(Circular) (Six-base) MAPSORT of: pFN21AB6689.seq Check: 4796 from: 1 to: 5710 pFN21AB6689 5710 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2989 3520 278 Size: 53 83 186 936 1453 531 2468 Fragments arranged by size: 2468 1453 936 531 186 83 53 AccI GT'mk_AC Cuts at: 1937 2256 3053 1937 Size: 319 797 4594 Fragments arranged by size: 4594 797 319 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2456 2958 3773 5157 5425 1632 Size: 824 502 815 1384 268 1917 Fragments arranged by size: 1917 1384 824 815 502 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 2927 3453 5025 2927 Size: 526 1572 3612 Fragments arranged by size: 3612 1572 526 ApaI G_GGCC'C Cuts at: 2013 2013 Size: 5710 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 3019 3245 3397 5506 1493 Size: 1526 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 226 152 AseI AT'TA_AT Cuts at: 160 1032 2829 4264 160 Size: 872 1797 1435 1606 Fragments arranged by size: 1797 1606 1435 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2343 3035 1870 Size: 73 400 692 4545 Fragments arranged by size: 4545 692 400 73 BamHI G'GATC_C Cuts at: 2357 2604 3040 3407 2357 Size: 247 436 367 4660 Fragments arranged by size: 4660 436 367 247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3031 4486 618 Size: 325 217 627 1244 1455 1842 Fragments arranged by size: 1842 1455 1244 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2898 3029 1391 Size: 622 885 131 4072 Fragments arranged by size: 4072 885 622 131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2355 2981 928 Size: 971 456 626 3657 Fragments arranged by size: 3657 971 626 456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2948 2982 3901 3935 1903 Size: 34 1011 34 919 34 3678 Fragments arranged by size: 3678 1011 919 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BfrBI ATG'CAT Cuts at: 2318 2318 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2013 3771 4927 1790 Size: 223 1758 1156 2573 Fragments arranged by size: 2573 1758 1156 223 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2613 2687 3441 Size: 416 53 83 399 24 1196 74 754 Cuts at: 3441 4399 442 Size: 958 1753 Fragments arranged by size: 1753 1196 958 754 416 399 83 74 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2789 2789 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4370 1239 Size: 3131 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2091 3835 4700 4998 2091 Size: 1744 865 298 2803 Fragments arranged by size: 2803 1744 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 882 Size: 617 2874 2219 Fragments arranged by size: 2874 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2986 3517 3899 4534 Size: 53 83 186 936 1453 531 382 635 Cuts at: 4534 275 Size: 1451 Fragments arranged by size: 1453 1451 936 635 531 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2415 1186 Size: 1229 4481 BseYI C'CCAG_C Cuts at: 2729 4913 5409 2729 Size: 2184 496 3030 Fragments arranged by size: 3030 2184 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2014 1163 Size: 573 278 4859 Fragments arranged by size: 4859 573 278 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3771 3856 4927 3771 Size: 85 1071 4554 Fragments arranged by size: 4554 1071 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2898 3029 3771 3856 4927 1391 Size: 399 223 885 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 885 742 399 223 131 85 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2100 3065 844 Size: 1256 965 3489 Fragments arranged by size: 3489 1256 965 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2806 4354 4541 1700 Size: 110 996 1548 187 2869 Fragments arranged by size: 2869 1548 996 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 2626 3770 4782 2626 Size: 1144 1012 3554 Fragments arranged by size: 3554 1144 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2357 2604 3040 3407 3792 3809 5261 5347 Size: 385 247 436 367 385 17 1452 86 Cuts at: 5347 5359 5705 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 436 385 385 367 346 247 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4219 Fragments arranged by size: 4219 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 2100 3226 4020 4040 2100 Size: 1126 794 20 3770 Fragments arranged by size: 3770 1126 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 2079 3014 2079 Size: 935 4775 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675 Size: 54 1425 50 275 18 1327 889 1629 Cuts at: 5675 8 Size: 43 Fragments arranged by size: 1629 1425 1327 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3640 1353 Size: 2287 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2456 2958 3773 4370 5157 5425 1239 Size: 393 824 502 815 597 787 268 1524 Fragments arranged by size: 1524 824 815 787 597 502 393 268 EcoO109I rG'GnC_Cy Cuts at: 2009 2010 2056 2642 2009 Size: 1 46 586 5077 Fragments arranged by size: 5077 586 46 1 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2512 2512 Size: 5710 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 4857 1678 Size: 3179 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 2844 2876 4351 Size: 32 135 32 770 32 470 32 1475 Cuts at: 4351 4383 4425 4457 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1475 770 470 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3668 4027 4186 518 Size: 648 2502 359 159 2042 Fragments arranged by size: 2502 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3126 3803 4951 5196 1606 Size: 1520 677 1148 245 2120 Fragments arranged by size: 2120 1520 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4219 Fragments arranged by size: 4219 939 552 NdeI CA'TA_TG Cuts at: 387 2248 387 Size: 1861 3849 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NsiI A_TGCA'T Cuts at: 2320 2320 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 2683 3068 4613 1132 Size: 97 1454 385 1545 2229 Fragments arranged by size: 2229 1545 1454 385 97 PasI CC'CwG_GG Cuts at: 1348 2478 2577 1348 Size: 1130 99 4481 Fragments arranged by size: 4481 1130 99 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2642 2056 Size: 586 5124 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PspOMI G'GGCC_C Cuts at: 2009 2009 Size: 5710 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2519 2551 2519 Size: 32 5678 PstI C_TGCA'G Cuts at: 830 3062 830 Size: 2232 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 3058 4193 4874 5065 826 Size: 219 2013 1135 681 191 1471 Fragments arranged by size: 2013 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 2345 3037 1872 Size: 473 692 4545 Fragments arranged by size: 4545 692 473 SmlI C'TyrA_G Cuts at: 820 1017 1943 2106 3814 4715 4977 820 Size: 197 926 163 1708 901 262 1553 Fragments arranged by size: 1708 1553 926 901 262 197 163 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 2683 3068 1132 Size: 1551 385 3774 Fragments arranged by size: 3774 1551 385 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 3149 513 Size: 552 939 1145 3074 Fragments arranged by size: 3074 1145 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2146 2682 3737 3754 3907 4092 4537 Size: 1255 566 536 1055 17 153 185 445 Cuts at: 4537 325 Size: 1498 Fragments arranged by size: 1498 1255 1055 566 536 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 2693 1316 Size: 1377 4333 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 2343 3035 1870 Size: 473 692 4545 Fragments arranged by size: 4545 692 473 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2987 3518 276 Size: 53 83 186 936 1453 531 2468 Fragments arranged by size: 2468 1453 936 531 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI