Restriction map for
pFN21AB6823
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BanI BbeI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MboII
MfeI MluI MroI MseI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AlwNI 369 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 68 GTGCTC G[AT]GC[AT]^C
AvaII 234 GGACC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BamHI 47 GGATCC G^GATCC
BanII 482 GAGCCC G[GA]GC[CT]^C
BbrPI 420 CACGTG CAC^GTG
BsaAI 420 CACGTG [CT]AC^GT[GA]
BsaBI 269 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 11 CCAAGG C^C[ACGT][ACGT]GG
BsgI 389 GTGCAG GTGCAG(16/14)
BsiHKAI 68 GTGCTC G[AT]GC[AT]^C
BspMI 131 ACCTGC ACCTGC(4/8)
BsrDI 313 CATTGC GCAATG(2/0)
BstAPI 79 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 467 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 42 GCGC GCG^C
DraIII 472 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoT14I 11 CCAAGG C^C[AT][AT]GG
HhaI 42 GCGC GCG^C
HinP1I 42 GCGC G^CGC
MamI 269 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 306 CAGCTG C[AC]G^C[GT]G
PleI 325 GAGTC GAGTC(4/5)
PmaCI 420 CACGTG CAC^GTG
PmlI 420 CACGTG CAC^GTG
PvuII 306 CAGCTG CAG^CTG
StyI 11 CCAAGG C^C[AT][AT]GG
TaqI 323 TCGA T^CGA
TfiI 320 GATTC G^A[AT]TC
TthHB8I 323 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BmyI 68 GTGCTC G[AGT]GC[ACT]^C
482 GAGCCC
BsiYI 172 CCAAAGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
237 CCATGCAAAGG
BslI 172 CCAAAGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
237 CCATGCAAAGG
BsmAI 260 GAGAC GTCTC(1/5)
429 GTCTC
BsmFI 180 GGGAC GGGAC(10/14)
233 GGGAC
Bsp1286I 68 GTGCTC G[AGT]GC[ACT]^C
482 GAGCCC
BsrI 293 ACTGG ACTGG(1/-1)
65 CCAGT
BssKI 51 CCAGG ^CC[ACGT]GG
457 CCAGG
BstNI 51 CCAGG CC^[AT]GG
457 CCAGG
BstYI 47 GGATCC [GA]^GATC[CT]
444 GGATCT
Cfr13I 234 GGACC G^G[ACGT]CC
296 GGGCC
DdeI 108 CTCAG C^T[ACGT]AG
154 CTGAG
DpnII 48 GATC ^GATC
445 GATC
EcoRII 51 CCAGG ^CC[AT]GG
457 CCAGG
HaeIII 3 GGCC GG^CC
297 GGCC
HinfI 320 GATTC G^A[ACGT]TC
325 GAGTC
HphI 382 GGTGA GGTGA(8/7)
114 TCACC
MboI 48 GATC ^GATC
445 GATC
MflI 47 GGATCC [GA]^GATC[CT]
444 GGATCT
MvaI 51 CCAGG CC^[AT]GG
457 CCAGG
NdeII 48 GATC ^GATC
445 GATC
PstI 77 CTGCAG CTGCA^G
253 CTGCAG
Sau3AI 48 GATC ^GATC
445 GATC
Sau96I 234 GGACC G^G[ACGT]CC
296 GGGCC
ScrFI 51 CCAGG CC^[ACGT]GG
457 CCAGG
SfaNI 31 GCATC GCATC(5/9)
453 GCATC
SfcI 77 CTGCAG C^T[GA][CT]AG
253 CTGCAG
Tsp45I 16 GTCAC ^GT[GC]AC
327 GTCAC
XhoII 47 GGATCC [GA]^GATC[CT]
444 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI AlwI BbvI BstF5I Fnu4HI
FokI ItaI MaeII MaeIII MnlI
MwoI NlaIII NlaIV TseI TspRI