Restriction map for
pFN21AB6689
(Linear) (Six-base) MAPSORT of: KIBB6689.seq Check: 4493 from: 1 to: 1005
KIBB6689.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 0 984 1005
Size: 984 21
AccI GT'mk_AC
Cuts at: 0 251 1005
Size: 251 754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 953 1005
Size: 451 502 52
Fragments arranged by size:
502 451 52
AlwNI CAG_nnn'CTG
Cuts at: 0 922 1005
Size: 922 83
ApaI G_GGCC'C
Cuts at: 0 8 1005
Size: 8 997
AseI AT'TA_AT
Cuts at: 0 824 1005
Size: 824 181
AvaI C'yCGr_G
Cuts at: 0 338 1005
Size: 338 667
BamHI G'GATC_C
Cuts at: 0 352 599 1005
Size: 352 247 406
Fragments arranged by size:
406 352 247
BanII G_rGCy'C
Cuts at: 0 8 893 1005
Size: 8 885 112
Fragments arranged by size:
885 112 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 350 976 1005
Size: 350 626 29
Fragments arranged by size:
626 350 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 943 977 1005
Size: 943 34 28
Fragments arranged by size:
943 34 28
BfrBI ATG'CAT
Cuts at: 0 313 1005
Size: 313 692
Bme1580I G_kGCm'C
Cuts at: 0 8 1005
Size: 8 997
BmrI ACTGGGnnnn_n'
Cuts at: 0 608 682 1005
Size: 608 74 323
Fragments arranged by size:
608 323 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 784 1005
Size: 784 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 1005
Size: 86 919
BsaHI Gr'CG_yC
Cuts at: 0 981 1005
Size: 981 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 410 1005
Size: 410 595
BseYI C'CCAG_C
Cuts at: 0 724 1005
Size: 724 281
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 1005
Size: 9 996
Bsp1286I G_dGCh'C
Cuts at: 0 8 893 1005
Size: 8 885 112
Fragments arranged by size:
885 112 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 95 1005
Size: 95 910
BsrFI r'CCGG_y
Cuts at: 0 801 1005
Size: 801 204
BssSI C'ACGA_G
Cuts at: 0 621 1005
Size: 621 384
BstYI r'GATC_y
Cuts at: 0 352 599 1005
Size: 352 247 406
Fragments arranged by size:
406 352 247
BtsI GCAGTG_nn'
Cuts at: 0 95 1005
Size: 95 910
DraI TTT'AAA
Cuts at: 0 74 1005
Size: 74 931
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 953 1005
Size: 451 502 52
Fragments arranged by size:
502 451 52
EcoO109I rG'GnC_Cy
Cuts at: 0 4 5 51 637 1005
Size: 4 1 46 586 368
Fragments arranged by size:
586 368 46 4 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 507 1005
Size: 507 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 839 871 1005
Size: 337 32 470 32 134
Fragments arranged by size:
470 337 134 32 32
NdeI CA'TA_TG
Cuts at: 0 243 1005
Size: 243 762
NsiI A_TGCA'T
Cuts at: 0 315 1005
Size: 315 690
NspI r_CATG'y
Cuts at: 0 678 1005
Size: 678 327
PasI CC'CwG_GG
Cuts at: 0 473 572 1005
Size: 473 99 433
Fragments arranged by size:
473 433 99
PpuMI rG'GwC_Cy
Cuts at: 0 51 637 1005
Size: 51 586 368
Fragments arranged by size:
586 368 51
PspOMI G'GGCC_C
Cuts at: 0 4 1005
Size: 4 1001
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 514 546 1005
Size: 514 32 459
Fragments arranged by size:
514 459 32
SmaI CCC'GGG
Cuts at: 0 340 1005
Size: 340 665
SmlI C'TyrA_G
Cuts at: 0 101 1005
Size: 101 904
SphI G_CATG'C
Cuts at: 0 678 1005
Size: 678 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 141 677 1005
Size: 141 536 328
Fragments arranged by size:
536 328 141
Tth111I GACn'n_nGTC
Cuts at: 0 688 1005
Size: 688 317
XmaI C'CCGG_G
Cuts at: 0 338 1005
Size: 338 667
ZraI GAC'GTC
Cuts at: 0 982 1005
Size: 982 23
Enzymes that do cut:
AatII AccI AcuI AlwNI ApaI AseI AvaI BamHI
BanII BbsI BcgI BfrBI Bme1580I BmrI BplI BpuEI
BsaHI BseRI BseYI BsgI Bsp1286I BspMI BsrFI BssSI
BstYI BtsI DraI Eco57MI EcoO109I FalI Hin4I NdeI
NsiI NspI PasI PpuMI PspOMI PsrI SmaI SmlI
SphI TaqII Tth111I XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AsiSI AvrII
BaeI BanI BbeI BbvCI BciVI BclI BglI BglII
BlpI BmgBI BmtI BpmI Bpu10I BsaI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI StyI
SwaI TatI TstI XbaI XcmI XhoI XmnI