(Circular) (Six-base) MAPSORT of: pFN21AB6703.seq Check: 9808 from: 1 to: 4924 pFN21AB6703 4924 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2734 278 Size: 53 83 186 936 1198 2468 Fragments arranged by size: 2468 1198 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2267 1937 Size: 330 4594 Acc65I G'GTAC_C Cuts at: 2111 2245 2111 Size: 134 4790 AclI AA'CG_TT Cuts at: 1563 3051 3424 1563 Size: 1488 373 3063 Fragments arranged by size: 3063 1488 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2156 2987 4371 4639 1632 Size: 524 831 1384 268 1917 Fragments arranged by size: 1917 1384 831 524 268 AflII C'TTAA_G Cuts at: 820 1017 2084 820 Size: 197 1067 3660 Fragments arranged by size: 3660 1067 197 AflIII A'CryG_T Cuts at: 1203 2166 3823 4800 1203 Size: 963 1657 977 1327 Fragments arranged by size: 1657 1327 977 963 AgeI A'CCGG_T Cuts at: 3755 3755 Size: 4924 AhdI GACnn_n'nnGTC Cuts at: 2167 3653 2167 Size: 1486 3438 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4924 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4924 AlwNI CAG_nnn'CTG Cuts at: 2667 4239 2667 Size: 1572 3352 ApaLI G'TGCA_C Cuts at: 2981 4137 2981 Size: 1156 3768 ApoI r'AATT_y Cuts at: 1493 2233 2459 2611 4720 1493 Size: 740 226 152 2109 1697 Fragments arranged by size: 2109 1697 740 226 152 AseI AT'TA_AT Cuts at: 160 1032 3478 160 Size: 872 2446 1606 Fragments arranged by size: 2446 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4924 AvaI C'yCGr_G Cuts at: 1870 1943 2249 1870 Size: 73 306 4545 Fragments arranged by size: 4545 306 73 BamHI G'GATC_C Cuts at: 2254 2621 2254 Size: 367 4557 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2245 3700 618 Size: 325 217 627 324 134 1455 1842 Fragments arranged by size: 1842 1455 627 325 324 217 134 BanII G_rGCy'C Cuts at: 1391 2243 1391 Size: 852 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3953 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3115 3149 1903 Size: 34 1178 34 3678 Fragments arranged by size: 3678 1178 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2816 4026 2816 Size: 1210 3714 BclI T'GATC_A Cuts at: 1548 1548 Size: 4924 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3535 136 Size: 107 122 71 631 605 1863 1525 Fragments arranged by size: 1863 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4919 4919 Size: 4924 BlpI GC'TnA_GC Cuts at: 2341 2341 Size: 4924 Bme1580I G_kGCm'C Cuts at: 1790 2985 4141 1790 Size: 1195 1156 2573 Fragments arranged by size: 2573 1195 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4924 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2655 3613 442 Size: 416 53 83 399 24 1238 958 1753 Fragments arranged by size: 1753 1238 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4924 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 3584 1239 Size: 989 1356 2579 Fragments arranged by size: 2579 1356 989 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3049 3914 4212 3049 Size: 865 298 3761 Fragments arranged by size: 3761 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3587 882 Size: 617 2088 2219 Fragments arranged by size: 2219 2088 617 BsaAI yAC'GTr Cuts at: 493 1204 2167 2715 493 Size: 711 963 548 2702 Fragments arranged by size: 2702 963 711 548 BsaBI GATnn'nnATC Cuts at: 4918 4918 Size: 4924 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2731 3113 3748 275 Size: 53 83 186 936 1198 382 635 1451 Fragments arranged by size: 1451 1198 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3357 3755 4029 4176 4587 3357 Size: 398 274 147 411 3694 Fragments arranged by size: 3694 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4699 Fragments arranged by size: 4699 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4924 BseYI C'CCAG_C Cuts at: 4127 4623 4127 Size: 496 4428 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2029 2374 3135 4163 1540 Size: 275 186 28 345 761 1028 2301 Fragments arranged by size: 2301 1028 761 345 275 186 28 BsiHKAI G_wGCw'C Cuts at: 2985 3070 4141 2985 Size: 85 1071 3768 Fragments arranged by size: 3768 1071 85 BsmI GAATG_Cn' Cuts at: 2435 2528 2435 Size: 93 4831 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2243 2985 3070 4141 1391 Size: 399 453 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 399 85 BspEI T'CCGG_A Cuts at: 4587 4587 Size: 4924 BspHI T'CATG_A Cuts at: 2813 4543 2813 Size: 1730 3194 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2279 844 Size: 1435 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2811 1394 Size: 429 988 3507 Fragments arranged by size: 3507 988 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3419 3593 65 Size: 1183 2171 174 1396 Fragments arranged by size: 2171 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3568 3755 1700 Size: 110 1758 187 2869 Fragments arranged by size: 2869 1758 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4924 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4924 BssSI C'ACGA_G Cuts at: 2984 3996 2984 Size: 1012 3912 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4924 BstBI TT'CG_AA Cuts at: 3728 3728 Size: 4924 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4924 BstYI r'GATC_y Cuts at: 1972 2254 2621 3006 3023 4475 4561 4573 4919 Size: 282 367 385 17 1452 86 12 346 Cuts at: 4919 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 282 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4372 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4185 Fragments arranged by size: 4185 683 56 BtsI GCAGTG_nn' Cuts at: 2440 3234 3254 2440 Size: 794 20 4110 Fragments arranged by size: 4110 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4924 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4889 DraI TTT'AAA Cuts at: 2228 2228 Size: 4924 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3931 809 Size: 507 2615 1802 Fragments arranged by size: 2615 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2371 3260 4889 Size: 54 1425 50 275 18 541 889 1629 Cuts at: 4889 8 Size: 43 Fragments arranged by size: 1629 1425 889 541 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2371 1537 Size: 275 559 4090 Fragments arranged by size: 4090 559 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2854 1353 Size: 1501 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3511 3885 4031 387 Size: 1064 138 1922 374 146 1280 Fragments arranged by size: 1922 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2228 2987 3584 4371 4639 1239 Size: 393 524 72 759 597 787 268 1524 Fragments arranged by size: 1524 787 759 597 524 393 268 72 EcoO109I rG'GnC_Cy Cuts at: 2080 2080 Size: 4924 EcoRI G'AATT_C Cuts at: 2233 2611 2233 Size: 378 4546 FspI TGC'GCA Cuts at: 3430 3430 Size: 4924 HaeII r_GCGC'y Cuts at: 1678 4071 1678 Size: 2393 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 3565 3597 3639 Size: 32 135 32 581 32 1380 32 42 Cuts at: 3639 3671 1373 Size: 32 2626 Fragments arranged by size: 2626 1380 581 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2268 2514 669 Size: 988 281 330 246 3079 Fragments arranged by size: 3079 988 330 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4924 HpaI GTT'AAC Cuts at: 2514 2514 Size: 4924 KpnI G_GTAC'C Cuts at: 2115 2249 2115 Size: 134 4790 MfeI C'AATT_G Cuts at: 2523 2523 Size: 4924 MluI A'CGCG_T Cuts at: 4800 4800 Size: 4924 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2099 4038 4222 1305 Size: 794 1939 184 2007 Fragments arranged by size: 2007 1939 794 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3102 Fragments arranged by size: 3102 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2882 3241 3400 518 Size: 648 1716 359 159 2042 Fragments arranged by size: 2042 1716 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2340 3017 4165 4410 1606 Size: 734 677 1148 245 2120 Fragments arranged by size: 2120 1148 734 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4372 NdeI CA'TA_TG Cuts at: 387 387 Size: 4924 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4924 NotI GC'GGCC_GC Cuts at: 2371 2371 Size: 4924 NspI r_CATG'y Cuts at: 1132 1229 2282 3827 1132 Size: 97 1053 1545 2229 Fragments arranged by size: 2229 1545 1053 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4924 PciI A'CATG_T Cuts at: 3823 3823 Size: 4924 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4924 PmeI GTTT'AAAC Cuts at: 2228 2228 Size: 4924 PmlI CAC'GTG Cuts at: 2167 2167 Size: 4924 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2993 3025 4506 4538 2993 Size: 32 1481 32 3379 Fragments arranged by size: 3379 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2080 Size: 4924 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4924 PsiI TTA'TAA Cuts at: 2494 2494 Size: 4924 PstI C_TGCA'G Cuts at: 830 2276 830 Size: 1446 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4924 SalI G'TCGA_C Cuts at: 1936 2266 1936 Size: 330 4594 SbfI CC_TGCA'GG Cuts at: 2276 2276 Size: 4924 ScaI AGT'ACT Cuts at: 3172 4884 3172 Size: 1712 3212 SfcI C'TryA_G Cuts at: 826 1045 2272 3407 4088 4279 826 Size: 219 1227 1135 681 191 1471 Fragments arranged by size: 1471 1227 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4924 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4924 SmaI CCC'GGG Cuts at: 1872 2251 1872 Size: 379 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 3028 3929 4191 820 Size: 197 926 141 944 901 262 1553 Fragments arranged by size: 1553 944 926 901 262 197 141 SnaBI TAC'GTA Cuts at: 493 2715 493 Size: 2222 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4924 SphI G_CATG'C Cuts at: 1132 2282 1132 Size: 1150 3774 SspI AAT'ATT Cuts at: 5 52 2848 5 Size: 47 2796 2081 Fragments arranged by size: 2796 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4924 StyI C'CwwG_G Cuts at: 513 1065 2363 513 Size: 552 1298 3074 Fragments arranged by size: 3074 1298 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2951 2968 3121 3306 3751 325 Size: 1255 1371 17 153 185 445 1498 Fragments arranged by size: 1498 1371 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3170 4882 96 Size: 274 80 33 51 1443 1193 1712 138 Fragments arranged by size: 1712 1443 1193 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4884 4916 4884 Size: 32 4892 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4924 XbaI T'CTAG_A Cuts at: 2260 2260 Size: 4924 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4924 XmaI C'CCGG_G Cuts at: 1870 2249 1870 Size: 379 4545 XmnI GAAnn'nnTTC Cuts at: 3053 3053 Size: 4924 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2732 276 Size: 53 83 186 936 1198 2468 Fragments arranged by size: 2468 1198 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI