(Circular) (Six-base) MAPSORT of: pFN21AB5856.seq Check: 1168 from: 1 to: 6172 pFN21AB5856 6172 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3982 278 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3515 1937 Size: 1578 4594 Acc65I G'GTAC_C Cuts at: 3493 3493 Size: 6172 AclI AA'CG_TT Cuts at: 1563 4299 4672 1563 Size: 2736 373 3063 Fragments arranged by size: 3063 2736 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2433 3099 4235 5619 5887 1632 Size: 801 666 1136 1384 268 1917 Fragments arranged by size: 1917 1384 1136 801 666 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5975 AflIII A'CryG_T Cuts at: 1203 2668 3385 5071 6048 1203 Size: 1465 717 1686 977 1327 Fragments arranged by size: 1686 1465 1327 977 717 AgeI A'CCGG_T Cuts at: 5003 5003 Size: 6172 AhdI GACnn_n'nnGTC Cuts at: 4901 4901 Size: 6172 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6172 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6172 AlwNI CAG_nnn'CTG Cuts at: 3915 5487 3915 Size: 1572 4600 ApaLI G'TGCA_C Cuts at: 4229 5385 4229 Size: 1156 5016 ApoI r'AATT_y Cuts at: 1493 2212 2290 2357 2632 3290 3464 3481 3707 Size: 719 78 67 275 658 174 17 226 Cuts at: 3707 3859 5968 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 719 658 275 226 174 152 78 67 17 AseI AT'TA_AT Cuts at: 160 1032 2823 4726 160 Size: 872 1791 1903 1606 Fragments arranged by size: 1903 1791 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 AvaI C'yCGr_G Cuts at: 1870 1943 3103 3497 1870 Size: 73 1160 394 4545 Fragments arranged by size: 4545 1160 394 73 BamHI G'GATC_C Cuts at: 3502 3869 3502 Size: 367 5805 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2063 3493 4948 618 Size: 325 217 627 276 1430 1455 1842 Fragments arranged by size: 1842 1455 1430 627 325 276 217 BanII G_rGCy'C Cuts at: 1391 3339 3491 1391 Size: 1948 152 4072 Fragments arranged by size: 4072 1948 152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3021 928 Size: 971 1122 4079 Fragments arranged by size: 4079 1122 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4363 4397 1903 Size: 34 2426 34 3678 Fragments arranged by size: 3678 2426 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2632 4064 5274 2632 Size: 1432 1210 3530 Fragments arranged by size: 3530 1432 1210 BclI T'GATC_A Cuts at: 1548 2269 3220 1548 Size: 721 951 4500 Fragments arranged by size: 4500 951 721 BfrBI ATG'CAT Cuts at: 3007 3007 Size: 6172 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4783 136 Size: 107 122 71 631 605 3111 1525 Fragments arranged by size: 3111 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6167 6167 Size: 6172 BlpI GC'TnA_GC Cuts at: 2786 3589 2786 Size: 803 5369 Bme1580I G_kGCm'C Cuts at: 1790 2066 4233 5389 1790 Size: 276 2167 1156 2573 Fragments arranged by size: 2573 2167 1156 276 BmgBI CAC'GTC Cuts at: 1318 2931 3097 1318 Size: 1613 166 4393 Fragments arranged by size: 4393 1613 166 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3903 4861 442 Size: 416 53 83 399 24 2486 958 1753 Fragments arranged by size: 2486 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2970 3184 3211 4832 1239 Size: 1731 214 27 1621 2579 Fragments arranged by size: 2579 1731 1621 214 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4297 5162 5460 4297 Size: 865 298 5009 Fragments arranged by size: 5009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4835 882 Size: 617 3336 2219 Fragments arranged by size: 3336 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3963 493 Size: 711 2759 2702 Fragments arranged by size: 2759 2702 711 BsaBI GATnn'nnATC Cuts at: 2487 6166 2487 Size: 3679 2493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3979 4361 4996 275 Size: 53 83 186 936 2446 382 635 1451 Fragments arranged by size: 2446 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4605 5003 5277 5424 5835 4605 Size: 398 274 147 411 4942 Fragments arranged by size: 4942 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5947 Fragments arranged by size: 5947 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2003 2429 1186 Size: 817 426 4929 Fragments arranged by size: 4929 817 426 BseYI C'CCAG_C Cuts at: 5375 5871 5375 Size: 496 5676 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5599 