Restriction map for
pFN21AB7522
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BalI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI Bsp1407I BspDI BspEI BspMI
BsrBI BsrGI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstXI BstZ17I Bsu36I CelII
Cfr13I ClaI CpoI DraI DraII
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvaI
MwoI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI Sau96I SbfI
ScaI ScrFI SexAI SfaNI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TfiI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 10 CGCG CG^CG
AcyI 31 GGCGCC G[GA]^CG[CT]C
AflII 595 CTTAAG C^TTAAG
AflIII 543 ACATGT A^C[GA][CT]GT
AvaI 527 CTCGAG C^[CT]CG[GA]G
BamHI 680 GGATCC G^GATCC
BanI 31 GGCGCC G^G[CT][GA]CC
BbeI 31 GGCGCC GGCGC^C
BbvI 355 GCTGC GCAGC(8/12)
BfrI 595 CTTAAG C^TTAAG
BsaHI 31 GGCGCC G[GA]^CG[CT]C
BsiYI 5 CCGAGCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 5 CCGAGCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 343 GAATGC GAATGC(1/-1)
BsoBI 527 CTCGAG C^[CT]CG[GA]G
BspHI 195 TCATGA T^CATGA
BspLU11I 543 ACATGT A^CATGT
BsrDI 87 GCAATG GCAATG(2/0)
BsrFI 28 ACCGGC [GA]^CCGG[CT]
BstF5I 241 CATCC GGATG(2/0)
BstUI 10 CGCG CG^CG
Cac8I 522 GCGAGC GC[ACGT]^[ACGT]GC
Cfr10I 28 ACCGGC [GA]^CCGG[CT]
DrdI 153 GACTGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2 TGGCCG [CT]^GGCC[GA]
FauI 11 GCGGG CCCGC(4/6)
Fnu4HI 355 GCTGC GC^[ACGT]GC
FokI 241 CATCC GGATG(9/13)
HaeII 31 GGCGCC [GA]GCGC^[CT]
HaeIII 3 GGCC GG^CC
HapII 29 CCGG C^CGG
Hin1I 31 GGCGCC G[GA]^CG[CT]C
HpaII 29 CCGG C^CGG
HphI 112 GGTGA GGTGA(8/7)
ItaI 355 GCTGC GC^[ACGT]GC
KasI 31 GGCGCC G^GCGCC
MspI 29 CCGG C^CGG
MvnI 10 CGCG CG^CG
NarI 31 GGCGCC GG^CGCC
PaeR7I 527 CTCGAG C^TCGAG
RcaI 195 TCATGA T^CATGA
SfoI 31 GGCGCC GGC^GCC
TseI 355 GCTGC G^C[AT]GC
XhoI 527 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 11 GCGG CCGC(-3/-1)
129 GCGG
BanII 294 GAGCTC G[GA]GC[CT]^C
524 GAGCTC
BbsI 511 GAAGAC GAAGAC(2/6)
402 GTCTTC
BfaI 58 CTAG C^TAG
233 CTAG
BpuAI 511 GAAGAC GAAGAC(2/6)
402 GTCTTC
BsrI 359 CCAGT ACTGG(1/-1)
412 CCAGT
BstYI 180 GGATCT [GA]^GATC[CT]
680 GGATCC
CfoI 9 GCGC GCG^C
32 GCGC
DdeI 155 CTGAG C^T[ACGT]AG
290 CTGAG
DpnII 181 GATC ^GATC
681 GATC
EarI 107 GAAGAG CTCTTC(1/4)
397 GAAGAG
HhaI 9 GCGC GCG^C
32 GCGC
HinP1I 9 GCGC G^CGC
32 GCGC
HinfI 141 GACTC G^A[ACGT]TC
400 GAGTC
Ksp632I 107 GAAGAG CTCTTC(1/4)
397 GAAGAG
MaeI 58 CTAG C^TAG
233 CTAG
MboI 181 GATC ^GATC
681 GATC
MflI 180 GGATCT [GA]^GATC[CT]
680 GGATCC
MslI 45 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
83 CACAGCAATG
MspA1I 353 CAGCTG C[AC]G^C[GT]G
430 CAGCTG
NdeII 181 GATC ^GATC
681 GATC
PleI 141 GACTC GAGTC(4/5)
400 GAGTC
PvuII 353 CAGCTG CAG^CTG
430 CAGCTG
SacI 294 GAGCTC GAGCT^C
524 GAGCTC
Sau3AI 181 GATC ^GATC
681 GATC
SspI 74 AATATT AAT^ATT
190 AATATT
Tsp45I 81 GTCAC ^GT[GC]AC
743 GTGAC
Tsp509I 454 AATT ^AATT
657 AATT
XhoII 180 GGATCT [GA]^GATC[CT]
680 GGATCC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AfaI AluI AlwI AspHI BmyI
BsiHKAI Bsp1286I MaeIII MboII MnlI
MseI NlaIII NlaIV NspI RsaI
SmlI TaqI Tru9I TspRI TthHB8I