Restriction map for
pFN21AB7522
(Circular) (Six-base) MAPSORT of: pFN21AB7522.seq Check: 3104 from: 1 to: 5452
pFN21AB7522 5452 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3262 278
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2795 1937
Size: 858 4594
Acc65I G'GTAC_C
Cuts at: 2773 2773
Size: 5452
AclI AA'CG_TT
Cuts at: 1563 3579 3952 1563
Size: 2016 373 3063
Fragments arranged by size:
3063 2016 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2208 2663 2729 3515 4899 5167 1632
Size: 576 455 66 786 1384 268 1917
Fragments arranged by size:
1917 1384 786 576 455 268 66
AflII C'TTAA_G
Cuts at: 820 1017 2600 820
Size: 197 1583 3672
Fragments arranged by size:
3672 1583 197
AflIII A'CryG_T
Cuts at: 1203 2548 4351 5328 1203
Size: 1345 1803 977 1327
Fragments arranged by size:
1803 1345 1327 977
AgeI A'CCGG_T
Cuts at: 4283 4283
Size: 5452
AhdI GACnn_n'nnGTC
Cuts at: 4181 4181
Size: 5452
AleI CACnn'nnGTG
Cuts at: 1166 2054 1166
Size: 888 4564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5452
AlwNI CAG_nnn'CTG
Cuts at: 3195 4767 3195
Size: 1572 3880
ApaLI G'TGCA_C
Cuts at: 3509 4665 3509
Size: 1156 4296
ApoI r'AATT_y
Cuts at: 1493 2761 2987 3139 5248 1493
Size: 1268 226 152 2109 1697
Fragments arranged by size:
2109 1697 1268 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4006 160
Size: 872 2974 1606
Fragments arranged by size:
2974 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
AvaI C'yCGr_G
Cuts at: 1870 1943 2532 2777 1870
Size: 73 589 245 4545
Fragments arranged by size:
4545 589 245 73
BamHI G'GATC_C
Cuts at: 2685 2782 3149 2685
Size: 97 367 4988
Fragments arranged by size:
4988 367 97
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2773 4228 618
Size: 325 217 627 249 737 1455 1842
Fragments arranged by size:
1842 1455 737 627 325 249 217
BanII G_rGCy'C
Cuts at: 1391 2303 2533 2771 1391
Size: 912 230 238 4072
Fragments arranged by size:
4072 912 238 230
BbeI G_GCGC'C
Cuts at: 2040 2040
Size: 5452
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2400 2523 928
Size: 971 501 123 3857
Fragments arranged by size:
3857 971 501 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3643 3677 1903
Size: 34 1706 34 3678
Fragments arranged by size:
3678 1706 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3344 4554 3344
Size: 1210 4242
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4063 136
Size: 107 122 71 631 605 2391 1525
Fragments arranged by size:
2391 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5447 5447
Size: 5452
BlpI GC'TnA_GC
Cuts at: 2869 2869
Size: 5452
Bme1580I G_kGCm'C
Cuts at: 1790 3513 4669 1790
Size: 1723 1156 2573
Fragments arranged by size:
2573 1723 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5452
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3183 4141 442
Size: 416 53 83 399 24 1766 958 1753
Fragments arranged by size:
1766 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4112 1239
Size: 2873 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2133 3577 4442 4740 2133
Size: 1444 865 298 2845
Fragments arranged by size:
2845 1444 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4115 882
Size: 617 2616 2219
Fragments arranged by size:
2616 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3243 493
Size: 711 2039 2702
Fragments arranged by size:
2702 2039 711
BsaBI GATnn'nnATC
Cuts at: 5446 5446
Size: 5452
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 3259 3641 4276
Size: 53 83 186 936 504 1222 382 635
Cuts at: 4276 275
Size: 1451
Fragments arranged by size:
1451 1222 936 635 504 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3885 4283 4557 4704 5115 3885
Size: 398 274 147 411 4222
Fragments arranged by size:
4222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5227
Fragments arranged by size:
5227 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5452
BseYI C'CCAG_C