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3622 4383 5411 1540 Size: 275 186 1621 761 1028 2301 Fragments arranged by size: 2301 1621 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3339 4233 4318 5389 3339 Size: 894 85 1071 4122 Fragments arranged by size: 4122 1071 894 85 BsmI GAATG_Cn' Cuts at: 3683 3776 3683 Size: 93 6079 BsmBI CGTCTCn'nnnn_ Cuts at: 2460 2460 Size: 6172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 3339 3491 4233 4318 5389 1391 Size: 399 276 1273 152 742 85 1071 2174 Fragments arranged by size: 2174 1273 1071 742 399 276 152 85 BspEI T'CCGG_A Cuts at: 5835 5835 Size: 6172 BspHI T'CATG_A Cuts at: 2427 4061 5791 2427 Size: 1634 1730 2808 Fragments arranged by size: 2808 1730 1634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3527 844 Size: 2683 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4059 1394 Size: 429 2236 3507 Fragments arranged by size: 3507 2236 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4667 4841 65 Size: 1183 3419 174 1396 Fragments arranged by size: 3419 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4816 5003 1700 Size: 110 3006 187 2869 Fragments arranged by size: 3006 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2517 96 Size: 2421 3751 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6172 BssSI C'ACGA_G Cuts at: 4232 5244 4232 Size: 1012 5160 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6172 BstBI TT'CG_AA Cuts at: 4976 4976 Size: 6172 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6172 BstXI CCAn_nnnn'nTGG Cuts at: 3259 3259 Size: 6172 BstYI r'GATC_y Cuts at: 1972 2071 2550 3397 3502 3869 4254 4271 5723 Size: 99 479 847 105 367 385 17 1452 Cuts at: 5723 5809 5821 6167 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 847 479 385 367 346 105 99 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2067 513 Size: 552 939 63 4618 Fragments arranged by size: 4618 939 552 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3163 487 Size: 683 56 1937 3496 Fragments arranged by size: 3496 1937 683 56 BtsI GCAGTG_nn' Cuts at: 3688 4482 4502 3688 Size: 794 20 5358 Fragments arranged by size: 5358 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6172 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3349 3384 529 Size: 35 2785 35 3317 Fragments arranged by size: 3317 2785 35 35 DraI TTT'AAA Cuts at: 3186 3476 3186 Size: 290 5882 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5179 809 Size: 507 3863 1802 Fragments arranged by size: 3863 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3619 4508 6137 Size: 54 1425 50 275 18 1789 889 1629 Cuts at: 6137 8 Size: 43 Fragments arranged by size: 1789 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3619 1537 Size: 275 1807 4090 Fragments arranged by size: 4090 1807 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2967 4102 1353 Size: 1614 1135 3423 Fragments arranged by size: 3423 1614 1135 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4759 5133 5279 387 Size: 1064 138 3170 374 146 1280 Fragments arranged by size: 3170 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3337 3337 Size: 6172 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2433 2970 3099 3184 3211 4235 4832 Size: 393 801 537 129 85 27 1024 597 Cuts at: 4832 5619 5887 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1024 801 787 597 537 393 268 129 85 27 EcoO109I rG'GnC_Cy Cuts at: 2170 2170 Size: 6172 EcoRI G'AATT_C Cuts at: 2212 2632 3481 3859 2212 Size: 420 849 378 4525 Fragments arranged by size: 4525 849 420 378 EcoRV GAT'ATC Cuts at: 2404 2404 Size: 6172 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2759 2904 2759 Size: 145 6027 FspI TGC'GCA Cuts at: 4678 4678 Size: 6172 HaeII r_GCGC'y Cuts at: 1678 5319 1678 Size: 3641 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2966 2998 3321 3353 4813 Size: 32 135 32 1394 32 323 32 1460 Cuts at: 4813 4845 4887 4919 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1460 1394 323 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3516 3762 669 Size: 988 281 1578 246 3079 Fragments arranged by size: 3079 1578 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6172 HpaI GTT'AAC Cuts at: 3762 3762 Size: 6172 KpnI G_GTAC'C Cuts at: 3497 3497 Size: 6172 MfeI C'AATT_G Cuts at: 2112 3771 2112 Size: 1659 4513 MluI A'CGCG_T Cuts at: 6048 6048 Size: 6172 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2302 5286 5470 1305 Size: 997 2984 184 2007 Fragments arranged by size: 2984 2007 997 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4350 Fragments arranged by size: 4350 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2277 2513 4130 4489 4648 518 Size: 648 1111 236 1617 359 159 2042 Fragments arranged by size: 2042 1617 1111 648 359 236 159 MspA1I CmG'CkG Cuts at: 1606 2069 3255 3588 4265 5413 5658 1606 Size: 463 1186 333 677 1148 245 2120 Fragments arranged by size: 2120 1186 1148 677 463 333 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4681 Fragments arranged by size: 4681 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6172 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6172 NotI GC'GGCC_GC Cuts at: 3619 3619 Size: 6172 NsiI A_TGCA'T Cuts at: 3009 3009 Size: 6172 NspI r_CATG'y Cuts at: 1132 1229 2672 3007 3389 3530 5075 1132 Size: 97 1443 335 382 141 1545 2229 Fragments arranged by size: 2229 1545 1443 382 335 141 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6172 PciI A'CATG_T Cuts at: 2668 3385 5071 2668 Size: 717 1686 3769 Fragments arranged by size: 3769 1686 717 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6172 PfoI T'CCnGG_A Cuts at: 3022 3022 Size: 6172 PmeI GTTT'AAAC Cuts at: 3476 3476 Size: 6172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3121 3153 4241 4273 5754 5786 3121 Size: 32 1088 32 1481 32 3507 Fragments arranged by size: 3507 1481 1088 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2170 2170 Size: 6172 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6172 PsiI TTA'TAA Cuts at: 3742 3742 Size: 6172 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2722 2754 2722 Size: 32 6140 PstI C_TGCA'G Cuts at: 830 3524 830 Size: 2694 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6172 PvuII CAG'CTG Cuts at: 3255 3255 Size: 6172 SacI G_AGCT'C Cuts at: 3339 3339 Size: 6172 SacII CC_GC'GG Cuts at: 2070 2070 Size: 6172 SalI G'TCGA_C Cuts at: 1936 3514 1936 Size: 1578 4594 SapI GCTCTTCn'nnn_ Cuts at: 2967 2967 Size: 6172 SbfI CC_TGCA'GG Cuts at: 3524 3524 Size: 6172 ScaI AGT'ACT Cuts at: 2642 4420 6132 2642 Size: 1778 1712 2682 Fragments arranged by size: 2682 1778 1712 SfcI C'TryA_G Cuts at: 826 1045 3520 4655 5336 5527 826 Size: 219 2475 1135 681 191 1471 Fragments arranged by size: 2475 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6172 SmaI CCC'GGG Cuts at: 1872 3105 3499 1872 Size: 1233 394 4545 Fragments arranged by size: 4545 1233 394 SmlI C'TyrA_G Cuts at: 820 1017 1943 4276 5177 5439 820 Size: 197 926 2333 901 262 1553 Fragments arranged by size: 2333 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3963 493 Size: 3470 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6172 SphI G_CATG'C Cuts at: 1132 3007 3530 1132 Size: 1875 523 3774 Fragments arranged by size: 3774 1875 523 SspI AAT'ATT Cuts at: 5 52 2242 2263 2850 3088 4096 5 Size: 47 2190 21 587 238 1008 2081 Fragments arranged by size: 2190 2081 1008 587 238 47 21 StuI AGG'CCT Cuts at: 1517 1517 Size: 6172 StyI C'CwwG_G Cuts at: 513 1065 2004 3611 513 Size: 552 939 1607 3074 Fragments arranged by size: 3074 1607 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4199 4216 4369 4554 4999 325 Size: 1255 2619 17 153 185 445 1498 Fragments arranged by size: 2619 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2517 2640 4418 Size: 274 80 33 51 1443 540 123 1778 Cuts at: 4418 6130 96 Size: 1712 138 Fragments arranged by size: 1778 1712 1443 540 274 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6132 6164 6132 Size: 32 6140 Tth111I GACn'n_nGTC Cuts at: 1316 3098 1316 Size: 1782 4390 XbaI T'CTAG_A Cuts at: 3508 3508 Size: 6172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3167 3167 Size: 6172 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6172 XmaI C'CCGG_G Cuts at: 1870 3103 3497 1870 Size: 1233 394 4545 Fragments arranged by size: 4545 1233 394 XmnI GAAnn'nnTTC Cuts at: 3087 3208 4301 3087 Size: 121 1093 4958 Fragments arranged by size: 4958 1093 121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3980 276 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SanDI SexAI SfiI SfoI SrfI SwaI