(Circular) (Six-base) MAPSORT of: pFN21AE0606.seq Check: 7626 from: 1 to: 6064 pFN21AE0606 6064 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2734 3064 2734 Size: 330 5734 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2694 3407 1937 Size: 757 713 4594 Fragments arranged by size: 4594 757 713 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2410 4127 5511 5779 1632 Size: 778 1717 1384 268 1917 Fragments arranged by size: 1917 1717 1384 778 268 AfeI AGC'GCT Cuts at: 2402 2402 Size: 6064 AflII C'TTAA_G Cuts at: 820 1017 3232 820 Size: 197 2215 3652 Fragments arranged by size: 3652 2215 197 AflIII A'CryG_T Cuts at: 1203 4963 5940 1203 Size: 3760 977 1327 Fragments arranged by size: 3760 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 4793 4793 Size: 6064 AleI CACnn'nnGTG Cuts at: 1166 2171 1166 Size: 1005 5059 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 2034 2176 2652 3079 3807 5379 2034 Size: 142 476 427 728 1572 2719 Fragments arranged by size: 2719 1572 728 476 427 142 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 3373 3599 3751 5860 1493 Size: 1880 226 152 2109 1697 Fragments arranged by size: 2109 1880 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2066 2609 3287 3389 1870 Size: 73 80 43 543 678 102 4545 Fragments arranged by size: 4545 678 543 102 80 73 43 BamHI G'GATC_C Cuts at: 3394 3761 3394 Size: 367 5697 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2681 3195 3385 4840 618 Size: 325 217 627 894 514 190 1455 1842 Fragments arranged by size: 1842 1455 894 627 514 325 217 190 BanII G_rGCy'C Cuts at: 1391 2771 3261 3303 3383 1391 Size: 1380 490 42 80 4072 Fragments arranged by size: 4072 1380 490 80 42 BbeI G_GCGC'C Cuts at: 2685 3199 2685 Size: 514 5550 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5093 BbvCI CC'TCA_GC Cuts at: 2801 3008 2801 Size: 207 5857 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3038 3072 4255 4289 1903 Size: 34 1101 34 1183 34 3678 Fragments arranged by size: 3678 1183 1101 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2183 3956 5166 2183 Size: 1773 1210 3081 Fragments arranged by size: 3081 1773 1210 BclI T'GATC_A Cuts at: 1548 2050 2118 1548 Size: 502 68 5494 Fragments arranged by size: 5494 502 68 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2025 2501 2766 Size: 107 122 71 631 605 353 476 265 Cuts at: 2766 4675 136 Size: 1909 1525 Fragments arranged by size: 1909 1525 631 605 476 353 265 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 3481 3481 Size: 6064 Bme1580I G_kGCm'C Cuts at: 1790 2556 2786 4125 5281 1790 Size: 766 230 1339 1156 2573 Fragments arranged by size: 2573 1339 1156 766 230 BmgBI CAC'GTC Cuts at: 1318 2307 1318 Size: 989 5075 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3256 3268 3795 Size: 416 53 83 399 24 1839 12 527 Cuts at: 3795 4753 442 Size: 958 1753 Fragments arranged by size: 1839 1753 958 527 416 399 83 53 24 12 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3322 3322 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2569 2629 4724 1239 Size: 1330 60 2095 2579 Fragments arranged by size: 2579 2095 1330 60 Bpu10I CC'TnA_GC Cuts at: 2801 3008 2801 Size: 207 5857 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4189 5054 5352 4189 Size: 865 298 4901 Fragments arranged by size: 4901 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2573 2606 4727 882 Size: 617 1074 33 2121 2219 Fragments arranged by size: 2219 2121 1074 617 33 BsaAI yAC'GTr Cuts at: 493 1204 2380 3855 493 Size: 711 1176 1475 2702 Fragments arranged by size: 2702 1475 1176 711 BsaBI GATnn'nnATC Cuts at: 2093 6058 2093 Size: 3965 2099 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2682 3196 3871 4253 Size: 53 83 186 936 1149 514 675 382 Cuts at: 4253 4888 275 Size: 635 1451 Fragments arranged by size: 1451 1149 936 675 635 514 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4497 4895 5169 5316 5727 4497 Size: 398 274 147 411 4834 Fragments arranged by size: 4834 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2004 2034 2776 2806 1178 Size: 30 165 30 601 30 742 30 4436 Fragments arranged by size: 4436 742 601 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2969 1186 Size: 1783 4281 BseYI C'CCAG_C Cuts at: 2015 2555 5267 5763 2015 Size: 540 2712 496 2316 Fragments arranged by size: 2712 2316 540 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2360 3092 3188 1163 Size: 573 582 42 732 96 4039 Fragments arranged by size: 4039 732 582 573 96 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2751 4125 4210 5281 2751 Size: 1374 85 1071 3534 Fragments arranged by size: 3534 1374 1071 85 BsmI GAATG_Cn' Cuts at: 3575 3668 3575 Size: 93 5971 BsmBI CGTCTCn'nnnn_ Cuts at: 2987 2987 Size: 6064 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2556 2751 2771 2786 3261 3303 3383 Size: 399 766 195 20 15 475 42 80 Cuts at: 3383 4125 4210 5281 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 766 742 475 399 195 85 80 42 20 15 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 2751 3953 5683 2751 Size: 1202 1730 3132 Fragments arranged by size: 3132 1730 1202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2734 3064 3419 844 Size: 1890 330 355 3489 Fragments arranged by size: 3489 1890 355 330 BsrBI CCG'CTC Cuts at: 1394 1823 2583 3028 3951 1394 Size: 429 760 445 923 3507 Fragments arranged by size: 3507 923 760 445 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4559 4733 65 Size: 1183 3311 174 1396 Fragments arranged by size: 3311 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4708 4895 1700 Size: 110 2898 187 2869 Fragments arranged by size: 2898 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3033 96 Size: 2937 3127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 2600 2699 3113 4124 5136 2600 Size: 99 414 1011 1012 3528 Fragments arranged by size: 3528 1012 1011 414 99 BstAPI GCAn_nnn'nTGC Cuts at: 794 2034 794 Size: 1240 4824 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 2164 2485 1184 Size: 980 321 4763 Fragments arranged by size: 4763 980 321 BstYI r'GATC_y Cuts at: 1972 3394 3761 4146 4163 5615 5701 5713 6059 Size: 1422 367 385 17 1452 86 12 346 Cuts at: 6059 1972 Size: 1977 Fragments arranged by size: 1977 1452 1422 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2858 2858 Size: 6064 BtgI C'CryG_G Cuts at: 513 1065 2004 2268 2523 513 Size: 552 939 264 255 4054 Fragments arranged by size: 4054 939 552 264 255 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2392 487 Size: 683 56 1166 4159 Fragments arranged by size: 4159 1166 683 56 BtsI GCAGTG_nn' Cuts at: 2035 2504 3580 4374 4394 2035 Size: 469 1076 794 20 3705 Fragments arranged by size: 3705 1076 794 469 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DraIII CAC_nnn'GTG Cuts at: 2221 2221 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2775 2880 Size: 54 1425 50 275 18 249 696 105 Cuts at: 2880 2925 3511 4400 6029 8 Size: 45 586 889 1629 43 Fragments arranged by size: 1629 1425 889 696 586 275 249 105 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2351 2814 3188 3994 1353 Size: 998 463 374 806 3423 Fragments arranged by size: 3423 998 806 463 374 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2482 4651 5025 5171 387 Size: 1064 138 893 2169 374 146 1280 Fragments arranged by size: 2169 1280 1064 893 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2410 2569 2629 4127 4724 5511 5779 Size: 393 778 159 60 1498 597 787 268 Cuts at: 5779 1239 Size: 1524 Fragments arranged by size: 1524 1498 787 778 597 393 268 159 60 EcoNI CCTnn'n_nnAGG Cuts at: 2348 3208 2348 Size: 860 5204 EcoO109I rG'GnC_Cy Cuts at: 2447 2952 2447 Size: 505 5559 EcoRI G'AATT_C Cuts at: 3373 3751 3373 Size: 378 5686 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3247 3247 Size: 6064 FspI TGC'GCA Cuts at: 3138 4570 3138 Size: 1432 4632 FspAI rTGC'GCAy Cuts at: 3138 3138 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 2154 2404 2685 3124 3159 3175 3199 5211 Size: 476 250 281 439 35 16 24 2012 Cuts at: 5211 1678 Size: 2531 Fragments arranged by size: 2531 2012 476 439 281 250 35 24 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3248 3280 4705 4737 4779 Size: 32 135 32 1676 32 1425 32 42 Cuts at: 4779 4811 1373 Size: 32 2626 Fragments