Restriction map for
pFN21AB6423
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstF5I
BstPI BstUI BstYI BstZ17I CfoI
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FbaI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NarI NciI
NgoMIV NotI NruI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AlwI 397 GGATC GGATC(4/5)
AocI 853 CCTGAGG CC^T[ACGT]AGG
AspHI 316 GAGCTC G[AT]GC[AT]^C
AvaII 481 GGTCC G^G[AT]CC
BbsI 346 GTCTTC GAAGAC(2/6)
BciVI 303 GTATCC GTATCC(6/5)
BlpI 764 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 764 GCTTAGC GC^T[ACGT]AGC
BpuAI 346 GTCTTC GAAGAC(2/6)
BsiHKAI 316 GAGCTC G[AT]GC[AT]^C
BsiYI 472 CCCTCAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 472 CCCTCAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 632 GAATGC GAATGC(1/-1)
BsrDI 368 CATTGC GCAATG(2/0)
BsrFI 122 ACCGGC [GA]^CCGG[CT]
BsrI 736 ACTGG ACTGG(1/-1)
BstBI 439 TTCGAA TT^CGAA
BstXI 447 CCACAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 853 CCTGAGG CC^T[ACGT]AGG
CelII 764 GCTTAGC GC^T[ACGT]AGC
Cfr10I 122 ACCGGC [GA]^CCGG[CT]
DraIII 523 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 853 CCTGAGG CC^T[ACGT]AGG
EcoT14I 453 CCATGG C^C[AT][AT]GG
EcoT22I 184 ATGCAT ATGCA^T
FauI 759 GCGGG CCCGC(4/6)
HapII 123 CCGG C^CGG
HpaII 123 CCGG C^CGG
MspI 123 CCGG C^CGG
NcoI 453 CCATGG C^CATGG
NsiI 184 ATGCAT ATGCA^T
NspV 439 TTCGAA TT^CGAA
PpuMI 480 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 553 CTGCAG CTGCA^G
PvuII 794 CAGCTG CAG^CTG
SacI 316 GAGCTC GAGCT^C
SfcI 553 CTGCAG C^T[GA][CT]AG
SfuI 439 TTCGAA TT^CGAA
StyI 453 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 652 GGTACC G^GTACC
799 GGTACC
AcsI 267 AAATTC [GA]^AATT[CT]
827 AAATTC
AfaI 653 GTAC GT^AC
800 GTAC
ApoI 267 AAATTC [GA]^AATT[CT]
827 AAATTC
Asp700I 266 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
826 GAAATTCTTC
Asp718I 652 GGTACC G^GTACC
799 GGTACC
BanI 652 GGTACC G^G[CT][GA]CC
799 GGTACC
DpnII 251 GATC ^GATC
398 GATC
DraII 480 GGGTCCC [GA]G^G[ACGT]CC[CT]
857 AGGCCCC
DsaI 453 CCATGG C^C[GA][CT]GG
835 CCACGG
EcoO109I 480 GGGTCCC [GA]G^G[ACGT]CC[CT]
857 AGGCCCC
KpnI 652 GGTACC GGTAC^C
799 GGTACC
MboI 251 GATC ^GATC
398 GATC
MspA1I 794 CAGCTG C[AC]G^C[GT]G
887 CCGCTG
NdeI 187 CATATG CA^TATG
295 CATATG
NdeII 251 GATC ^GATC
398 GATC
NheI 540 GCTAGC G^CTAGC
843 GCTAGC
RsaI 653 GTAC GT^AC
800 GTAC
Sau3AI 251 GATC ^GATC
398 GATC
SfaNI 103 GATGC GCATC(5/9)
280 GATGC
TaqI 400 TCGA T^CGA
440 TCGA
TthHB8I 400 TCGA T^CGA
440 TCGA
XmnI 266 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
826 GAAATTCTTC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BanII BbvI BfaI
BglI BmyI BpmI BsaJI BsgI
BsmFI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboII MnlI MslI MvaI MwoI
NlaIII NlaIV PleI Sau96I ScrFI
TfiI TseI Tsp45I Tsp509I TspRI