Cuts at: 4655 5151 4655
Size: 496 4956
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4879
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2902 3663 4691 1540
Size: 275 186 901 761 1028 2301
Fragments arranged by size:
2301 1028 901 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2236 2303 2533 3513 3598 4669 2236
Size: 67 230 980 85 1071 3019
Fragments arranged by size:
3019 1071 980 230 85 67
BsmI GAATG_Cn'
Cuts at: 2354 2963 3056 2354
Size: 609 93 4750
Fragments arranged by size:
4750 609 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2236 2303 2533 2771 3513 3598 4669
Size: 399 446 67 230 238 742 85 1071
Cuts at: 4669 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 446 399 238 230 85
67
BspEI T'CCGG_A
Cuts at: 5115 5115
Size: 5452
BspHI T'CATG_A
Cuts at: 2200 3341 5071 2200
Size: 1141 1730 2581
Fragments arranged by size:
2581 1730 1141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2807 844
Size: 1963 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3339 1394
Size: 429 1516 3507
Fragments arranged by size:
3507 1516 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2099 3947 4121 65
Size: 1183 851 1848 174 1396
Fragments arranged by size:
1848 1396 1183 851 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 4096 4283 1700
Size: 110 223 2063 187 2869
Fragments arranged by size:
2869 2063 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5452
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5452
BssSI C'ACGA_G
Cuts at: 3512 4524 3512
Size: 1012 4440
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5452
BstBI TT'CG_AA
Cuts at: 4256 4256
Size: 5452
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5452
BstYI r'GATC_y
Cuts at: 1972 2185 2685 2782 3149 3534 3551 5003 5089
Size: 213 500 97 367 385 17 1452 86
Cuts at: 5089 5101 5447 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 500 385 367 346 213 97
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3961
Fragments arranged by size:
3961 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4713
Fragments arranged by size:
4713 683 56
BtsI GCAGTG_nn'
Cuts at: 2968 3762 3782 2968
Size: 794 20 4638
Fragments arranged by size:
4638 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5417
DraI TTT'AAA
Cuts at: 2756 2756
Size: 5452
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2164 4459 809
Size: 507 848 2295 1802
Fragments arranged by size:
2295 1802 848 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2899 3788
Size: 54 1425 50 275 18 177 892 889
Cuts at: 3788 5417 8
Size: 1629 43
Fragments arranged by size:
1629 1425 892 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2899 1537
Size: 275 1087 4090
Fragments arranged by size:
4090 1087 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2107 2397 3382 1353
Size: 754 290 985 3423
Fragments arranged by size:
3423 985 754 290
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4039 4413 4559 387
Size: 1064 138 2450 374 146 1280
Fragments arranged by size:
2450 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2301 2531 2301
Size: 230 5222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2663 2729 3515 4112 4899 5167
Size: 393 576 455 66 786 597 787 268
Cuts at: 5167 1239
Size: 1524
Fragments arranged by size:
1524 787 786 597 576 455 393 268
66
EcoRI G'AATT_C
Cuts at: 2761 3139 2761
Size: 378 5074
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 5452
FspI TGC'GCA
Cuts at: 3958 3958
Size: 5452
HaeII r_GCGC'y
Cuts at: 1678 2040 4599 1678
Size: 362 2559 2531
Fragments arranged by size:
2559 2531 362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2300 2320 2332 4093
Size: 32 135 32 716 12 20 12 1761
Cuts at: 4093 4125 4167 4199 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1761 716 135 42 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2796 3042 669
Size: 988 281 858 246 3079
Fragments arranged by size:
3079 988 858 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5452
HpaI GTT'AAC
Cuts at: 3042 3042
Size: 5452
KasI G'GCGC_C
Cuts at: 2036 2036
Size: 5452
KpnI G_GTAC'C
Cuts at: 2777 2777
Size: 5452
MfeI C'AATT_G
Cuts at: 3051 3051
Size: 5452
MluI A'CGCG_T
Cuts at: 5328 5328
Size: 5452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2248 4566 4750 1305
Size: 943 2318 184 2007
Fragments arranged by size:
2318 2007 943 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3630
Fragments arranged by size:
3630 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 2092 3410 3769 3928 518
Size: 648 888 38 1318 359 159 2042
Fragments arranged by size:
2042 1318 888 648 359 159 38
MspA1I CmG'CkG
Cuts at: 1606 2360 2437 2868 3545 4693 4938 1606
Size: 754 77 431 677 1148 245 2120
Fragments arranged by size:
2120 1148 754 677 431 245 77
NarI GG'CG_CC
Cuts at: 2037 2037
Size: 5452
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3961
Fragments arranged by size:
3961 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5452
NotI GC'GGCC_GC
Cuts at: 2899 2899
Size: 5452
NspI r_CATG'y
Cuts at: 1132 1229 2380 2552 2563 2810 4355 1132
Size: 97 1151 172 11 247 1545 2229
Fragments arranged by size:
2229 1545 1151 247 172 97 11
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5452
PciI A'CATG_T
Cuts at: 2548 4351 2548
Size: 1803 3649
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5452
PmeI GTTT'AAAC
Cuts at: 2756 2756
Size: 5452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 5034 5066 3521
Size: 32 1481 32 3907
Fragments arranged by size:
3907 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5452
PsiI TTA'TAA
Cuts at: 3022 3022
Size: 5452
PspXI vC'TCGA_Gb
Cuts at: 2532 2532
Size: 5452
PstI C_TGCA'G
Cuts at: 830 2804 830
Size: 1974 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5452
PvuII CAG'CTG
Cuts at: 2360 2437 2360
Size: 77 5375
SacI G_AGCT'C
Cuts at: 2303 2533 2303
Size: 230 5222
SalI G'TCGA_C
Cuts at: 1936 2794 1936
Size: 858 4594
SbfI CC_TGCA'GG
Cuts at: 2804 2804
Size: 5452
ScaI AGT'ACT
Cuts at: 3700 5412 3700
Size: 1712 3740
SfcI C'TryA_G
Cuts at: 826 1045 2800 3935 4616 4807 826
Size: 219 1755 1135 681 191 1471
Fragments arranged by size:
1755 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2038 2038
Size: 5452
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5452
SmaI CCC'GGG
Cuts at: 1872 2779 1872
Size: 907 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2148 2532 2600 3556 4457 4719
Size: 197 926 205 384 68 956 901 262
Cuts at: 4719 820
Size: 1553
Fragments arranged by size:
1553 956 926 901 384 262 205 197
68
SnaBI TAC'GTA
Cuts at: 493 3243 493
Size: 2750 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5452
SphI G_CATG'C
Cuts at: 1132 2810 1132
Size: 1678 3774
SspI AAT'ATT
Cuts at: 5 52 2081 2197 3376 5
Size: 47 2029 116 1179 2081
Fragments arranged by size:
2081 2029 1179 116 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5452
StyI C'CwwG_G
Cuts at: 513 1065 2004 2891 513
Size: 552 939 887 3074
Fragments arranged by size:
3074 939 887 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3479 3496 3649 3834 4279 325
Size: 1255 1899 17 153 185 445 1498
Fragments arranged by size:
1899 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2718 3698 5410
Size: 274 80 33 51 1443 741 980 1712
Cuts at: 5410 96
Size: 138
Fragments arranged by size:
1712 1443 980 741 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5412 5444 5412
Size: 32 5420
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5452
XbaI T'CTAG_A
Cuts at: 2788 2788
Size: 5452
XhoI C'TCGA_G
Cuts at: 1943 2532 1943
Size: 589 4863
XmaI C'CCGG_G
Cuts at: 1870 2777 1870
Size: 907 4545
XmnI GAAnn'nnTTC
Cuts at: 3581 3581
Size: 5452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3260 276
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI