(Circular) (Six-base) MAPSORT of: pFN21AB6655.seq Check: 3764 from: 1 to: 5974 pFN21AB6655 5974 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2067 3784 278 Size: 53 83 186 936 531 1717 2468 Fragments arranged by size: 2468 1717 936 531 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 3216 4037 5421 5689 1632 Size: 653 931 821 1384 268 1917 Fragments arranged by size: 1917 1384 931 821 653 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 3006 4805 3006 Size: 1799 4175 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 2255 1166 Size: 1089 4885 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2741 2773 3023 3055 2741 Size: 32 250 32 5660 Fragments arranged by size: 5660 250 32 32 AlwNI CAG_nnn'CTG Cuts at: 3717 5289 3717 Size: 1572 4402 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 2689 3283 3509 3661 5770 1493 Size: 1196 594 226 152 2109 1697 Fragments arranged by size: 2109 1697 1196 594 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 3299 1870 Size: 73 1356 4545 Fragments arranged by size: 4545 1356 73 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3293 1391 Size: 1902 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3169 3866 5076 3169 Size: 697 1210 4067 Fragments arranged by size: 4067 1210 697 BclI T'GATC_A Cuts at: 1548 2727 2835 1548 Size: 1179 108 4687 Fragments arranged by size: 4687 1179 108 BfrBI ATG'CAT Cuts at: 2552 2568 2552 Size: 16 5958 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3131 4585 136 Size: 107 122 71 631 605 1459 1454 1525 Fragments arranged by size: 1525 1459 1454 631 605 122 107 71 BglII A'GATC_T Cuts at: 2877 5969 2877 Size: 3092 2882 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 4035 5191 1790 Size: 2245 1156 2573 Fragments arranged by size: 2573 2245 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2069 3705 4663 Size: 416 53 83 399 24 652 1636 958 Cuts at: 4663 442 Size: 1753 Fragments arranged by size: 1753 1636 958 652 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2065 2401 2065 Size: 336 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 3050 3209 3275 4634 1239 Size: 821 990 159 66 1359 2579 Fragments arranged by size: 2579 1359 990 821 159 66 Bpu10I CC'TnA_GC Cuts at: 2103 2103 Size: 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4099 4964 5262 4099 Size: 865 298 4811 Fragments arranged by size: 4811 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2213 2600 3065 3074 4637 882 Size: 617 714 387 465 9 1563 2219 Fragments arranged by size: 2219 1563 714 617 465 387 9 BsaAI yAC'GTr Cuts at: 493 1204 2173 3765 493 Size: 711 969 1592 2702 Fragments arranged by size: 2702 1592 969 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 3781 4163 4798 Size: 53 83 186 936 531 1717 382 635 Cuts at: 4798 275 Size: 1451 Fragments arranged by size: 1717 1451 936 635 531 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3006 4407 4805 5079 5226 5637 3006 Size: 1401 398 274 147 411 3343 Fragments arranged by size: 3343 1401 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2741 2771 3023 3053 1178 Size: 30 165 30 1338 30 252 30 4099 Fragments arranged by size: 4099 1338 252 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5974 BseYI C'CCAG_C Cuts at: 2889 5177 5673 2889 Size: 2288 496 3190 Fragments arranged by size: 3190 2288 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5401 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4035 4120 5191 4035 Size: 85 1071 4818 Fragments arranged by size: 4818 1071 85 BsmI GAATG_Cn' Cuts at: 2552 3485 3578 2552 Size: 933 93 4948 Fragments arranged by size: 4948 933 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2428 2428 Size: 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3293 4035 4120 5191 1391 Size: 399 1503 742 85 1071 2174 Fragments arranged by size: 2174 1503 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 3329 844 Size: 1286 1199 3489 Fragments arranged by size: 3489 1286 1199 BsrBI CCG'CTC Cuts at: 1394 1823 3861 1394 Size: 429 2038 3507 Fragments arranged by size: 3507 2038 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2200 4469 4643 65 Size: 1183 952 2269 174 1396 Fragments arranged by size: 2269 1396 1183 952 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3006 4618 4805 1700 Size: 110 1196 1612 187 2869 Fragments arranged by size: 2869 1612 1196 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 3002 1184 Size: 1818 4156 BstXI CCAn_nnnn'nTGG Cuts at: 2257 3112 2257 Size: 855 5119 BstYI r'GATC_y Cuts at: 1972 2877 2991 3304 3671 4056 4073 5525 5611 Size: 905 114 313 367 385 17 1452 86 Cuts at: 5611 5623 5969 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 905 385 367 346 313 114 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2684 2684 Size: 5974 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 513 Size: 552 939 48 4435 Fragments arranged by size: 4435 939 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 2637 3490 4284 4304 2637 Size: 853 794 20 4307 Fragments arranged by size: 4307 853 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DraIII CAC_nnn'GTG Cuts at: 2701 2701 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2079 3114 Size: 54 1425 50 275 18 177 72 1035 Cuts at: 3114 3421 4310 5939 8 Size: 307 889 1629 43 Fragments arranged by size: 1629 1425 1035 889 307 275 177 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2591 3192 3213 3904 1353 Size: 1238 601 21 691 3423 Fragments arranged by size: 3423 1238 691 601 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2285 3050 3209 3216 3275 4037 Size: 393 428 225 765 159 7 59 762 Cuts at: 4037 4634 5421 5689 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 765 762 597 428 393 268 225 159 59 7 EcoO109I rG'GnC_Cy Cuts at: 2380 2680 2380 Size: 300 5674 EcoRI G'AATT_C Cuts at: 2689 3283 3661 2689 Size: 594 378 5002 Fragments arranged by size: 5002 594 378 EcoRV GAT'ATC Cuts at: 2242 2782 2242 Size: 540 5434 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 5121 1678 Size: 3443 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3248 3280 4615 4647 4689 Size: 32 135 32 1676 32 1335 32 42 Cuts at: 4689 4721 1373 Size: 32 2626 Fragments arranged by size: 2626 1676 1335 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3052 3318 3564 669 Size: 988 281 1114 266 246 3079 Fragments arranged by size: 3079 1114 988 281 266 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2081 10 Size: 54 1425 343 177 72 3903 Fragments arranged by size: 3903 1425 343 177 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2951 3932 4291 4450 518 Size: 648 1089 696 981 359 159 2042 Fragments arranged by size: 2042 1089 981 696 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2833 2893 3390 4067 5215 5460 1606 Size: 1227 60 497 677 1148 245 2120 Fragments arranged by size: 2120 1227 1148 677 497 245 60 NcoI C'CATG_G Cuts at: 513 1065 2004 2052 513 Size: 552 939 48 4435 Fragments arranged by size: 4435 939 552 48 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NsiI A_TGCA'T Cuts at: 2554 2570 2554 Size: 16 5958 NspI r_CATG'y Cuts at: 1132 1229 2572 2961 3182 3332 4877 1132 Size: 97 1343 389 221 150 1545 2229 Fragments arranged by size: 2229 1545 1343 389 221 150 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 2701 1108 Size: 1593 4381 PfoI T'CCnGG_A Cuts at: 2037 2387 2037 Size: 350 5624 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2741 2773 3023 3055 4043 4075 5556 5588 2741 Size: 32 250 32 988 32 1481 32 3127 Fragments arranged by size: 3127 1481 988 250 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2380 2680 2380 Size: 300 5674 PshAI GACnn'nnGTC Cuts at: 1613 2033 2612 1613 Size: 420 579 4975 Fragments arranged by size: 4975 579 420 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PstI C_TGCA'G Cuts at: 830 3326 830 Size: 2496 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2893 2893 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SapI GCTCTTCn'nnn_ Cuts at: 3213 3213 Size: 5974 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 3322 4457 5138 5329 826 Size: 219 2277 1135 681 191 1471 Fragments arranged by size: 2277 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4078 4979 5241 820 Size: 197 926 2135 901 262 1553 Fragments arranged by size: 2135 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 3413 513 Size: 552 939 48 1361 3074 Fragments arranged by size: 3074 1361 939 552 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2252 2639 4001 4018 4171 4356 4801 Size: 1255 672 387 1362 17 153 185 445 Cuts at: 4801 325 Size: 1498 Fragments arranged by size: 1498 1362 1255 672 445 387 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4220 5932 96 Size: 274 80 33 51 1443 2243 1712 138 Fragments arranged by size: 2243 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2252 2284 2383 2415 5934 5966 2252 Size: 32 99 32 3519 32 2260 Fragments arranged by size: 3519 2260 99 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2905 2905 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2065 3782 276 Size: 53 83 186 936 531 1717 2468 Fragments arranged by size: 2468 1717 936 531 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI