Restriction map for
pFN21AE0741
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MluI MluNI MroI MscI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI TspRI Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 518 GGCGTC G[GA]^CG[CT]C
AfaI 768 GTAC GT^AC
AlwI 477 GGATC GGATC(4/5)
AspHI 193 GAGCTC G[AT]GC[AT]^C
AvaI 104 CCCGGG C^[CT]CG[GA]G
AvaII 691 GGTCC G^G[AT]CC
BbsI 127 GTCTTC GAAGAC(2/6)
BbvCI 615 GCTGAGG CCTCAGC(-5/-2)
BglII 239 AGATCT A^GATCT
BpmI 180 CTCCAG CTGGAG(16/14)
BpuAI 127 GTCTTC GAAGAC(2/6)
BsaHI 518 GGCGTC G[GA]^CG[CT]C
BsiHKAI 193 GAGCTC G[AT]GC[AT]^C
BsmFI 102 GTCCC GGGAC(10/14)
BsoBI 104 CCCGGG C^[CT]CG[GA]G
Bsp1407I 767 TGTACA T^GTACA
BsrGI 767 TGTACA T^GTACA
BstF5I 186 CATCC GGATG(2/0)
BstNI 189 CCAGG CC^[AT]GG
BstYI 239 AGATCT [GA]^GATC[CT]
DraI 255 TTTAAA TTT^AAA
DraII 690 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 889 GACGAATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 935 GAAGAG CTCTTC(1/4)
EcoO109I 690 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 776 GAATTC G^AATTC
EcoRII 189 CCAGG ^CC[AT]GG
EcoT14I 38 CCAAGG C^C[AT][AT]GG
FokI 186 CATCC GGATG(9/13)
Hin1I 518 GGCGTC G[GA]^CG[CT]C
Ksp632I 935 GAAGAG CTCTTC(1/4)
MaeIII 521 GTCAC ^GT[ACGT]AC
MfeI 737 CAATTG C^AATTG
MflI 239 AGATCT [GA]^GATC[CT]
MspA1I 139 CAGCTG C[AC]G^C[GT]G
MunI 737 CAATTG C^AATTG
MvaI 189 CCAGG CC^[AT]GG
PpuMI 690 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 139 CAGCTG CAG^CTG
RsaI 768 GTAC GT^AC
SacI 193 GAGCTC GAGCT^C
SmaI 104 CCCGGG CCC^GGG
SmlI 760 CTTGAG C^T[CT][GA]AG
SspBI 767 TGTACA T^GTACA
SspI 505 AATATT AAT^ATT
StyI 38 CCAAGG C^C[AT][AT]GG
Tsp45I 521 GTCAC ^GT[GC]AC
XcmI 158 CCACGGGCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 239 AGATCT [GA]^GATC[CT]
XmaCI 104 CCCGGG C^CCGGG
XmaI 104 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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BanII 107 GGGCTC G[GA]GC[CT]^C
193 GAGCTC
BbvI 138 GCAGC GCAGC(8/12)
12 GCTGC
BfaI 301 CTAG C^TAG
307 CTAG
BmyI 107 GGGCTC G[AGT]GC[ACT]^C
193 GAGCTC
BsaI 43 GAGACC GGTCTC(1/5)
382 GGTCTC
Bsp1286I 107 GGGCTC G[AGT]GC[ACT]^C
193 GAGCTC
Cfr13I 162 GGGCC G^G[ACGT]CC
691 GGTCC
DsaI 158 CCACGG C^C[GA][CT]GG
701 CCGTGG
HaeIII 30 GGCC GG^CC
163 GGCC
HapII 50 CCGG C^CGG
105 CCGG
HgaI 100 GCGTC GACGC(5/10)
519 GCGTC
HpaII 50 CCGG C^CGG
105 CCGG
HphI 922 GGTGA GGTGA(8/7)
386 TCACC
MaeI 301 CTAG C^TAG
307 CTAG
MspI 50 CCGG C^CGG
105 CCGG
NlaIV 89 GGAGCC GG[ACGT]^[ACGT]CC
108 GGCTCC
Sau96I 162 GGGCC G^G[ACGT]CC
691 GGTCC
SfaNI 925 GATGC GCATC(5/9)
185 GCATC
TaqI 341 TCGA T^CGA
780 TCGA
TseI 12 GCTGC G^C[AT]GC
138 GCAGC
TthHB8I 341 TCGA T^CGA
780 TCGA
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
BcnI BsaJI BsiYI BslI BsmAI
BssKI BstUI Cac8I CfoI DdeI
DpnII FauI Fnu4HI HhaI HinP1I
HinfI ItaI MboI MboII MnlI
MseI MslI MvnI MwoI NciI
NdeII NlaIII PleI Sau3AI ScrFI
TfiI Tru9I Tsp509I