(Circular) (Six-base) MAPSORT of: pFN21AB6572.seq Check: 7083 from: 1 to: 5905 pFN21AB6572 5905 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2265 3248 1937 Size: 328 983 4594 Fragments arranged by size: 4594 983 328 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 2565 2780 3968 5352 5620 1632 Size: 557 376 215 1188 1384 268 1917 Fragments arranged by size: 1917 1384 1188 557 376 268 215 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 3081 4804 5781 1203 Size: 1878 1723 977 1327 Fragments arranged by size: 1878 1723 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 2246 2396 1166 Size: 1080 150 4675 Fragments arranged by size: 4675 1080 150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2180 1184 Size: 996 4909 AlwNI CAG_nnn'CTG Cuts at: 2778 3648 5220 2778 Size: 870 1572 3463 Fragments arranged by size: 3463 1572 870 ApaI G_GGCC'C Cuts at: 2182 2946 2182 Size: 764 5141 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 2293 2854 3214 3440 3592 5701 1493 Size: 800 561 360 226 152 2109 1697 Fragments arranged by size: 2109 1697 800 561 360 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 2955 3230 1870 Size: 73 1012 275 4545 Fragments arranged by size: 4545 1012 275 73 BamHI G'GATC_C Cuts at: 2670 3235 3602 2670 Size: 565 367 4973 Fragments arranged by size: 4973 565 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 3226 4681 618 Size: 325 217 627 282 1157 1455 1842 Fragments arranged by size: 1842 1455 1157 627 325 282 217 BanII G_rGCy'C Cuts at: 1391 2182 2441 2946 3224 1391 Size: 791 259 505 278 4072 Fragments arranged by size: 4072 791 505 278 259 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2128 2852 928 Size: 971 229 724 3981 Fragments arranged by size: 3981 971 724 229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 2463 1548 Size: 915 4990 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 4516 136 Size: 107 122 71 631 605 406 2438 1525 Fragments arranged by size: 2438 1525 631 605 406 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 2885 3322 2885 Size: 437 5468 Bme1580I G_kGCm'C Cuts at: 1790 2182 2946 3966 5122 1790 Size: 392 764 1020 1156 2573 Fragments arranged by size: 2573 1156 1020 764 392 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3636 4594 442 Size: 416 53 83 399 24 2219 958 1753 Fragments arranged by size: 2219 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2774 4565 1239 Size: 1052 483 1791 2579 Fragments arranged by size: 2579 1791 1052 483 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3173 4030 4895 5193 3173 Size: 857 865 298 3885 Fragments arranged by size: 3885 865 857 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4568 882 Size: 617 3069 2219 Fragments arranged by size: 3069 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3082 3124 3696 493 Size: 711 1878 42 572 2702 Fragments arranged by size: 2702 1878 711 572 42 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3712 4094 4729 275 Size: 53 83 186 936 2179 382 635 1451 Fragments arranged by size: 2179 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3174 4338 4736 5010 5157 5568 3174 Size: 1164 398 274 147 411 3511 Fragments arranged by size: 3511 1164 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2601 2631 2719 2749 1178 Size: 30 165 30 1198 30 88 30 4334 Fragments arranged by size: 4334 1198 165 88 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2835 2838 2855 1186 Size: 1649 3 17 4236 Fragments arranged by size: 4236 1649 17 3 BseYI C'CCAG_C Cuts at: 2938 3115 5108 5604 2938 Size: 177 1993 496 3239 Fragments arranged by size: 3239 1993 496 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5332 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2472 3355 4116 5144 1540 Size: 275 186 471 883 761 1028 2301 Fragments arranged by size: 2301 1028 883 761 471 275 186 BsiHKAI G_wGCw'C Cuts at: 2441 3966 4051 5122 2441 Size: 1525 85 1071 3224 Fragments arranged by size: 3224 1525 1071 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 BsmBI CGTCTCn'nnnn_ Cuts at: 2653 2653 Size: 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2182 2441 2946 3224 3966 4051 5122 Size: 399 392 259 505 278 742 85 1071 Cuts at: 5122 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 505 399 392 278 259 85 BspEI T'CCGG_A Cuts at: 3174 5568 3174 Size: 2394 3511 BspHI T'CATG_A Cuts at: 2337 2460 3794 5524 2337 Size: 123 1334 1730 2718 Fragments arranged by size: 2718 1730 1334 123 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2607 3260 844 Size: 1763 653 3489 Fragments arranged by size: 3489 1763 653 BsrBI CCG'CTC Cuts at: 1394 1823 2176 3792 1394 Size: 429 353 1616 3507 Fragments arranged by size: 3507 1616 429 353 BsrDI GCAATG_nn' Cuts at: 65 1248 2149 2917 4400 4574 65 Size: 1183 901 768 1483 174 1396 Fragments arranged by size: 1483 1396 1183 901 768 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4549 4736 1700 Size: 110 2739 187 2869 Fragments arranged by size: 2869 2739 187 110 BsrGI T'GTAC_A Cuts at: 96 2345 96 Size: 2249 3656 BssHII G'CGCG_C Cuts at: 1925 2115 1925 Size: 190 5715 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 2899 4709 2899 Size: 1810 4095 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstXI CCAn_nnnn'nTGG Cuts at: 2027 2027 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2670 3192 3235 3602 3987 4004 5456 5542 Size: 698 522 43 367 385 17 1452 86 Cuts at: 5542 5554 5900 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 698 522 385 367 346 86 43 17 12 BstZ17I GTA'TAC Cuts at: 2266 2266 Size: 5905 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4414 Fragments arranged by size: 4414 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 2391 2403 2787 3064 3421 4215 4235 2391 Size: 12 384 277 357 794 20 4061 Fragments arranged by size: 4061 794 384 357 277 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2540 2688 4912 809 Size: 507 1224 148 2224 1802 Fragments arranged by size: 2224 1802 1224 507 148 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2127 2238 Size: 54 1425 50 275 18 199 98 111 Cuts at: 2238 2469 2997 3352 4241 5870 8 Size: 231 528 355 889 1629 43 Fragments arranged by size: 1629 1425 889 528 355 275 231 199 111 98 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2469 3352 1537 Size: 275 657 883 4090 Fragments arranged by size: 4090 883 657 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2511 2584 3835 1353 Size: 1158 73 1251 3423 Fragments arranged by size: 3423 1251 1158 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4492 4866 5012 387 Size: 1064 138 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2439 2439 Size: 5905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2291 2565 2774 2780 3968 4565 Size: 393 557 102 274 209 6 1188 597 Cuts at: 4565 5352 5620 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1188 787 597 557 393 274 268 209 102 6 EcoO109I rG'GnC_Cy Cuts at: 2179 2533 2942 2943 3012 2179 Size: 354 409 1 69 5072 Fragments arranged by size: 5072 409 354 69 1 EcoRI G'AATT_C Cuts at: 2854 3214 3592 2854 Size: 360 378 5167 Fragments arranged by size: 5167 378 360 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2905 2905 Size: 5905 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2879 3135 5052 1678 Size: 1201 256 1917 2531 Fragments arranged by size: 2531 1917 1201 256 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2428 2460 2601 2633 2662 Size: 32 135 32 856 32 141 32 29 Cuts at: 2662 2694 4546 4578 4620 4652 1373 Size: 32 1852 32 42 32 2626 Fragments arranged by size: 2626 1852 856 141 135 42 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 3249 3495 669 Size: 988 281 1311 246 3079 Fragments arranged by size: 3079 1311 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2580 5019 5203 1305 Size: 1275 2439 184 2007 Fragments arranged by size: 2439 2007 1275 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2031 2129 2240 2999 10 Size: 54 1425 343 199 98 111 759 2916 Fragments arranged by size: 2916 1425 759 343 199 111 98 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2246 2396 3863 4222 4381 518 Size: 648 996 84 150 1467 359 159 2042 Fragments arranged by size: 2042 1467 996 648 359 159 150 84 MspA1I CmG'CkG Cuts at: 1606 2023 3321 3998 5146 5391 1606 Size: 417 1298 677 1148 245 2120 Fragments arranged by size: 2120 1298 1148 677 417 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4414 Fragments arranged by size: 4414 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 2093 3078 3263 4808 1132 Size: 97 864 985 185 1545 2229 Fragments arranged by size: 2229 1545 985 864 185 97 PasI CC'CwG_GG Cuts at: 1348 2256 1348 Size: 908 4997 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 2995 3111 1108 Size: 1887 116 3902 Fragments arranged by size: 3902 1887 116 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PmlI CAC'GTG Cuts at: 3082 3082 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2533 2533 Size: 5905 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2178 2942 2178 Size: 764 5141 PstI C_TGCA'G Cuts at: 830 2187 2398 3257 830 Size: 1357 211 859 3478 Fragments arranged by size: 3478 1357 859 211 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 PvuII CAG'CTG Cuts at: 2023 2023 Size: 5905 SacI G_AGCT'C Cuts at: 2441 2441 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SanDI GG'GwC_CC Cuts at: 2533 2533 Size: 5905 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2183 2394 2770 3253 4388 5069 5260 Size: 219 1138 211 376 483 1135 681 191 Cuts at: 5260 826 Size: 1471 Fragments arranged by size: 1471 1138 1135 681 483 376 219 211 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3188 4009 4910 5172 820 Size: 197 926 1245 821 901 262 1553 Fragments arranged by size: 1553 1245 926 901 821 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 2710 1517 Size: 1193 4712 StyI C'CwwG_G Cuts at: 513 1065 2004 2244 2793 3344 513 Size: 552 939 240 549 551 3074 Fragments arranged by size: 3074 939 552 551 549 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2248 2786 3932 3949 4102 4287 4732 Size: 1255 668 538 1146 17 153 185 445 Cuts at: 4732 325 Size: 1498 Fragments arranged by size: 1498 1255 1146 668 538 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2345 4151 5863 Size: 274 80 33 51 1443 368 1806 1712 Cuts at: 5863 96 Size: 138 Fragments arranged by size: 1806 1712 1443 368 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5865 5897 5865 Size: 32 5873 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 3112 2638 Size: 474 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 3182 4034 3182 Size: 852 5053 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI