Restriction map for
pFN21AE0779
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AocI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaI BsaWI BseAI
BsgI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BsrI Bst1107I
BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MaeI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Alw44I 478 GTGCAC G^TGCAC
AlwNI 151 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 946 AGCGCT AGC^GCT
ApaLI 478 GTGCAC G^TGCAC
BanI 977 GGCGCC G^G[CT][GA]CC
BbeI 977 GGCGCC GGCGC^C
BbvCI 868 CCTCAGC CCTCAGC(-5/-2)
BsaBI 954 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 896 CGGCCG CG[GA][CT]^CG
BssSI 618 CACGAG CACGAG(-5/-1)
BstEII 234 GGTCACC G^GT[ACGT]ACC
BstPI 234 GGTCACC G^GT[ACGT]ACC
DpnII 954 GATC ^GATC
EagI 896 CGGCCG C^GGCCG
EclXI 896 CGGCCG C^GGCCG
Eco47III 946 AGCGCT AGC^GCT
Eco52I 896 CGGCCG C^GGCCG
EcoO65I 234 GGTCACC G^GT[ACGT]ACC
EcoT14I 452 CCAAGG C^C[AT][AT]GG
HinfI 382 GAGTC G^A[ACGT]TC
KasI 977 GGCGCC G^GCGCC
MamI 954 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 954 GATC ^GATC
NarI 977 GGCGCC GG^CGCC
NdeII 954 GATC ^GATC
PleI 382 GAGTC GAGTC(4/5)
PshAI 153 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 496 CAGCTG CAG^CTG
SapI 756 GCTCTTC GCTCTTC(1/4)
Sau3AI 954 GATC ^GATC
SfaNI 377 GCATC GCATC(5/9)
SfcI 396 CTACAG C^T[GA][CT]AG
SfiI 888 GGCCCCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 977 GGCGCC GGC^GCC
SmaI 857 CCCGGG CCC^GGG
StuI 622 AGGCCT AGG^CCT
StyI 452 CCAAGG C^C[AT][AT]GG
TaqI 380 TCGA T^CGA
TthHB8I 380 TCGA T^CGA
XcmI 207 CCAGGGTCGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 857 CCCGGG C^CCGGG
XmaI 857 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BcgI 37 GCAGGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
371 GCATTGGCATCG
BglI 493 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
889 GCCCCCGCGGC
BsrBI 697 CCGCTC CCGCTC(-3/-3)
1019 GAGCGG
EarI 757 CTCTTC CTCTTC(1/4)
1027 GAAGAG
EcoNI 201 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
798 CCTGGCCGAGG
HphI 236 TCACC GGTGA(8/7)
930 TCACC
Ksp632I 757 CTCTTC CTCTTC(1/4)
1027 GAAGAG
MaeII 292 ACGT A^CGT
563 ACGT
MaeIII 235 GTCAC ^GT[ACGT]AC
358 GTGAC
MboII 1027 GAAGA GAAGA(8/7)
758 TCTTC
NaeI 27 GCCGGC GCC^GGC
587 GCCGGC
NgoMIV 27 GCCGGC G^CCGGC
587 GCCGGC
PpuMI 428 GGGACCT [GA]G^G[AT]CC[CT]
545 GGGACCT
Tsp45I 235 GTCAC ^GT[GC]AC
358 GTGAC
TspRI 266 CACTG CA[GC]TG[ACGT][ACGT]^
1013 CAGTG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
KspI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
RsaI SacI SacII Sau96I ScrFI
TseI