(Circular) (Six-base) MAPSORT of: pFN21AE0685.seq Check: 1058 from: 1 to: 5545 pFN21AE0685 5545 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3355 278 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2888 1937 Size: 951 4594 Acc65I G'GTAC_C Cuts at: 2866 2866 Size: 5545 AclI AA'CG_TT Cuts at: 1563 3672 4045 1563 Size: 2109 373 3063 Fragments arranged by size: 3063 2109 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2429 3608 4992 5260 1632 Size: 797 1179 1384 268 1917 Fragments arranged by size: 1917 1384 1179 797 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5348 AflIII A'CryG_T Cuts at: 1203 2526 4444 5421 1203 Size: 1323 1918 977 1327 Fragments arranged by size: 1918 1327 1323 977 AgeI A'CCGG_T Cuts at: 4376 4376 Size: 5545 AhdI GACnn_n'nnGTC Cuts at: 4274 4274 Size: 5545 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5545 AlwNI CAG_nnn'CTG Cuts at: 2489 3288 4860 2489 Size: 799 1572 3174 Fragments arranged by size: 3174 1572 799 ApaI G_GGCC'C Cuts at: 2417 2824 2417 Size: 407 5138 ApaLI G'TGCA_C Cuts at: 3602 4758 3602 Size: 1156 4389 ApoI r'AATT_y Cuts at: 1493 2831 2854 3080 3232 5341 1493 Size: 1338 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 1338 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 4099 160 Size: 872 3067 1606 Fragments arranged by size: 3067 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 AvaI C'yCGr_G Cuts at: 1870 1943 2870 1870 Size: 73 927 4545 Fragments arranged by size: 4545 927 73 BamHI G'GATC_C Cuts at: 2875 3242 2875 Size: 367 5178 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 2866 4321 618 Size: 325 217 627 366 713 1455 1842 Fragments arranged by size: 1842 1455 713 627 366 325 217 BanII G_rGCy'C Cuts at: 1391 2303 2417 2824 2864 1391 Size: 912 114 407 40 4072 Fragments arranged by size: 4072 912 407 114 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4574 BbvCI CC'TCA_GC Cuts at: 2559 2559 Size: 5545 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2767 2801 3736 3770 1903 Size: 34 830 34 935 34 3678 Fragments arranged by size: 3678 935 830 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2063 3437 4647 2063 Size: 1374 1210 2961 Fragments arranged by size: 2961 1374 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2067 4156 Size: 107 122 71 631 605 337 58 2089 Cuts at: 4156 136 Size: 1525 Fragments arranged by size: 2089 1525 631 605 337 122 107 71 58 BglII A'GATC_T Cuts at: 5540 5540 Size: 5545 BlpI GC'TnA_GC Cuts at: 2962 2962 Size: 5545 Bme1580I G_kGCm'C Cuts at: 1790 2205 2417 2496 2824 3606 4762 1790 Size: 415 212 79 328 782 1156 2573 Fragments arranged by size: 2573 1156 782 415 328 212 79 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 3276 4234 Size: 416 53 83 399 24 658 1201 958 Cuts at: 4234 442 Size: 1753 Fragments arranged by size: 1753 1201 958 658 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5545 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2702 4205 1239 Size: 1463 1503 2579 Fragments arranged by size: 2579 1503 1463 Bpu10I CC'TnA_GC Cuts at: 2559 2571 2559 Size: 12 5533 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3670 4535 4833 3670 Size: 865 298 4382 Fragments arranged by size: 4382 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4208 882 Size: 617 2709 2219 Fragments arranged by size: 2709 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2365 3336 493 Size: 711 1161 971 2702 Fragments arranged by size: 2702 1161 971 711 BsaBI GATnn'nnATC Cuts at: 5539 5539 Size: 5545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3352 3734 4369 275 Size: 53 83 186 936 1819 382 635 1451 Fragments arranged by size: 1819 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3978 4376 4650 4797 5208 3978 Size: 398 274 147 411 4315 Fragments arranged by size: 4315 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2213 2243 2300 2330 1178 Size: 30 165 30 810 30 57 30 4393 Fragments arranged by size: 4393 810 165 57 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2234 2597 2600 2618 2804 1186 Size: 1048 363 3 18 186 3927 