(Circular) (Six-base) MAPSORT of: pFN21AE0719.seq Check: 3077 from: 1 to: 5620 pFN21AE0719 5620 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2963 1937 Size: 1026 4594 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 2198 2285 2408 3683 5067 5335 1632 Size: 488 78 87 123 1275 1384 268 1917 Fragments arranged by size: 1917 1384 1275 488 268 123 87 78 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 2552 1166 Size: 1386 4234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2804 1184 Size: 1620 4000 AlwNI CAG_nnn'CTG Cuts at: 2263 2338 2401 3363 4935 2263 Size: 75 63 962 1572 2948 Fragments arranged by size: 2948 1572 962 75 63 ApaLI G'TGCA_C Cuts at: 2213 3677 4833 2213 Size: 1464 1156 3000 Fragments arranged by size: 3000 1464 1156 ApoI r'AATT_y Cuts at: 1493 2929 3155 3307 5416 1493 Size: 1436 226 152 2109 1697 Fragments arranged by size: 2109 1697 1436 226 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2516 2687 2945 1870 Size: 73 573 171 258 4545 Fragments arranged by size: 4545 573 258 171 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2489 2522 2489 Size: 33 5587 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2420 2464 2941 4396 Size: 325 217 627 255 378 44 477 1455 Cuts at: 4396 618 Size: 1842 Fragments arranged by size: 1842 1455 627 477 378 325 255 217 44 BanII G_rGCy'C Cuts at: 1391 2301 2877 2939 1391 Size: 910 576 62 4072 Fragments arranged by size: 4072 910 576 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2682 928 Size: 971 783 3866 Fragments arranged by size: 3866 971 783 BbvCI CC'TCA_GC Cuts at: 2252 2252 Size: 5620 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3811 3845 1903 Size: 34 1874 34 3678 Fragments arranged by size: 3678 1874 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 1548 Size: 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4231 136 Size: 107 122 71 631 605 2559 1525 Fragments arranged by size: 2559 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2157 5615 2157 Size: 3458 2162 BlpI GC'TnA_GC Cuts at: 3037 3037 Size: 5620 Bme1580I G_kGCm'C Cuts at: 1790 2045 2217 2469 2763 3681 4837 1790 Size: 255 172 252 294 918 1156 2573 Fragments arranged by size: 2573 1156 918 294 255 252 172 BmgBI CAC'GTC Cuts at: 1318 2209 1318 Size: 891 4729 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3351 4309 442 Size: 416 53 83 399 24 1934 958 1753 Fragments arranged by size: 1934 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2584 2789 4280 1239 Size: 1345 205 1491 2579 Fragments arranged by size: 2579 1491 1345 205 Bpu10I CC'TnA_GC Cuts at: 2252 2871 2252 Size: 619 5001 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2153 3745 4610 4908 2153 Size: 1592 865 298 2865 Fragments arranged by size: 2865 1592 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2376 4283 882 Size: 617 877 1907 2219 Fragments arranged by size: 2219 1907 877 617 BsaAI yAC'GTr Cuts at: 493 1204 3411 493 Size: 711 2207 2702 Fragments arranged by size: 2702 2207 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4053 4451 4725 4872 5283 4053 Size: 398 274 147 411 4390 Fragments arranged by size: 4390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 2557 2587 1178 Size: 30 165 30 861 30 263 30 4211 Fragments arranged by size: 4211 861 263 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5620 BseYI C'CCAG_C Cuts at: 2659 2669 2697 2816 2881 4823 5319 2659 Size: 10 28 119 65 1942 496 2960 Fragments arranged by size: 2960 1942 496 119 65 28 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2291 1163 Size: 573 555 4492 Fragments arranged by size: 4492 573 555 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3070 3831 4859 1540 Size: 275 186 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 2301 3681 3766 4837 2217 Size: 84 1380 85 1071 3000 Fragments arranged by size: 3000 1380 1071 85 84 BsmI GAATG_Cn' Cuts at: 3131 3224 3131 Size: 93 5527 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2217 2301 2469 2763 2877 2939 Size: 399 255 172 84 168 294 114 62 Cuts at: 2939 3681 3766 4837 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 294 255 172 168 114 85 84 62 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 3509 5239 3509 Size: 1730 3890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2409 2796 2975 844 Size: 1565 387 179 3489 Fragments arranged by size: 3489 1565 387 179 BsrBI CCG'CTC Cuts at: 1394 1823 3507 1394 Size: 429 1684 3507 Fragments arranged by size: 3507 1684 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4115 4289 65 Size: 1183 2867 174 1396 Fragments arranged by size: 2867 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 2058 96 Size: 1962 3658 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 2574 2803 794 Size: 1780 229 3611 Fragments arranged by size: 3611 1780 229 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 2692 1184 Size: 1508 4112 BstXI CCAn_nnnn'nTGG Cuts at: 2431 2554 2431 Size: 123 5497 BstYI r'GATC_y Cuts at: 1972 2157 2950 3317 3702 3719 5171 5257 5269 Size: 185 793 367 385 17 1452 86 12 Cuts at: 5269 5615 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 793 385 367 346 185 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4129 Fragments arranged by size: 4129 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4881 Fragments arranged by size: 4881 683 56 BtsI GCAGTG_nn' Cuts at: 2142 3136 3930 3950 2142 Size: 994 794 20 3812 Fragments arranged by size: 3812 994 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2924 2924 Size: 5620 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3067 3956 5585 Size: 54 1425 50 275 18 1237 889 1629 Cuts at: 5585 8 Size: 43 Fragments arranged by size: 1629 1425 1237 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3067 1537 Size: 275 1255 4090 Fragments arranged by size: 4090 1255 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2333 2745 3550 1353 Size: 980 412 805 3423 Fragments arranged by size: 3423 980 805 412 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2903 4207 4581 4727 387 Size: 1064 138 1314 1304 374 146 1280 Fragments arranged by size: 1314 1304 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2299 2299 Size: 5620 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2198 2285 2408 2584 2789 3683 Size: 393 488 78 87 123 176 205 894 Cuts at: 3683 4280 5067 5335 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 894 787 597 488 393 268 205 176 123 87 78 EcoNI CCTnn'n_nnAGG Cuts at: 2255 2255 Size: 5620 EcoO109I rG'GnC_Cy Cuts at: 2134 2260 2785 2134 Size: 126 525 4969 Fragments arranged by size: 4969 525 126 EcoRI G'AATT_C Cuts at: 2929 3307 2929 Size: 378 5242 EcoRV GAT'ATC Cuts at: 2560 2560 Size: 5620 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2737 2737 Size: 5620 FspI TGC'GCA Cuts at: 2474 4126 2474 Size: 1652 3968 HaeII r_GCGC'y Cuts at: 1678 4767 1678 Size: 3089 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2581 2613 4261 4293 4335 Size: 32 135 32 1009 32 1648 32 42 Cuts at: 4335 4367 1373 Size: 32 2626 Fragments arranged by size: 2626 1648 1009 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2221 4734 4918 1305 Size: 916 2513 184 2007 Fragments arranged by size: 2513 2007 916 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3798 Fragments arranged by size: 3798 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2552 3578 3937 4096 518 Size: 648 843 543 1026 359 159 2042 Fragments arranged by size: 2042 1026 843 648 543 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2335 2552 2701 2824 2885 3036 3713 Size: 483 246 217 149 123 61 151 677 Cuts at: 3713 4861 5106 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 483 246 245 217 151 149 123 61 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4129 Fragments arranged by size: 4129 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2978 4523 1132 Size: 97 1749 1545 2229 Fragments arranged by size: 2229 1749 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 2491 1108 Size: 1383 4237 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2260 2785 2134 Size: 126 525 4969 Fragments arranged by size: 4969 525 126 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PstI C_TGCA'G Cuts at: 830 2343 2808 2907 2972 830 Size: 1513 465 99 65 3478 Fragments arranged by size: 3478 1513 465 99 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 PvuII CAG'CTG Cuts at: 2089 2335 2552 2701 2885 2089 Size: 246 217 149 184 4824 Fragments arranged by size: 4824 246 217 184 149 SacI G_AGCT'C Cuts at: 2301 2301 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SanDI GG'GwC_CC Cuts at: 2134 2134 Size: 5620 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2339 2804 2903 2968 4103 4784 4975 Size: 219 1294 465 99 65 1135 681 191 Cuts at: 4975 826 Size: 1471 Fragments arranged by size: 1471 1294 1135 681 465 219 191 99 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2689 2947 1872 Size: 817 258 4545 Fragments arranged by size: 4545 817 258 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 3724 4625 4887 820 Size: 197 926 225 1556 901 262 1553 Fragments arranged by size: 1556 1553 926 901 262 225 197 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2978 1132 Size: 1846 3774 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5620 StyI C'CwwG_G Cuts at: 513 1065 2004 3059 513 Size: 552 939 1055 3074 Fragments arranged by size: 3074 1055 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2408 2498 2683 2711 3647 3664 3817 Size: 1255 828 90 185 28 936 17 153 Cuts at: 3817 4002 4447 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 936 828 445 185 185 153 90 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2058 2271 3866 Size: 274 80 33 51 1443 81 213 1595 Cuts at: 3866 5578 96 Size: 1712 138 Fragments arranged by size: 1712 1595 1443 274 213 138 81 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5580 5612 5580 Size: 32 5588 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2743 2842 2743 Size: 99 5521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2687 2945 1870 Size: 817 258 4545 Fragments arranged by size: 4545 817 258 XmnI GAAnn'nnTTC Cuts at: 3749 3749 Size: 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI