Restriction map for
pFN21AB6841
(Linear) (Six-base) MAPSORT of: KIBB6841.seq Check: 3171 from: 1 to: 516
KIBB6841.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 302 377 516
Size: 302 75 139
Fragments arranged by size:
302 139 75
AflIII A'CryG_T
Cuts at: 0 7 516
Size: 7 509
AhdI GACnn_n'nnGTC
Cuts at: 0 459 516
Size: 459 57
AlwNI CAG_nnn'CTG
Cuts at: 0 158 378 516
Size: 158 220 138
Fragments arranged by size:
220 158 138
AvaI C'yCGr_G
Cuts at: 0 299 403 499 516
Size: 299 104 96 17
Fragments arranged by size:
299 104 96 17
BanI G'GyrC_C
Cuts at: 0 418 477 516
Size: 418 59 39
Fragments arranged by size:
418 59 39
BanII G_rGCy'C
Cuts at: 0 212 404 500 516
Size: 212 192 96 16
Fragments arranged by size:
212 192 96 16
BbeI G_GCGC'C
Cuts at: 0 422 481 516
Size: 422 59 35
Fragments arranged by size:
422 59 35
BbvCI CC'TCA_GC
Cuts at: 0 322 516
Size: 322 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 256 290 516
Size: 256 34 226
Fragments arranged by size:
256 226 34
BglI GCCn_nnn'nGGC
Cuts at: 0 475 516
Size: 475 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 516
Size: 403 113
Bpu10I CC'TnA_GC
Cuts at: 0 322 516
Size: 322 194
BsaI GGTCTCn'nnnn_
Cuts at: 0 281 516
Size: 281 235
BsaHI Gr'CG_yC
Cuts at: 0 128 419 478 516
Size: 128 291 59 38
Fragments arranged by size:
291 128 59 38
BseYI C'CCAG_C
Cuts at: 0 137 428 487 516
Size: 137 291 59 29
Fragments arranged by size:
291 137 59 29
BsiEI CG_ry'CG
Cuts at: 0 416 516
Size: 416 100
BsmI GAATG_Cn'
Cuts at: 0 448 516
Size: 448 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 328 516
Size: 328 188
Bsp1286I G_dGCh'C
Cuts at: 0 212 404 500 516
Size: 212 192 96 16
Fragments arranged by size:
212 192 96 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 377 516
Size: 302 75 139
Fragments arranged by size:
302 139 75
BsrBI CCG'CTC
Cuts at: 0 243 516
Size: 243 273
BsrFI r'CCGG_y
Cuts at: 0 15 415 516
Size: 15 400 101
Fragments arranged by size:
400 101 15
BssHII G'CGCG_C
Cuts at: 0 148 277 516
Size: 148 129 239
Fragments arranged by size:
239 148 129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 158 180 252 378 516
Size: 158 22 72 126 138
Fragments arranged by size:
158 138 126 72 22
Bsu36I CC'TnA_GG
Cuts at: 0 106 516
Size: 106 410
BtgI C'CryG_G
Cuts at: 0 91 516
Size: 91 425
EaeI y'GGCC_r
Cuts at: 0 413 516
Size: 413 103
EagI C'GGCC_G
Cuts at: 0 413 516
Size: 413 103
EarI CTCTTCn'nnn_
Cuts at: 0 477 516
Size: 477 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 454 516
Size: 34 420 62
Fragments arranged by size:
420 62 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 516
Size: 403 113
EcoO109I rG'GnC_Cy
Cuts at: 0 74 99 516
Size: 74 25 417
Fragments arranged by size:
417 74 25
FspI TGC'GCA
Cuts at: 0 49 162 516
Size: 49 113 354
Fragments arranged by size:
354 113 49
HaeII r_GCGC'y
Cuts at: 0 203 321 390 422 481 516
Size: 203 118 69 32 59 35
Fragments arranged by size:
203 118 69 59 35 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 516
Size: 445 32 39
Fragments arranged by size:
445 39 32
KasI G'GCGC_C
Cuts at: 0 418 477 516
Size: 418 59 39
Fragments arranged by size:
418 59 39
MluI A'CGCG_T
Cuts at: 0 7 516
Size: 7 509
MslI CAynn'nnrTG
Cuts at: 0 168 516
Size: 168 348
MspA1I CmG'CkG
Cuts at: 0 93 155 192 249 516
Size: 93 62 37 57 267
Fragments arranged by size:
267 93 62 57 37
NaeI GCC'GGC
Cuts at: 0 417 516
Size: 417 99
NarI GG'CG_CC
Cuts at: 0 419 478 516
Size: 419 59 38
Fragments arranged by size:
419 59 38
NgoMIV G'CCGG_C
Cuts at: 0 415 516
Size: 415 101
PasI CC'CwG_GG
Cuts at: 0 60 516
Size: 60 456
PpuMI rG'GwC_Cy
Cuts at: 0 74 99 516
Size: 74 25 417
Fragments arranged by size:
417 74 25
PspXI vC'TCGA_Gb
Cuts at: 0 403 516
Size: 403 113
PstI C_TGCA'G
Cuts at: 0 374 516
Size: 374 142
SacII CC_GC'GG
Cuts at: 0 94 516
Size: 94 422
SanDI GG'GwC_CC
Cuts at: 0 74 99 516
Size: 74 25 417
Fragments arranged by size:
417 74 25
SbfI CC_TGCA'GG
Cuts at: 0 374 516
Size: 374 142
ScaI AGT'ACT
Cuts at: 0 178 516
Size: 178 338
SfcI C'TryA_G
Cuts at: 0 370 516
Size: 370 146
SfoI GGC'GCC
Cuts at: 0 420 479 516
Size: 420 59 37
Fragments arranged by size:
420 59 37
SmaI CCC'GGG
Cuts at: 0 301 516
Size: 301 215
SmlI C'TyrA_G
Cuts at: 0 403 516
Size: 403 113
StuI AGG'CCT
Cuts at: 0 394 469 516
Size: 394 75 47
Fragments arranged by size:
394 75 47
StyI C'CwwG_G
Cuts at: 0 464 516
Size: 464 52
TatI w'GTAC_w
Cuts at: 0 176 516
Size: 176 340
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 118 150 516
Size: 118 32 366
Fragments arranged by size:
366 118 32
XhoI C'TCGA_G
Cuts at: 0 403 516
Size: 403 113
XmaI C'CCGG_G
Cuts at: 0 299 516
Size: 299 217
Enzymes that do cut:
AarI AflIII AhdI AlwNI AvaI BanI BanII BbeI
BbvCI BcgI BglI BpmI Bpu10I BsaI BsaHI BseYI
BsiEI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BstAPI Bsu36I BtgI EaeI EagI EarI EciI Eco57MI
EcoO109I FspI HaeII Hin4I KasI MluI MslI MspA1I
NaeI NarI NgoMIV PasI PpuMI PspXI PstI SacII
SanDI SbfI ScaI SfcI SfoI SmaI SmlI StuI
StyI TatI TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsiWI
BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI
BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MfeI MmeI MscI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PvuI PvuII RsrII SacI SalI SapI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XcmI XmnI ZraI