Fragments arranged by size: 3927 1048 363 186 18 3 BseYI C'CCAG_C Cuts at: 2057 2610 2823 4748 5244 2057 Size: 553 213 1925 496 2358 Fragments arranged by size: 2358 1925 553 496 213 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2197 1163 Size: 573 461 4511 Fragments arranged by size: 4511 573 461 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2840 2995 3756 4784 1540 Size: 275 186 839 155 761 1028 2301 Fragments arranged by size: 2301 1028 839 761 275 186 155 BsiHKAI G_wGCw'C Cuts at: 2137 2649 3606 3691 4762 2137 Size: 512 957 85 1071 2920 Fragments arranged by size: 2920 1071 957 512 85 BsiWI C'GTAC_G Cuts at: 2841 2841 Size: 5545 BsmI GAATG_Cn' Cuts at: 2457 3056 3149 2457 Size: 599 93 4853 Fragments arranged by size: 4853 599 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2686 2686 Size: 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2205 2303 2417 2496 2649 2824 Size: 399 347 68 98 114 79 153 175 Cuts at: 2824 2864 3606 3691 4762 1391 Size: 40 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 347 175 153 114 98 85 79 68 40 BspEI T'CCGG_A Cuts at: 5208 5208 Size: 5545 BspHI T'CATG_A Cuts at: 3434 5164 3434 Size: 1730 3815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2900 844 Size: 2056 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3432 1394 Size: 429 1609 3507 Fragments arranged by size: 3507 1609 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4040 4214 65 Size: 1183 2792 174 1396 Fragments arranged by size: 2792 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2634 4189 4376 1700 Size: 110 824 1555 187 2869 Fragments arranged by size: 2869 1555 824 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5545 BssHII G'CGCG_C Cuts at: 1925 2012 1925 Size: 87 5458 BssSI C'ACGA_G Cuts at: 3605 4617 3605 Size: 1012 4533 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5545 BstBI TT'CG_AA Cuts at: 4349 4349 Size: 5545 BstEII G'GTnAC_C Cuts at: 1184 2620 1184 Size: 1436 4109 BstXI CCAn_nnnn'nTGG Cuts at: 2065 2065 Size: 5545 BstYI r'GATC_y Cuts at: 1972 2875 3242 3627 3644 5096 5182 5194 5540 Size: 903 367 385 17 1452 86 12 346 Cuts at: 5540 1972 Size: 1977 Fragments arranged by size: 1977 1452 903 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2502 2510 2502 Size: 8 5537 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4054 Fragments arranged by size: 4054 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4806 Fragments arranged by size: 4806 683 56 BtsI GCAGTG_nn' Cuts at: 3061 3855 3875 3061 Size: 794 20 4731 Fragments arranged by size: 4731 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5510 DraI TTT'AAA Cuts at: 2849 2849 Size: 5545 DraIII CAC_nnn'GTG Cuts at: 2446 2446 Size: 5545 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4552 809 Size: 507 3236 1802 Fragments arranged by size: 3236 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2992 3881 5510 Size: 54 1425 50 275 18 1162 889 1629 Cuts at: 5510 8 Size: 43 Fragments arranged by size: 1629 1425 1162 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2992 1537 Size: 275 1180 4090 Fragments arranged by size: 4090 1180 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 2444 3475 1353 Size: 818 273 1031 3423 Fragments arranged by size: 3423 1031 818 273 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4132 4506 4652 387 Size: 1064 138 2543 374 146 1280 Fragments arranged by size: 2543 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2429 2702 3608 4205 4992 5260 1239 Size: 393 797 273 906 597 787 268 1524 Fragments arranged by size: 1524 906 797 787 597 393 273 268 EcoO109I rG'GnC_Cy Cuts at: 2038 2413 2414 2506 2593 2038 Size: 375 1 92 87 4990 Fragments arranged by size: 4990 375 92 87 1 EcoRI G'AATT_C Cuts at: 2854 3232 2854 Size: 378 5167 FspI TGC'GCA Cuts at: 2777 4051 2777 Size: 1274 4271 HaeII r_GCGC'y Cuts at: 1678 4692 1678 Size: 3014 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2300 2332 2746 Size: 32 135 32 641 32 55 32 414 Cuts at: 2746 2778 4186 4218 4260 4292 1373 Size: 32 1408 32 42 32 2626 Fragments arranged by size: 2626 1408 641 414 135 55 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2889 3135 669 Size: 988 281 951 246 3079 Fragments arranged by size: 3079 988 951 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5545 HpaI GTT'AAC Cuts at: 3135 3135 Size: 5545 KpnI G_GTAC'C Cuts at: 2870 2870 Size: 5545 MfeI C'AATT_G Cuts at: 3144 3144 Size: 5545 MluI A'CGCG_T Cuts at: 5421 5421 Size: 5545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2860 4659 4843 1305 Size: 1555 1799 184 2007 Fragments arranged by size: 2007 1799 1555 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3723 Fragments arranged by size: 3723 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2531 3503 3862 4021 518 Size: 648 1365 972 359 159 2042 Fragments arranged by size: 2042 1365 972 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2396 2773 2961 3638 4786 5031 1606 Size: 790 377 188 677 1148 245 2120 Fragments arranged by size: 2120 1148 790 677 377 245 188 NaeI GCC'GGC Cuts at: 2636 2636 Size: 5545 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4054 Fragments arranged by size: 4054 939 552 NdeI CA'TA_TG Cuts at: 387 2542 387 Size: 2155 3390 NgoMIV G'CCGG_C Cuts at: 2634 2634 Size: 5545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5545 NotI GC'GGCC_GC Cuts at: 2992 2992 Size: 5545 NspI r_CATG'y Cuts at: 1132 1229 2903 4448 1132 Size: 97 1674 1545 2229 Fragments arranged by size: 2229 1674 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5545 PciI A'CATG_T Cuts at: 4444 4444 Size: 5545 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5545 PmeI GTTT'AAAC Cuts at: 2849 2849 Size: 5545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3614 3646 5127 5159 3614 Size: 32 1481 32 4000 Fragments arranged by size: 4000 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2038 2593 2038 Size: 555 4990 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5545 PsiI TTA'TAA Cuts at: 3115 3115 Size: 5545 PspOMI G'GGCC_C Cuts at: 2413 2820 2413 Size: 407 5138 PstI C_TGCA'G Cuts at: 830 2897 830 Size: 2067 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5545 PvuII CAG'CTG Cuts at: 2773 2773 Size: 5545 SalI G'TCGA_C Cuts at: 1936 2887 1936 Size: 951 4594 SanDI GG'GwC_CC Cuts at: 2038 2593 2038 Size: 555 4990 SbfI CC_TGCA'GG Cuts at: 2897 2897 Size: 5545 ScaI AGT'ACT Cuts at: 3793 5505 3793 Size: 1712 3833 SfcI C'TryA_G Cuts at: 826 1045 2265 2893 4028 4709 4900 826 Size: 219 1220 628 1135 681 191 1471 Fragments arranged by size: 1471 1220 1135 681 628 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5545 SmaI CCC'GGG Cuts at: 1872 2872 1872 Size: 1000 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3649 4550 4812 820 Size: 197 926 1706 901 262 1553 Fragments arranged by size: 1706 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3336 493 Size: 2843 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5545 SphI G_CATG'C Cuts at: 1132 2903 1132 Size: 1771 3774 SspI AAT'ATT Cuts at: 5 52 3469 5 Size: 47 3417 2081 Fragments arranged by size: 3417 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5545 StyI C'CwwG_G Cuts at: 513 1065 2004 2400 2577 2984 513 Size: 552 939 396 177 407 3074 Fragments arranged by size: 3074 939 552 407 396 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2602 3572 3589 3742 3927 4372 325 Size: 1255 1022 970 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1022 970 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3791 5503 96 Size: 274 80 33 51 1443 1814 1712 138 Fragments arranged by size: 1814 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5505 5537 5505 Size: 32 5513 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5545 XbaI T'CTAG_A Cuts at: 2881 2881 Size: 5545 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2381 2381 Size: 5545 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5545 XmaI C'CCGG_G Cuts at: 1870 2870 1870 Size: 1000 4545 XmnI GAAnn'nnTTC Cuts at: 3674 3674 Size: 5545 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3353 276 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI