGCT_T Cuts at: 748 2993 748 Size: 2245 4002 HpaI GTT'AAC Cuts at: 3147 3837 3147 Size: 690 5557 KasI G'GCGC_C Cuts at: 3518 3518 Size: 6247 KpnI G_GTAC'C Cuts at: 3572 3572 Size: 6247 MfeI C'AATT_G Cuts at: 3846 3846 Size: 6247 MluI A'CGCG_T Cuts at: 6123 6123 Size: 6247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5361 5545 1305 Size: 4056 184 2007 Fragments arranged by size: 4056 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4425 Fragments arranged by size: 4425 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2697 2789 4205 4564 4723 518 Size: 648 1531 92 1416 359 159 2042 Fragments arranged by size: 2042 1531 1416 648 359 159 92 MspA1I CmG'CkG Cuts at: 1606 3440 3663 4340 5488 5733 1606 Size: 1834 223 677 1148 245 2120 Fragments arranged by size: 2120 1834 1148 677 245 223 NarI GG'CG_CC Cuts at: 3519 3519 Size: 6247 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4756 Fragments arranged by size: 4756 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6247 NotI GC'GGCC_GC Cuts at: 3694 3694 Size: 6247 NspI r_CATG'y Cuts at: 1132 1229 2856 2950 3605 5150 1132 Size: 97 1627 94 655 1545 2229 Fragments arranged by size: 2229 1627 1545 655 97 94 PasI CC'CwG_GG Cuts at: 1348 3294 1348 Size: 1946 4301 PciI A'CATG_T Cuts at: 2852 2946 5146 2852 Size: 94 2200 3953 Fragments arranged by size: 3953 2200 94 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6247 PfoI T'CCnGG_A Cuts at: 2216 2216 Size: 6247 PmeI GTTT'AAAC Cuts at: 3551 3551 Size: 6247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4316 4348 5829 5861 4316 Size: 32 1481 32 4702 Fragments arranged by size: 4702 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6247 PsiI TTA'TAA Cuts at: 3817 3817 Size: 6247 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2514 2546 2514 Size: 32 6215 PstI C_TGCA'G Cuts at: 830 2847 3599 830 Size: 2017 752 3478 Fragments arranged by size: 3478 2017 752 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6247 PvuII CAG'CTG Cuts at: 3440 3440 Size: 6247 SalI G'TCGA_C Cuts at: 1936 3589 1936 Size: 1653 4594 SbfI CC_TGCA'GG Cuts at: 3599 3599 Size: 6247 ScaI AGT'ACT Cuts at: 4495 6207 4495 Size: 1712 4535 SfcI C'TryA_G Cuts at: 826 1045 2679 2843 2902 3595 4730 5411 5602 Size: 219 1634 164 59 693 1135 681 191 Cuts at: 5602 826 Size: 1471 Fragments arranged by size: 1634 1471 1135 693 681 219 191 164 59 SfoI GGC'GCC Cuts at: 3520 3520 Size: 6247 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6247 SmaI CCC'GGG Cuts at: 1872 3574 1872 Size: 1702 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2233 4351 5252 5514 820 Size: 197 926 290 2118 901 262 1553 Fragments arranged by size: 2118 1553 926 901 290 262 197 SnaBI TAC'GTA Cuts at: 493 4038 493 Size: 3545 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6247 SphI G_CATG'C Cuts at: 1132 3605 1132 Size: 2473 3774 SspI AAT'ATT Cuts at: 5 52 2367 4171 5 Size: 47 2315 1804 2081 Fragments arranged by size: 2315 2081 1804 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6247 StyI C'CwwG_G Cuts at: 513 1065 2004 3484 3686 513 Size: 552 939 1480 202 3074 Fragments arranged by size: 3074 1480 939 552 202 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4274 4291 4444 4629 5074 325 Size: 1255 2694 17 153 185 445 1498 Fragments arranged by size: 2694 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2074 2707 2943 Size: 274 80 33 51 1443 97 633 236 Cuts at: 2943 4493 6205 96 Size: 1550 1712 138 Fragments arranged by size: 1712 1550 1443 633 274 236 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6207 6239 6207 Size: 32 6215 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6247 XbaI T'CTAG_A Cuts at: 3583 3583 Size: 6247 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6247 XmaI C'CCGG_G Cuts at: 1870 3572 1870 Size: 1702 4545 XmnI GAAnn'nnTTC Cuts at: 4376 4376 Size: 6247 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4055 276 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI