(Circular) (Six-base) MAPSORT of: pFN21AB6423.seq Check: 5295 from: 1 to: 5632 pFN21AB6423 5632 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 1937 Size: 1038 4594 Acc65I G'GTAC_C Cuts at: 2657 2804 2953 2657 Size: 147 149 5336 Fragments arranged by size: 5336 149 147 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2223 2775 3695 5079 5347 1632 Size: 591 552 920 1384 268 1917 Fragments arranged by size: 1917 1384 920 591 552 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5632 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2214 2395 1184 Size: 1030 181 4421 Fragments arranged by size: 4421 1030 181 AlwNI CAG_nnn'CTG Cuts at: 3375 4947 3375 Size: 1572 4060 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2272 2832 2941 3167 3319 5428 1493 Size: 779 560 109 226 152 2109 1697 Fragments arranged by size: 2109 1697 779 560 226 152 109 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2957 1870 Size: 73 1014 4545 Fragments arranged by size: 4545 1014 73 BamHI G'GATC_C Cuts at: 2962 3329 2962 Size: 367 5265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2657 2804 2953 4408 618 Size: 325 217 627 870 147 149 1455 1842 Fragments arranged by size: 1842 1455 870 627 325 217 149 147 BanII G_rGCy'C Cuts at: 1391 2325 2437 2500 2951 1391 Size: 934 112 63 451 4072 Fragments arranged by size: 4072 934 451 112 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2344 928 Size: 971 445 4216 Fragments arranged by size: 4216 971 445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3823 3857 1903 Size: 34 1886 34 3678 Fragments arranged by size: 3678 1886 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2319 3524 4734 2319 Size: 1205 1210 3217 Fragments arranged by size: 3217 1210 1205 BclI T'GATC_A Cuts at: 1548 1548 Size: 5632 BfrBI ATG'CAT Cuts at: 2191 2191 Size: 5632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2051 2909 2927 Size: 107 122 71 631 605 379 858 18 Cuts at: 2927 4243 136 Size: 1316 1525 Fragments arranged by size: 1525 1316 858 631 605 379 122 107 71 18 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 2770 3049 2770 Size: 279 5353 Bme1580I G_kGCm'C Cuts at: 1790 3693 4849 1790 Size: 1903 1156 2573 Fragments arranged by size: 2573 1903 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3363 4321 442 Size: 416 53 83 399 24 1946 958 1753 Fragments arranged by size: 1946 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2549 2852 1056 Size: 1493 303 3836 Fragments arranged by size: 3836 1493 303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2418 2763 4292 1239 Size: 992 187 345 1529 2579 Fragments arranged by size: 2579 1529 992 345 187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3757 4622 4920 3757 Size: 865 298 4469 Fragments arranged by size: 4469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3439 3821 4456 275 Size: 53 83 186 936 1906 382 635 1451 Fragments arranged by size: 1906 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5407 Fragments arranged by size: 5407 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5632 BseYI C'CCAG_C Cuts at: 4835 5331 4835 Size: 496 5136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2267 2432 2603 1163 Size: 573 531 165 171 4192 Fragments arranged by size: 4192 573 531 171 165 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 3693 3778 4849 2325 Size: 1368 85 1071 3108 Fragments arranged by size: 3108 1368 1071 85 BsmI GAATG_Cn' Cuts at: 2643 3143 3236 2643 Size: 500 93 5039 Fragments arranged by size: 5039 500 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2325 2437 2500 2951 3693 3778 4849 Size: 399 535 112 63 451 742 85 1071 Cuts at: 4849 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 535 451 399 112 85 63 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 3521 5251 3521 Size: 1730 3902 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2987 844 Size: 2143 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2372 4127 4301 65 Size: 1183 1124 1755 174 1396 Fragments arranged by size: 1755 1396 1183 1124 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2127 4276 4463 1700 Size: 110 317 2149 187 2869 Fragments arranged by size: 2869 2149 317 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 2445 4436 2445 Size: 1991 3641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstXI CCAn_nnnn'nTGG Cuts at: 2459 2459 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2962 3329 3714 3731 5183 5269 5281 5627 Size: 990 367 385 17 1452 86 12 346 Cuts at: 5627 1972 Size: 1977 Fragments arranged by size: 1977 1452 990 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2859 2859 Size: 5632 BtgI C'CryG_G Cuts at: 513 1065 2004 2458 2840 513 Size: 552 939 454 382 3305 Fragments arranged by size: 3305 939 552 454 382 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 2384 2682 3148 3942 3962 2384 Size: 298 466 794 20 4054 Fragments arranged by size: 4054 794 466 298 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DraIII CAC_nnn'GTG Cuts at: 2533 2533 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3079 3968 5597 Size: 54 1425 50 275 18 1249 889 1629 Cuts at: 5597 8 Size: 43 Fragments arranged by size: 1629 1425 1249 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3562 1353 Size: 2209 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2323 2323 Size: 5632 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2231 2418 2763 2775 3695 4292 Size: 393 591 8 187 345 12 920 597 Cuts at: 4292 5079 5347 1239 Size: 787 268 1524 Fragments arranged by size: 1524 920 787 597 591 393 345 268 187 12 8 EcoO109I rG'GnC_Cy Cuts at: 2486 2863 2486 Size: 377 5255 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 4779 1678 Size: 3101 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2310 2342 2487 2519 4273 Size: 32 135 32 738 32 145 32 1754 Cuts at: 4273 4305 4347 4379 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1754 738 145 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2976 3222 669 Size: 988 281 1038 246 3079 Fragments arranged by size: 3079 1038 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5632 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KpnI G_GTAC'C Cuts at: 2661 2808 2957 2661 Size: 147 149 5336 Fragments arranged by size: 5336 149 147 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4746 4930 1305 Size: 3441 184 2007 Fragments arranged by size: 3441 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3810 Fragments arranged by size: 3810 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2377 2457 3590 3949 4108 518 Size: 648 1026 185 80 1133 359 159 2042 Fragments arranged by size: 2042 1133 1026 648 359 185 159 80 MspA1I CmG'CkG Cuts at: 1606 2801 2894 3048 3725 4873 5118 1606 Size: 1195 93 154 677 1148 245 2120 Fragments arranged by size: 2120 1195 1148 677 245 154 93 NcoI C'CATG_G Cuts at: 513 1065 2004 2458 513 Size: 552 939 454 3687 Fragments arranged by size: 3687 939 552 454 NdeI CA'TA_TG Cuts at: 387 2193 2301 387 Size: 1806 108 3718 Fragments arranged by size: 3718 1806 108 NheI G'CTAG_C Cuts at: 1052 2545 2848 1052 Size: 1493 303 3836 Fragments arranged by size: 3836 1493 303 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NsiI A_TGCA'T Cuts at: 2193 2193 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2990 4535 1132 Size: 97 1761 1545 2229 Fragments arranged by size: 2229 1761 1545 97 PasI CC'CwG_GG Cuts at: 1348 2335 2713 1348 Size: 987 378 4267 Fragments arranged by size: 4267 987 378 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2486 2486 Size: 5632 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PstI C_TGCA'G Cuts at: 830 2562 2984 830 Size: 1732 422 3478 Fragments arranged by size: 3478 1732 422 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 PvuII CAG'CTG Cuts at: 2801 2801 Size: 5632 SacI G_AGCT'C Cuts at: 2325 2325 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SanDI GG'GwC_CC Cuts at: 2486 2486 Size: 5632 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SfcI C'TryA_G Cuts at: 826 1045 2558 2980 4115 4796 4987 826 Size: 219 1513 422 1135 681 191 1471 Fragments arranged by size: 1513 1471 1135 681 422 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3736 4637 4899 820 Size: 197 926 1793 901 262 1553 Fragments arranged by size: 1793 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5632 StyI C'CwwG_G Cuts at: 513 1065 2004 2458 3071 513 Size: 552 939 454 613 3074 Fragments arranged by size: 3074 939 613 552 454 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3659 3676 3829 4014 4459 325 Size: 1255 2079 17 153 185 445 1498 Fragments arranged by size: 2079 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3878 5590 96 Size: 274 80 33 51 1443 1901 1712 138 Fragments arranged by size: 1901 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2394 2426 5592 5624 2394 Size: 32 3166 32 2402 Fragments arranged by size: 3166 2402 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 2275 2835 3761 2275 Size: 560 926 4146 Fragments arranged by size: 4146 926 560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI