Restriction map for
pFN21AE0706
(Linear) (Six-base) MAPSORT of: KIEE0706.seq Check: 4916 from: 1 to: 1221
KIEE0706.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AleI CACnn'nnGTG
Cuts at: 0 368 1221
Size: 368 853
ApaI G_GGCC'C
Cuts at: 0 1150 1221
Size: 1150 71
ApoI r'AATT_y
Cuts at: 0 169 356 1221
Size: 169 187 865
Fragments arranged by size:
865 187 169
AvaI C'yCGr_G
Cuts at: 0 9 149 260 458 569 946 1221
Size: 9 140 111 198 111 377 275
Fragments arranged by size:
377 275 198 140 111 111 9
BanI G'GyrC_C
Cuts at: 0 13 604 708 853 1221
Size: 13 591 104 145 368
Fragments arranged by size:
591 368 145 104 13
BanII G_rGCy'C
Cuts at: 0 386 765 1150 1221
Size: 386 379 385 71
Fragments arranged by size:
386 385 379 71
BbeI G_GCGC'C
Cuts at: 0 17 1221
Size: 17 1204
BfrBI ATG'CAT
Cuts at: 0 519 1221
Size: 519 702
BglI GCCn_nnn'nGGC
Cuts at: 0 675 1221
Size: 675 546
BlpI GC'TnA_GC
Cuts at: 0 563 1019 1221
Size: 563 456 202
Fragments arranged by size:
563 456 202
Bme1580I G_kGCm'C
Cuts at: 0 635 858 1150 1221
Size: 635 223 292 71
Fragments arranged by size:
635 292 223 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 833 1221
Size: 75 758 388
Fragments arranged by size:
758 388 75
Bpu10I CC'TnA_GC
Cuts at: 0 385 1221
Size: 385 836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1086 1221
Size: 1086 135
BsaAI yAC'GTr
Cuts at: 0 674 1221
Size: 674 547
BsaHI Gr'CG_yC
Cuts at: 0 14 440 1221
Size: 14 426 781
Fragments arranged by size:
781 426 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 92 122 1221
Size: 92 30 1099
Fragments arranged by size:
1099 92 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 773 920 1221
Size: 773 147 301
Fragments arranged by size:
773 301 147
BseYI C'CCAG_C
Cuts at: 0 650 1096 1221
Size: 650 446 125
Fragments arranged by size:
650 446 125
BsiEI CG_ry'CG
Cuts at: 0 289 1221
Size: 289 932
BsiHKAI G_wGCw'C
Cuts at: 0 61 386 1221
Size: 61 325 835
Fragments arranged by size:
835 325 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 986 1221
Size: 986 235
Bsp1286I G_dGCh'C
Cuts at: 0 61 386 635 765 858 1150 1221
Size: 61 325 249 130 93 292 71
Fragments arranged by size:
325 292 249 130 93 71 61
BspHI T'CATG_A
Cuts at: 0 122 428 1221
Size: 122 306 793
Fragments arranged by size:
793 306 122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1039 1221
Size: 1039 182
BsrFI r'CCGG_y
Cuts at: 0 711 1156 1221
Size: 711 445 65
Fragments arranged by size:
711 445 65
Bsu36I CC'TnA_GG
Cuts at: 0 842 1221
Size: 842 379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 170 205 685 720 1221
Size: 170 35 480 35 501
Fragments arranged by size:
501 480 170 35 35
DraI TTT'AAA
Cuts at: 0 174 1221
Size: 174 1047
DraIII CAC_nnn'GTG
Cuts at: 0 1194 1221
Size: 1194 27
DrdI GACnn_nn'nnGTC
Cuts at: 0 508 1221
Size: 508 713
EaeI y'GGCC_r
Cuts at: 0 203 228 286 1221
Size: 203 25 58 935
Fragments arranged by size:
935 203 58 25
EagI C'GGCC_G
Cuts at: 0 286 1221
Size: 286 935
EarI CTCTTCn'nnn_
Cuts at: 0 40 750 1221
Size: 40 710 471
Fragments arranged by size:
710 471 40
EcoICRI GAG'CTC
Cuts at: 0 384 1221
Size: 384 837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 833 1221
Size: 75 758 388
Fragments arranged by size:
758 388 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 812 1221
Size: 812 409
EcoO109I rG'GnC_Cy
Cuts at: 0 310 455 1123 1146 1147 1221
Size: 310 145 668 23 1 74
Fragments arranged by size:
668 310 145 74 23 1
EcoRI G'AATT_C
Cuts at: 0 356 1221
Size: 356 865
FspI TGC'GCA
Cuts at: 0 79 1221
Size: 79 1142
HaeII r_GCGC'y
Cuts at: 0 17 592 1221
Size: 17 575 629
Fragments arranged by size:
629 575 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 1221
Size: 478 32 711
Fragments arranged by size:
711 478 32
HincII GTy'rAC
Cuts at: 0 513 1221
Size: 513 708
KasI G'GCGC_C
Cuts at: 0 13 1221
Size: 13 1208
MfeI C'AATT_G
Cuts at: 0 366 1221
Size: 366 855
MscI TGG'CCA
Cuts at: 0 205 1221
Size: 205 1016
MslI CAynn'nnrTG
Cuts at: 0 368 1221
Size: 368 853
MspA1I CmG'CkG
Cuts at: 0 1166 1221
Size: 1166 55
NaeI GCC'GGC
Cuts at: 0 713 1158 1221
Size: 713 445 63
Fragments arranged by size:
713 445 63
NarI GG'CG_CC
Cuts at: 0 14 1221
Size: 14 1207
NgoMIV G'CCGG_C
Cuts at: 0 711 1156 1221
Size: 711 445 65
Fragments arranged by size:
711 445 65
NsiI A_TGCA'T
Cuts at: 0 521 1221
Size: 521 700
NspI r_CATG'y
Cuts at: 0 519 1221
Size: 519 702
PasI CC'CwG_GG
Cuts at: 0 831 1221
Size: 831 390
PflMI CCAn_nnn'nTGG
Cuts at: 0 614 650 1221
Size: 614 36 571
Fragments arranged by size:
614 571 36
PfoI T'CCnGG_A
Cuts at: 0 810 1221
Size: 810 411
PmlI CAC'GTG
Cuts at: 0 674 1221
Size: 674 547
PpuMI rG'GwC_Cy
Cuts at: 0 310 1123 1221
Size: 310 813 98
Fragments arranged by size:
813 310 98
PspOMI G'GGCC_C
Cuts at: 0 1146 1221
Size: 1146 75
PstI C_TGCA'G
Cuts at: 0 753 1221
Size: 753 468
SacI G_AGCT'C
Cuts at: 0 386 1221
Size: 386 835
SanDI GG'GwC_CC
Cuts at: 0 1123 1221
Size: 1123 98
SapI GCTCTTCn'nnn_
Cuts at: 0 40 1221
Size: 40 1181
SbfI CC_TGCA'GG
Cuts at: 0 753 1221
Size: 753 468
SfcI C'TryA_G
Cuts at: 0 749 1221
Size: 749 472
SfoI GGC'GCC
Cuts at: 0 15 1221
Size: 15 1206
SgrAI Cr'CCGG_yG
Cuts at: 0 1156 1221
Size: 1156 65
SmaI CCC'GGG
Cuts at: 0 262 948 1221
Size: 262 686 273
Fragments arranged by size:
686 273 262
SmlI C'TyrA_G
Cuts at: 0 149 1065 1221
Size: 149 916 156
Fragments arranged by size:
916 156 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 696 1221
Size: 696 525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 692 724 1221
Size: 692 32 497
Fragments arranged by size:
692 497 32
XhoI C'TCGA_G
Cuts at: 0 149 1221
Size: 149 1072
XmaI C'CCGG_G
Cuts at: 0 260 946 1221
Size: 260 686 275
Fragments arranged by size:
686 275 260
XmnI GAAnn'nnTTC
Cuts at: 0 34 1221
Size: 34 1187
Enzymes that do cut:
AleI ApaI ApoI AvaI BanI BanII BbeI BfrBI
BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrFI Bsu36I CspCI DraI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII KasI MfeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PasI PflMI PfoI
PmlI PpuMI PspOMI PstI SacI SanDI SapI SbfI
SfcI SfoI SgrAI SmaI SmlI TaqII TstI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BglII BmgBI BmrI BmtI BplI BsaI
BsaBI BsaWI BsgI BsiWI BsmI BspEI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI EciI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MluI MmeI NcoI
NdeI NheI NotI NruI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI ScaI SexAI SfiI SgfI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI Tth111I XbaI
XcmI ZraI