arranged by size: 2626 1676 1425 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3408 3654 669 Size: 988 281 1470 246 3079 Fragments arranged by size: 3079 1470 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 3654 3654 Size: 6064 KasI G'GCGC_C Cuts at: 2681 3195 2681 Size: 514 5550 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5178 5362 1305 Size: 3873 184 2007 Fragments arranged by size: 3873 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 10 Size: 54 1425 343 249 3993 Fragments arranged by size: 3993 1425 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2058 2126 2171 2246 2261 2267 4022 Size: 648 892 68 45 75 15 6 1755 Cuts at: 4022 4381 4540 518 Size: 359 159 2042 Fragments arranged by size: 2042 1755 892 648 359 159 75 68 45 15 6 MspA1I CmG'CkG Cuts at: 1606 2652 2800 3480 4157 5305 5550 1606 Size: 1046 148 680 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 680 677 245 148 NarI GG'CG_CC Cuts at: 2682 3196 2682 Size: 514 5550 NcoI C'CATG_G Cuts at: 513 1065 2004 2268 2523 513 Size: 552 939 264 255 4054 Fragments arranged by size: 4054 939 552 264 255 NdeI CA'TA_TG Cuts at: 387 387 Size: 6064 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2266 3137 3422 4967 1132 Size: 97 1037 871 285 1545 2229 Fragments arranged by size: 2229 1545 1037 871 285 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 4963 4963 Size: 6064 PflMI CCAn_nnn'nTGG Cuts at: 1108 2652 1108 Size: 1544 4520 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PmlI CAC'GTG Cuts at: 2380 2380 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2447 2952 2447 Size: 505 5559 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6064 PsiI TTA'TAA Cuts at: 3634 3634 Size: 6064 PstI C_TGCA'G Cuts at: 830 2181 2360 3102 3337 3416 830 Size: 1351 179 742 235 79 3478 Fragments arranged by size: 3478 1351 742 235 179 79 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 PvuII CAG'CTG Cuts at: 2800 2800 Size: 6064 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SapI GCTCTTCn'nnn_ Cuts at: 3188 3188 Size: 6064 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SfcI C'TryA_G Cuts at: 826 1045 2177 2278 2356 3098 3333 3412 4547 Size: 219 1132 101 78 742 235 79 1135 Cuts at: 4547 5228 5419 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1132 742 681 235 219 191 101 79 78 SfoI GGC'GCC Cuts at: 2683 3197 2683 Size: 514 5550 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 2025 3391 1872 Size: 153 1366 4545 Fragments arranged by size: 4545 1366 153 SmlI C'TyrA_G Cuts at: 820 1017 1943 3232 3287 4168 5069 5331 820 Size: 197 926 1289 55 881 901 262 1553 Fragments arranged by size: 1553 1289 926 901 881 262 197 55 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 2266 3422 1132 Size: 1134 1156 3774 Fragments arranged by size: 3774 1156 1134 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 2354 1517 Size: 837 5227 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 2523 3503 513 Size: 552 939 264 255 980 3074 Fragments arranged by size: 3074 980 939 552 264 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2029 2183 3004 4091 4108 4261 4446 Size: 1255 449 154 821 1087 17 153 185 Cuts at: 4446 4891 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1087 821 449 445 185 154 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3033 4310 6022 Size: 274 80 33 51 1443 1056 1277 1712 Cuts at: 6022 96 Size: 138 Fragments arranged by size: 1712 1443 1277 1056 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2004 2036 2776 2808 6024 6056 2004 Size: 32 740 32 3216 32 2012 Fragments arranged by size: 3216 2012 740 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2305 2950 1316 Size: 989 645 4430 Fragments arranged by size: 4430 989 645 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XhoI C'TCGA_G Cuts at: 1943 3287 1943 Size: 1344 4720 XmaI C'CCGG_G Cuts at: 1870 2023 3389 1870 Size: 153 1366 4545 Fragments arranged by size: 4545 1366 153 XmnI GAAnn'nnTTC Cuts at: 3227 4193 3227 Size: 966 5098 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I EcoICRI EcoRV FseI NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI