Restriction map for
pFN21AE0741
(Linear) (Six-base) MAPSORT of: KIEE0741.seq Check: 2520 from: 1 to: 945
KIEE0741.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 945
Size: 28 917
AleI CACnn'nnGTG
Cuts at: 0 152 945
Size: 152 793
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 170 945
Size: 170 775
ApoI r'AATT_y
Cuts at: 0 776 841 892 945
Size: 776 65 51 53
Fragments arranged by size:
776 65 53 51
AvaI C'yCGr_G
Cuts at: 0 104 945
Size: 104 841
BanII G_rGCy'C
Cuts at: 0 111 197 945
Size: 111 86 748
Fragments arranged by size:
748 111 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 120 945
Size: 120 825
BbvCI CC'TCA_GC
Cuts at: 0 616 945
Size: 616 329
BglII A'GATC_T
Cuts at: 0 239 945
Size: 239 706
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 195 945
Size: 195 750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 945
Size: 165 780
Bpu10I CC'TnA_GC
Cuts at: 0 616 945
Size: 616 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 945
Size: 781 164
BsaI GGTCTCn'nnnn_
Cuts at: 0 37 388 945
Size: 37 351 557
Fragments arranged by size:
557 351 37
BsaHI Gr'CG_yC
Cuts at: 0 519 945
Size: 519 426
BsiHKAI G_wGCw'C
Cuts at: 0 197 945
Size: 197 748
Bsp1286I G_dGCh'C
Cuts at: 0 111 197 945
Size: 111 86 748
Fragments arranged by size:
748 111 86
BsrGI T'GTAC_A
Cuts at: 0 767 945
Size: 767 178
BstYI r'GATC_y
Cuts at: 0 239 945
Size: 239 706
BtgI C'CryG_G
Cuts at: 0 158 701 945
Size: 158 543 244
Fragments arranged by size:
543 244 158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 789 824 945
Size: 789 35 121
Fragments arranged by size:
789 121 35
DraI TTT'AAA
Cuts at: 0 257 945
Size: 257 688
DrdI GACnn_nn'nnGTC
Cuts at: 0 895 945
Size: 895 50
EarI CTCTTCn'nnn_
Cuts at: 0 930 945
Size: 930 15
EcoICRI GAG'CTC
Cuts at: 0 195 945
Size: 195 750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 165 945
Size: 28 137 780
Fragments arranged by size:
780 137 28
EcoO109I rG'GnC_Cy
Cuts at: 0 691 945
Size: 691 254
EcoRI G'AATT_C
Cuts at: 0 776 945
Size: 776 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 945
Size: 604 32 309
Fragments arranged by size:
604 309 32
MfeI C'AATT_G
Cuts at: 0 737 945
Size: 737 208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 945
Size: 215 730
MslI CAynn'nnrTG
Cuts at: 0 152 527 802 945
Size: 152 375 275 143
Fragments arranged by size:
375 275 152 143
MspA1I CmG'CkG
Cuts at: 0 141 945
Size: 141 804
PfoI T'CCnGG_A
Cuts at: 0 188 945
Size: 188 757
PpuMI rG'GwC_Cy
Cuts at: 0 691 945
Size: 691 254
PsiI TTA'TAA
Cuts at: 0 421 945
Size: 421 524
PvuII CAG'CTG
Cuts at: 0 141 945
Size: 141 804
SacI G_AGCT'C
Cuts at: 0 197 945
Size: 197 748
SmaI CCC'GGG
Cuts at: 0 106 945
Size: 106 839
SmlI C'TyrA_G
Cuts at: 0 760 945
Size: 760 185
SspI AAT'ATT
Cuts at: 0 507 945
Size: 507 438
StyI C'CwwG_G
Cuts at: 0 38 945
Size: 38 907
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 503 945
Size: 503 442
TatI w'GTAC_w
Cuts at: 0 767 945
Size: 767 178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 945
Size: 165 780
XmaI C'CCGG_G
Cuts at: 0 104 945
Size: 104 841
Enzymes that do cut:
AcuI AleI AlfI ApoI AvaI BanII BbsI BbvCI
BglII BplI BpmI Bpu10I BpuEI BsaI BsaHI BsiHKAI
Bsp1286I BsrGI BstYI BtgI CspCI DraI DrdI EarI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I MfeI MmeI MslI
MspA1I PfoI PpuMI PsiI PvuII SacI SmaI SmlI
SspI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI DraIII EaeI EagI EciI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI