Restriction map for
pFN21AE0741
(Circular) (Six-base) MAPSORT of: pFN21AE0741.seq Check: 9143 from: 1 to: 5650
pFN21AE0741 5650 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 3713 5097 5365 1632
Size: 401 1680 1384 268 1917
Fragments arranged by size:
1917 1680 1384 401 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 2157 1166
Size: 991 4659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2175 1184
Size: 991 4659
AlwNI CAG_nnn'CTG
Cuts at: 3393 4965 3393
Size: 1572 4078
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2781 2846 2897 2959 3185 3337 5446 1493
Size: 1288 65 51 62 226 152 2109 1697
Fragments arranged by size:
2109 1697 1288 226 152 65 62 51
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 2975 1870
Size: 73 166 866 4545
Fragments arranged by size:
4545 866 166 73
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 4426 618
Size: 325 217 627 1184 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2116 2202 2969 1391
Size: 725 86 767 4072
Fragments arranged by size:
4072 767 725 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2125 928
Size: 971 226 4453
Fragments arranged by size:
4453 971 226
BbvCI CC'TCA_GC
Cuts at: 2621 2621
Size: 5650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3841 3875 1903
Size: 34 50 34 1820 34 3678
Fragments arranged by size:
3678 1820 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2244 5645 2244
Size: 3401 2249
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 3711 4867 1790
Size: 1921 1156 2573
Fragments arranged by size:
2573 1921 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2170 4310 1239
Size: 931 2140 2579
Fragments arranged by size:
2579 2140 931
Bpu10I CC'TnA_GC
Cuts at: 2621 2621
Size: 5650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2786 3775 4640 4938 2786
Size: 989 865 298 3498
Fragments arranged by size:
3498 989 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 2393 4313 882
Size: 617 543 351 1920 2219
Fragments arranged by size:
2219 1920 617 543 351
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2524 3457 3839 4474
Size: 53 83 186 936 991 933 382 635
Cuts at: 4474 275
Size: 1451
Fragments arranged by size:
1451 991 936 933 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5650
BseYI C'CCAG_C
Cuts at: 4853 5349 4853
Size: 496 5154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5077
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 3711 3796 4867 2202
Size: 1509 85 1071 2985
Fragments arranged by size:
2985 1509 1071 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2202 2969 3711 3796 4867 1391
Size: 399 326 86 767 742 85 1071 2174
Fragments arranged by size:
2174 1071 767 742 399 326 86 85
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3005 844
Size: 2161 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4145 4319 65
Size: 1183 2897 174 1396
Fragments arranged by size:
2897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 2772 96
Size: 2676 2974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2244 2980 3347 3732 3749 5201 5287 5299
Size: 272 736 367 385 17 1452 86 12
Cuts at: 5299 5645 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 736 385 367 346 272 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2163 2706 513
Size: 552 1098 543 3457
Fragments arranged by size:
3457 1098 552 543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3960 3980 3166
Size: 794 20 4836
Fragments arranged by size:
4836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2794 2829 529
Size: 35 2230 35 3350
Fragments arranged by size:
3350 2230 35 35
DraI TTT'AAA
Cuts at: 2262 2954 2262
Size: 692 4958
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2900 4657 809
Size: 507 1584 1757 1802
Fragments arranged by size:
1802 1757 1584 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3097 3986 5615
Size: 54 1425 50 275 18 1267 889 1629
Cuts at: 5615 8
Size: 43
Fragments arranged by size:
1629 1425 1267 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2935 3580 1353
Size: 1582 645 3423
Fragments arranged by size:
3423 1582 645
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2200 2200
Size: 5650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2170 3713 4310 5097 5365 1239
Size: 393 401 137 1543 597 787 268 1524
Fragments arranged by size:
1543 1524 787 597 401 393 268 137
EcoO109I rG'GnC_Cy
Cuts at: 2696 2696
Size: 5650
EcoRI G'AATT_C
Cuts at: 2781 2959 3337 2781
Size: 178 378 5094
Fragments arranged by size:
5094 378 178
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 4291 4323 4365
Size: 32 135 32 1037 32 1650 32 42
Cuts at: 4365 4397 1373
Size: 32 2626
Fragments arranged by size:
2626 1650 1037 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 2742 3249 2742
Size: 507 5143
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 4764 4948 1305
Size: 915 2544 184 2007
Fragments arranged by size:
2544 2007 915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3828
Fragments arranged by size:
3828 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 2532 2807 3608 3967 4126 518
Size: 648 991 375 275 801 359 159 2042
Fragments arranged by size:
2042 991 801 648 375 359 275 159
MspA1I CmG'CkG
Cuts at: 1606 2146 3066 3743 4891 5136 1606
Size: 540 920 677 1148 245 2120
Fragments arranged by size:
2120 1148 920 677 540 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5098
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 3008 4553 1132
Size: 97 1779 1545 2229
Fragments arranged by size:
2229 1779 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5650
PfoI T'CCnGG_A
Cuts at: 2193 2193
Size: 5650
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2696 2696
Size: 5650
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 2426 3220 2426
Size: 794 4856
PstI C_TGCA'G
Cuts at: 830 3002 830
Size: 2172 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2146 2146
Size: 5650
SacI G_AGCT'C
Cuts at: 2202 2202
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2998 4133 4814 5005 826
Size: 219 1953 1135 681 191 1471
Fragments arranged by size:
1953 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2111 2977 1872
Size: 239 866 4545
Fragments arranged by size:
4545 866 239
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2765 3754 4655 4917 820
Size: 197 926 822 989 901 262 1553
Fragments arranged by size:
1553 989 926 901 822 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 2512 3574 5
Size: 47 2460 1062 2081
Fragments arranged by size:
2460 2081 1062 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 2043 3089 513
Size: 552 978 1046 3074
Fragments arranged by size:
3074 1046 978 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2508 3677 3694 3847 4032 4477 325
Size: 1255 928 1169 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1169 928 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2772 3896 5608
Size: 274 80 33 51 1443 795 1124 1712
Cuts at: 5608 96
Size: 138
Fragments arranged by size:
1712 1443 1124 795 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2170 2170
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2109 2975 1870
Size: 239 866 4545
Fragments arranged by size:
4545 866 239
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI